Restriction map for pF1KB7330
 (Linear) (Six-base) MAPSORT of: KIBB7330.seq  Check: 2539  from: 1  to: 2616



KIBB7330.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:07  ..

AatII G_ACGT'C

Cuts at:      0    1700    2616 
   Size:       1700     916
 
AccI GT'mk_AC

Cuts at:      0      29    2039    2616 
   Size:         29    2010     577

  Fragments arranged by size: 

                2010     577      29
 
Acc65I G'GTAC_C

Cuts at:      0     458    2616 
   Size:        458    2158
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      79     973    2388    2397    2616 
   Size:         79     894    1415       9     219

  Fragments arranged by size: 

                1415     894     219      79       9
 
AflIII A'CryG_T

Cuts at:      0     293    2354    2616 
   Size:        293    2061     262

  Fragments arranged by size: 

                2061     293     262
 
AhdI GACnn_n'nnGTC

Cuts at:      0    1734    2616 
   Size:       1734     882
 
AleI CACnn'nnGTG

Cuts at:      0     804    2616 
   Size:        804    1812
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     465    1081    2616 
   Size:        465     616    1535

  Fragments arranged by size: 

                1535     616     465
 
AlwNI CAG_nnn'CTG

Cuts at:      0     615    2616 
   Size:        615    2001
 
ApaI G_GGCC'C

Cuts at:      0     375    1271    1789    1822    2552    2609    2616 
   Size:        375     896     518      33     730      57       7

  Fragments arranged by size: 

                 896     730     518     375      57      33       7
 
ApoI r'AATT_y

Cuts at:      0     234    2616 
   Size:        234    2382
 
AscI GG'CGCG_CC

Cuts at:      0      89    2616 
   Size:         89    2527
 
AvaI C'yCGr_G

Cuts at:      0      39     104    1340    1417    1599    1840    1867    2313 
   Size:         39      65    1236      77     182     241      27     446

Cuts at:   2313    2329    2616 
   Size:         16     287

  Fragments arranged by size: 

                1236     446     287     241     182      77      65      39
                  27      16
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0    2520    2553    2616 
   Size:       2520      33      63

  Fragments arranged by size: 

                2520      63      33
 
BanI G'GyrC_C

Cuts at:      0     187     458     646    1437    1498    1998    2106    2185 
   Size:        187     271     188     791      61     500     108      79

Cuts at:   2185    2472    2616 
   Size:        287     144

  Fragments arranged by size: 

                 791     500     287     271     188     187     144     108
                  79      61
 
BanII G_rGCy'C

Cuts at:      0     364     375     667    1271    1789    1822    2552    2609 
   Size:        364      11     292     604     518      33     730      57

Cuts at:   2609    2616 
   Size:          7

  Fragments arranged by size: 

                 730     604     518     364     292      57      33      11
                   7
 
BbeI G_GCGC'C

Cuts at:      0     191    2002    2110    2476    2616 
   Size:        191    1811     108     366     140

  Fragments arranged by size: 

                1811     366     191     140     108
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1320    2027    2616 
   Size:       1320     707     589

  Fragments arranged by size: 

                1320     707     589
 
BbvCI CC'TCA_GC

Cuts at:      0     160    1180    2616 
   Size:        160    1020    1436

  Fragments arranged by size: 

                1436    1020     160
 
BclI T'GATC_A

Cuts at:      0     815    2616 
   Size:        815    1801
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1343    2616 
   Size:       1343    1273
 
Bme1580I G_kGCm'C

Cuts at:      0     375    1109    1271    1442    1476    1789    1822    2552 
   Size:        375     734     162     171      34     313      33     730

Cuts at:   2552    2609    2616 
   Size:         57       7

  Fragments arranged by size: 

                 734     730     375     313     171     162      57      34
                  33       7
 
BmgBI CAC'GTC

Cuts at:      0    1689    2357    2592    2616 
   Size:       1689     668     235      24

