Restriction map for pF1KE0556
 (Linear) (Six-base) MAPSORT of: KIEE0556.seq  Check: 317  from: 1  to: 753



KIEE0556.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:16  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     145     753 
   Size:        145     608
 
AhdI GACnn_n'nnGTC

Cuts at:      0     154     753 
   Size:        154     599
 
AlwNI CAG_nnn'CTG

Cuts at:      0      39      53     169     753 
   Size:         39      14     116     584

  Fragments arranged by size: 

                 584     116      39      14
 
ApaI G_GGCC'C

Cuts at:      0     192     589     654     753 
   Size:        192     397      65      99

  Fragments arranged by size: 

                 397     192      99      65
 
ApoI r'AATT_y

Cuts at:      0     601     753 
   Size:        601     152
 
BanII G_rGCy'C

Cuts at:      0     113     192     589     654     753 
   Size:        113      79     397      65      99

  Fragments arranged by size: 

                 397     113      99      79      65
 
BbvCI CC'TCA_GC

Cuts at:      0     216     497     568     753 
   Size:        216     281      71     185

  Fragments arranged by size: 

                 281     216     185      71
 
BglI GCCn_nnn'nGGC

Cuts at:      0     117     220     753 
   Size:        117     103     533

  Fragments arranged by size: 

                 533     117     103
 
Bme1580I G_kGCm'C

Cuts at:      0      33     192     589     654     753 
   Size:         33     159     397      65      99

  Fragments arranged by size: 

                 397     159      99      65      33
 
Bpu10I CC'TnA_GC

Cuts at:      0     216     497     568     753 
   Size:        216     281      71     185

  Fragments arranged by size: 

                 281     216     185      71
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     367     753 
   Size:        367     386
 
BsaAI yAC'GTr

Cuts at:      0     666     753 
   Size:        666      87
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     271     475     585     753 
   Size:        271     204     110     168

  Fragments arranged by size: 

                 271     204     168     110
 
BseYI C'CCAG_C

Cuts at:      0      57     291     504     582     747     753 
   Size:         57     234     213      78     165       6

  Fragments arranged by size: 

                 234     213     165      78      57       6
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      66     568     688     753 
   Size:         66     502     120      65

  Fragments arranged by size: 

                 502     120      66      65
 
Bsp1286I G_dGCh'C

Cuts at:      0      33     113     192     589     654     753 
   Size:         33      80      79     397      65      99

  Fragments arranged by size: 

                 397      99      80      79      65      33
 
BsrDI GCAATG_nn'

Cuts at:      0     203     753 
   Size:        203     550
 
BsrFI r'CCGG_y

Cuts at:      0      43     753 
   Size:         43     710
 
BsrGI T'GTAC_A

Cuts at:      0     314     753 
   Size:        314     439
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     708     753 
   Size:        708      45
 
BstEII G'GTnAC_C

Cuts at:      0     201     753 
   Size:        201     552
 
BstYI r'GATC_y

Cuts at:      0     716     753 
   Size:        716      37
 
BtgI C'CryG_G

Cuts at:      0     227     276     323     753 
   Size:        227      49      47     430

  Fragments arranged by size: 

                 430     227      49      47
 
EaeI y'GGCC_r

Cuts at:      0     369     753 
   Size:        369     384
 
EarI CTCTTCn'nnn_

Cuts at:      0     277     753 
   Size:        277     476
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     145     753 
   Size:        145     608
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     391     753 
   Size:        391     362
 
EcoO109I rG'GnC_Cy

Cuts at:      0      62     189     281     300     586     651     753 
   Size:         62     127      92      19     286      65     102

  Fragments arranged by size: 

                 286     127     102      92      65      62      19
 
FspI TGC'GCA

Cuts at:      0     103     753 
   Size:        103     650
 
HaeII r_GCGC'y

Cuts at:      0     578     745     753 
   Size:        578     167       8

  Fragments arranged by size: 

                 578     167       8
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     140     172     753 
   Size:        140      32     581

  Fragments arranged by size: 

                 581     140      32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     425     753 
   Size:        425     328
 
