Restriction map for pF1KB8573
 (Linear) (Six-base) MAPSORT of: KIBB8573.seq  Check: 8480  from: 1  to: 2670



KIBB8573.orf

 With 182 enzymes: SgfI * 

                        March 2, 2010 14:28  ..

AccI GT'mk_AC

Cuts at:      0     200     734    2670 
   Size:        200     534    1936

  Fragments arranged by size: 

                1936     534     200
 
Acc65I G'GTAC_C

Cuts at:      0     514     754    2491    2670 
   Size:        514     240    1737     179

  Fragments arranged by size: 

                1737     514     240     179
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     212     340     622    1156    1570    2050    2141    2227 
   Size:        212     128     282     534     414     480      91      86

Cuts at:   2227    2670 
   Size:        443

  Fragments arranged by size: 

                 534     480     443     414     282     212     128      91
                  86
 
AfeI AGC'GCT

Cuts at:      0    1240    2670 
   Size:       1240    1430
 
AgeI A'CCGG_T

Cuts at:      0    1349    2670 
   Size:       1349    1321
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     130    2670 
   Size:        130    2540
 
AlwNI CAG_nnn'CTG

Cuts at:      0     862    1365    2008    2047    2364    2670 
   Size:        862     503     643      39     317     306

  Fragments arranged by size: 

                 862     643     503     317     306      39
 
ApoI r'AATT_y

Cuts at:      0    1772    2553    2670 
   Size:       1772     781     117

  Fragments arranged by size: 

                1772     781     117
 
AvaI C'yCGr_G

Cuts at:      0    1394    2670 
   Size:       1394    1276
 
BamHI G'GATC_C

Cuts at:      0      11    1160    2612    2670 
   Size:         11    1149    1452      58

  Fragments arranged by size: 

                1452    1149      58      11
 
BanI G'GyrC_C

Cuts at:      0     514     754    2491    2670 
   Size:        514     240    1737     179

  Fragments arranged by size: 

                1737     514     240     179
 
BanII G_rGCy'C

Cuts at:      0     791    1688    2670 
   Size:        791     897     982

  Fragments arranged by size: 

                 982     897     791
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1576    1717    2670 
   Size:       1576     141     953

  Fragments arranged by size: 

                1576     953     141
 
BbvCI CC'TCA_GC

Cuts at:      0    1055    2163    2670 
   Size:       1055    1108     507

  Fragments arranged by size: 

                1108    1055     507
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     750     784    2670 
   Size:        750      34    1886

  Fragments arranged by size: 

                1886     750      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1602    2670 
   Size:       1602    1068
 
BfrBI ATG'CAT

Cuts at:      0    1758    2670 
   Size:       1758     912
 
BglI GCCn_nnn'nGGC

Cuts at:      0     575    2670 
   Size:        575    2095
 
BglII A'GATC_T

Cuts at:      0     374     920    1026    2670 
   Size:        374     546     106    1644

  Fragments arranged by size: 

                1644     546     374     106
 
BlpI GC'TnA_GC

Cuts at:      0    2432    2670 
   Size:       2432     238
 
Bme1580I G_kGCm'C

Cuts at:      0     852    2670 
   Size:        852    1818
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1552    2306    2670 
   Size:       1552     754     364

  Fragments arranged by size: 

                1552     754     364
 
BmtI G_CTAG'C

Cuts at:      0    1514    2670 
   Size:       1514    1156
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     553    2127    2670 
   Size:        553    1574     543

  Fragments arranged by size: 

                1574     553     543
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1113    1771    2670 
   Size:       1113     658     899

  Fragments arranged by size: 

                1113     899     658
 
Bpu10I CC'TnA_GC

Cuts at:      0    1055    1616    2163    2670 
   Size:       1055     561     547     507

  Fragments arranged by size: 

                1055     561     547     507
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     225    1870    2670 
   Size:        225    1645     800

  Fragments arranged by size: 

                1645     800     225
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     274    2670 
   Size:        274    2396
 
BsaBI GATnn'nnATC

Cuts at:      0    1551    2670 
   Size:       1551    1119
 
BsaWI w'CCGG_w

Cuts at:      0    1349    2670 
   Size:       1349    1321
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     694     724    1111    1141    1175    1205    1846    1876 
   Size:        694      30     387      30      34      30     641      30

Cuts at:   1876    2670 
   Size:        794

  Fragments arranged by size: 

                 794     694     641     387      34      30      30      30
                  30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     715     718     741    1072    1696    2670 
   Size:        715       3      23     331     624     974

  Fragments arranged by size: 

                 974     715     624     331      23       3
 
BseYI C'CCAG_C

Cuts at:      0     784    1597    2057    2670 
   Size:        784     813     460     613

  Fragments arranged by size: 

                 813     784     613     460
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1543    1839    2670 
   Size:       1543     296     831

  Fragments arranged by size: 

                1543     831     296
 
BsiHKAI G_wGCw'C

Cuts at:      0    1688    2670 
   Size:       1688     982
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     141    2670 
   Size:        141    2529
 
