Restriction map for pF1KE0556
##################################
# NO CUT ENZYMES (  146 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AflII       AflIII      AgeI        
Alw44I      AocI        Aor51HI     ApaLI       AscI        
AseI        AsnI        Asp700I     Asp718I     AspHI       
AspI        AvaI        AvrII       BalI        BamHI       
BanI        BbeI        BbsI        BcgI        BciVI       
BclI        BfrI        BglII       BlnI        BlpI        
BpmI        Bpu1102I    BpuAI       BsaBI       BsaHI       
BsaWI       BseAI       BsiEI       BsiHKAI     BsiWI       
BsmBI       BsmI        BsoBI       BspDI       BspEI       
BspHI       BspLU11I    BspMI       BsrBI       BssHII      
BssSI       Bst1107I    BstBI       BstUI       BstXI       
BstZ17I     Bsu36I      CelII       ClaI        CpoI        
DraI        DraIII      DrdI        EagI        EclXI       
Eco47III    Eco52I      Eco81I      EcoRI       EcoRV       
EcoT22I     FbaI        FseI        HgaI        Hin1I       
HincII      HindII      HindIII     HpaI        KasI        
KpnI        KspI        MamI        MfeI        MluI        
MluNI       MroI        MscI        MunI        MvnI        
NaeI        NarI        NdeI        NgoMIV      NheI        
NotI        NruI        NsiI        NspI        NspV        
PacI        PaeR7I      PflFI       PflMI       PinAI       
PmeI        PshAI       PshBI       Psp1406I    PvuI        
RcaI        RsrII       SacI        SacII       SalI        
SbfI        ScaI        SexAI       SfiI        SfoI        
SfuI        SgrAI       SmaI        SmlI        SnaBI       
SpeI        SphI        Sse8387I    SspI        StuI        
SwaI        TaqI        TfiI        Tth111I     TthHB8I     
Van91I      XbaI        XhoI        XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   48 enzymes) #
###################################

AcsI          601  GAATTT               [GA]^AATT[CT]
AhdI          149  GACCCTCAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI          717  GATCC                GGATC(4/5)
ApoI          601  GAATTT               [GA]^AATT[CT]
AspEI         149  GACCCTCAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII         101  TGCGCA               TGC^GCA
BbrPI         664  CACGTG               CAC^GTG
BfaI          556  CTAG                 C^TAG
BsaAI         664  CACGTG               [CT]AC^GT[GA]
BsaI          361  GGTCTC               GGTCTC(1/5)
Bsp1407I      314  TGTACA               T^GTACA
BsrDI         196  GCAATG               GCAATG(2/0)
BsrFI          43  ACCGGC               [GA]^CCGG[CT]
BsrGI         314  TGTACA               T^GTACA
BstAPI        702  GCATCGGCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII        201  GGTGACC              G^GT[ACGT]ACC
BstPI         201  GGTGACC              G^GT[ACGT]ACC
BstYI         716  AGATCC               [GA]^GATC[CT]
Cfr10I         43  ACCGGC               [GA]^CCGG[CT]
DpnII         717  GATC                 ^GATC
EaeI          369  TGGCCG               [CT]^GGCC[GA]
Eam1105I      149  GACCCTCAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI          271  CTCTTC               CTCTTC(1/4)
EcoNI         387  CCTGGGGCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I       201  GGTGACC              G^GT[ACGT]ACC
FauI          681  GCGGG                CCCGC(4/6)
FspI          101  TGCGCA               TGC^GCA
HphI          201  GGTGA                GGTGA(8/7)
Ksp632I       271  CTCTTC               CTCTTC(1/4)
MaeI          556  CTAG                 C^TAG
MaeII         665  ACGT                 A^CGT
MaeIII        202  GTGAC                ^GT[ACGT]AC
MboI          717  GATC                 ^GATC
MboII         272  TCTTC                GAAGA(8/7)
MflI          716  AGATCC               [GA]^GATC[CT]
MseI          613  TTAA                 T^TAA
MslI          159  CATGCCAGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NdeII         717  GATC                 ^GATC
PmaCI         664  CACGTG               CAC^GTG
PmlI          664  CACGTG               CAC^GTG
SapI          270  GCTCTTC              GCTCTTC(1/4)
Sau3AI        717  GATC                 ^GATC
SspBI         314  TGTACA               T^GTACA
Tru9I         613  TTAA                 T^TAA
Tsp45I        202  GTGAC                ^GT[GC]AC
Tsp509I       602  AATT                 ^AATT
XcmI          347  CCAGGAACAGCCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII         716  AGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   12 enzymes) #
###################################

AfaI           78  GTAC                 GT^AC
              315  GTAC                
BglI          111  GCCCTGGCGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
              214  GCCTCAGCGGC         
BsrI          243  ACTGG                ACTGG(1/-1)
              163  CCAGT               
EcoT14I       227  CCATGG               C^C[AT][AT]GG
              276  CCATGG              
HaeII         574  AGCGCC               [GA]GCGC^[CT]
              741  GGCGCT              
HinfI         450  GACTC                G^A[ACGT]TC
              728  GACTC               
NcoI          227  CCATGG               C^CATGG
              276  CCATGG              
PleI          450  GACTC                GAGTC(4/5)
              728  GACTC               
PpuMI          61  GGGTCCT              [GA]G^G[AT]CC[CT]
              280  GGGACCC             
RsaI           78  GTAC                 GT^AC
              315  GTAC                
StyI          227  CCATGG               C^C[AT][AT]GG
              276  CCATGG              
TspRI          38  CACTG                CA[GC]TG[ACGT][ACGT]^
              164  CAGTG               


#########################################
# 3 OR MORE CUT ENZYMES (   52 enzymes) #
#########################################

AciI        AluI        AlwNI       ApaI        AvaII       
BanII       BbvCI       BbvI        BcnI        BmyI        
BsaJI       BseRI       BsgI        BsiYI       BslI        
BsmAI       BsmFI       Bsp1286I    BssKI       BstF5I      
BstNI       Cac8I       CfoI        Cfr13I      DdeI        
DraII       DsaI        EcoO109I    EcoRII      Fnu4HI      
FokI        HaeIII      HapII       HhaI        HinP1I      
HpaII       ItaI        MnlI        MspA1I      MspI        
MvaI        MwoI        NciI        NlaIII      NlaIV       
PstI        PvuII       Sau96I      ScrFI       SfaNI       
SfcI        TseI        
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com