MslI CAynn'nnrTG

Cuts at:      0     163     753 
   Size:        163     590
 
MspA1I CmG'CkG

Cuts at:      0     220     291     477     496     552     567     672     739 
   Size:        220      71     186      19      56      15     105      67

Cuts at:    739     753 
   Size:         14

  Fragments arranged by size: 

                 220     186     105      71      67      56      19      15
                  14
 
NcoI C'CATG_G

Cuts at:      0     227     276     753 
   Size:        227      49     477

  Fragments arranged by size: 

                 477     227      49
 
PasI CC'CwG_GG

Cuts at:      0      25     753 
   Size:         25     728
 
PfoI T'CCnGG_A

Cuts at:      0     719     753 
   Size:        719      34
 
PmlI CAC'GTG

Cuts at:      0     666     753 
   Size:        666      87
 
PpuMI rG'GwC_Cy

Cuts at:      0      62     281     753 
   Size:         62     219     472

  Fragments arranged by size: 

                 472     219      62
 
PspOMI G'GGCC_C

Cuts at:      0     188     585     650     753 
   Size:        188     397      65     103

  Fragments arranged by size: 

                 397     188     103      65
 
PstI C_TGCA'G

Cuts at:      0     290     476     482     753 
   Size:        290     186       6     271

  Fragments arranged by size: 

                 290     271     186       6
 
PvuII CAG'CTG

Cuts at:      0     291     477     496     567     753 
   Size:        291     186      19      71     186

  Fragments arranged by size: 

                 291     186     186      71      19
 
SanDI GG'GwC_CC

Cuts at:      0     281     753 
   Size:        281     472
 
SapI GCTCTTCn'nnn_

Cuts at:      0     277     753 
   Size:        277     476
 
SfcI C'TryA_G

Cuts at:      0     286     472     478     753 
   Size:        286     186       6     275

  Fragments arranged by size: 

                 286     275     186       6
 
StyI C'CwwG_G

Cuts at:      0     227     276     753 
   Size:        227      49     477

  Fragments arranged by size: 

                 477     227      49
 
TatI w'GTAC_w

Cuts at:      0      77     314     753 
   Size:         77     237     439

  Fragments arranged by size: 

                 439     237      77
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     354     753 
   Size:        354     399
 

 Enzymes that do cut:

     AcuI     AhdI    AlwNI     ApaI     ApoI    BanII    BbvCI     BglI
 Bme1580I   Bpu10I     BsaI    BsaAI    BseRI    BseYI     BsgI Bsp1286I
    BsrDI    BsrFI    BsrGI   BstAPI   BstEII    BstYI     BtgI     EaeI
     EarI  Eco57MI    EcoNI EcoO109I     FspI    HaeII    Hin4I     MmeI
     MslI   MspA1I     NcoI     PasI     PfoI     PmlI    PpuMI   PspOMI
     PstI    PvuII    SanDI     SapI     SfcI     StyI     TatI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AleI     AlfI     AloI    ApaLI     AscI     AseI    AsiSI
     AvaI    AvrII     BaeI    BamHI     BanI     BbeI     BbsI     BcgI
    BciVI     BclI    BfrBI    BglII     BlpI    BmgBI     BmrI     BmtI
     BplI     BpmI    BpuEI    BsaBI    BsaHI    BsaWI    BsaXI    BsiEI
  BsiHKAI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BspMI    BsrBI
   BssHII    BssSI    BstBI    BstXI  BstZ17I   Bsu36I    BtgZI     BtsI
     ClaI    CspCI     DraI   DraIII     DrdI     EagI     EciI  EcoICRI
    EcoRI    EcoRV     FalI     FseI    FspAI   HincII  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     MscI     NaeI     NarI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI     PciI
    PflMI     PmeI     PpiI    PshAI     PsiI    PspXI     PsrI     PvuI
    RsrII     SacI    SacII     SalI     SbfI     ScaI    SexAI     SfiI
     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI
     SrfI     SspI     StuI     SwaI    TaqII     TstI  Tth111I     XbaI
     XhoI     XmaI     XmnI     ZraI
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