Restriction map for pF1KB6731
 (Circular) (Six-base) MAPSORT of: pF1KB6731.seq  Check: 8431  from: 1  to: 3398



pF1KB6731  3398 bp

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:48  ..

AccI GT'mk_AC

Cuts at:    338     436     338 
   Size:         98    3300
 
Acc65I G'GTAC_C

Cuts at:    414     414 
   Size:       3398
 
AclI AA'CG_TT

Cuts at:   3238    3238 
   Size:       3398
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1223    1655    2402    2670    1223 
   Size:        432     747     268    1951

  Fragments arranged by size: 

                1951     747     432     268
 
AflIII A'CryG_T

Cuts at:    266     750    1854    2831     266 
   Size:        484    1104     977     833

  Fragments arranged by size: 

                1104     977     833     484
 
AgeI A'CCGG_T

Cuts at:   1786    1786 
   Size:       3398
 
AlwNI CAG_nnn'CTG

Cuts at:    250     703    2270     250 
   Size:        453    1567    1378

  Fragments arranged by size: 

                1567    1378     453
 
ApaLI G'TGCA_C

Cuts at:   2168    2168 
   Size:       3398
 
ApoI r'AATT_y

Cuts at:    402    2751    3007     402 
   Size:       2349     256     793

  Fragments arranged by size: 

                2349     793     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       3398
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3398
 
AvaI C'yCGr_G

Cuts at:    418     418 
   Size:       3398
 
BamHI G'GATC_C

Cuts at:    423     423 
   Size:       3398
 
BanI G'GyrC_C

Cuts at:    414     828    1076    1111     414 
   Size:        414     248      35    2701

  Fragments arranged by size: 

                2701     414     248      35
 
BanII G_rGCy'C

Cuts at:    301     412    1442     301 
   Size:        111    1030    2257

  Fragments arranged by size: 

                2257    1030     111
 
BbeI G_GCGC'C

Cuts at:    832    1080     832 
   Size:        248    3150
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:     90     124      90 
   Size:         34    3364
 
BciVI GTATCCnnnnn_n'

Cuts at:   1296    2057    3398    1296 
   Size:        761    1341    1296

  Fragments arranged by size: 

                1341    1296     761
 
BclI T'GATC_A

Cuts at:    918     918 
   Size:       3398
 
BglII A'GATC_T

Cuts at:    913    2950     913 
   Size:       2037    1361
 
BlpI GC'TnA_GC

Cuts at:    510     510 
   Size:       3398
 
Bme1580I G_kGCm'C

Cuts at:   1023    1116    2172    1023 
   Size:         93    1056    2249

  Fragments arranged by size: 

                2249    1056      93
 
BmrI ACTGGGnnnn_n'

Cuts at:    610     691    1026    3170     610 
   Size:         81     335    2144     838

  Fragments arranged by size: 

                2144     838     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   1765    1765 
   Size:       3398
 
Bpu10I CC'TnA_GC

Cuts at:    175     175 
   Size:       3398
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    574    1453    1945    2243     574 
   Size:        879     492     298    1729

  Fragments arranged by size: 

                1729     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   3102    3102 
   Size:       3398
 
BsaAI yAC'GTr

Cuts at:    315     751     315 
   Size:        436    2962
 
BsaBI GATnn'nnATC

Cuts at:      6     209     912    2949       6 
   Size:        203     703    2037     455

  Fragments arranged by size: 

                2037     703     455     203
 
BsaHI Gr'CG_yC

Cuts at:    829    1077    1779    3275     829 
   Size:        248     702    1496     952

  Fragments arranged by size: 

                1496     952     702     248
 
BsaWI w'CCGG_w

Cuts at:    587    1108    1786    2060    2207    2618     587 
   Size:        521     678     274     147     411    1367

  Fragments arranged by size: 

                1367     678     521     411     274     147
 
BseYI C'CCAG_C

Cuts at:    823    1581    2158    2654     823 
   Size:        758     577     496    1567

  Fragments arranged by size: 

                1567     758     577     496
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:    337     337 
   Size:       3398
 
BsiEI CG_ry'CG

Cuts at:    104     940     986    2194     104 
   Size:        836      46    1208    1308

  Fragments arranged by size: 

                1308    1208     836      46
 
BsiHKAI G_wGCw'C

Cuts at:    412    1190    1380    2172     412 
   Size:        778     190     792    1638

  Fragments arranged by size: 

                1638     792     778     190
 
Bsp1286I G_dGCh'C

Cuts at:    301     412    1023    1116    1190    1380    1442    2172     301 
   Size:        111     611      93      74     190      62     730    1527

  Fragments arranged by size: 

