Restriction map for
pF1KB8431
(Circular) (Six-base) MAPSORT of: pF1KB8431.seq Check: 772 from: 1 to: 5000
pF1KB8431 5000 bp
With 182 enzymes: SgfI *
March 2, 2010 14:28 ..
AccI GT'mk_AC
Cuts at: 1324 2038 1324
Size: 714 4286
Acc65I G'GTAC_C
Cuts at: 2016 2016
Size: 5000
AclI AA'CG_TT
Cuts at: 4840 4840
Size: 5000
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 146 218 283 2825 3257 4004 4272 146
Size: 72 65 2542 432 747 268 874
Fragments arranged by size:
2542 874 747 432 268 72 65
AflIII A'CryG_T
Cuts at: 1932 2352 3456 4433 1932
Size: 420 1104 977 2499
Fragments arranged by size:
2499 1104 977 420
AgeI A'CCGG_T
Cuts at: 3388 3388
Size: 5000
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1656 1656
Size: 5000
AlwNI CAG_nnn'CTG
Cuts at: 2305 3872 2305
Size: 1567 3433
ApaLI G'TGCA_C
Cuts at: 3770 3770
Size: 5000
ApoI r'AATT_y
Cuts at: 184 369 493 549 577 900 1196 1315 1458
Size: 185 124 56 28 323 296 119 143
Cuts at: 1458 2004 4353 4609 184
Size: 546 2349 256 575
Fragments arranged by size:
2349 575 546 323 296 256 185 143
124 119 56 28
AseI AT'TA_AT
Cuts at: 20 335 20
Size: 315 4685
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5000
AvaI C'yCGr_G
Cuts at: 524 2020 524
Size: 1496 3504
BamHI G'GATC_C
Cuts at: 2025 2025
Size: 5000
BanI G'GyrC_C
Cuts at: 2016 2430 2678 2713 2016
Size: 414 248 35 4303
Fragments arranged by size:
4303 414 248 35
BanII G_rGCy'C
Cuts at: 2014 3044 2014
Size: 1030 3970
BbeI G_GCGC'C
Cuts at: 2434 2682 2434
Size: 248 4752
BbsI GAAGACnn'nnnn_
Cuts at: 237 1363 237
Size: 1126 3874
BbvCI CC'TCA_GC
Cuts at: 1234 1234
Size: 5000
BciVI GTATCCnnnnn_n'
Cuts at: 2898 3659 5000 2898
Size: 761 1341 2898
Fragments arranged by size:
2898 1341 761
BclI T'GATC_A
Cuts at: 2520 2520
Size: 5000
BglII A'GATC_T
Cuts at: 2515 4552 2515
Size: 2037 2963
BlpI GC'TnA_GC
Cuts at: 2112 2112
Size: 5000
Bme1580I G_kGCm'C
Cuts at: 2625 2718 3774 2625
Size: 93 1056 3851
Fragments arranged by size:
3851 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 2212 2293 2628 4772 2212
Size: 81 335 2144 2440
Fragments arranged by size:
2440 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3367 3367
Size: 5000
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 126 1612 126
Size: 1486 3514
Bpu10I CC'TnA_GC
Cuts at: 1234 1234
Size: 5000
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 616 1084 1711 2176 3055 3547 3845 616
Size: 468 627 465 879 492 298 1771
Fragments arranged by size:
1771 879 627 492 468 465 298
BsaI GGTCTCn'nnnn_
Cuts at: 4704 4704
Size: 5000
BsaAI yAC'GTr
Cuts at: 2353 2353
Size: 5000
BsaBI GATnn'nnATC
Cuts at: 6 1508 2514 4551 6
Size: 1502 1006 2037 455
Fragments arranged by size:
2037 1502 1006 455
BsaHI Gr'CG_yC
Cuts at: 2431 2679 3381 4877 2431
Size: 248 702 1496 2554
Fragments arranged by size:
2554 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2189 2710 3388 3662 3809 4220 2189
Size: 521 678 274 147 411 2969
Fragments arranged by size:
2969 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 341 341
Size: 5000
BseYI C'CCAG_C
Cuts at: 2425 3183 3760 4256 2425
Size: 758 577 496 3169
Fragments arranged by size:
3169 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 2542 2588 3796 104
Size: 2438 46 1208 1308
Fragments arranged by size:
2438 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 2014 2792 2982 3774 2014
Size: 778 190 792 3240
Fragments arranged by size:
3240 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 2014 2625 2718 2792 2982 3044 3774 2014
Size: 611 93 74 190 62 730 3240
Fragments arranged by size:
