Restriction map for pF1KB7618
 (Linear) (Six-base) MAPSORT of: KIBB7618.seq  Check: 3000  from: 1  to: 1086



KIBB7618.orf

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:49  ..

Acc65I G'GTAC_C

Cuts at:      0     605    1086 
   Size:        605     481
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     907    1086 
   Size:        907     179
 
AflIII A'CryG_T

Cuts at:      0     836    1086 
   Size:        836     250
 
AgeI A'CCGG_T

Cuts at:      0     501    1086 
   Size:        501     585
 
AlwNI CAG_nnn'CTG

Cuts at:      0     568    1086 
   Size:        568     518
 
ApaI G_GGCC'C

Cuts at:      0      55     121     253     451     518    1086 
   Size:         55      66     132     198      67     568

  Fragments arranged by size: 

                 568     198     132      67      66      55
 
AscI GG'CGCG_CC

Cuts at:      0     266    1086 
   Size:        266     820
 
AvaI C'yCGr_G

Cuts at:      0     120     175     246     328     357     396     425     475 
   Size:        120      55      71      82      29      39      29      50

Cuts at:    475     510     531     721     742     849    1086 
   Size:         35      21     190      21     107     237

  Fragments arranged by size: 

                 237     190     120     107      82      71      55      50
                  39      35      29      29      21      21
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     490     523    1086 
   Size:        490      33     563

  Fragments arranged by size: 

                 563     490      33
 
BamHI G'GATC_C

Cuts at:      0     759    1086 
   Size:        759     327
 
BanI G'GyrC_C

Cuts at:      0     288     520     580     605     624    1086 
   Size:        288     232      60      25      19     462

  Fragments arranged by size: 

                 462     288     232      60      25      19
 
BanII G_rGCy'C

Cuts at:      0      55     121     253     260     426     432     451     482 
   Size:         55      66     132       7     166       6      19      31

Cuts at:    482     518     665     880    1086 
   Size:         36     147     215     206

  Fragments arranged by size: 

                 215     206     166     147     132      66      55      36
                  31      19       7       6
 
BbeI G_GCGC'C

Cuts at:      0     292     524     584     628    1086 
   Size:        292     232      60      44     458

  Fragments arranged by size: 

                 458     292     232      60      44
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     723     757    1086 
   Size:        723      34     329

  Fragments arranged by size: 

                 723     329      34
 
BglI GCCn_nnn'nGGC

Cuts at:      0     307    1086 
   Size:        307     779
 
Bme1580I G_kGCm'C

Cuts at:      0      55     121     253     451     518     644     736    1086 
   Size:         55      66     132     198      67     126      92     350

  Fragments arranged by size: 

                 350     198     132     126      92      67      66      55
            
 
BmgBI CAC'GTC

Cuts at:      0     692    1086 
   Size:        692     394
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     411    1086 
   Size:        411     675
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     446     649     972    1086 
   Size:        446     203     323     114

  Fragments arranged by size: 

                 446     323     203     114
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      67    1086 
   Size:         67    1019
 
BsaI GGTCTCn'nnnn_

Cuts at:      0       7     772    1086 
   Size:          7     765     314

  Fragments arranged by size: 

                 765     314       7
 
BsaAI yAC'GTr

Cuts at:      0     156     839     935    1036    1086 
   Size:        156     683      96     101      50

  Fragments arranged by size: 

                 683     156     101      96      50
 
BsaHI Gr'CG_yC

Cuts at:      0     209     289     437     521     574     581     625    1086 
   Size:        209      80     148      84      53       7      44     461

  Fragments arranged by size: 

                 461     209     148      84      80      53      44       7
            
 
BsaWI w'CCGG_w

Cuts at:      0     501     989    1086 
   Size:        501     488      97

  Fragments arranged by size: 

                 501     488      97
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     220     223     519    1086 
   Size:        220       3     296     567

  Fragments arranged by size: 

                 567     296     220       3
 
BseYI C'CCAG_C

Cuts at:      0     292     370     450    1086 
   Size:        292      78      80     636

  Fragments arranged by size: 

                 636     292      80      78
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     915    1086 
   Size:        915     171
 
BsiHKAI G_wGCw'C

Cuts at:      0     880    1086 
   Size:        880     206
 
Bsp1286I G_dGCh'C

Cuts at:      0      55     121     253     260     426     432     451     482 
   Size:         55      66     132       7     166       6      19      31