                1527     730     611     190     111      93      74      62
            
 
BspEI T'CCGG_A

Cuts at:   2618    2618 
   Size:       3398
 
BspHI T'CATG_A

Cuts at:   2574    2574 
   Size:       3398
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    448     662     964    1345     448 
   Size:        214     302     381    2501

  Fragments arranged by size: 

                2501     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50    1690    1744    3228    3393      50 
   Size:       1640      54    1484     165      55

  Fragments arranged by size: 

                1640    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   1310    1310 
   Size:       3398
 
BsrFI r'CCGG_y

Cuts at:   1396    1577    1786    1396 
   Size:        181     209    3008

  Fragments arranged by size: 

                3008     209     181
 
BssSI C'ACGA_G

Cuts at:   1669    2027    1669 
   Size:        358    3040
 
BstAPI GCAn_nnn'nTGC

Cuts at:    775     775 
   Size:       3398
 
BstBI TT'CG_AA

Cuts at:   1759    1759 
   Size:       3398
 
BstYI r'GATC_y

Cuts at:      1     423     890     913    1248    1494    2506    2592    2604 
   Size:        422     467      23     335     246    1012      86      12

Cuts at:   2604    2950       1 
   Size:        346     449

  Fragments arranged by size: 

                1012     467     449     422     346     335     246      86
                  23      12
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   1348    1529    1692    1348 
   Size:        181     163    3054

  Fragments arranged by size: 

                3054     181     163
 
BtsI GCAGTG_nn'

Cuts at:    641     641 
   Size:       3398
 
DraI TTT'AAA

Cuts at:    397     397 
   Size:       3398
 
DrdI GACnn_nn'nnGTC

Cuts at:    238    1104    1962     238 
   Size:        866     858    1674

  Fragments arranged by size: 

                1674     866     858
 
EaeI y'GGCC_r

Cuts at:    983    1157    1548    1575    2920     983 
   Size:        174     391      27    1345    1461

  Fragments arranged by size: 

                1461    1345     391     174      27
 
EagI C'GGCC_G

Cuts at:    983     983 
   Size:       3398
 
EarI CTCTTCn'nnn_

Cuts at:    118    1421    1631     118 
   Size:       1303     210    1885

  Fragments arranged by size: 

                1885    1303     210
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   1606    1916    2062    3208    1606 
   Size:        310     146    1146    1796

  Fragments arranged by size: 

                1796    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:    410     410 
   Size:       3398
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1223    1655    2402    2670    1223 
   Size:        432     747     268    1951

  Fragments arranged by size: 

                1951     747     432     268
 
EcoO109I rG'GnC_Cy

Cuts at:    537     537 
   Size:       3398
 
EcoRI G'AATT_C

Cuts at:    402     402 
   Size:       3398
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:    303    1417     303 
   Size:       1114    2284
 
FspI TGC'GCA

Cuts at:   1179    1179 
   Size:       3398
 
HaeII r_GCGC'y

Cuts at:    832    1080    2102    3081     832 
   Size:        248    1022     979    1149

  Fragments arranged by size: 

                1149    1022     979     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1240    1270    1272    1302    1240 
   Size:         30       2      30    3336

  Fragments arranged by size: 

                3336      30      30       2
 
HincII GTy'rAC

Cuts at:    339     437     339 
   Size:         98    3300
 
KasI G'GCGC_C

Cuts at:    828    1076     828 
   Size:        248    3150
 
KpnI G_GTAC'C

Cuts at:    418     418 
   Size:       3398
 
MluI A'CGCG_T

Cuts at:   2831    2831 
   Size:       3398
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   2069    2253    2069 
   Size:        184    3214
 
MscI TGG'CCA

Cuts at:   1159    1159 
   Size:       3398
 
MslI CAynn'nnrTG

Cuts at:   1387    1514    1387 
   Size:        127    3271
 
MspA1I CmG'CkG

Cuts at:    321     509     823    1183    2196    2441     321 
   Size:        188     314     360    1013     245    1278

  Fragments arranged by size: 

                1278    1013     360     314     245     188
 
NaeI GCC'GGC

Cuts at:   1579    1579 
   Size:       3398
 
NarI GG'CG_CC

Cuts at:    829    1077     829 
   Size:        248    3150
 
NgoMIV G'CCGG_C

Cuts at:   1577    1577 
   Size:       3398
 
NspI r_CATG'y

Cuts at:    270     451     951    1858     270 
   Size:        181     500     907    1810

  Fragments arranged by size: 

