Restriction map for pF1KB6529
 (Linear) (Six-base) MAPSORT of: KIBB6529.seq  Check: 762  from: 1  to: 1095



KIBB6529.orf

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:47  ..

AatII G_ACGT'C

Cuts at:      0      41    1095 
   Size:         41    1054
 
Acc65I G'GTAC_C

Cuts at:      0     560    1095 
   Size:        560     535
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     247     472     511    1029    1095 
   Size:        247     225      39     518      66

  Fragments arranged by size: 

                 518     247     225      66      39
 
AflII C'TTAA_G

Cuts at:      0     412    1095 
   Size:        412     683
 
AflIII A'CryG_T

Cuts at:      0      74    1095 
   Size:         74    1021
 
AhdI GACnn_n'nnGTC

Cuts at:      0     135     618    1095 
   Size:        135     483     477

  Fragments arranged by size: 

                 483     477     135
 
AlwNI CAG_nnn'CTG

Cuts at:      0     387     705     747     783    1095 
   Size:        387     318      42      36     312

  Fragments arranged by size: 

                 387     318     312      42      36
 
ApaLI G'TGCA_C

Cuts at:      0     439    1095 
   Size:        439     656
 
AvaI C'yCGr_G

Cuts at:      0     571    1095 
   Size:        571     524
 
BanI G'GyrC_C

Cuts at:      0     560     720    1073    1095 
   Size:        560     160     353      22

  Fragments arranged by size: 

                 560     353     160      22
 
BanII G_rGCy'C

Cuts at:      0     260     570    1095 
   Size:        260     310     525

  Fragments arranged by size: 

                 525     310     260
 
BbeI G_GCGC'C

Cuts at:      0    1077    1095 
   Size:       1077      18
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     261    1095 
   Size:        261     834
 
BbvCI CC'TCA_GC

Cuts at:      0     583    1095 
   Size:        583     512
 
BglI GCCn_nnn'nGGC

Cuts at:      0     901    1082    1095 
   Size:        901     181      13

  Fragments arranged by size: 

                 901     181      13
 
BglII A'GATC_T

Cuts at:      0     188    1095 
   Size:        188     907
 
BlpI GC'TnA_GC

Cuts at:      0     166    1095 
   Size:        166     929
 
Bme1580I G_kGCm'C

Cuts at:      0     443    1095 
   Size:        443     652
 
BmgBI CAC'GTC

Cuts at:      0      90     876    1095 
   Size:         90     786     219

  Fragments arranged by size: 

                 786     219      90
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     874     886    1095 
   Size:        874      12     209

  Fragments arranged by size: 

                 874     209      12
 
Bpu10I CC'TnA_GC

Cuts at:      0     583    1095 
   Size:        583     512
 
BsaAI yAC'GTr

Cuts at:      0      75     546    1095 
   Size:         75     471     549

  Fragments arranged by size: 

                 549     471      75
 
BsaHI Gr'CG_yC

Cuts at:      0      38     527    1074    1095 
   Size:         38     489     547      21

  Fragments arranged by size: 

                 547     489      38      21
 
BsaWI w'CCGG_w

Cuts at:      0      11    1095 
   Size:         11    1084
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     273     385    1095 
   Size:        273     112     710

  Fragments arranged by size: 

                 710     273     112
 
BseYI C'CCAG_C

Cuts at:      0     276     430     959    1095 
   Size:        276     154     529     136

  Fragments arranged by size: 

                 529     276     154     136
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     760    1095 
   Size:        760     335
 
BsiEI CG_ry'CG

Cuts at:      0     896    1095 
   Size:        896     199
 
BsiHKAI G_wGCw'C

Cuts at:      0     121     443    1095 
   Size:        121     322     652

  Fragments arranged by size: 

                 652     322     121
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     943    1095 
   Size:        943     152
 
Bsp1286I G_dGCh'C

Cuts at:      0     121     260     443     570    1095 
   Size:        121     139     183     127     525

  Fragments arranged by size: 

                 525     183     139     127     121
 
BspEI T'CCGG_A

Cuts at:      0      11    1095 
   Size:         11    1084
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     857    1095 
   Size:        857     238
 
BsrDI GCAATG_nn'

Cuts at:      0    1052    1095 
   Size:       1052      43
 
BssHII G'CGCG_C

Cuts at:      0     201    1095 
   Size:        201     894
 
BssSI C'ACGA_G

Cuts at:      0     988    1095 
   Size:        988     107
 
BstEII G'GTnAC_C

Cuts at:      0     549    1095 
   Size:        549     546
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     430    1095 
   Size:        430     665
 
BstYI r'GATC_y

Cuts at:      0     188     336     377     701    1095 
   Size:        188     148      41     324     394

  Fragments arranged by size: 

                 394     324     188     148      41
 
Bsu36I CC'TnA_GG

Cuts at:      0     941    1095 
   Size:        941     154
 
BtgI C'CryG_G

Cuts at:      0     823    1095 
   Size:        823     272
 
DrdI GACnn_nn'nnGTC

Cuts at:      0      75    1095 
   Size:         75    1020
 
EaeI y'GGCC_r

Cuts at:      0     122     152     761     893    1095 
   Size:        122      30     609     132     202

  Fragments arranged by size: 

                 609     202     132     122      30
 
EagI C'GGCC_G

Cuts at:      0     893    1095 
   Size:        893     202
 
EarI CTCTTCn'nnn_

Cuts at:      0     941    1095 
   Size:        941     154
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     247     472     511     874     886    1029    1095 
   Size:        247     225      39     363      12     143      66

