Restriction map for pF1KE0112
 (Linear) (Six-base) MAPSORT of: KIEE0112.seq  Check: 9601  from: 1  to: 585



KIEE0112.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:10  ..

AfeI AGC'GCT

Cuts at:      0     570     585 
   Size:        570      15
 
ApaI G_GGCC'C

Cuts at:      0     512     585 
   Size:        512      73
 
ApoI r'AATT_y

Cuts at:      0     271     585 
   Size:        271     314
 
AvaI C'yCGr_G

Cuts at:      0      26     537     585 
   Size:         26     511      48

  Fragments arranged by size: 

                 511      48      26
 
BanI G'GyrC_C

Cuts at:      0     394     585 
   Size:        394     191
 
BanII G_rGCy'C

Cuts at:      0      16     512     585 
   Size:         16     496      73

  Fragments arranged by size: 

                 496      73      16
 
BbvCI CC'TCA_GC

Cuts at:      0     221     585 
   Size:        221     364
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     397     431     585 
   Size:        397      34     154

  Fragments arranged by size: 

                 397     154      34
 
BglI GCCn_nnn'nGGC

Cuts at:      0      49     472     585 
   Size:         49     423     113

  Fragments arranged by size: 

                 423     113      49
 
BlpI GC'TnA_GC

Cuts at:      0     572     585 
   Size:        572      13
 
Bme1580I G_kGCm'C

Cuts at:      0     415     512     585 
   Size:        415      97      73

  Fragments arranged by size: 

                 415      97      73
 
BmgBI CAC'GTC

Cuts at:      0      36     585 
   Size:         36     549
 
Bpu10I CC'TnA_GC

Cuts at:      0     221     419     585 
   Size:        221     198     166

  Fragments arranged by size: 

                 221     198     166
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     244     585 
   Size:        244     341
 
BsaAI yAC'GTr

Cuts at:      0      99     585 
   Size:         99     486
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      61     345     585 
   Size:         61     284     240

  Fragments arranged by size: 

                 284     240      61
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     506     585 
   Size:        506      79
 
BsiHKAI G_wGCw'C

Cuts at:      0      16     585 
   Size:         16     569
 
Bsp1286I G_dGCh'C

Cuts at:      0      16     415     512     585 
   Size:         16     399      97      73

  Fragments arranged by size: 

                 399      97      73      16
 
BsrBI CCG'CTC

Cuts at:      0     302     585 
   Size:        302     283
 
BsrDI GCAATG_nn'

Cuts at:      0     114     585 
   Size:        114     471
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0      91     585 
   Size:         91     494
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     200     585 
   Size:        200     385
 
BtsI GCAGTG_nn'

Cuts at:      0     413     585 
   Size:        413     172
 
EaeI y'GGCC_r

Cuts at:      0      81      93     585 
   Size:         81      12     492

  Fragments arranged by size: 

                 492      81      12
 
EarI CTCTTCn'nnn_

Cuts at:      0     365     494     585 
   Size:        365     129      91

  Fragments arranged by size: 

                 365     129      91
 
EcoICRI GAG'CTC

Cuts at:      0      14     585 
   Size:         14     571
 
EcoO109I rG'GnC_Cy

Cuts at:      0     237     508     509     585 
   Size:        237     271       1      76

  Fragments arranged by size: 

                 271     237      76       1
 
HaeII r_GCGC'y

Cuts at:      0      62     572     585 
   Size:         62     510      13

  Fragments arranged by size: 

                 510      62      13
 
MscI TGG'CCA

Cuts at:      0      95     585 
   Size:         95     490
 
PmlI CAC'GTG

Cuts at:      0      99     585 
   Size:         99     486
 
PpuMI rG'GwC_Cy

Cuts at:      0     237     585 
   Size:        237     348
 
PspOMI G'GGCC_C

Cuts at:      0     508     585 
   Size:        508      77
 
PstI C_TGCA'G

Cuts at:      0     403     585 
   Size:        403     182
 
SacI G_AGCT'C

Cuts at:      0      16     585 
   Size:         16     569
 
SapI GCTCTTCn'nnn_

Cuts at:      0     365     585 
   Size:        365     220
 
SfcI C'TryA_G

Cuts at:      0     399     518     585 
   Size:        399     119      67

  Fragments arranged by size: 

                 399     119      67
 
SmaI CCC'GGG

Cuts at:      0      28     585 
   Size:         28     557
 
StyI C'CwwG_G

Cuts at:      0      17     125     227     245     467     585 
   Size:         17     108     102      18     222     118

  Fragments arranged by size: 

                 222     118     108     102      18      17
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     375     585 
   Size:        375     210
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     318     350     585 
   Size:        318      32     235

  Fragments arranged by size: 

                 318     235      32
 
XmaI C'CCGG_G

Cuts at:      0      26     585 
   Size:         26     559
 
XmnI GAAnn'nnTTC

Cuts at:      0     271     289     585 
   Size:        271      18     296

  Fragments arranged by size: 

                 296     271      18
 

 Enzymes that do cut:

     AfeI     ApaI     ApoI     AvaI     BanI    BanII    BbvCI     BcgI
     BglI     BlpI Bme1580I    BmgBI   Bpu10I     BsaI    BsaAI    BseRI
     BsgI  BsiHKAI Bsp1286I    BsrBI    BsrDI    BstXI    BtgZI     BtsI
     EaeI     EarI  EcoICRI EcoO109I    HaeII     MscI     PmlI    PpuMI
   PspOMI     PstI     SacI     SapI     SfcI     SmaI     StyI    TaqII
     TstI     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI    ApaLI     AscI
     AseI    AsiSI    AvrII     BaeI    BamHI     BbeI     BbsI    BciVI
     BclI    BfrBI    BglII     BmrI     BmtI     BplI     BpmI    BpuEI
    BsaBI    BsaHI    BsaWI    BsaXI    BseYI    BsiEI    BsiWI     BsmI
    BsmBI    BspEI    BspHI    BspMI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstYI  BstZ17I   Bsu36I     BtgI     ClaI
    CspCI     DraI   DraIII     DrdI     EagI     EciI  Eco57MI    EcoNI
    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    Hin4I   HincII
  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MslI
   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI     PfoI
     PmeI     PpiI    PshAI     PsiI    PspXI     PsrI     PvuI    PvuII
    RsrII    SacII     SalI    SanDI     SbfI     ScaI    SexAI     SfiI
     SfoI     SgfI    SgrAI     SmlI    SnaBI     SpeI     SphI     SrfI
     SspI     StuI     SwaI     TatI  Tth111I     XbaI     XcmI     XhoI
     ZraI
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