Restriction map for pF1KA1878
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# NO CUT ENZYMES (  127 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AflII       AflIII      AgeI        AhdI        Alw44I      
AocI        ApaLI       AseI        AsnI        Asp718I     
AspEI       AspI        AviII       AvrII       BbrPI       
BbsI        BciVI       BclI        BfrI        BlnI        
BlpI        BpmI        Bpu1102I    BpuAI       BsaAI       
BsaBI       BsaWI       BseAI       BsiWI       BsmI        
Bsp1407I    BspDI       BspEI       BspLU11I    BspMI       
BsrDI       BsrGI       Bst1107I    BstAPI      BstBI       
BstEII      BstPI       BstXI       BstZ17I     Bsu36I      
CelII       ClaI        CpoI        DraI        DraIII      
Eam1105I    EarI        Eco81I      EcoNI       EcoO65I     
EcoRV       EcoT14I     EcoT22I     FbaI        FseI        
FspI        HincII      HindII      HindIII     HpaI        
KpnI        Ksp632I     MaeII       MaeIII      MamI        
MfeI        MluI        MroI        MseI        MunI        
NcoI        NdeI        NheI        NotI        NruI        
NsiI        NspI        NspV        PacI        PaeR7I      
PflFI       PinAI       PmaCI       PmeI        PmlI        
PpuMI       PshAI       PshBI       Psp1406I    PstI        
PvuI        RsrII       SacI        SalI        SapI        
SbfI        SexAI       SfiI        SfuI        SgrAI       
SmlI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        StuI        StyI        SwaI        
TfiI        Tru9I       Tsp45I      Tth111I     XbaI        
XcmI        XhoI        


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# ONE CUT ENZYMES (   31 enzymes) #
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AcsI          910  GAATTC               [GA]^AATT[CT]
AlwNI         254  CAGCGCCTG            CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI       605  AGCGCT               AGC^GCT
ApoI          910  GAATTC               [GA]^AATT[CT]
AscI          833  GGCGCGCC             GG^CGCGCC
BalI          610  TGGCCA               TGG^CCA
BamHI         518  GGATCC               G^GATCC
BcgI          222  CGAGTTGGATGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI         1130  CTAG                 C^TAG
BglII         536  AGATCT               A^GATCT
BseRI         871  GAGGAG               GAGGAG(10/8)
BsmBI         202  GAGACG               CGTCTC(1/5)
BsmFI         506  GGGAC                GGGAC(10/14)
BspHI         993  TCATGA               T^CATGA
DrdI         1051  GACAATAAGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III      605  AGCGCT               AGC^GCT
EcoRI         910  GAATTC               G^AATTC
HinfI         352  GACTC                G^A[ACGT]TC
MaeI         1130  CTAG                 C^TAG
MboII        1073  GAAGA                GAAGA(8/7)
MluNI         610  TGGCCA               TGG^CCA
MscI          610  TGGCCA               TGG^CCA
MslI           69  CATCGCTGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
PflMI         253  CCAGCGCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI          352  GACTC                GAGTC(4/5)
RcaI          993  TCATGA               T^CATGA
ScaI           95  AGTACT               AGT^ACT
TaqI          449  TCGA                 T^CGA
Tsp509I       911  AATT                 ^AATT
TthHB8I       449  TCGA                 T^CGA
Van91I        253  CCAGCGCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG


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# TWO CUT ENZYMES (   16 enzymes) #
###################################

AlwI          519  GATCC                GGATC(4/5)
              518  GGATC               
Asp700I       471  GAAGGACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              906  GAATGAATTC          
AspHI         112  GAGCAC               G[AT]GC[AT]^C
             1010  GTGCTC              
AvaII         507  GGACC                G^G[AT]CC
              801  GGTCC               
BsaI          457  GAGACC               GGTCTC(1/5)
              997  GAGACC              
BsiHKAI       112  GAGCAC               G[AT]GC[AT]^C
             1010  GTGCTC              
BsrBI         303  GAGCGG               CCGCTC(-3/-3)
              662  GAGCGG              
BstF5I        228  GGATG                GGATG(2/0)
              737  GGATG               
BstYI         518  GGATCC               [GA]^GATC[CT]
              536  AGATCT              
FokI          228  GGATG                GGATG(9/13)
              737  GGATG               
HphI          357  GGTGA                GGTGA(8/7)
               89  TCACC               
MflI          518  GGATCC               [GA]^GATC[CT]
              536  AGATCT              
NlaIII        920  CATG                 CATG^
              994  CATG                
PvuII         405  CAGCTG               CAG^CTG
             1046  CAGCTG              
XhoII         518  GGATCC               [GA]^GATC[CT]
              536  AGATCT              
XmnI          471  GAAGGACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              906  GAATGAATTC          


#########################################
# 3 OR MORE CUT ENZYMES (   84 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AluI        
ApaI        AvaI        BanI        BanII       BbeI        
BbvCI       BbvI        BcnI        BglI        BmyI        
BsaHI       BsaJI       BsgI        BsiEI       BsiYI       
BslI        BsmAI       BsoBI       Bsp1286I    BsrFI       
BsrI        BssHII      BssKI       BssSI       BstNI       
BstUI       Cac8I       CfoI        Cfr10I      Cfr13I      
DdeI        DpnII       DraII       DsaI        EaeI        
EagI        EclXI       Eco52I      EcoO109I    EcoRII      
FauI        Fnu4HI      HaeII       HaeIII      HapII       
HgaI        HhaI        Hin1I       HinP1I      HpaII       
ItaI        KasI        KspI        MboI        MnlI        
MspA1I      MspI        MvaI        MvnI        MwoI        
NaeI        NarI        NciI        NdeII       NgoMIV      
NlaIV       RsaI        SacII       Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        SfoI        SmaI        
TseI        TspRI       XmaCI       XmaI        
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