3240 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 4220 4220
Size: 5000
BspHI T'CATG_A
Cuts at: 4176 4176
Size: 5000
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2050 2264 2566 2947 2050
Size: 214 302 381 4103
Fragments arranged by size:
4103 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3292 3346 4830 4995 50
Size: 3242 54 1484 165 55
Fragments arranged by size:
3242 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 631 1851 2912 631
Size: 1220 1061 2719
Fragments arranged by size:
2719 1220 1061
BsrFI r'CCGG_y
Cuts at: 2998 3179 3388 2998
Size: 181 209 4610
Fragments arranged by size:
4610 209 181
BssSI C'ACGA_G
Cuts at: 3271 3629 3271
Size: 358 4642
BstAPI GCAn_nnn'nTGC
Cuts at: 924 2377 924
Size: 1453 3547
BstBI TT'CG_AA
Cuts at: 3361 3361
Size: 5000
BstEII G'GTnAC_C
Cuts at: 871 871
Size: 5000
BstXI CCAn_nnnn'nTGG
Cuts at: 1067 1740 1067
Size: 673 4327
BstYI r'GATC_y
Cuts at: 1 1539 2025 2492 2515 2850 3096 4108 4194
Size: 1538 486 467 23 335 246 1012 86
Cuts at: 4194 4206 4552 1
Size: 12 346 449
Fragments arranged by size:
1538 1012 486 467 449 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 857 1007 107
Size: 750 150 4100
Fragments arranged by size:
4100 750 150
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 143 2950 3131 3294 143
Size: 2807 181 163 1849
Fragments arranged by size:
2807 1849 181 163
BtsI GCAGTG_nn'
Cuts at: 2243 2243
Size: 5000
DraI TTT'AAA
Cuts at: 1999 1999
Size: 5000
DrdI GACnn_nn'nnGTC
Cuts at: 2706 3564 2706
Size: 858 4142
EaeI y'GGCC_r
Cuts at: 2585 2759 3150 3177 4522 2585
Size: 174 391 27 1345 3063
Fragments arranged by size:
3063 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2585 2585
Size: 5000
EarI CTCTTCn'nnn_
Cuts at: 194 302 3023 3233 194
Size: 108 2721 210 1961
Fragments arranged by size:
2721 1961 210 108
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3208 3518 3664 4810 3208
Size: 310 146 1146 3398
Fragments arranged by size:
3398 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2012 2012
Size: 5000
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 126 146 218 283 1612 2825 3257 4004 4272
Size: 20 72 65 1329 1213 432 747 268
Cuts at: 4272 126
Size: 854
Fragments arranged by size:
1329 1213 854 747 432 268 72 65
20
EcoNI CCTnn'n_nnAGG
Cuts at: 1309 1309
Size: 5000
EcoO109I rG'GnC_Cy
Cuts at: 704 717 1189 1906 2139 704
Size: 13 472 717 233 3565
Fragments arranged by size:
3565 717 472 233 13
EcoRI G'AATT_C
Cuts at: 2004 2004
Size: 5000
EcoRV GAT'ATC
Cuts at: 1626 1626
Size: 5000
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 301 1288 3019 301
Size: 987 1731 2282
Fragments arranged by size:
2282 1731 987
FspI TGC'GCA
Cuts at: 2781 2781
Size: 5000
HaeII r_GCGC'y
Cuts at: 2434 2682 3704 4683 2434
Size: 248 1022 979 2751
Fragments arranged by size:
2751 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2842 2872 2874 2904 2842
Size: 30 2 30 4938
Fragments arranged by size:
4938 30 30 2
HincII GTy'rAC
Cuts at: 660 890 1598 1930 2039 660
Size: 230 708 332 109 3621
Fragments arranged by size:
3621 708 332 230 109
HpaI GTT'AAC
Cuts at: 890 890
Size: 5000
KasI G'GCGC_C
Cuts at: 2430 2678 2430
Size: 248 4752
KpnI G_GTAC'C
Cuts at: 2020 2020
Size: 5000
MluI A'CGCG_T
Cuts at: 4433 4433
Size: 5000
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3671 3855 3671
Size: 184 4816
MscI TGG'CCA
Cuts at: 2761 2761
Size: 5000
MslI CAynn'nnrTG
Cuts at: 2989 3116 2989
Size: 127 4873
MspA1I CmG'CkG
Cuts at: 2111 2425 2785 3798 4043 2111
Size: 314 360 1013 245 3068
