Restriction map for
pF1KE0368
(Linear) (Six-base) MAPSORT of: KIEE0368.seq Check: 7339 from: 1 to: 1965
KIEE0368.orf
With 182 enzymes: SgfI *
April 24, 2009 13:13 ..
AccI GT'mk_AC
Cuts at: 0 402 560 1965
Size: 402 158 1405
Fragments arranged by size:
1405 402 158
AflII C'TTAA_G
Cuts at: 0 238 1965
Size: 238 1727
AlwNI CAG_nnn'CTG
Cuts at: 0 482 1242 1965
Size: 482 760 723
Fragments arranged by size:
760 723 482
ApoI r'AATT_y
Cuts at: 0 1456 1526 1965
Size: 1456 70 439
Fragments arranged by size:
1456 439 70
AseI AT'TA_AT
Cuts at: 0 1216 1965
Size: 1216 749
AvaI C'yCGr_G
Cuts at: 0 57 69 1850 1884 1965
Size: 57 12 1781 34 81
Fragments arranged by size:
1781 81 57 34 12
BanI G'GyrC_C
Cuts at: 0 194 1965
Size: 194 1771
BanII G_rGCy'C
Cuts at: 0 64 140 1127 1249 1811 1857 1965
Size: 64 76 987 122 562 46 108
Fragments arranged by size:
987 562 122 108 76 64 46
BbsI GAAGACnn'nnnn_
Cuts at: 0 391 1965
Size: 391 1574
BciVI GTATCCnnnnn_n'
Cuts at: 0 1753 1965
Size: 1753 212
BlpI GC'TnA_GC
Cuts at: 0 1243 1965
Size: 1243 722
Bme1580I G_kGCm'C
Cuts at: 0 415 1120 1965
Size: 415 705 845
Fragments arranged by size:
845 705 415
BmgBI CAC'GTC
Cuts at: 0 201 1965
Size: 201 1764
BmrI ACTGGGnnnn_n'
Cuts at: 0 1238 1644 1965
Size: 1238 406 321
Fragments arranged by size:
1238 406 321
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 111 529 1802 1817 1965
Size: 111 418 1273 15 148
Fragments arranged by size:
1273 418 148 111 15
Bpu10I CC'TnA_GC
Cuts at: 0 63 1340 1965
Size: 63 1277 625
Fragments arranged by size:
1277 625 63
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1655 1965
Size: 1655 310
BsaBI GATnn'nnATC
Cuts at: 0 878 1965
Size: 878 1087
BsaWI w'CCGG_w
Cuts at: 0 455 1965
Size: 455 1510
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 144 174 1815 1845 1965
Size: 144 30 1641 30 120
Fragments arranged by size:
1641 144 120 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 59 1658 1965
Size: 59 1599 307
Fragments arranged by size:
1599 307 59
BseYI C'CCAG_C
Cuts at: 0 773 844 1720 1965
Size: 773 71 876 245
Fragments arranged by size:
876 773 245 71
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 468 1965
Size: 468 1497
BsiEI CG_ry'CG
Cuts at: 0 32 1965
Size: 32 1933
BsiHKAI G_wGCw'C
Cuts at: 0 651 1127 1811 1857 1965
Size: 651 476 684 46 108
Fragments arranged by size:
684 651 476 108 46
BsmI GAATG_Cn'
Cuts at: 0 856 1591 1965
Size: 856 735 374
Fragments arranged by size:
856 735 374
BsmBI CGTCTCn'nnnn_
Cuts at: 0 171 1965
Size: 171 1794
Bsp1286I G_dGCh'C
Cuts at: 0 64 140 415 651 1120 1127 1249 1811
Size: 64 76 275 236 469 7 122 562
Cuts at: 1811 1857 1965
Size: 46 108
Fragments arranged by size:
562 469 275 236 122 108 76 64
46 7
BspEI T'CCGG_A
Cuts at: 0 455 1965
Size: 455 1510
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 414 1398 1716 1784 1965
Size: 414 984 318 68 181
Fragments arranged by size:
984 414 318 181 68
BsrBI CCG'CTC
Cuts at: 0 95 1965
Size: 95 1870
BsrDI GCAATG_nn'
Cuts at: 0 556 1965
Size: 556 1409
BsrFI r'CCGG_y
Cuts at: 0 80 191 1965
Size: 80 111 1774
Fragments arranged by size:
1774 111 80
BssSI C'ACGA_G
Cuts at: 0 1037 1965
Size: 1037 928
BstAPI GCAn_nnn'nTGC
Cuts at: 0 742 1965
Size: 742 1223
BstBI TT'CG_AA
Cuts at: 0 963 1965
Size: 963 1002
BstEII G'GTnAC_C
Cuts at: 0 1896 1965
Size: 1896 69
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1753 1793 1965
Size: 1753 40 172
Fragments arranged by size:
1753 172 40
BstYI r'GATC_y
Cuts at: 0 1827 1965
Size: 1827 138
Bsu36I CC'TnA_GG
Cuts at: 0 533 779 1965
Size: 533 246 1186
Fragments arranged by size:
1186 533 246
BtgI C'CryG_G
Cuts at: 0 564 1421 1965
Size: 564 857 544
Fragments arranged by size:
857 564 544
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1177 1965
Size: 1177 788
BtsI GCAGTG_nn'
Cuts at: 0 596 1276 1965
Size: 596 680 689
Fragments arranged by size:
689 680 596
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 233 268 1965
Size: 233 35 1697