Bsp1286I G_dGCh'C

Cuts at:      0     791     852    1688    2670 
   Size:        791      61     836     982

  Fragments arranged by size: 

                 982     836     791      61
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     122     153    2584    2670 
   Size:        122      31    2431      86

  Fragments arranged by size: 

                2431     122      86      31
 
BsrBI CCG'CTC

Cuts at:      0    1929    2670 
   Size:       1929     741
 
BsrDI GCAATG_nn'

Cuts at:      0     333     779    1505    1785    2670 
   Size:        333     446     726     280     885

  Fragments arranged by size: 

                 885     726     446     333     280
 
BsrFI r'CCGG_y

Cuts at:      0    1349    2670 
   Size:       1349    1321
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     984    2047    2428    2670 
   Size:        984    1063     381     242

  Fragments arranged by size: 

                1063     984     381     242
 
BstBI TT'CG_AA

Cuts at:      0    2550    2670 
   Size:       2550     120
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     379    2187    2296    2670 
   Size:        379    1808     109     374

  Fragments arranged by size: 

                1808     379     374     109
 
BstYI r'GATC_y

Cuts at:      0      11     374     647     905     920    1026    1160    2612 
   Size:         11     363     273     258      15     106     134    1452

Cuts at:   2612    2670 
   Size:         58

  Fragments arranged by size: 

                1452     363     273     258     134     106      58      15
                  11
 
BstZ17I GTA'TAC

Cuts at:      0     201    2670 
   Size:        201    2469
 
BtsI GCAGTG_nn'

Cuts at:      0     860    1714    1827    2098    2670 
   Size:        860     854     113     271     572

  Fragments arranged by size: 

                 860     854     572     271     113
 
ClaI AT'CG_AT

Cuts at:      0     843    2670 
   Size:        843    1827
 
DraIII CAC_nnn'GTG

Cuts at:      0    1219    2670 
   Size:       1219    1451
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     130    2670 
   Size:        130    2540
 
EaeI y'GGCC_r

Cuts at:      0     342    1490    2670 
   Size:        342    1148    1180

  Fragments arranged by size: 

                1180    1148     342
 
EarI CTCTTCn'nnn_

Cuts at:      0     316     718     798    1249    1487    1910    1940    2117 
   Size:        316     402      80     451     238     423      30     177

Cuts at:   2117    2198    2670 
   Size:         81     472

  Fragments arranged by size: 

                 472     451     423     402     316     238     177      81
                  80      30
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      11    2670 
   Size:         11    2659
 
EcoICRI GAG'CTC

Cuts at:      0    1686    2670 
   Size:       1686     984
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     212     340     622    1113    1156    1570    1771    2050 
   Size:        212     128     282     491      43     414     201     279

Cuts at:   2050    2141    2227    2670 
   Size:         91      86     443

  Fragments arranged by size: 

                 491     443     414     282     279     212     201     128
                  91      86      43
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     697     723    2670 
   Size:        697      26    1947

  Fragments arranged by size: 

                1947     697      26
 
EcoO109I rG'GnC_Cy

Cuts at:      0     215    2670 
   Size:        215    2455
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0      31     598    2670 
   Size:         31     567    2072

  Fragments arranged by size: 

                2072     567      31
 
HaeII r_GCGC'y

Cuts at:      0     556    1242    2487    2670 
   Size:        556     686    1245     183

  Fragments arranged by size: 

                1245     686     556     183
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0       3      35      79     111     694     726    1175    1207 
   Size:          3      32      44      32     583      32     449      32

Cuts at:   1207    2670 
   Size:       1463

  Fragments arranged by size: 

                1463     583     449      44      32      32      32      32
                   3
 
HincII GTy'rAC

Cuts at:      0    1391    2670 
   Size:       1391    1279
 
KpnI G_GTAC'C

Cuts at:      0     518     758    2495    2670 
   Size:        518     240    1737     175

  Fragments arranged by size: 

                1737     518     240     175
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     215     613     675     727    2670 
   Size:        215     398      62      52    1943

  Fragments arranged by size: 

                1943     398     215      62      52
 
MscI TGG'CCA

Cuts at:      0     344    1492    2670 
   Size:        344    1148    1178

  Fragments arranged by size: 

                1178    1148     344
 
MslI CAynn'nnrTG

Cuts at:      0    1666    2185    2670 
   Size:       1666     519     485

  Fragments arranged by size: 

                1666     519     485
 
MspA1I CmG'CkG

Cuts at:      0      28      82     825    1316    1749    1806    2670 
   Size:         28      54     743     491     433      57     864

  Fragments arranged by size: 

                 864     743     491     433      57      54      28
 
NdeI CA'TA_TG

Cuts at:      0     474    2670 
   Size:        474    2196
 
NheI G'CTAG_C

Cuts at:      0    1510    2670 
   Size:       1510    1160
 
NsiI A_TGCA'T

Cuts at:      0    1760    2670 
   Size:       1760     910
 
NspI r_CATG'y

Cuts at:      0    1528    2426    2670 
   Size:       1528     898     244

  Fragments arranged by size: 