  Fragments arranged by size: 

                1689     668     235      24
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    2418    2616 
   Size:       2418     198
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     378    1728    2616 
   Size:        378    1350     888

  Fragments arranged by size: 

                1350     888     378
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     274     489    1050    1128    1192    1317    1470    2058 
   Size:        274     215     561      78      64     125     153     588

Cuts at:   2058    2413    2616 
   Size:        355     203

  Fragments arranged by size: 

                 588     561     355     274     215     203     153     125
                  78      64
 
Bpu10I CC'TnA_GC

Cuts at:      0     160    1180    2616 
   Size:        160    1020    1436

  Fragments arranged by size: 

                1436    1020     160
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     466    1174    2616 
   Size:        466     708    1442

  Fragments arranged by size: 

                1442     708     466
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1954    2616 
   Size:       1954     662
 
BsaAI yAC'GTr

Cuts at:      0     294    1076    2616 
   Size:        294     782    1540

  Fragments arranged by size: 

                1540     782     294
 
BsaBI GATnn'nnATC

Cuts at:      0     514    1945    2616 
   Size:        514    1431     671

  Fragments arranged by size: 

                1431     671     514
 
BsaHI Gr'CG_yC

Cuts at:      0     188    1697    1999    2107    2473    2616 
   Size:        188    1509     302     108     366     143

  Fragments arranged by size: 

                1509     366     302     188     143     108
 
BsaWI w'CCGG_w

Cuts at:      0     590    2616 
   Size:        590    2026
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1054    1084    1126    1156    2173    2194    2203    2224 
   Size:       1054      30      42      30    1017      21       9      21

Cuts at:   2224    2497    2509    2527    2539    2616 
   Size:        273      12      18      12      77

  Fragments arranged by size: 

                1054    1017     273      77      42      30      30      21
                  21      18      12      12       9
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    2401    2616 
   Size:       2401     215
 
BseYI C'CCAG_C

Cuts at:      0     269     660    1399    1429    1788    1849    2597    2616 
   Size:        269     391     739      30     359      61     748      19

  Fragments arranged by size: 

                 748     739     391     359     269      61      30      19
            
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      25    1051    1281    2616 
   Size:         25    1026     230    1335

  Fragments arranged by size: 

                1335    1026     230      25
 
BsiEI CG_ry'CG

Cuts at:      0     262    1826    2117    2251    2468    2616 
   Size:        262    1564     291     134     217     148

  Fragments arranged by size: 

                1564     291     262     217     148     134
 
BsiHKAI G_wGCw'C

Cuts at:      0     364     527    2616 
   Size:        364     163    2089

  Fragments arranged by size: 

                2089     364     163
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1244    2616 
   Size:       1244    1372
 
Bsp1286I G_dGCh'C

Cuts at:      0     364     375     527     667    1109    1271    1442    1476 
   Size:        364      11     152     140     442     162     171      34

Cuts at:   1476    1789    1822    2552    2609    2616 
   Size:        313      33     730      57       7

  Fragments arranged by size: 

                 730     442     364     313     171     162     152     140
                  57      34      33      11       7
 
BspEI T'CCGG_A

Cuts at:      0     590    2616 
   Size:        590    2026
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1073    1875    2348    2616 
   Size:       1073     802     473     268

  Fragments arranged by size: 

                1073     802     473     268
 
BsrBI CCG'CTC

Cuts at:      0      44     258    2247    2616 
   Size:         44     214    1989     369

  Fragments arranged by size: 

                1989     369     214      44
 
BsrDI GCAATG_nn'

Cuts at:      0     536    2498    2616 
   Size:        536    1962     118

  Fragments arranged by size: 

                1962     536     118
 
BsrFI r'CCGG_y

Cuts at:      0     127     455     553     635    1717    1995    2569    2616 
   Size:        127     328      98      82    1082     278     574      47

