Restriction map for pF1KB8573
##################################
# NO CUT ENZYMES (  100 enzymes) #
##################################

AatII       AccII       AccIII      AclI        AcyI        
AflII       AflIII      AhdI        Alw44I      AocI        
ApaI        ApaLI       AscI        AseI        AsnI        
AspEI       AviII       AvrII       BbeI        BbrPI       
BclI        BfrI        BlnI        BsaAI       BsaHI       
BseAI       BsiEI       BsiWI       BsmI        Bsp1407I    
BspEI       BspHI       BspLU11I    BsrGI       BssHII      
BssSI       BstEII      BstPI       BstUI       Bsu36I      
CpoI        DraI        DsaI        EagI        Eam1105I    
EclXI       Eco52I      Eco81I      EcoO65I     EcoRI       
EcoRV       FbaI        FseI        FspI        HgaI        
Hin1I       HindIII     HpaI        KasI        KspI        
MaeII       MfeI        MluI        MroI        MunI        
MvnI        NaeI        NarI        NcoI        NgoMIV      
NotI        NruI        PacI        PmaCI       PmeI        
PmlI        PpuMI       PshBI       Psp1406I    PvuI        
RcaI        RsrII       SacII       SalI        SbfI        
ScaI        SfiI        SfoI        SgrAI       SmaI        
SnaBI       SpeI        Sse8387I    SspBI       SspI        
SwaI        XbaI        XcmI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   50 enzymes) #
###################################

AgeI         1349  ACCGGT               A^CCGGT
Aor51HI      1238  AGCGCT               AGC^GCT
AspHI        1684  GAGCTC               G[AT]GC[AT]^C
AspI           46  GACGATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaI         1394  CTCGAG               C^[CT]CG[GA]G
BcgI          761  GCACCAGCTTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI        1591  GTATCC               GTATCC(6/5)
BglI          569  GCCTCCAAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI         2431  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I     2431  GCTGAGC              GC^T[ACGT]AGC
BsaBI        1547  GATTCCCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI          280  GAGACC               GGTCTC(1/5)
BsaWI        1349  ACCGGT               [AT]^CCGG[AT]
BsiHKAI      1684  GAGCTC               G[AT]GC[AT]^C
BsmBI         135  CGTCTC               CGTCTC(1/5)
BsoBI        1394  CTCGAG               C^[CT]CG[GA]G
BspDI         842  ATCGAT               AT^CGAT
BsrBI        1927  GAGCGG               CCGCTC(-3/-3)
BsrFI        1349  ACCGGT               [GA]^CCGG[CT]
Bst1107I      199  GTATAC               GTA^TAC
BstBI        2549  TTCGAA               TT^CGAA
BstZ17I       199  GTATAC               GTA^TAC
CelII        2431  GCTGAGC              GC^T[ACGT]AGC
Cfr10I       1349  ACCGGT               [GA]^CCGG[CT]
ClaI          842  ATCGAT               AT^CGAT
DraII         214  AGGCCCT              [GA]G^G[ACGT]CC[CT]
DraIII       1214  CACTCTGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI          124  GACTGCTCTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III     1238  AGCGCT               AGC^GCT
EcoO109I      214  AGGCCCT              [GA]G^G[ACGT]CC[CT]
EcoT22I      1756  ATGCAT               ATGCA^T
HincII       1389  GTTGAC               GT[CT]^[GA]AC
HindII       1389  GTTGAC               GT[CT]^[GA]AC
MamI         1547  GATTCCCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NdeI          473  CATATG               CA^TATG
NheI         1510  GCTAGC               G^CTAGC
NsiI         1756  ATGCAT               ATGCA^T
NspV         2549  TTCGAA               TT^CGAA
PaeR7I       1394  CTCGAG               C^TCGAG
PflFI          46  GACGATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI        2358  CCAGACTCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI        1349  ACCGGT               A^CCGGT
PshAI        1976  GACAACAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI         1684  GAGCTC               GAGCT^C
SexAI        1364  ACCTGGT              A^CC[AT]GGT
SfuI         2549  TTCGAA               TT^CGAA
SphI         1524  GCATGC               GCATG^C
Tth111I        46  GACGATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Van91I       2358  CCAGACTCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI         1394  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   17 enzymes) #
###################################

AccI          199  GTATAC               GT^[AC][GT]AC
              733  GTCTAC              
AcsI         1772  AAATTT               [GA]^AATT[CT]
             2553  AAATTT              
ApoI         1772  AAATTT               [GA]^AATT[CT]
             2553  AAATTT              
BalI          342  TGGCCA               TGG^CCA
             1490  TGGCCA              
BanII         787  GGGCTC               G[GA]GC[CT]^C
             1684  GAGCTC              
BbsI         1710  GAAGAC               GAAGAC(2/6)
             1583  GTCTTC              
BbvCI        2162  CCTCAGC              CCTCAGC(-5/-2)
             1054  GCTGAGG             
BpmI         1128  CTCCAG               CTGGAG(16/14)
             1750  CTGGAG              
BpuAI        1710  GAAGAC               GAAGAC(2/6)
             1583  GTCTTC              
BsgI         1558  CTGCAC               GTGCAG(16/14)
             1818  GTGCAG              
EaeI          342  TGGCCA               [CT]^GGCC[GA]
             1490  TGGCCA              
EcoNI         693  CCTCCAAGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
              719  CCTTGAAGAGG         
MluNI         342  TGGCCA               TGG^CCA
             1490  TGGCCA              
MscI          342  TGGCCA               TGG^CCA
             1490  TGGCCA              
MslI         1662  CATCTCCATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             2181  CATTGATGTG          
NspI         1524  GCATGC               [GA]CATG^[CT]
             2422  GCATGT              
SapI          321  GAAGAGC              GCTCTTC(1/4)
             1945  GAAGAGC             


#########################################
# 3 OR MORE CUT ENZYMES (   91 enzymes) #
#########################################

Acc65I      AciI        AfaI        AluI        AlwI        
AlwNI       Asp700I     Asp718I     AvaII       BamHI       
BanI        BbvI        BcnI        BfaI        BglII       
BmyI        BsaJI       BseRI       BsiYI       BslI        
BsmAI       BsmFI       Bsp1286I    BspMI       BsrDI       
BsrI        BssKI       BstAPI      BstF5I      BstNI       
BstXI       BstYI       Cac8I       CfoI        Cfr13I      
DdeI        DpnII       EarI        EcoRII      EcoT14I     
FauI        Fnu4HI      FokI        HaeII       HaeIII      
HapII       HhaI        HinP1I      HinfI       HpaII       
HphI        ItaI        KpnI        Ksp632I     MaeI        
MaeIII      MboI        MboII       MflI        MnlI        
MseI        MspA1I      MspI        MvaI        MwoI        
NciI        NdeII       NlaIII      NlaIV       PleI        
PstI        PvuII       RsaI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        SmlI        StuI        
StyI        TaqI        TfiI        Tru9I       TseI        
Tsp45I      Tsp509I     TspRI       TthHB8I     XhoII       
XmnI        
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