                1810     907     500     181
 
PciI A'CATG_T

Cuts at:    266    1854     266 
   Size:       1588    1810
 
PflMI CCAn_nnn'nTGG

Cuts at:    201     201 
   Size:       3398
 
PmeI GTTT'AAAC

Cuts at:    397     397 
   Size:       3398
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2537    2569    2537 
   Size:         32    3366
 
PstI C_TGCA'G

Cuts at:    383     445    1130     383 
   Size:         62     685    2651

  Fragments arranged by size: 

                2651     685      62
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       3398
 
PvuII CAG'CTG

Cuts at:    321     823    1183     321 
   Size:        502     360    2536

  Fragments arranged by size: 

                2536     502     360
 
RsrII CG'GwC_CG

Cuts at:   1593    1593 
   Size:       3398
 
SacI G_AGCT'C

Cuts at:    412     412 
   Size:       3398
 
SalI G'TCGA_C

Cuts at:    337     435     337 
   Size:         98    3300
 
SapI GCTCTTCn'nnn_

Cuts at:    118    1421    1631     118 
   Size:       1303     210    1885

  Fragments arranged by size: 

                1885    1303     210
 
SbfI CC_TGCA'GG

Cuts at:    445     445 
   Size:       3398
 
ScaI AGT'ACT

Cuts at:   2915    2915 
   Size:       3398
 
SfcI C'TryA_G

Cuts at:     33     352     379     441    1126    2119    2310      33 
   Size:        319      27      62     685     993     191    1121

  Fragments arranged by size: 

                1121     993     685     319     191      62      27
 
SfoI GGC'GCC

Cuts at:    830    1078     830 
   Size:        248    3150
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3398
 
SmaI CCC'GGG

Cuts at:    420     420 
   Size:       3398
 
SmlI C'TyrA_G

Cuts at:    553    1468    1960    2222     553 
   Size:        915     492     262    1729

  Fragments arranged by size: 

                1729     915     492     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       3398
 
SphI G_CATG'C

Cuts at:    451     951     451 
   Size:        500    2898
 
StyI C'CwwG_G

Cuts at:    532     886     532 
   Size:        354    3044
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    981    1114    1573    1782     981 
   Size:        133     459     209    2597

  Fragments arranged by size: 

                2597     459     209     133
 
TatI w'GTAC_w

Cuts at:   2913    2913 
   Size:       3398
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   2915    2947    2915 
   Size:         32    3366
 
Tth111I GACn'n_nGTC

Cuts at:   1195    1195 
   Size:       3398
 
XbaI T'CTAG_A

Cuts at:    429     429 
   Size:       3398
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:    893     893 
   Size:       3398
 
XmaI C'CCGG_G

Cuts at:    418     418 
   Size:       3398
 
XmnI GAAnn'nnTTC

Cuts at:    286     286 
   Size:       3398
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI   AflIII     AgeI    AlwNI    ApaLI
     ApoI     AseI    AsiSI     AvaI    BamHI     BanI    BanII     BbeI
     BcgI    BciVI     BclI    BglII     BlpI Bme1580I     BmrI     BplI
   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BseYI
     BsgI    BsiEI  BsiHKAI Bsp1286I    BspEI    BspHI    BspMI    BsrBI
    BsrDI    BsrFI    BssSI   BstAPI    BstBI    BstYI    BtgZI     BtsI
     DraI     DrdI     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI
 EcoO109I    EcoRI     FalI     FspI    HaeII    Hin4I   HincII     KasI
     KpnI     MluI     MmeI     MscI     MslI   MspA1I     NaeI     NarI
   NgoMIV     NspI     PciI    PflMI     PmeI     PpiI     PstI     PvuI
    PvuII    RsrII     SacI     SalI     SapI     SbfI     ScaI     SfcI
     SfoI     SgfI     SmaI     SmlI     SpeI     SphI     StyI    TaqII
     TatI     TstI  Tth111I     XbaI     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AfeI    AflII     AhdI     AleI     AlfI     AloI
     ApaI     AscI    AvrII     BaeI     BbsI    BbvCI    BfrBI     BglI
    BmgBI     BmtI     BpmI    BsaXI    BseRI    BsiWI     BsmI    BsmBI
    BsrGI   BssHII   BstEII    BstXI  BstZ17I   Bsu36I     BtgI     ClaI
    CspCI   DraIII    EcoNI    EcoRV     FseI    FspAI  HindIII     HpaI
     MfeI     NcoI     NdeI     NheI     NotI     NruI     NsiI     PacI
     PasI     PfoI     PmlI    PpuMI    PshAI     PsiI   PspOMI    PspXI
     PsrI    SacII    SanDI    SexAI     SfiI    SgrAI    SnaBI     SrfI
     SspI     StuI     SwaI     XhoI     ZraI
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