Cuts at:    482     518     644     665     736     880    1086 
   Size:         36     126      21      71     144     206

  Fragments arranged by size: 

                 206     166     144     132     126      71      66      55
                  36      31      21      19       7       6
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     286    1086 
   Size:        286     800
 
BsrFI r'CCGG_y

Cuts at:      0     501     523    1086 
   Size:        501      22     563

  Fragments arranged by size: 

                 563     501      22
 
BssHII G'CGCG_C

Cuts at:      0     266     814     842     961    1086 
   Size:        266     548      28     119     125

  Fragments arranged by size: 

                 548     266     125     119      28
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     489    1086 
   Size:        489     597
 
BstYI r'GATC_y

Cuts at:      0     225     759    1086 
   Size:        225     534     327

  Fragments arranged by size: 

                 534     327     225
 
BtgI C'CryG_G

Cuts at:      0     137     547     657    1086 
   Size:        137     410     110     429

  Fragments arranged by size: 

                 429     410     137     110
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     628    1086 
   Size:        628     458
 
EaeI y'GGCC_r

Cuts at:      0       2    1086 
   Size:          2    1084
 
EarI CTCTTCn'nnn_

Cuts at:      0      95     179     658    1086 
   Size:         95      84     479     428

  Fragments arranged by size: 

                 479     428      95      84
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     308    1086 
   Size:        308     778
 
EcoICRI GAG'CTC

Cuts at:      0     878    1086 
   Size:        878     208
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     446     649     907     972    1086 
   Size:        446     203     258      65     114

  Fragments arranged by size: 

                 446     258     203     114      65
 
EcoO109I rG'GnC_Cy

Cuts at:      0      52     124     219     514     670     718    1086 
   Size:         52      72      95     295     156      48     368

  Fragments arranged by size: 

                 368     295     156      95      72      52      48
 
FspI TGC'GCA

Cuts at:      0     901    1086 
   Size:        901     185
 
HaeII r_GCGC'y

Cuts at:      0      96     292     490     496     524     584     628    1011 
   Size:         96     196     198       6      28      60      44     383

Cuts at:   1011    1080    1086 
   Size:         69       6

  Fragments arranged by size: 

                 383     198     196      96      69      60      44      28
                   6       6
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     267     299    1086 
   Size:        267      32     787

  Fragments arranged by size: 

                 787     267      32
 
KasI G'GCGC_C

Cuts at:      0     288     520     580     624    1086 
   Size:        288     232      60      44     462

  Fragments arranged by size: 

                 462     288     232      60      44
 
KpnI G_GTAC'C

Cuts at:      0     609    1086 
   Size:        609     477
 
MscI TGG'CCA

Cuts at:      0       4    1086 
   Size:          4    1082
 
MspA1I CmG'CkG

Cuts at:      0     549     659    1019    1086 
   Size:        549     110     360      67

  Fragments arranged by size: 

                 549     360     110      67
 
NaeI GCC'GGC

Cuts at:      0     525    1086 
   Size:        525     561
 
NarI GG'CG_CC

Cuts at:      0     289     521     581     625    1086 
   Size:        289     232      60      44     461

  Fragments arranged by size: 

                 461     289     232      60      44
 
NdeI CA'TA_TG

Cuts at:      0     345    1086 
   Size:        345     741
 
NgoMIV G'CCGG_C

Cuts at:      0     523    1086 
   Size:        523     563
 
PasI CC'CwG_GG

Cuts at:      0      48    1086 
   Size:         48    1038
 
PfoI T'CCnGG_A

Cuts at:      0      59    1086 
   Size:         59    1027
 
PmlI CAC'GTG

Cuts at:      0     156     839     935    1036    1086 
   Size:        156     683      96     101      50

  Fragments arranged by size: 

                 683     156     101      96      50
 
PpuMI rG'GwC_Cy

Cuts at:      0     124     219     670     718    1086 
   Size:        124      95     451      48     368

  Fragments arranged by size: 

                 451     368     124      95      48
 
PspOMI G'GGCC_C

Cuts at:      0      51     117     249     447     514    1086 
   Size:         51      66     132     198      67     572

  Fragments arranged by size: 

