Restriction map for pF1KE0124
##################################
# NO CUT ENZYMES (  164 enzymes) #
##################################

AatII       Acc65I      AccI        AcyI        AflII       
AflIII      AgeI        AhdI        Alw44I      AlwNI       
AocI        Aor51HI     ApaI        ApaLI       AscI        
AseI        AsnI        Asp718I     AspEI       AspHI       
AspI        AvaI        AviII       AvrII       BalI        
BanI        BanII       BbeI        BbrPI       BbsI        
BbvCI       BcgI        BciVI       BclI        BcnI        
BfrI        BglI        BlnI        BmyI        BpmI        
BpuAI       BsaAI       BsaBI       BsaHI       BsaI        
BsiEI       BsiHKAI     BsiWI       BsmBI       BsmI        
BsoBI       Bsp1286I    Bsp1407I    BspDI       BspHI       
BspLU11I    BsrFI       BsrGI       BssHII      Bst1107I    
BstBI       BstEII      BstPI       BstXI       BstZ17I     
Bsu36I      Cfr10I      ClaI        CpoI        DraII       
DrdI        DsaI        EaeI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      Eco81I      EcoNI       
EcoO109I    EcoO65I     EcoRI       EcoRV       EcoT14I     
EcoT22I     FbaI        FseI        FspI        HaeII       
HgaI        Hin1I       HincII      HindII      HindIII     
HinfI       HpaI        KasI        KpnI        KspI        
MaeIII      MamI        MfeI        MluI        MluNI       
MscI        MspA1I      MunI        NaeI        NarI        
NciI        NcoI        NdeI        NgoMIV      NheI        
NotI        NruI        NsiI        NspV        PacI        
PaeR7I      PflFI       PinAI       PleI        PmaCI       
PmeI        PmlI        PpuMI       PshAI       PshBI       
PstI        PvuI        PvuII       RcaI        RsrII       
SacI        SacII       SalI        SbfI        ScaI        
SexAI       SfiI        SfoI        SfuI        SgrAI       
SmaI        SmlI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       SspI        StuI        StyI        
SwaI        TfiI        Tsp45I      Tth111I     XbaI        
XcmI        XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   31 enzymes) #
###################################

AccII         103  CGCG                 CG^CG
AccIII         86  TCCGGA               T^CCGGA
AclI          385  AACGTT               AA^CGTT
Asp700I       337  GAATATATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaII          71  GGACC                G^G[AT]CC
BamHI         643  GGATCC               G^GATCC
BfaI          415  CTAG                 C^TAG
BglII         560  AGATCT               A^GATCT
BlpI          692  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I      692  GCTCAGC              GC^T[ACGT]AGC
BsaWI          86  TCCGGA               [AT]^CCGG[AT]
BseAI          86  TCCGGA               T^CCGGA
BspEI          86  TCCGGA               T^CCGGA
BsrBI         251  GAGCGG               CCGCTC(-3/-3)
BsrDI         189  CATTGC               GCAATG(2/0)
BstAPI         93  GCACACAGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI         103  CGCG                 CG^CG
CelII         692  GCTCAGC              GC^T[ACGT]AGC
CfoI          102  GCGC                 GCG^C
DdeI          693  CTCAG                C^T[ACGT]AG
DraIII         94  CACACAGTG            CAC[ACGT][ACGT][ACGT]^GTG
HhaI          102  GCGC                 GCG^C
HinP1I        102  GCGC                 G^CGC
MaeI          415  CTAG                 C^TAG
MroI           86  TCCGGA               T^CCGGA
MvnI          103  CGCG                 CG^CG
NspI          275  ACATGC               [GA]CATG^[CT]
PflMI         264  CCAATTCATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Psp1406I      385  AACGTT               AA^CGTT
Van91I        264  CCAATTCATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI          337  GAATATATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   24 enzymes) #
###################################

AcsI          313  AAATTT               [GA]^AATT[CT]
              466  AAATTC              
AfaI          751  GTAC                 GT^AC
              978  GTAC                
ApoI          313  AAATTT               [GA]^AATT[CT]
              466  AAATTC              
BseRI         539  CTCCTC               GAGGAG(10/8)
              937  GAGGAG              
BsgI          611  GTGCAG               GTGCAG(16/14)
              868  GTGCAG              
BsmFI          70  GGGAC                GGGAC(10/14)
               24  GTCCC               
BsrI          403  CCAGT                ACTGG(1/-1)
              686  CCAGT               
BssSI         907  CACGAG               CACGAG(-5/-1)
              865  CTCGTG              
BstYI         560  AGATCT               [GA]^GATC[CT]
              643  GGATCC              
Cac8I         363  GCAAGC               GC[ACGT]^[ACGT]GC
              942  GCAAGC              
DraI          216  TTTAAA               TTT^AAA
              352  TTTAAA              
FauI          963  CCCGC                CCCGC(4/6)
              104  GCGGG               
HapII          87  CCGG                 C^CGG
              928  CCGG                
HpaII          87  CCGG                 C^CGG
              928  CCGG                
HphI          553  GGTGA                GGTGA(8/7)
              585  TCACC               
MaeII         386  ACGT                 A^CGT
              426  ACGT                
MflI          560  AGATCT               [GA]^GATC[CT]
              643  GGATCC              
MslI          225  CACAAAAGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              270  CATGGACATG          
MspI           87  CCGG                 C^CGG
              928  CCGG                
NlaIV          70  GGGACC               GG[ACGT]^[ACGT]CC
              643  GGATCC              
RsaI          751  GTAC                 GT^AC
              978  GTAC                
SapI          922  GAAGAGC              GCTCTTC(1/4)
              279  GCTCTTC             
SfcI          117  CTACAG               C^T[GA][CT]AG
              506  CTGTAG              
XhoII         560  AGATCT               [GA]^GATC[CT]
              643  GGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   39 enzymes) #
#########################################

AciI        AluI        AlwI        BbvI        BsaJI       
BsiYI       BslI        BsmAI       BspMI       BssKI       
BstF5I      BstNI       Cfr13I      DpnII       EarI        
EcoRII      Fnu4HI      FokI        HaeIII      ItaI        
Ksp632I     MboI        MboII       MnlI        MseI        
MvaI        MwoI        NdeII       NlaIII      Sau3AI      
Sau96I      ScrFI       SfaNI       TaqI        Tru9I       
TseI        Tsp509I     TspRI       TthHB8I     
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