Restriction map for pF1KE0452
##################################
# NO CUT ENZYMES (  133 enzymes) #
##################################

AatII       AccI        AccII       AccIII      AclI        
AcyI        AflII       AgeI        AhdI        Alw44I      
AlwNI       Aor51HI     ApaLI       AscI        AseI        
AsnI        Asp700I     AspEI       AspI        AviII       
AvrII       BalI        BbeI        BbrPI       BbvCI       
BcnI        BfrI        BglI        BglII       BlnI        
BsaAI       BsaBI       BsaHI       BsaWI       BseAI       
BseRI       BsgI        BsiEI       BsiWI       BsmBI       
BsmI        BspDI       BspEI       BspHI       BspMI       
BsrBI       BsrFI       BssHII      BssSI       Bst1107I    
BstBI       BstEII      BstPI       BstUI       BstZ17I     
Cfr10I      ClaI        CpoI        DraIII      DrdI        
EaeI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      EcoO65I     EcoRI       EcoRV       FauI        
FseI        FspI        HaeII       HgaI        Hin1I       
HindIII     KasI        KspI        MaeII       MamI        
MluI        MluNI       MroI        MscI        MvnI        
NaeI        NarI        NciI        NdeI        NgoMIV      
NotI        NruI        NspV        PacI        PaeR7I      
PflFI       PflMI       PinAI       PmaCI       PmeI        
PmlI        PshAI       PshBI       Psp1406I    PstI        
PvuI        RcaI        RsrII       SacII       SalI        
SbfI        ScaI        SfcI        SfiI        SfoI        
SfuI        SgrAI       SmaI        SmlI        SnaBI       
SpeI        SphI        Sse8387I    StuI        SwaI        
Tth111I     Van91I      XbaI        XcmI        XhoI        
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   69 enzymes) #
###################################

AciI         1163  CCGC                 CCGC(-3/-1)
AocI          851  CCTCAGG              CC^T[ACGT]AGG
ApaI         1068  GGGCCC               GGGCC^C
AspHI         110  GAGCTC               G[AT]GC[AT]^C
AvaI          488  CTCGGG               C^[CT]CG[GA]G
AvaII         393  GGACC                G^G[AT]CC
BamHI         492  GGATCC               G^GATCC
BbsI          768  GAAGAC               GAAGAC(2/6)
BbvI         1374  GCTGC                GCAGC(8/12)
BcgI         1110  GCAAAACACTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI        1075  GGATAC               GTATCC(6/5)
BclI          690  TGATCA               T^GATCA
BlpI          530  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I      530  GCTCAGC              GC^T[ACGT]AGC
BpuAI         768  GAAGAC               GAAGAC(2/6)
BsiHKAI       110  GAGCTC               G[AT]GC[AT]^C
BsmFI         392  GGGAC                GGGAC(10/14)
BsoBI         488  CTCGGG               C^[CT]CG[GA]G
Bsp1407I      701  TGTACA               T^GTACA
BsrDI         776  GCAATG               GCAATG(2/0)
BsrGI         701  TGTACA               T^GTACA
BstAPI       1351  GCAACTTATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI         617  CCACTATTTTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI         492  GGATCC               [GA]^GATC[CT]
Bsu36I        851  CCTCAGG              CC^T[ACGT]AGG
CelII         530  GCTCAGC              GC^T[ACGT]AGC
CfoI          528  GCGC                 GCG^C
DraII         392  GGGACCC              [GA]G^G[ACGT]CC[CT]
DsaI         1099  CCATGG               C^C[GA][CT]GG
EarI          782  CTCTTC               CTCTTC(1/4)
Eco81I        851  CCTCAGG              CC^T[ACGT]AGG
EcoNI         749  CCTTTTACAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I      392  GGGACCC              [GA]G^G[ACGT]CC[CT]
EcoT14I      1099  CCATGG               C^C[AT][AT]GG
EcoT22I      1358  ATGCAT               ATGCA^T
FbaI          690  TGATCA               T^GATCA
HapII         745  CCGG                 C^CGG
HhaI          528  GCGC                 GCG^C
HinP1I        528  GCGC                 G^CGC
HincII        843  GTTAAC               GT[CT]^[GA]AC
HindII        843  GTTAAC               GT[CT]^[GA]AC
HpaI          843  GTTAAC               GTT^AAC
HpaII         745  CCGG                 C^CGG
HphI         1196  TCACC                GGTGA(8/7)
Ksp632I       782  CTCTTC               CTCTTC(1/4)
MfeI          539  CAATTG               C^AATTG
MflI          492  GGATCC               [GA]^GATC[CT]
MslI          922  CATAAGTATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I       1372  CAGCTG               C[AC]G^C[GT]G
MspI          745  CCGG                 C^CGG
MunI          539  CAATTG               C^AATTG
MwoI         1351  GCAACTTATGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NcoI         1099  CCATGG               C^CATGG
NheI         1015  GCTAGC               G^CTAGC
NsiI         1358  ATGCAT               ATGCA^T
PleI         1314  GACTC                GAGTC(4/5)
PpuMI         392  GGGACCC              [GA]G^G[AT]CC[CT]
PvuII        1372  CAGCTG               CAG^CTG
SacI          110  GAGCTC               GAGCT^C
SapI          781  GCTCTTC              GCTCTTC(1/4)
SexAI        1293  ACCAGGT              A^CC[AT]GGT
SspBI         701  TGTACA               T^GTACA
SspI         1050  AATATT               AAT^ATT
StyI         1099  CCATGG               C^C[AT][AT]GG
TaqI          684  TCGA                 T^CGA
TseI         1374  GCTGC                G^C[AT]GC
Tsp45I        191  GTGAC                ^GT[GC]AC
TthHB8I       684  TCGA                 T^CGA
XhoII         492  GGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   24 enzymes) #
###################################