                 572     198     132      67      66      51
 
PstI C_TGCA'G

Cuts at:      0     756    1086 
   Size:        756     330
 
RsrII CG'GwC_CG

Cuts at:      0     551    1086 
   Size:        551     535
 
SacI G_AGCT'C

Cuts at:      0     880    1086 
   Size:        880     206
 
SacII CC_GC'GG

Cuts at:      0     550     660    1086 
   Size:        550     110     426

  Fragments arranged by size: 

                 550     426     110
 
SanDI GG'GwC_CC

Cuts at:      0     670     718    1086 
   Size:        670      48     368

  Fragments arranged by size: 

                 670     368      48
 
SapI GCTCTTCn'nnn_

Cuts at:      0     658    1086 
   Size:        658     428
 
SfcI C'TryA_G

Cuts at:      0     752    1086 
   Size:        752     334
 
SfoI GGC'GCC

Cuts at:      0     290     522     582     626    1086 
   Size:        290     232      60      44     460

  Fragments arranged by size: 

                 460     290     232      60      44
 
SmaI CCC'GGG

Cuts at:      0     122     177     359     427     512     723     851    1086 
   Size:        122      55     182      68      85     211     128     235

  Fragments arranged by size: 

                 235     211     182     128     122      85      68      55
            
 
SmlI C'TyrA_G

Cuts at:      0      82    1086 
   Size:         82    1004
 
SrfI GCCC'GGGC

Cuts at:      0     427    1086 
   Size:        427     659
 
StuI AGG'CCT

Cuts at:      0     301    1086 
   Size:        301     785
 
StyI C'CwwG_G

Cuts at:      0     482     636    1086 
   Size:        482     154     450

  Fragments arranged by size: 

                 482     450     154
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     393    1086 
   Size:        393     693
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     219    1086 
   Size:        219     867
 
XmaI C'CCGG_G

Cuts at:      0     120     175     357     425     510     721     849    1086 
   Size:        120      55     182      68      85     211     128     237

  Fragments arranged by size: 

                 237     211     182     128     120      85      68      55
            
 

 Enzymes that do cut:

   Acc65I     AcuI   AflIII     AgeI    AlwNI     ApaI     AscI     AvaI
     BaeI    BamHI     BanI    BanII     BbeI     BcgI     BglI Bme1580I
    BmgBI     BplI     BpmI    BpuEI     BsaI    BsaAI    BsaHI    BsaWI
    BseRI    BseYI     BsgI  BsiHKAI Bsp1286I    BspMI    BsrFI   BssHII
    BstXI    BstYI     BtgI    BtgZI     EaeI     EarI     EciI  EcoICRI
  Eco57MI EcoO109I     FspI    HaeII    Hin4I     KasI     KpnI     MscI
   MspA1I     NaeI     NarI     NdeI   NgoMIV     PasI     PfoI     PmlI
    PpuMI   PspOMI     PstI    RsrII     SacI    SacII    SanDI     SapI
     SfcI     SfoI     SmaI     SmlI     SrfI     StuI     StyI    TaqII
     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI     AclI     AfeI    AflII     AhdI     AleI
     AlfI     AloI    ApaLI     ApoI     AseI    AsiSI    AvrII     BbsI
    BbvCI    BciVI     BclI    BfrBI    BglII     BlpI     BmrI     BmtI
   Bpu10I    BsaBI    BsaXI    BsiEI    BsiWI     BsmI    BsmBI    BspEI
    BspHI    BsrBI    BsrDI    BsrGI    BssSI   BstAPI    BstBI   BstEII
  BstZ17I   Bsu36I     BtsI     ClaI    CspCI     DraI   DraIII     DrdI
     EagI    EcoNI    EcoRI    EcoRV     FalI     FseI    FspAI   HincII
  HindIII     HpaI     MfeI     MluI     MmeI     MslI     NcoI     NheI
     NotI     NruI     NsiI     NspI     PacI     PciI    PflMI     PmeI
     PpiI    PshAI     PsiI    PspXI     PsrI     PvuI    PvuII     SalI
     SbfI     ScaI    SexAI     SfiI     SgfI    SgrAI    SnaBI     SpeI
     SphI     SspI     SwaI     TatI     TstI  Tth111I     XbaI     XhoI
     XmnI     ZraI
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