Fragments arranged by size:
1697 233 35
DraIII CAC_nnn'GTG
Cuts at: 0 512 1017 1965
Size: 512 505 948
Fragments arranged by size:
948 512 505
DrdI GACnn_nn'nnGTC
Cuts at: 0 217 1965
Size: 217 1748
EaeI y'GGCC_r
Cuts at: 0 29 653 1965
Size: 29 624 1312
Fragments arranged by size:
1312 624 29
EagI C'GGCC_G
Cuts at: 0 29 1965
Size: 29 1936
EarI CTCTTCn'nnn_
Cuts at: 0 308 1965
Size: 308 1657
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 56 1965
Size: 56 1909
EcoICRI GAG'CTC
Cuts at: 0 1125 1809 1855 1965
Size: 1125 684 46 110
Fragments arranged by size:
1125 684 110 46
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 111 529 1802 1817 1965
Size: 111 418 1273 15 148
Fragments arranged by size:
1273 418 148 111 15
EcoNI CCTnn'n_nnAGG
Cuts at: 0 457 1965
Size: 457 1508
EcoO109I rG'GnC_Cy
Cuts at: 0 1728 1965
Size: 1728 237
EcoRI G'AATT_C
Cuts at: 0 1456 1965
Size: 1456 509
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 253 1965
Size: 253 1712
HaeII r_GCGC'y
Cuts at: 0 90 152 1547 1965
Size: 90 62 1395 418
Fragments arranged by size:
1395 418 90 62
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 993 1025 1158 1190 1839 1871 1965
Size: 993 32 133 32 649 32 94
Fragments arranged by size:
993 649 133 94 32 32 32
HincII GTy'rAC
Cuts at: 0 403 1965
Size: 403 1562
HindIII A'AGCT_T
Cuts at: 0 352 1965
Size: 352 1613
MscI TGG'CCA
Cuts at: 0 655 1965
Size: 655 1310
MslI CAynn'nnrTG
Cuts at: 0 1472 1707 1965
Size: 1472 235 258
Fragments arranged by size:
1472 258 235
NaeI GCC'GGC
Cuts at: 0 193 1965
Size: 193 1772
NgoMIV G'CCGG_C
Cuts at: 0 191 1965
Size: 191 1774
PasI CC'CwG_GG
Cuts at: 0 831 1965
Size: 831 1134
PpuMI rG'GwC_Cy
Cuts at: 0 1728 1965
Size: 1728 237
PspXI vC'TCGA_Gb
Cuts at: 0 1884 1965
Size: 1884 81
PstI C_TGCA'G
Cuts at: 0 1283 1395 1965
Size: 1283 112 570
Fragments arranged by size:
1283 570 112
SacI G_AGCT'C
Cuts at: 0 1127 1811 1857 1965
Size: 1127 684 46 108
Fragments arranged by size:
1127 684 108 46
SalI G'TCGA_C
Cuts at: 0 401 1965
Size: 401 1564
ScaI AGT'ACT
Cuts at: 0 1193 1366 1965
Size: 1193 173 599
Fragments arranged by size:
1193 599 173
SfcI C'TryA_G
Cuts at: 0 1279 1391 1965
Size: 1279 112 574
Fragments arranged by size:
1279 574 112
SmaI CCC'GGG
Cuts at: 0 71 1965
Size: 71 1894
SmlI C'TyrA_G
Cuts at: 0 238 1670 1884 1965
Size: 238 1432 214 81
Fragments arranged by size:
1432 238 214 81
SrfI GCCC'GGGC
Cuts at: 0 71 1965
Size: 71 1894
SspI AAT'ATT
Cuts at: 0 1508 1965
Size: 1508 457
StyI C'CwwG_G
Cuts at: 0 170 1965
Size: 170 1795
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 583 1965
Size: 583 1382
TatI w'GTAC_w
Cuts at: 0 1191 1364 1965
Size: 1191 173 601
Fragments arranged by size:
1191 601 173
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 144 176 1965
Size: 144 32 1789
Fragments arranged by size:
1789 144 32
XbaI T'CTAG_A
Cuts at: 0 1080 1965
Size: 1080 885
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 871 1965
Size: 871 1094
XhoI C'TCGA_G
Cuts at: 0 1884 1965
Size: 1884 81
XmaI C'CCGG_G
Cuts at: 0 69 1965
Size: 69 1896
Enzymes that do cut:
AccI AflII AlwNI ApoI AseI AvaI BanI BanII
BbsI BciVI BlpI Bme1580I BmgBI BmrI BpmI Bpu10I
BpuEI BsaBI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI CspCI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
HaeII Hin4I HincII HindIII MscI MslI NaeI NgoMIV
PasI PpuMI PspXI PstI SacI SalI ScaI SfcI
SmaI SmlI SrfI SspI StyI TaqII TatI TstI
XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII Acc65I AclI AcuI AfeI AflIII AgeI
AhdI AleI AlfI AloI ApaI ApaLI AscI AsiSI
AvrII BaeI BamHI BbeI BbvCI BcgI BclI BfrBI
BglI BglII BmtI BplI BsaI BsaAI BsaHI BsiWI
BspHI BsrGI BssHII BstZ17I ClaI DraI EcoRV FseI
FspI FspAI HpaI KasI KpnI MfeI MluI MmeI
MspA1I NarI NcoI NdeI NheI NotI NruI NsiI
NspI PacI PciI PflMI PfoI PmeI PmlI PpiI
PshAI PsiI PspOMI PsrI PvuI PvuII RsrII SacII
SanDI SapI SbfI SexAI SfiI SfoI SgfI SgrAI
SnaBI SpeI SphI StuI SwaI Tth111I XmnI ZraI