Restriction map for
pF1KB6327
(Linear) (Six-base) MAPSORT of: KIBB6327.seq Check: 7273 from: 1 to: 783
KIBB6327.orf
With 182 enzymes: SgfI *
June 26, 2008 10:46 ..
Acc65I G'GTAC_C
Cuts at: 0 617 783
Size: 617 166
AleI CACnn'nnGTG
Cuts at: 0 190 783
Size: 190 593
ApaI G_GGCC'C
Cuts at: 0 397 783
Size: 397 386
AvaI C'yCGr_G
Cuts at: 0 313 783
Size: 313 470
BanI G'GyrC_C
Cuts at: 0 617 783
Size: 617 166
BanII G_rGCy'C
Cuts at: 0 239 320 397 783
Size: 239 81 77 386
Fragments arranged by size:
386 239 81 77
BbsI GAAGACnn'nnnn_
Cuts at: 0 340 783
Size: 340 443
BciVI GTATCCnnnnn_n'
Cuts at: 0 47 783
Size: 47 736
BglI GCCn_nnn'nGGC
Cuts at: 0 392 783
Size: 392 391
Bme1580I G_kGCm'C
Cuts at: 0 397 504 783
Size: 397 107 279
Fragments arranged by size:
397 279 107
BmrI ACTGGGnnnn_n'
Cuts at: 0 534 568 783
Size: 534 34 215
Fragments arranged by size:
534 215 34
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 223 304 487 783
Size: 223 81 183 296
Fragments arranged by size:
296 223 183 81
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 205 783
Size: 205 578
BsaI GGTCTCn'nnnn_
Cuts at: 0 91 666 783
Size: 91 575 117
Fragments arranged by size:
575 117 91
BsaAI yAC'GTr
Cuts at: 0 771 783
Size: 771 12
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 646 676 783
Size: 646 30 107
Fragments arranged by size:
646 107 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 277 385 783
Size: 277 108 398
Fragments arranged by size:
398 277 108
BseYI C'CCAG_C
Cuts at: 0 521 783
Size: 521 262
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 715 783
Size: 715 68
BsiHKAI G_wGCw'C
Cuts at: 0 239 320 783
Size: 239 81 463
Fragments arranged by size:
463 239 81
Bsp1286I G_dGCh'C
Cuts at: 0 239 320 397 504 783
Size: 239 81 77 107 279
Fragments arranged by size:
279 239 107 81 77
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 109 783
Size: 109 674
BsrDI GCAATG_nn'
Cuts at: 0 90 783
Size: 90 693
BsrFI r'CCGG_y
Cuts at: 0 455 531 739 759 783
Size: 455 76 208 20 24
Fragments arranged by size:
455 208 76 24 20
BstXI CCAn_nnnn'nTGG
Cuts at: 0 547 771 783
Size: 547 224 12
Fragments arranged by size:
547 224 12
BtgI C'CryG_G
Cuts at: 0 41 188 783
Size: 41 147 595
Fragments arranged by size:
595 147 41
DrdI GACnn_nn'nnGTC
Cuts at: 0 685 783
Size: 685 98
EaeI y'GGCC_r
Cuts at: 0 761 783
Size: 761 22
EarI CTCTTCn'nnn_
Cuts at: 0 376 783
Size: 376 407
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 211 783
Size: 211 572
EcoICRI GAG'CTC
Cuts at: 0 237 318 783
Size: 237 81 465
Fragments arranged by size:
465 237 81
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 223 304 487 783
Size: 223 81 183 296
Fragments arranged by size:
296 223 183 81
EcoO109I rG'GnC_Cy
Cuts at: 0 394 783
Size: 394 389
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 198 783
Size: 198 585
HaeII r_GCGC'y
Cuts at: 0 596 644 783
Size: 596 48 139
Fragments arranged by size:
596 139 48
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 21 53 646 678 783
Size: 21 32 593 32 105
Fragments arranged by size:
593 105 32 32 21
HincII GTy'rAC
Cuts at: 0 690 783
Size: 690 93
KpnI G_GTAC'C
Cuts at: 0 621 783
Size: 621 162
MslI CAynn'nnrTG
Cuts at: 0 190 751 783
Size: 190 561 32
Fragments arranged by size:
561 190 32
NaeI GCC'GGC
Cuts at: 0 761 783
Size: 761 22
NcoI C'CATG_G
Cuts at: 0 188 783
Size: 188 595
NgoMIV G'CCGG_C
Cuts at: 0 759 783
Size: 759 24
NspI r_CATG'y
Cuts at: 0 86 783
Size: 86 697
PflMI CCAn_nnn'nTGG
Cuts at: 0 604 783
Size: 604 179
PfoI T'CCnGG_A
Cuts at: 0 668 783
Size: 668 115
PspOMI G'GGCC_C
Cuts at: 0 393 783
Size: 393 390
PstI C_TGCA'G
Cuts at: 0 570 783
Size: 570 213
SacI G_AGCT'C
Cuts at: 0 239 320 783
Size: 239 81 463
Fragments arranged by size:
463 239 81
SapI GCTCTTCn'nnn_
Cuts at: 0 376 783
Size: 376 407
SfcI C'TryA_G
Cuts at: 0 566 636 783
Size: 566 70 147
Fragments arranged by size:
566 147 70
SgrAI Cr'CCGG_yG
Cuts at: 0 739 783
Size: 739 44
SmlI C'TyrA_G
Cuts at: 0 220 783
Size: 220 563
SphI G_CATG'C
Cuts at: 0 86 783
Size: 86 697
StyI C'CwwG_G
Cuts at: 0 188 783
Size: 188 595
Tth111I GACn'n_nGTC
Cuts at: 0 658 783
Size: 658 125
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 547 783
Size: 547 236
XmnI GAAnn'nnTTC
Cuts at: 0 212 783
Size: 212 571
Enzymes that do cut:
Acc65I AleI ApaI AvaI BanI BanII BbsI BciVI
BglI Bme1580I BmrI BpmI BpuEI BsaI BsaAI BsaXI
BseRI BseYI BsgI BsiHKAI Bsp1286I BspMI BsrDI BsrFI
BstXI BtgI DrdI EaeI EarI EciI EcoICRI Eco57MI
EcoO109I FalI HaeII Hin4I HincII KpnI MslI NaeI
NcoI NgoMIV NspI PflMI PfoI PspOMI PstI SacI
SapI SfcI SgrAI SmlI SphI StyI Tth111I XcmI
XmnI
Enzymes that do not cut:
AarI AatII AccI AclI AcuI AfeI AflII AflIII
AgeI AhdI AlfI AloI AlwNI ApaLI ApoI AscI
AseI AsiSI AvrII BaeI BamHI BbeI BbvCI BcgI
BclI BfrBI BglII BlpI BmgBI BmtI BplI Bpu10I
BsaBI BsaHI BsaWI BsiEI BsiWI BsmI BsmBI BspEI
BspHI BsrBI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraI
DraIII EagI EcoNI EcoRI EcoRV FseI FspI FspAI
HindIII HpaI KasI MfeI MluI MmeI MscI MspA1I
NarI NdeI NheI NotI NruI NsiI PacI PasI
PciI PmeI PmlI PpiI PpuMI PshAI PsiI PspXI
PsrI PvuI PvuII RsrII SacII SalI SanDI SbfI
ScaI SexAI SfiI SfoI SgfI SmaI SnaBI SpeI
SrfI SspI StuI SwaI TaqII TatI TstI XbaI
XhoI XmaI ZraI