Restriction map for pF1KB6473
##################################
# NO CUT ENZYMES (  154 enzymes) #
##################################

AatII       Acc65I      AccI        AclI        AcyI        
AflIII      AgeI        AhdI        Alw44I      AlwI        
ApaI        ApaLI       AscI        AseI        AsnI        
Asp700I     Asp718I     AspEI       AspHI       AspI        
AvaI        AviII       AvrII       BamHI       BanI        
BanII       BbeI        BbrPI       BbsI        BbvCI       
BcgI        BciVI       BclI        BcnI        BglI        
BglII       BlnI        BlpI        BmyI        BpmI        
Bpu1102I    BpuAI       BsaAI       BsaBI       BsaHI       
BseRI       BsgI        BsiEI       BsiHKAI     BsiWI       
BsmBI       BsoBI       Bsp1286I    Bsp1407I    BspHI       
BspLU11I    BsrBI       BsrDI       BsrFI       BsrGI       
BssHII      BssSI       Bst1107I    BstAPI      BstBI       
BstEII      BstPI       BstYI       BstZ17I     CelII       
Cfr10I      CpoI        DraI        DraIII      DsaI        
EagI        Eam1105I    EclXI       Eco52I      EcoO65I     
EcoT22I     FbaI        FseI        FspI        HgaI        
Hin1I       HincII      HindII      HindIII     HpaI        
KasI        KpnI        KspI        MaeII       MamI        
MfeI        MflI        MluI        MunI        NaeI        
NarI        NciI        NcoI        NdeI        NgoMIV      
NheI        NotI        NruI        NsiI        NspV        
PacI        PaeR7I      PflFI       PflMI       PinAI       
PmaCI       PmeI        PmlI        PshAI       PshBI       
Psp1406I    PstI        PvuI        RcaI        RsrII       
SacI        SacII       SalI        SapI        SbfI        
SexAI       SfcI        SfiI        SfoI        SfuI        
SgrAI       SmaI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       SspI        SwaI        TfiI        
Tth111I     Van91I      XbaI        XcmI        XhoI        
XhoII       XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   49 enzymes) #
###################################

AccII         607  CGCG                 CG^CG
AccIII        273  TCCGGA               T^CCGGA
AflII         943  CTTAAG               C^TTAAG
AlwNI         433  CAGCCTCTG            CAG[ACGT][ACGT][ACGT]^CTG
AocI          962  CCTTAGG              CC^T[ACGT]AGG
Aor51HI        32  AGCGCT               AGC^GCT
AvaII         567  GGTCC                G^G[AT]CC
BfaI          952  CTAG                 C^TAG
BfrI          943  CTTAAG               C^TTAAG
BsaWI         273  TCCGGA               [AT]^CCGG[AT]
BseAI         273  TCCGGA               T^CCGGA
BsmFI         568  GTCCC                GGGAC(10/14)
BsmI          333  GAATGC               GAATGC(1/-1)
BspDI         424  ATCGAT               AT^CGAT
BspEI         273  TCCGGA               T^CCGGA
BspMI         838  ACCTGC               ACCTGC(4/8)
BsrI          892  CCAGT                ACTGG(1/-1)
BstUI         607  CGCG                 CG^CG
Bsu36I        962  CCTTAGG              CC^T[ACGT]AGG
ClaI          424  ATCGAT               AT^CGAT
DpnII          66  GATC                 ^GATC
DraII         566  GGGTCCC              [GA]G^G[ACGT]CC[CT]
DrdI          344  GACACCTGTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI          145  CTCTTC               CTCTTC(1/4)
Eco47III       32  AGCGCT               AGC^GCT
Eco81I        962  CCTTAGG              CC^T[ACGT]AGG
EcoO109I      566  GGGTCCC              [GA]G^G[ACGT]CC[CT]
EcoRI         226  GAATTC               G^AATTC
EcoRV         316  GATATC               GAT^ATC
EcoT14I       387  CCTTGG               C^C[AT][AT]GG
HapII         274  CCGG                 C^CGG
HinfI         143  GACTC                G^A[ACGT]TC
HpaII         274  CCGG                 C^CGG
Ksp632I       145  CTCTTC               CTCTTC(1/4)
MaeI          952  CTAG                 C^TAG
MaeIII        290  GTGAC                ^GT[ACGT]AC
MboI           66  GATC                 ^GATC
MroI          273  TCCGGA               T^CCGGA
MspI          274  CCGG                 C^CGG
MvnI          607  CGCG                 CG^CG
NdeII          66  GATC                 ^GATC
NspI          636  GCATGT               [GA]CATG^[CT]
PleI          143  GACTC                GAGTC(4/5)
PpuMI         566  GGGTCCC              [GA]G^G[AT]CC[CT]
PvuII         264  CAGCTG               CAG^CTG
Sau3AI         66  GATC                 ^GATC
ScaI          170  AGTACT               AGT^ACT
StyI          387  CCTTGG               C^C[AT][AT]GG
Tsp45I        290  GTGAC                ^GT[GC]AC


###################################
# TWO CUT ENZYMES (   24 enzymes) #
###################################

AcsI          226  GAATTC               [GA]^AATT[CT]
              916  AAATTT              
AfaI           90  GTAC                 GT^AC
              171  GTAC                
ApoI          226  GAATTC               [GA]^AATT[CT]
              916  AAATTT              
BalI            2  TGGCCA               TGG^CCA
               14  TGGCCA              
BbvI          432  GCAGC                GCAGC(8/12)
               35  GCTGC               
BsaI          958  GAGACC               GGTCTC(1/5)
              789  GGTCTC              
BsaJI          11  CCCTGG               C^C[ACGT][ACGT]GG
              387  CCTTGG              
BstF5I        271  CATCC                GGATG(2/0)
              463  CATCC               
BstXI           5  CCAACGCCCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              735  CCAGCTATTTGG        
Cfr13I        567  GGTCC                G^G[ACGT]CC
              593  GGCCC               
EcoNI         294  CCTCTGCCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
              962  CCTTAGGAAGG         
FauI           55  CCCGC                CCCGC(4/6)
              491  GCGGG               
FokI          271  CATCC                GGATG(9/13)
              463  CATCC               
HaeII          19  AGCGCC               [GA]GCGC^[CT]
               32  AGCGCT              
MluNI           2  TGGCCA               TGG^CCA
               14  TGGCCA              
MscI            2  TGGCCA               TGG^CCA
               14  TGGCCA              
MslI          214  CATCAGTGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              364  CATAATCGTG          
NlaIV         566  GGGTCC               GG[ACGT]^[ACGT]CC
              567  GGTCCC              
RsaI           90  GTAC                 GT^AC
              171  GTAC                
Sau96I        567  GGTCC                G^G[ACGT]CC
              593  GGCCC               
SmlI          859  CTCAAG               C^T[CT][GA]AG
              943  CTTAAG              
TaqI          425  TCGA                 T^CGA
              599  TCGA                
TseI           35  GCTGC                G^C[AT]GC
              432  GCAGC               
TthHB8I       425  TCGA                 T^CGA
              599  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   31 enzymes) #
#########################################

AciI        AluI        BsiYI       BslI        BsmAI       
BssKI       BstNI       Cac8I       CfoI        DdeI        
EaeI        EcoRII      Fnu4HI      HaeIII      HhaI        
HinP1I      HphI        ItaI        MboII       MnlI        
MseI        MspA1I      MvaI        MwoI        NlaIII      
ScrFI       SfaNI       StuI        Tru9I       Tsp509I     
TspRI       
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