Acc65I        563  GGTACC               G^GTACC
              612  GGTACC              
AflIII        309  ACATGT               A^C[GA][CT]GT
              698  ACATGT              
AlwI          493  GATCC                GGATC(4/5)
              492  GGATC               
Asp718I       563  GGTACC               G^GTACC
              612  GGTACC              
BanI          563  GGTACC               G^G[CT][GA]CC
              612  GGTACC              
BanII         110  GAGCTC               G[GA]GC[CT]^C
             1068  GGGCCC              
BmyI          110  GAGCTC               G[AGT]GC[ACT]^C
             1068  GGGCCC              
BpmI          437  CTCCAG               CTGGAG(16/14)
              453  CTCCAG              
BsaI           87  GAGACC               GGTCTC(1/5)
              857  GGTCTC              
BsaJI        1071  CCCAGG               C^C[ACGT][ACGT]GG
             1099  CCATGG              
Bsp1286I      110  GAGCTC               G[AGT]GC[ACT]^C
             1068  GGGCCC              
BspLU11I      309  ACATGT               A^CATGT
              698  ACATGT              
BssKI        1072  CCAGG                ^CC[ACGT]GG
             1294  CCAGG               
BstNI        1072  CCAGG                CC^[AT]GG
             1294  CCAGG               
Cac8I        1015  GCTAGC               GC[ACGT]^[ACGT]GC
             1370  GCCAGC              
DraI           75  TTTAAA               TTT^AAA
              225  TTTAAA              
EcoRII       1072  CCAGG                ^CC[AT]GG
             1294  CCAGG               
Fnu4HI       1162  GCCGC                GC^[ACGT]GC
             1374  GCTGC               
ItaI         1162  GCCGC                GC^[ACGT]GC
             1374  GCTGC               
KpnI          563  GGTACC               GGTAC^C
              612  GGTACC              
MvaI         1072  CCAGG                CC^[AT]GG
             1294  CCAGG               
NspI          309  ACATGT               [GA]CATG^[CT]
              698  ACATGT              
ScrFI        1072  CCAGG                CC^[ACGT]GG
             1294  CCAGG               
SfaNI          26  GCATC                GCATC(5/9)
             1091  GCATC               


#########################################
# 3 OR MORE CUT ENZYMES (   32 enzymes) #
#########################################

AcsI        AfaI        AluI        ApoI        BfaI        
BsiYI       BslI        BsmAI       BsrI        BstF5I      
Cfr13I      DdeI        DpnII       FokI        HaeIII      
HinfI       MaeI        MaeIII      MboI        MboII       
MnlI        MseI        NdeII       NlaIII      NlaIV       
RsaI        Sau3AI      Sau96I      TfiI        Tru9I       
Tsp509I     TspRI       
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