Restriction map for pF1KB6333
##################################
# NO CUT ENZYMES (  148 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcsI        AcyI        AflII       AgeI        
AhdI        Aor51HI     ApoI        AscI        AseI        
AsnI        Asp718I     AspEI       AspI        AvrII       
BamHI       BanI        BbeI        BbrPI       BbvCI       
BcgI        BciVI       BclI        BfaI        BfrI        
BglII       BlnI        BlpI        Bpu1102I    BsaAI       
BsaBI       BsaHI       BsaI        BseAI       BsiEI       
BsiWI       BsmBI       BspEI       BspHI       BspLU11I    
BsrDI       BsrFI       BssHII      Bst1107I    BstAPI      
BstBI       BstUI       BstXI       BstYI       BstZ17I     
CelII       Cfr10I      CpoI        DraI        DraIII      
DrdI        DsaI        EagI        Eam1105I    EarI        
EclXI       Eco47III    Eco52I      EcoNI       EcoRI       
EcoRV       EcoT22I     FbaI        FseI        HaeII       
Hin1I       HincII      HindII      HindIII     HpaI        
KasI        KpnI        Ksp632I     KspI        MaeI        
MamI        MfeI        MflI        MluI        MroI        
MseI        MunI        MvnI        NaeI        NarI        
NcoI        NdeI        NgoMIV      NheI        NotI        
NruI        NsiI        NspI        NspV        PacI        
PaeR7I      PflFI       PflMI       PinAI       PmaCI       
PmeI        PmlI        PshAI       PshBI       Psp1406I    
PstI        PvuI        RcaI        RsrII       SacII       
SalI        SapI        SbfI        ScaI        SexAI       
SfcI        SfiI        SfoI        SfuI        SgrAI       
SmaI        SmlI        SnaBI       SpeI        SphI        
Sse8387I    SspI        SwaI        Tru9I       Tsp509I     
Tth111I     Van91I      XbaI        XcmI        XhoI        
XhoII       XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   51 enzymes) #
###################################

AfaI          726  GTAC                 GT^AC
AflIII        545  ACGTGT               A^C[GA][CT]GT
Alw44I        163  GTGCAC               G^TGCAC
AlwI          755  GGATC                GGATC(4/5)
AlwNI         380  CAGAGCCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApaI          642  GGGCCC               GGGCC^C
ApaLI         163  GTGCAC               G^TGCAC
Asp700I       321  GAATCGATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI          627  CTCGGG               C^[CT]CG[GA]G
AviII         552  TGCGCA               TGC^GCA
BalI          773  TGGCCA               TGG^CCA
BcnI            9  CCCGG                CC^[GC]GG
BglI          708  GCCCGAAAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI          348  CTCCAG               CTGGAG(16/14)
BsaWI         746  ACCGGA               [AT]^CCGG[AT]
BseRI         487  GAGGAG               GAGGAG(10/8)
BsgI           50  CTGCAC               GTGCAG(16/14)
BsmAI          66  GTCTC                GTCTC(1/5)
BsmI           99  GCATTC               GAATGC(1/-1)
BsoBI         627  CTCGGG               C^[CT]CG[GA]G
Bsp1407I      725  TGTACA               T^GTACA
BspDI         323  ATCGAT               AT^CGAT
BsrBI         295  CCGCTC               CCGCTC(-3/-3)
BsrGI         725  TGTACA               T^GTACA
BssSI         131  CTCGTG               CACGAG(-5/-1)
BstEII        573  GGTGACC              G^GT[ACGT]ACC
BstPI         573  GGTGACC              G^GT[ACGT]ACC
CfoI          553  GCGC                 GCG^C
ClaI          323  ATCGAT               AT^CGAT
EaeI          773  TGGCCA               [CT]^GGCC[GA]
EcoO65I       573  GGTGACC              G^GT[ACGT]ACC
EcoT14I       731  CCAAGG               C^C[AT][AT]GG
FauI          294  CCCGC                CCCGC(4/6)
FspI          552  TGCGCA               TGC^GCA
HgaI          599  GACGC                GACGC(5/10)
HhaI          553  GCGC                 GCG^C
HinP1I        553  GCGC                 G^CGC
MluNI         773  TGGCCA               TGG^CCA
MscI          773  TGGCCA               TGG^CCA
MspA1I        185  CAGCTG               C[AC]G^C[GT]G
NciI            9  CCCGG                CC^[GC]GG
PleI          346  GACTC                GAGTC(4/5)
PvuII         185  CAGCTG               CAG^CTG
RsaI          726  GTAC                 GT^AC
SacI          391  GAGCTC               GAGCT^C
SspBI         725  TGTACA               T^GTACA
StuI          715  AGGCCT               AGG^CCT
StyI          731  CCAAGG               C^C[AT][AT]GG
TaqI          324  TCGA                 T^CGA
TthHB8I       324  TCGA                 T^CGA
XmnI          321  GAATCGATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   23 enzymes) #
###################################

AocI          330  CCTCAGG              CC^T[ACGT]AGG
              461  CCTCAGG             
AspHI         163  GTGCAC               G[AT]GC[AT]^C
              391  GAGCTC              
BanII         391  GAGCTC               G[GA]GC[CT]^C
              642  GGGCCC              
BbsI          250  GAAGAC               GAAGAC(2/6)
               16  GTCTTC              
BpuAI         250  GAAGAC               GAAGAC(2/6)
               16  GTCTTC              
BsiHKAI       163  GTGCAC               G[AT]GC[AT]^C
              391  GAGCTC              
BsmFI         445  GGGAC                GGGAC(10/14)
                7  GTCCC               
BsrI          441  ACTGG                ACTGG(1/-1)
              171  CCAGT               
Bsu36I        330  CCTCAGG              CC^T[ACGT]AGG
              461  CCTCAGG             
Cac8I         384  GCCTGC               GC[ACGT]^[ACGT]GC
              435  GCCAGC              
DpnII         216  GATC                 ^GATC
              756  GATC                
Eco81I        330  CCTCAGG              CC^T[ACGT]AGG
              461  CCTCAGG             
HapII          10  CCGG                 C^CGG
              747  CCGG                
HpaII          10  CCGG                 C^CGG
              747  CCGG                
MaeIII         34  GTGAC                ^GT[ACGT]AC
              574  GTGAC               
MboI          216  GATC                 ^GATC
              756  GATC                
MslI          729  CACCAAGGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              765  CACCATCGTG          
MspI           10  CCGG                 C^CGG
              747  CCGG                
NdeII         216  GATC                 ^GATC
              756  GATC                
PpuMI           5  GGGTCCC              [GA]G^G[AT]CC[CT]
              176  GGGTCCT             
Sau3AI        216  GATC                 ^GATC
              756  GATC                
SfaNI         400  GATGC                GCATC(5/9)
              197  GCATC               
Tsp45I         34  GTGAC                ^GT[GC]AC
              574  GTGAC               


#########################################
# 3 OR MORE CUT ENZYMES (   36 enzymes) #
#########################################

AciI        AluI        AvaII       BbvI        BmyI        
BsaJI       BsiYI       BslI        Bsp1286I    BspMI       
BssKI       BstF5I      BstNI       Cfr13I      DdeI        
DraII       EcoO109I    EcoRII      Fnu4HI      FokI        
HaeIII      HinfI       HphI        ItaI        MaeII       
MboII       MnlI        MvaI        MwoI        NlaIII      
NlaIV       Sau96I      ScrFI       TfiI        TseI        
TspRI       
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