Restriction map for pF1KB6527
##################################
# NO CUT ENZYMES (  139 enzymes) #
##################################

Acc65I      AccI        AccIII      AclI        AcsI        
AflII       AflIII      AgeI        AhdI        Alw44I      
AlwNI       AocI        ApaI        ApaLI       ApoI        
AscI        AseI        AsnI        Asp718I     AspEI       
AspI        AvaII       AviII       AvrII       BbsI        
BbvCI       BcgI        BciVI       BclI        BfaI        
BfrI        BglI        BglII       BlnI        BlpI        
Bpu1102I    BpuAI       BsaBI       BsaI        BsaWI       
BseAI       BseRI       BsiEI       BsiWI       BsmBI       
Bsp1407I    BspDI       BspEI       BspHI       BspLU11I    
BsrBI       BsrGI       BssHII      BssSI       Bst1107I    
BstAPI      BstBI       BstEII      BstF5I      BstPI       
BstZ17I     Bsu36I      CelII       ClaI        CpoI        
DdeI        DraI        DraIII      DrdI        EagI        
Eam1105I    EclXI       Eco52I      Eco81I      EcoO65I     
EcoRI       EcoRV       EcoT22I     FbaI        FokI        
FseI        FspI        HindIII     HpaI        KpnI        
KspI        MaeI        MaeIII      MamI        MfeI        
MluI        MroI        MseI        MunI        NcoI        
NdeI        NheI        NotI        NruI        NsiI        
NspI        NspV        PacI        PaeR7I      PflFI       
PflMI       PinAI       PmeI        PpuMI       PshAI       
PshBI       Psp1406I    PvuI        PvuII       RcaI        
RsrII       SacII       SalI        SbfI        SfiI        
SfuI        SgrAI       SmlI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        SwaI        
TfiI        Tru9I       Tsp45I      Tsp509I     Tth111I     
Van91I      XbaI        XcmI        XhoI        


###################################
# ONE CUT ENZYMES (   46 enzymes) #
###################################

AatII         835  GACGTC               GACGT^C
Aor51HI       119  AGCGCT               AGC^GCT
Asp700I       756  GAAGCCATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI          101  TGGCCA               TGG^CCA
BamHI         301  GGATCC               G^GATCC
BbeI           47  GGCGCC               GGCGC^C
BbrPI         724  CACGTG               CAC^GTG
BpmI         1012  CTGGAG               CTGGAG(16/14)
BsaAI         724  CACGTG               [CT]AC^GT[GA]
BsgI          567  CTGCAC               GTGCAG(16/14)
BsmAI         808  GAGAC                GTCTC(1/5)
BsmI          420  GAATGC               GAATGC(1/-1)
BspMI         925  ACCTGC               ACCTGC(4/8)
BsrDI         164  GCAATG               GCAATG(2/0)
BstXI         467  CCAAGGGCCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI         301  GGATCC               [GA]^GATC[CT]
DpnII         302  GATC                 ^GATC
DsaI           77  CCACGG               C^C[GA][CT]GG
Eco47III      119  AGCGCT               AGC^GCT
EcoNI         885  CCTCAACAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HinfI         810  GACTC                G^A[ACGT]TC
KasI           47  GGCGCC               G^GCGCC
MboI          302  GATC                 ^GATC
MflI          301  GGATCC               [GA]^GATC[CT]
MluNI         101  TGGCCA               TGG^CCA
MscI          101  TGGCCA               TGG^CCA
MslI          451  CACAACCGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI           47  GGCGCC               GG^CGCC
NdeII         302  GATC                 ^GATC
PleI          810  GACTC                GAGTC(4/5)
PmaCI         724  CACGTG               CAC^GTG
PmlI          724  CACGTG               CAC^GTG
PstI          849  CTGCAG               CTGCA^G
SacI          619  GAGCTC               GAGCT^C
Sau3AI        302  GATC                 ^GATC
ScaI          257  AGTACT               AGT^ACT
SexAI         503  ACCTGGT              A^CC[AT]GGT
SfaNI         432  GCATC                GCATC(5/9)
SfcI          849  CTGCAG               C^T[GA][CT]AG
SfoI           47  GGCGCC               GGC^GCC
SmaI          360  CCCGGG               CCC^GGG
StuI          893  AGGCCT               AGG^CCT
XhoII         301  GGATCC               [GA]^GATC[CT]
XmaCI         360  CCCGGG               C^CCGGG
XmaI          360  CCCGGG               C^CCGGG
XmnI          756  GAAGCCATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   12 enzymes) #
###################################

AlwI          302  GATCC                GGATC(4/5)
              301  GGATC               
AvaI          223  CCCGAG               C^[CT]CG[GA]G
              360  CCCGGG              
BsmFI         833  GGGAC                GGGAC(10/14)
              655  GTCCC               
BsoBI         223  CCCGAG               C^[CT]CG[GA]G
              360  CCCGGG              
BsrI          353  ACTGG                ACTGG(1/-1)
              204  CCAGT               
EarI          232  CTCTTC               CTCTTC(1/4)
              826  CTCTTC              
HaeII          47  GGCGCC               [GA]GCGC^[CT]
              119  AGCGCT              
HincII        157  GTCAAC               GT[CT]^[GA]AC
              700  GTCAAC              
HindII        157  GTCAAC               GT[CT]^[GA]AC
              700  GTCAAC              
Ksp632I       232  CTCTTC               CTCTTC(1/4)
              826  CTCTTC              
MaeII         725  ACGT                 A^CGT
              836  ACGT                
SapI          231  GCTCTTC              GCTCTTC(1/4)
              825  GCTCTTC             


#########################################
# 3 OR MORE CUT ENZYMES (   61 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AluI        
AspHI       BanI        BanII       BbvI        BcnI        
BmyI        BsaHI       BsaJI       BsiHKAI     BsiYI       
BslI        Bsp1286I    BsrFI       BssKI       BstNI       
BstUI       Cac8I       CfoI        Cfr10I      Cfr13I      
DraII       EaeI        EcoO109I    EcoRII      EcoT14I     
FauI        Fnu4HI      HaeIII      HapII       HgaI        
HhaI        Hin1I       HinP1I      HpaII       HphI        
ItaI        MboII       MnlI        MspA1I      MspI        
MvaI        MvnI        MwoI        NaeI        NciI        
NgoMIV      NlaIII      NlaIV       RsaI        Sau96I      
ScrFI       StyI        TaqI        TseI        TspRI       
TthHB8I     
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