Restriction map for pF1KB6731
##################################
# NO CUT ENZYMES (  199 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AclI        
AcsI        AcyI        AfaI        AflII       AgeI        
AhdI        Alw44I      AocI        Aor51HI     ApaI        
ApaLI       ApoI        AscI        AseI        AsnI        
Asp718I     AspEI       AspHI       AspI        AvaI        
AviII       AvrII       BalI        BamHI       BanI        
BbeI        BbrPI       BbsI        BbvCI       BcgI        
BciVI       BclI        BcnI        BfaI        BfrI        
BglI        BglII       BlnI        BlpI        BpmI        
Bpu1102I    BpuAI       BsaHI       BsaI        BsaJI       
BsaWI       BseAI       BseRI       BsiEI       BsiHKAI     
BsiWI       BsmAI       BsmBI       BsmI        BsoBI       
Bsp1407I    BspDI       BspEI       BspHI       BspMI       
BsrBI       BsrDI       BsrFI       BsrGI       BsrI        
BssHII      BssKI       BssSI       Bst1107I    BstAPI      
BstBI       BstEII      BstNI       BstPI       BstUI       
BstXI       BstYI       BstZ17I     Bsu36I      CelII       
CfoI        Cfr10I      ClaI        CpoI        DraI        
DraII       DraIII      DsaI        EaeI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      Eco81I      
EcoNI       EcoO109I    EcoO65I     EcoRI       EcoRII      
EcoRV       EcoT14I     EcoT22I     FauI        FbaI        
FseI        FspI        HaeII       HaeIII      HapII       
HgaI        HhaI        Hin1I       HinP1I      HindIII     
HinfI       HpaI        HpaII       HphI        KasI        
KpnI        KspI        MaeI        MfeI        MflI        
MluI        MluNI       MroI        MscI        MseI        
MslI        MspI        MunI        MvaI        MvnI        
MwoI        NaeI        NarI        NciI        NcoI        
NdeI        NgoMIV      NheI        NotI        NruI        
NsiI        NspV        PacI        PaeR7I      PflFI       
PinAI       PleI        PmaCI       PmeI        PmlI        
PpuMI       PshAI       PshBI       Psp1406I    PvuI        
RcaI        RsaI        RsrII       SacI        SacII       
SbfI        ScaI        ScrFI       SexAI       SfiI        
SfoI        SfuI        SgrAI       SmaI        SmlI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SspI        StuI        StyI        SwaI        TfiI        
Tru9I       Tsp509I     Tth111I     XbaI        XcmI        
XhoI        XhoII       XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   47 enzymes) #
###################################

AccI          229  GTCGAC               GT^[AC][GT]AC
AciI          253  GCGG                 CCGC(-3/-1)
AflIII        158  ACATGT               A^C[GA][CT]GT
AluI          212  AGCT                 AG^CT
AlwI           96  GGATC                GGATC(4/5)
AlwNI         137  CAGACCCTG            CAG[ACGT][ACGT][ACGT]^CTG
Asp700I       174  GAACTGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaII         198  GGTCC                G^G[AT]CC
BanII         189  GGGCTC               G[GA]GC[CT]^C
BmyI          189  GGGCTC               G[AGT]GC[ACT]^C
BsaAI         205  TACGTG               [CT]AC^GT[GA]
BsaBI          97  GATCAGTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsgI          208  GTGCAG               GTGCAG(16/14)
BsiYI          87  CCATTCTGTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI           87  CCATTCTGTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmFI          24  GTCCC                GGGAC(10/14)
Bsp1286I      189  GGGCTC               G[AGT]GC[ACT]^C
BspLU11I      158  ACATGT               A^CATGT
Cac8I         216  GCCAGC               GC[ACGT]^[ACGT]GC
Cfr13I        198  GGTCC                G^G[ACGT]CC
DpnII          97  GATC                 ^GATC
DrdI          124  GACATCAGTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI            4  CTCTTC               CTCTTC(1/4)
HincII        229  GTCGAC               GT[CT]^[GA]AC
HindII        229  GTCGAC               GT[CT]^[GA]AC
Ksp632I         4  CTCTTC               CTCTTC(1/4)
MaeII         206  ACGT                 A^CGT
MaeIII        133  GTCAC                ^GT[ACGT]AC
MamI           97  GATCAGTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MboI           97  GATC                 ^GATC
MspA1I        211  CAGCTG               C[AC]G^C[GT]G
NdeII          97  GATC                 ^GATC
NlaIII        159  CATG                 CATG^
NlaIV          79  GGAACC               GG[ACGT]^[ACGT]CC
NspI          158  ACATGT               [GA]CATG^[CT]
PflMI          87  CCATTCTGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI          271  CTGCAG               CTGCA^G
PvuII         211  CAGCTG               CAG^CTG
SalI          229  GTCGAC               G^TCGAC
SapI            3  GCTCTTC              GCTCTTC(1/4)
Sau3AI         97  GATC                 ^GATC
Sau96I        198  GGTCC                G^G[ACGT]CC
TaqI          230  TCGA                 T^CGA
Tsp45I        133  GTCAC                ^GT[GC]AC
TthHB8I       230  TCGA                 T^CGA
Van91I         87  CCATTCTGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI          174  GAACTGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (    5 enzymes) #
###################################

BstF5I         39  GGATG                GGATG(2/0)
              249  GGATG               
FokI           39  GGATG                GGATG(9/13)
              249  GGATG               
MboII         171  GAAGA                GAAGA(8/7)
                5  TCTTC               
SfaNI         250  GATGC                GCATC(5/9)
               71  GCATC               
SfcI          244  CTACAG               C^T[GA][CT]AG
              271  CTGCAG              


#########################################
# 3 OR MORE CUT ENZYMES (    7 enzymes) #
#########################################

BbvI        DdeI        Fnu4HI      ItaI        MnlI        
TseI        TspRI       
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