Restriction map for pF1KA0746
 (Linear) (Six-base) MAPSORT of: KIAA0746.seq  Check: 887  from: 1  to: 2937



KIAA0746.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:00  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     814    2637    2937 
   Size:        814    1823     300

  Fragments arranged by size: 

                1823     814     300
 
AflIII A'CryG_T

Cuts at:      0    2136    2937 
   Size:       2136     801
 
AleI CACnn'nnGTG

Cuts at:      0    2285    2937 
   Size:       2285     652
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1280    1312    2937 
   Size:       1280      32    1625

  Fragments arranged by size: 

                1625    1280      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0      95    1238    1635    2248    2613    2660    2842    2937 
   Size:         95    1143     397     613     365      47     182      95

  Fragments arranged by size: 

                1143     613     397     365     182      95      95      47
            
 
ApoI r'AATT_y

Cuts at:      0     580    1864    2271    2937 
   Size:        580    1284     407     666

  Fragments arranged by size: 

                1284     666     580     407
 
AvaI C'yCGr_G

Cuts at:      0     342    1301    1595    2937 
   Size:        342     959     294    1342

  Fragments arranged by size: 

                1342     959     342     294
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0    2307    2340    2937 
   Size:       2307      33     597

  Fragments arranged by size: 

                2307     597      33
 
BamHI G'GATC_C

Cuts at:      0    1716    2937 
   Size:       1716    1221
 
BanI G'GyrC_C

Cuts at:      0    1699    2937 
   Size:       1699    1238
 
BbeI G_GCGC'C

Cuts at:      0    1703    2937 
   Size:       1703    1234
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     566    1183    1355    2937 
   Size:        566     617     172    1582

  Fragments arranged by size: 

                1582     617     566     172
 
BbvCI CC'TCA_GC

Cuts at:      0     896    2937 
   Size:        896    2041
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1237    2937 
   Size:       1237    1700
 
BfrBI ATG'CAT

Cuts at:      0     543     925    2937 
   Size:        543     382    2012

  Fragments arranged by size: 

                2012     543     382
 
BlpI GC'TnA_GC

Cuts at:      0    1613    2937 
   Size:       1613    1324
 
BmgBI CAC'GTC

Cuts at:      0    2849    2937 
   Size:       2849      88
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     391    1428    2937 
   Size:        391    1037    1509

  Fragments arranged by size: 

                1509    1037     391
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     291     385     976    1105    1726    1997    2110    2464 
   Size:        291      94     591     129     621     271     113     354

Cuts at:   2464    2590    2649    2858    2937 
   Size:        126      59     209      79

  Fragments arranged by size: 

                 621     591     354     291     271     209     129     126
                 113      94      79      59
 
Bpu10I CC'TnA_GC

Cuts at:      0     896    2937 
   Size:        896    2041
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     226     868    1522    2937 
   Size:        226     642     654    1415

  Fragments arranged by size: 

                1415     654     642     226
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1002    2396    2937 
   Size:       1002    1394     541

  Fragments arranged by size: 

                1394    1002     541
 
BsaAI yAC'GTr

Cuts at:      0    2932    2937 
   Size:       2932       5
 
BsaHI Gr'CG_yC

Cuts at:      0     378    1700    2937 
   Size:        378    1322    1237

  Fragments arranged by size: 

                1322    1237     378
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    2622    2652    2937 
   Size:       2622      30     285

  Fragments arranged by size: 

                2622     285      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    2305    2659    2937 
   Size:       2305     354     278

  Fragments arranged by size: 

                2305     354     278
 
BseYI C'CCAG_C

Cuts at:      0     730    1063    2937 
   Size:        730     333    1874

  Fragments arranged by size: 

                1874     730     333
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     802    1041    2476    2668    2698    2797    2937 
   Size:        802     239    1435     192      30      99     140

  Fragments arranged by size: 

                1435     802     239     192     140      99      30
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1702    2937 
   Size:       1702    1235
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     746     989    2690    2937 
   Size:        746     243    1701     247

  Fragments arranged by size: 

                1701     746     247     243
 
BsrDI GCAATG_nn'

Cuts at:      0    1078    1448    1608    2937 
   Size:       1078     370     160    1329

  Fragments arranged by size: 

                1329    1078     370     160
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1482    2937 
   Size:       1482    1455
 
BstYI r'GATC_y

Cuts at:      0    1092    1716    2106    2937 
   Size:       1092     624     390     831