  Fragments arranged by size: 

                 363     247     225     143      66      39      12
 
EcoO109I rG'GnC_Cy

Cuts at:      0     101     447     903     936    1095 
   Size:        101     346     456      33     159

  Fragments arranged by size: 

                 456     346     159     101      33
 
FspI TGC'GCA

Cuts at:      0     292    1095 
   Size:        292     803
 
HaeII r_GCGC'y

Cuts at:      0     888    1077    1095 
   Size:        888     189      18

  Fragments arranged by size: 

                 888     189      18
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     121     153     604     636    1095 
   Size:        121      32     451      32     459

  Fragments arranged by size: 

                 459     451     121      32      32
 
HincII GTy'rAC

Cuts at:      0      42    1095 
   Size:         42    1053
 
KasI G'GCGC_C

Cuts at:      0    1073    1095 
   Size:       1073      22
 
KpnI G_GTAC'C

Cuts at:      0     564    1095 
   Size:        564     531
 
MscI TGG'CCA

Cuts at:      0     154    1095 
   Size:        154     941
 
MslI CAynn'nnrTG

Cuts at:      0     355    1095 
   Size:        355     740
 
MspA1I CmG'CkG

Cuts at:      0      98     557     587     693     816    1095 
   Size:         98     459      30     106     123     279

  Fragments arranged by size: 

                 459     279     123     106      98      30
 
NarI GG'CG_CC

Cuts at:      0    1074    1095 
   Size:       1074      21
 
NspI r_CATG'y

Cuts at:      0     237     523    1095 
   Size:        237     286     572

  Fragments arranged by size: 

                 572     286     237
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     387     609    1095 
   Size:        387     222     486

  Fragments arranged by size: 

                 486     387     222
 
PfoI T'CCnGG_A

Cuts at:      0     260     932    1095 
   Size:        260     672     163

  Fragments arranged by size: 

                 672     260     163
 
PpuMI rG'GwC_Cy

Cuts at:      0     447     936    1095 
   Size:        447     489     159

  Fragments arranged by size: 

                 489     447     159
 
PshAI GACnn'nnGTC

Cuts at:      0      88    1095 
   Size:         88    1007
 
PstI C_TGCA'G

Cuts at:      0     344    1095 
   Size:        344     751
 
PvuII CAG'CTG

Cuts at:      0     587     693     816    1095 
   Size:        587     106     123     279

  Fragments arranged by size: 

                 587     279     123     106
 
SanDI GG'GwC_CC

Cuts at:      0     936    1095 
   Size:        936     159
 
SexAI A'CCwGG_T

Cuts at:      0     311     386    1095 
   Size:        311      75     709

  Fragments arranged by size: 

                 709     311      75
 
SfcI C'TryA_G

Cuts at:      0     340    1095 
   Size:        340     755
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0     901    1095 
   Size:        901     194
 
SfoI GGC'GCC

Cuts at:      0    1075    1095 
   Size:       1075      20
 
SmlI C'TyrA_G

Cuts at:      0     412    1095 
   Size:        412     683
 
StuI AGG'CCT

Cuts at:      0     410    1095 
   Size:        410     685
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     925    1095 
   Size:        925     170
 
TatI w'GTAC_w

Cuts at:      0     416    1095 
   Size:        416     679
 
XmnI GAAnn'nnTTC

Cuts at:      0     805    1095 
   Size:        805     290
 
ZraI GAC'GTC

Cuts at:      0      39    1095 
   Size:         39    1056
 

 Enzymes that do cut:

    AatII   Acc65I     AcuI    AflII   AflIII     AhdI    AlwNI    ApaLI
     AvaI     BanI    BanII     BbeI     BbsI    BbvCI     BglI    BglII
     BlpI Bme1580I    BmgBI     BpmI   Bpu10I    BsaAI    BsaHI    BsaWI
    BseRI    BseYI     BsgI    BsiEI  BsiHKAI    BsmBI Bsp1286I    BspEI
    BspMI    BsrDI   BssHII    BssSI   BstEII    BstXI    BstYI   Bsu36I
     BtgI     DrdI     EaeI     EagI     EarI  Eco57MI EcoO109I     FspI
    HaeII    Hin4I   HincII     KasI     KpnI     MscI     MslI   MspA1I
     NarI     NspI    PflMI     PfoI    PpuMI    PshAI     PstI    PvuII
    SanDI    SexAI     SfcI     SfiI     SfoI     SmlI     StuI    TaqII
     TatI     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AccI     AclI     AfeI     AgeI     AleI     AlfI     AloI
     ApaI     ApoI     AscI     AseI    AsiSI    AvrII     BaeI    BamHI
     BcgI    BciVI     BclI    BfrBI     BmrI     BmtI     BplI    BpuEI
     BsaI    BsaBI    BsaXI    BsiWI     BsmI    BspHI    BsrBI    BsrFI
    BsrGI   BstAPI    BstBI  BstZ17I    BtgZI     BtsI     ClaI    CspCI
     DraI   DraIII     EciI  EcoICRI    EcoNI    EcoRI    EcoRV     FalI
     FseI    FspAI  HindIII     HpaI     MfeI     MluI     MmeI     NaeI
     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     PacI
     PasI     PciI     PmeI     PmlI     PpiI     PsiI   PspOMI    PspXI
     PsrI     PvuI    RsrII     SacI    SacII     SalI     SapI     SbfI
     ScaI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI
     SspI     StyI     SwaI     TstI  Tth111I     XbaI     XcmI     XhoI
     XmaI
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