  Fragments arranged by size: 

                1082     574     328     278     127      98      82      47
            
 
BsrGI T'GTAC_A

Cuts at:      0    1112    2616 
   Size:       1112    1504
 
BssHII G'CGCG_C

Cuts at:      0      89    2616 
   Size:         89    2527
 
BssSI C'ACGA_G

Cuts at:      0     319    2242    2383    2616 
   Size:        319    1923     141     233

  Fragments arranged by size: 

                1923     319     233     141
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     615    1259    2616 
   Size:        615     644    1357

  Fragments arranged by size: 

                1357     644     615
 
BstEII G'GTnAC_C

Cuts at:      0    1098    1394    2616 
   Size:       1098     296    1222

  Fragments arranged by size: 

                1222    1098     296
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1857    2616 
   Size:       1857     759
 
BstYI r'GATC_y

Cuts at:      0     431    2616 
   Size:        431    2185
 
BstZ17I GTA'TAC

Cuts at:      0      30    2616 
   Size:         30    2586
 
BtgI C'CryG_G

Cuts at:      0     462    1895    2084    2181    2195    2205    2375    2468 
   Size:        462    1433     189      97      14      10     170      93

Cuts at:   2468    2616 
   Size:        148

  Fragments arranged by size: 

                1433     462     189     170     148      97      93      14
                  10
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     513    2616 
   Size:        513    2103
 
BtsI GCAGTG_nn'

Cuts at:      0     698    2616 
   Size:        698    1918
 
EaeI y'GGCC_r

Cuts at:      0     259     548    1719    1823    2114    2465    2564    2616 
   Size:        259     289    1171     104     291     351      99      52

  Fragments arranged by size: 

                1171     351     291     289     259     104      99      52
            
 
EagI C'GGCC_G

Cuts at:      0     259    1823    2114    2465    2616 
   Size:        259    1564     291     351     151

  Fragments arranged by size: 

                1564     351     291     259     151
 
EarI CTCTTCn'nnn_

Cuts at:      0     352     985    2616 
   Size:        352     633    1631

  Fragments arranged by size: 

                1631     633     352
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    1011    1846    2126    2247    2616 
   Size:       1011     835     280     121     369

  Fragments arranged by size: 

                1011     835     369     280     121
 
EcoICRI GAG'CTC

Cuts at:      0     362    2616 
   Size:        362    2254
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      79     274     489     973    1050    1128    1192    1317 
   Size:         79     195     215     484      77      78      64     125

Cuts at:   1317    1470    2058    2388    2397    2413    2616 
   Size:        153     588     330       9      16     203

  Fragments arranged by size: 

                 588     484     330     215     203     195     153     125
                  79      78      77      64      16       9
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    2299    2616 
   Size:       2299     317
 
EcoO109I rG'GnC_Cy

Cuts at:      0     138     371     372     810    1139    1221    1267    1268 
   Size:        138     233       1     438     329      82      46       1

Cuts at:   1268    1367    1590    1785    1818    1845    2324    2492    2549 
   Size:         99     223     195      33      27     479     168      57

Cuts at:   2549    2578    2616 
   Size:         29      38

  Fragments arranged by size: 

                 479     438     329     233     223     195     168     138
                  99      82      57      46      38      33      29      27
                   1       1
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0      84    1905    1929    2616 
   Size:         84    1821      24     687

  Fragments arranged by size: 

                1821     687      84      24
 
FspI TGC'GCA

Cuts at:      0    2221    2616 
   Size:       2221     395
 
FspAI rTGC'GCAy

Cuts at:      0    2221    2616 
   Size:       2221     395
 
HaeII r_GCGC'y

Cuts at:      0     118     191    1406    2002    2110    2476    2616 
   Size:        118      73    1215     596     108     366     140

  Fragments arranged by size: 