  Fragments arranged by size: 

                1092     831     624     390
 
BtsI GCAGTG_nn'

Cuts at:      0      56    1480    2840    2852    2937 
   Size:         56    1424    1360      12      85

  Fragments arranged by size: 

                1424    1360      85      56      12
 
ClaI AT'CG_AT

Cuts at:      0    2369    2937 
   Size:       2369     568
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0      66     101     435     470     639     674    2937 
   Size:         66      35     334      35     169      35    2263

  Fragments arranged by size: 

                2263     334     169      66      35      35      35
 
EaeI y'GGCC_r

Cuts at:      0    1658    2306    2418    2937 
   Size:       1658     648     112     519

  Fragments arranged by size: 

                1658     648     519     112
 
EarI CTCTTCn'nnn_

Cuts at:      0     512    2937 
   Size:        512    2425
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     291     385     814     976    1105    1726    1997    2110 
   Size:        291      94     429     162     129     621     271     113

Cuts at:   2110    2464    2590    2637    2649    2858    2937 
   Size:        354     126      47      12     209      79

  Fragments arranged by size: 

                 621     429     354     291     271     209     162     129
                 126     113      94      79      47      12
 
EcoO109I rG'GnC_Cy

Cuts at:      0    2051    2937 
   Size:       2051     886
 
EcoRV GAT'ATC

Cuts at:      0     618    2937 
   Size:        618    2319
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     220    2937 
   Size:        220    2717
 
FspI TGC'GCA

Cuts at:      0    2030    2937 
   Size:       2030     907
 
HaeII r_GCGC'y

Cuts at:      0    1703    2937 
   Size:       1703    1234
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      20      52     602     634     822     854    1345    1377 
   Size:         20      32     550      32     188      32     491      32

Cuts at:   1377    1701    1733    2473    2505    2937 
   Size:        324      32     740      32     432

  Fragments arranged by size: 

                 740     550     491     432     324     188      32      32
                  32      32      32      32      20
 
HincII GTy'rAC

Cuts at:      0     474    2328    2937 
   Size:        474    1854     609

  Fragments arranged by size: 

                1854     609     474
 
HindIII A'AGCT_T

Cuts at:      0    2216    2937 
   Size:       2216     721
 
HpaI GTT'AAC

Cuts at:      0    2328    2937 
   Size:       2328     609
 
KasI G'GCGC_C

Cuts at:      0    1699    2937 
   Size:       1699    1238
 
MfeI C'AATT_G

Cuts at:      0    1679    2937 
   Size:       1679    1258
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1317    2937 
   Size:       1317    1620
 
MscI TGG'CCA

Cuts at:      0    1660    2308    2420    2937 
   Size:       1660     648     112     517

  Fragments arranged by size: 

                1660     648     517     112
 
MslI CAynn'nnrTG

Cuts at:      0     719    1961    2285    2937 
   Size:        719    1242     324     652

  Fragments arranged by size: 

                1242     719     652     324
 
MspA1I CmG'CkG

Cuts at:      0     730    1235    1311    2866    2878    2937 
   Size:        730     505      76    1555      12      59

  Fragments arranged by size: 

                1555     730     505      76      59      12
 
NarI GG'CG_CC

Cuts at:      0    1700    2937 
   Size:       1700    1237
 
NdeI CA'TA_TG

Cuts at:      0    1500    2937 
   Size:       1500    1437
 
NsiI A_TGCA'T

Cuts at:      0     545     927    2937 
   Size:        545     382    2010

  Fragments arranged by size: 

                2010     545     382
 
NspI r_CATG'y

Cuts at:      0     965    1323    2140    2937 
   Size:        965     358     817     797

  Fragments arranged by size: 

                 965     817     797     358
 
PasI CC'CwG_GG

Cuts at:      0    1034    2493    2937 
   Size:       1034    1459     444

  Fragments arranged by size: 

                1459    1034     444
 
PciI A'CATG_T

Cuts at:      0    2136    2937 
   Size:       2136     801
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     387    2553    2937 
   Size:        387    2166     384

  Fragments arranged by size: 

                2166     387     384
 
PfoI T'CCnGG_A

Cuts at:      0     701    1983    2606    2937 
   Size:        701    1282     623     331

  Fragments arranged by size: 