                1528     898     244
 
PasI CC'CwG_GG

Cuts at:      0    1308    2670 
   Size:       1308    1362
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    2364    2670 
   Size:       2364     306
 
PshAI GACnn'nnGTC

Cuts at:      0    1980    2670 
   Size:       1980     690
 
PspXI vC'TCGA_Gb

Cuts at:      0    1394    2670 
   Size:       1394    1276
 
PstI C_TGCA'G

Cuts at:      0      87    2378    2599    2670 
   Size:         87    2291     221      71

  Fragments arranged by size: 

                2291     221      87      71
 
PvuII CAG'CTG

Cuts at:      0      82     825    1749    2670 
   Size:         82     743     924     921

  Fragments arranged by size: 

                 924     921     743      82
 
SacI G_AGCT'C

Cuts at:      0    1688    2670 
   Size:       1688     982
 
SapI GCTCTTCn'nnn_

Cuts at:      0     316    1940    2670 
   Size:        316    1624     730

  Fragments arranged by size: 

                1624     730     316
 
SexAI A'CCwGG_T

Cuts at:      0    1364    2670 
   Size:       1364    1306
 
SfcI C'TryA_G

Cuts at:      0      83    1744    2374    2595    2670 
   Size:         83    1661     630     221      75

  Fragments arranged by size: 

                1661     630     221      83      75
 
SmlI C'TyrA_G

Cuts at:      0     204    1394    1885    2670 
   Size:        204    1190     491     785

  Fragments arranged by size: 

                1190     785     491     204
 
SphI G_CATG'C

Cuts at:      0    1528    2670 
   Size:       1528    1142
 
StuI AGG'CCT

Cuts at:      0     692    1063    1631    2670 
   Size:        692     371     568    1039

  Fragments arranged by size: 

                1039     692     568     371
 
StyI C'CwwG_G

Cuts at:      0     302     573    1404    2460    2487    2670 
   Size:        302     271     831    1056      27     183

  Fragments arranged by size: 

                1056     831     302     271     183      27
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     315    1126    1852    2071    2670 
   Size:        315     811     726     219     599

  Fragments arranged by size: 

                 811     726     599     315     219
 
TatI w'GTAC_w

Cuts at:      0     441    2670 
   Size:        441    2229
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     860     892    1801    1833    2670 
   Size:        860      32     909      32     837

  Fragments arranged by size: 

                 909     860     837      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0      49    2670 
   Size:         49    2621
 
XhoI C'TCGA_G

Cuts at:      0    1394    2670 
   Size:       1394    1276
 
XmnI GAAnn'nnTTC

Cuts at:      0    1481    1919    2542    2670 
   Size:       1481     438     623     128

  Fragments arranged by size: 

                1481     623     438     128
 

 Enzymes that do cut:

     AccI   Acc65I     AcuI     AfeI     AgeI     AlfI    AlwNI     ApoI
     AvaI    BamHI     BanI    BanII     BbsI    BbvCI     BcgI    BciVI
    BfrBI     BglI    BglII     BlpI Bme1580I     BmrI     BmtI     BplI
     BpmI   Bpu10I    BpuEI     BsaI    BsaBI    BsaWI    BsaXI    BseRI
    BseYI     BsgI  BsiHKAI    BsmBI Bsp1286I    BspMI    BsrBI    BsrDI
    BsrFI   BstAPI    BstBI    BstXI    BstYI  BstZ17I     BtsI     ClaI
   DraIII     DrdI     EaeI     EarI     EciI  EcoICRI  Eco57MI    EcoNI
 EcoO109I     FalI    HaeII    Hin4I   HincII     KpnI     MmeI     MscI
     MslI   MspA1I     NdeI     NheI     NsiI     NspI     PasI    PflMI
    PshAI    PspXI     PstI    PvuII     SacI     SapI    SexAI     SfcI
     SmlI     SphI     StuI     StyI    TaqII     TatI     TstI  Tth111I
     XhoI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AclI    AflII   AflIII     AhdI     AleI     AloI
     ApaI    ApaLI     AscI     AseI    AsiSI    AvrII     BaeI     BbeI
     BclI    BmgBI    BsaAI    BsaHI    BsiEI    BsiWI     BsmI    BspEI
    BspHI    BsrGI   BssHII    BssSI   BstEII   Bsu36I     BtgI    BtgZI
    CspCI     DraI     EagI    EcoRI    EcoRV     FseI     FspI    FspAI
  HindIII     HpaI     KasI     MfeI     MluI     NaeI     NarI     NcoI
   NgoMIV     NotI     NruI     PacI     PciI     PfoI     PmeI     PmlI
     PpiI    PpuMI     PsiI   PspOMI     PsrI     PvuI    RsrII    SacII
     SalI    SanDI     SbfI     ScaI     SfiI     SfoI     SgfI    SgrAI
     SmaI    SnaBI     SpeI     SrfI     SspI     SwaI     XbaI     XcmI
     XmaI     ZraI
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