Fragments arranged by size:
3068 1013 360 314 245
NaeI GCC'GGC
Cuts at: 3181 3181
Size: 5000
NarI GG'CG_CC
Cuts at: 2431 2679 2431
Size: 248 4752
NcoI C'CATG_G
Cuts at: 107 857 1007 107
Size: 750 150 4100
Fragments arranged by size:
4100 750 150
NdeI CA'TA_TG
Cuts at: 1667 1667
Size: 5000
NgoMIV G'CCGG_C
Cuts at: 3179 3179
Size: 5000
NspI r_CATG'y
Cuts at: 307 927 1610 1679 1936 2053 2553 3460 307
Size: 620 683 69 257 117 500 907 1847
Fragments arranged by size:
1847 907 683 620 500 257 117 69
PciI A'CATG_T
Cuts at: 1932 3456 1932
Size: 1524 3476
PmeI GTTT'AAAC
Cuts at: 1999 1999
Size: 5000
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4139 4171 4139
Size: 32 4968
PpuMI rG'GwC_Cy
Cuts at: 704 717 1189 1906 704
Size: 13 472 717 3798
Fragments arranged by size:
3798 717 472 13
PsiI TTA'TAA
Cuts at: 491 491
Size: 5000
PstI C_TGCA'G
Cuts at: 1453 2047 2732 1453
Size: 594 685 3721
Fragments arranged by size:
3721 685 594
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5000
PvuII CAG'CTG
Cuts at: 2425 2785 2425
Size: 360 4640
RsrII CG'GwC_CG
Cuts at: 3195 3195
Size: 5000
SacI G_AGCT'C
Cuts at: 2014 2014
Size: 5000
SalI G'TCGA_C
Cuts at: 2037 2037
Size: 5000
SanDI GG'GwC_CC
Cuts at: 704 717 1906 704
Size: 13 1189 3798
Fragments arranged by size:
3798 1189 13
SapI GCTCTTCn'nnn_
Cuts at: 302 3023 3233 302
Size: 2721 210 2069
Fragments arranged by size:
2721 2069 210
SbfI CC_TGCA'GG
Cuts at: 2047 2047
Size: 5000
ScaI AGT'ACT
Cuts at: 4517 4517
Size: 5000
SfcI C'TryA_G
Cuts at: 33 1126 1449 2043 2728 3721 3912 33
Size: 1093 323 594 685 993 191 1121
Fragments arranged by size:
1121 1093 993 685 594 323 191
SfoI GGC'GCC
Cuts at: 2432 2680 2432
Size: 248 4752
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5000
SmaI CCC'GGG
Cuts at: 2022 2022
Size: 5000
SmlI C'TyrA_G
Cuts at: 524 631 1099 1690 2155 3070 3562 3824 524
Size: 107 468 591 465 915 492 262 1700
Fragments arranged by size:
1700 915 591 492 468 465 262 107
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5000
SphI G_CATG'C
Cuts at: 307 2053 2553 307
Size: 1746 500 2754
Fragments arranged by size:
2754 1746 500
SspI AAT'ATT
Cuts at: 209 209
Size: 5000
StyI C'CwwG_G
Cuts at: 107 857 1007 1066 2134 2488 107
Size: 750 150 59 1068 354 2619
Fragments arranged by size:
2619 1068 750 354 150 59
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2583 2716 3175 3384 2583
Size: 133 459 209 4199
Fragments arranged by size:
4199 459 209 133
TatI w'GTAC_w
Cuts at: 980 4515 980
Size: 3535 1465
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4517 4549 4517
Size: 32 4968
Tth111I GACn'n_nGTC
Cuts at: 2797 2797
Size: 5000
XbaI T'CTAG_A
Cuts at: 2031 2031
Size: 5000
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 549 1917 2495 549
Size: 1368 578 3054
Fragments arranged by size:
3054 1368 578
XhoI C'TCGA_G
Cuts at: 524 524
Size: 5000
XmaI C'CCGG_G
Cuts at: 2020 2020
Size: 5000
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstEII BstXI BstYI BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HpaI
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NspI PciI PmeI PpiI
PpuMI PsiI PstI PvuI PvuII RsrII SacI SalI
SanDI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI SspI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI ApaI
AscI AvrII BaeI BcgI BfrBI BglI BmgBI BmtI
BsaXI BsgI BsiWI BsmI BsmBI BsrGI BssHII BstZ17I
Bsu36I ClaI CspCI DraIII FseI FspAI HindIII MfeI
NheI NotI NruI NsiI PacI PasI PflMI PfoI
PmlI PshAI PspOMI PspXI PsrI SacII SexAI SfiI
SgrAI SnaBI SrfI StuI SwaI XmnI ZraI