                1282     701     623     331
 
PmlI CAC'GTG

Cuts at:      0    2932    2937 
   Size:       2932       5
 
PpuMI rG'GwC_Cy

Cuts at:      0    2051    2937 
   Size:       2051     886
 
PsiI TTA'TAA

Cuts at:      0     719    2937 
   Size:        719    2218
 
PstI C_TGCA'G

Cuts at:      0      51     158     933    1316    2883    2937 
   Size:         51     107     775     383    1567      54

  Fragments arranged by size: 

                1567     775     383     107      54      51
 
PvuII CAG'CTG

Cuts at:      0     730    1235    1311    2866    2878    2937 
   Size:        730     505      76    1555      12      59

  Fragments arranged by size: 

                1555     730     505      76      59      12
 
ScaI AGT'ACT

Cuts at:      0     799    1897    2937 
   Size:        799    1098    1040

  Fragments arranged by size: 

                1098    1040     799
 
SfcI C'TryA_G

Cuts at:      0      47     131     154     929    1312    1440    2879    2937 
   Size:         47      84      23     775     383     128    1439      58

  Fragments arranged by size: 

                1439     775     383     128      84      58      47      23
            
 
SfoI GGC'GCC

Cuts at:      0    1701    2937 
   Size:       1701    1236
 
SmlI C'TyrA_G

Cuts at:      0     205     847    1537    2937 
   Size:        205     642     690    1400

  Fragments arranged by size: 

                1400     690     642     205
 
SphI G_CATG'C

Cuts at:      0     965    2937 
   Size:        965    1972
 
SspI AAT'ATT

Cuts at:      0    2014    2937 
   Size:       2014     923
 
StuI AGG'CCT

Cuts at:      0    2183    2830    2937 
   Size:       2183     647     107

  Fragments arranged by size: 

                2183     647     107
 
StyI C'CwwG_G

Cuts at:      0    1108    1694    1817    2666    2937 
   Size:       1108     586     123     849     271

  Fragments arranged by size: 

                1108     849     586     271     123
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    2311    2937 
   Size:       2311     626
 
TatI w'GTAC_w

Cuts at:      0     464     797    1895    2937 
   Size:        464     333    1098    1042

  Fragments arranged by size: 

                1098    1042     464     333
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    2243    2275    2844    2876    2937 
   Size:       2243      32     569      32      61

  Fragments arranged by size: 

                2243     569      61      32      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1000    1701    2316    2937 
   Size:       1000     701     615     621

  Fragments arranged by size: 

                1000     701     621     615
 

 Enzymes that do cut:

     AcuI   AflIII     AleI     AloI    AlwNI     ApoI     AvaI     BaeI
    BamHI     BanI     BbeI     BbsI    BbvCI    BciVI    BfrBI     BlpI
    BmgBI     BmrI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaHI
    BsaXI    BseRI    BseYI     BsgI    BsmBI    BspMI    BsrDI   BstAPI
    BstYI     BtsI     ClaI    CspCI     EaeI     EarI  Eco57MI EcoO109I
    EcoRV     FalI     FspI    HaeII    Hin4I   HincII  HindIII     HpaI
     KasI     MfeI     MmeI     MscI     MslI   MspA1I     NarI     NdeI
     NsiI     NspI     PasI     PciI    PflMI     PfoI     PmlI    PpuMI
     PsiI     PstI    PvuII     ScaI     SfcI     SfoI     SmlI     SphI
     SspI     StuI     StyI    TaqII     TatI     TstI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI
     AhdI     AlfI     ApaI    ApaLI     AscI     AseI    AsiSI    AvrII
    BanII     BcgI     BclI     BglI    BglII Bme1580I     BmtI     BplI
    BsaBI    BsaWI    BsiEI  BsiHKAI    BsiWI     BsmI Bsp1286I    BspEI
    BspHI    BsrBI    BsrFI    BsrGI   BssHII    BssSI    BstBI   BstEII
    BstXI  BstZ17I   Bsu36I     BtgI    BtgZI     DraI   DraIII     DrdI
     EagI     EciI  EcoICRI    EcoNI    EcoRI     FseI    FspAI     KpnI
     MluI     NaeI     NcoI   NgoMIV     NheI     NotI     NruI     PacI
     PmeI     PpiI    PshAI   PspOMI    PspXI     PsrI     PvuI    RsrII
     SacI    SacII     SalI    SanDI     SapI     SbfI    SexAI     SfiI
     SgfI    SgrAI     SmaI    SnaBI     SpeI     SrfI     SwaI  Tth111I
     XbaI     XhoI     XmaI     XmnI     ZraI
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