Restriction map for pF1KB6473
 (Linear) (Six-base) MAPSORT of: KIBB6473.seq  Check: 367  from: 1  to: 975



KIBB6473.orf

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:47  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     952     975 
   Size:        952      23
 
AfeI AGC'GCT

Cuts at:      0      34     975 
   Size:         34     941
 
AflII C'TTAA_G

Cuts at:      0     943     975 
   Size:        943      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     438     975 
   Size:        438     537
 
ApoI r'AATT_y

Cuts at:      0     226     916     975 
   Size:        226     690      59

  Fragments arranged by size: 

                 690     226      59
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     844     975 
   Size:        844     131
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     795     952     975 
   Size:        795     157      23

  Fragments arranged by size: 

                 795     157      23
 
BsaWI w'CCGG_w

Cuts at:      0     273     975 
   Size:        273     702
 
BseYI C'CCAG_C

Cuts at:      0     337     975 
   Size:        337     638
 
BsmI GAATG_Cn'

Cuts at:      0     339     975 
   Size:        339     636
 
BspEI T'CCGG_A

Cuts at:      0     273     975 
   Size:        273     702
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     847     975 
   Size:        847     128
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0      12     742     975 
   Size:         12     730     233

  Fragments arranged by size: 

                 730     233      12
 
Bsu36I CC'TnA_GG

Cuts at:      0     963     975 
   Size:        963      12
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     670     975 
   Size:        670     305
 
BtsI GCAGTG_nn'

Cuts at:      0     106     637     759     975 
   Size:        106     531     122     216

  Fragments arranged by size: 

                 531     216     122     106
 
ClaI AT'CG_AT

Cuts at:      0     425     975 
   Size:        425     550
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     350     975 
   Size:        350     625
 
EaeI y'GGCC_r

Cuts at:      0       2      14     237     975 
   Size:          2      12     223     738

  Fragments arranged by size: 

                 738     223      12       2
 
EarI CTCTTCn'nnn_

Cuts at:      0     151     975 
   Size:        151     824
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     952     975 
   Size:        952      23
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     298     966     975 
   Size:        298     668       9

  Fragments arranged by size: 

                 668     298       9
 
EcoO109I rG'GnC_Cy

Cuts at:      0     567     975 
   Size:        567     408
 
EcoRI G'AATT_C

Cuts at:      0     226     975 
   Size:        226     749
 
EcoRV GAT'ATC

Cuts at:      0     318     975 
   Size:        318     657
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0      63     975 
   Size:         63     912
 
HaeII r_GCGC'y

Cuts at:      0      23      36     975 
   Size:         23      13     939

  Fragments arranged by size: 

                 939      23      13
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     581     613     775     807     975 
   Size:        581      32     162      32     168

  Fragments arranged by size: 

                 581     168     162      32      32
 
MscI TGG'CCA

Cuts at:      0       4      16     975 
   Size:          4      12     959

  Fragments arranged by size: 

                 959      12       4
 
MslI CAynn'nnrTG

Cuts at:      0     218     368     975 
   Size:        218     150     607

  Fragments arranged by size: 

                 607     218     150
 
MspA1I CmG'CkG

Cuts at:      0      58     266     287     975 
   Size:         58     208      21     688

  Fragments arranged by size: 

                 688     208      58      21
 
NspI r_CATG'y

Cuts at:      0     640     975 
   Size:        640     335
 
PpuMI rG'GwC_Cy

Cuts at:      0     567     975 
   Size:        567     408
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     442     474     975 
   Size:        442      32     501

  Fragments arranged by size: 

                 501     442      32
 
PvuII CAG'CTG

Cuts at:      0     266     975 
   Size:        266     709
 
SanDI GG'GwC_CC

Cuts at:      0     567     975 
   Size:        567     408
 
ScaI AGT'ACT

Cuts at:      0     172     975 
   Size:        172     803
 
SmlI C'TyrA_G

Cuts at:      0     859     943     975 
   Size:        859      84      32

  Fragments arranged by size: 

                 859      84      32
 
StuI AGG'CCT

Cuts at:      0     386     707     808     975 
   Size:        386     321     101     167

  Fragments arranged by size: 

                 386     321     167     101
 
StyI C'CwwG_G

Cuts at:      0     387     975 
   Size:        387     588
 
TatI w'GTAC_w

Cuts at:      0     170     975 
   Size:        170     805
 

 Enzymes that do cut:

     AcuI     AfeI    AflII    AlwNI     ApoI    BpuEI     BsaI    BsaWI
    BseYI     BsmI    BspEI    BspMI    BstXI   Bsu36I    BtgZI     BtsI
     ClaI     DrdI     EaeI     EarI  Eco57MI    EcoNI EcoO109I    EcoRI
    EcoRV     FalI    HaeII    Hin4I     MscI     MslI   MspA1I     NspI
    PpuMI     PsrI    PvuII    SanDI     ScaI     SmlI     StuI     StyI
     TatI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI   AflIII     AgeI     AhdI
     AleI     AlfI     AloI     ApaI    ApaLI     AscI     AseI    AsiSI
     AvaI    AvrII     BaeI    BamHI     BanI    BanII     BbeI     BbsI
    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI
 Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI   Bpu10I    BsaAI
    BsaBI    BsaHI    BsaXI    BseRI     BsgI    BsiEI  BsiHKAI    BsiWI
    BsmBI Bsp1286I    BspHI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII
    BssSI   BstAPI    BstBI   BstEII    BstYI  BstZ17I     BtgI    CspCI
     DraI   DraIII     EagI     EciI  EcoICRI     FseI     FspI    FspAI
   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI
     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI
     PpiI    PshAI     PsiI   PspOMI    PspXI     PstI     PvuI    RsrII
     SacI    SacII     SalI     SapI     SbfI    SexAI     SfcI     SfiI
     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI
     SspI     SwaI    TaqII     TstI  Tth111I     XbaI     XcmI     XhoI
     XmaI     XmnI     ZraI
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