                1215     596     366     140     118     108      73
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1054    1086    1720    1752    2194    2226    2497    2509 
   Size:       1054      32     634      32     442      32     271      12

Cuts at:   2509    2529    2541    2616 
   Size:         20      12      75

  Fragments arranged by size: 

                1054     634     442     271      75      32      32      32
                  20      12      12
 
HincII GTy'rAC

Cuts at:      0    2040    2616 
   Size:       2040     576
 
KasI G'GCGC_C

Cuts at:      0     187    1998    2106    2472    2616 
   Size:        187    1811     108     366     144

  Fragments arranged by size: 

                1811     366     187     144     108
 
KpnI G_GTAC'C

Cuts at:      0     462    2616 
   Size:        462    2154
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    2552    2616 
   Size:       2552      64
 
MslI CAynn'nnrTG

Cuts at:      0     520     541     804    2616 
   Size:        520      21     263    1812

  Fragments arranged by size: 

                1812     520     263      21
 
MspA1I CmG'CkG

Cuts at:      0    1161    1170    1938    2408    2456    2616 
   Size:       1161       9     768     470      48     160

  Fragments arranged by size: 

                1161     768     470     160      48       9
 
NaeI GCC'GGC

Cuts at:      0     129     555    1719    1997    2616 
   Size:        129     426    1164     278     619

  Fragments arranged by size: 

                1164     619     426     278     129
 
NarI GG'CG_CC

Cuts at:      0     188    1999    2107    2473    2616 
   Size:        188    1811     108     366     143

  Fragments arranged by size: 

                1811     366     188     143     108
 
NcoI C'CATG_G

Cuts at:      0    1895    2616 
   Size:       1895     721
 
NgoMIV G'CCGG_C

Cuts at:      0     127     553    1717    1995    2616 
   Size:        127     426    1164     278     621

  Fragments arranged by size: 

                1164     621     426     278     127
 
NotI GC'GGCC_GC

Cuts at:      0     259    2616 
   Size:        259    2357
 
NspI r_CATG'y

Cuts at:      0    1734    1935    2038    2616 
   Size:       1734     201     103     578

  Fragments arranged by size: 

                1734     578     201     103
 
PasI CC'CwG_GG

Cuts at:      0     561    2616 
   Size:        561    2055
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1448    1776    2150    2616 
   Size:       1448     328     374     466

  Fragments arranged by size: 

                1448     466     374     328
 
PfoI T'CCnGG_A

Cuts at:      0     383    2616 
   Size:        383    2233
 
PmlI CAC'GTG

Cuts at:      0    1076    2616 
   Size:       1076    1540
 
PpuMI rG'GwC_Cy

Cuts at:      0    1221    1367    1590    1845    2578    2616 
   Size:       1221     146     223     255     733      38

  Fragments arranged by size: 

                1221     733     255     223     146      38
 
PspOMI G'GGCC_C

Cuts at:      0     371    1267    1785    1818    2548    2605    2616 
   Size:        371     896     518      33     730      57      11

  Fragments arranged by size: 

                 896     730     518     371      57      33      11
 
PspXI vC'TCGA_Gb

Cuts at:      0    1417    2616 
   Size:       1417    1199
 
PstI C_TGCA'G

Cuts at:      0     233     611     927    1757    2323    2616 
   Size:        233     378     316     830     566     293

  Fragments arranged by size: 

                 830     566     378     316     293     233
 
PvuII CAG'CTG

Cuts at:      0    1170    2456    2616 
   Size:       1170    1286     160

  Fragments arranged by size: 

                1286    1170     160
 
SacI G_AGCT'C

Cuts at:      0     364    2616 
   Size:        364    2252
 
SalI G'TCGA_C

Cuts at:      0    2038    2616 
   Size:       2038     578
 
SanDI GG'GwC_CC

Cuts at:      0    1367    2578    2616 
   Size:       1367    1211      38

  Fragments arranged by size: 

                1367    1211      38
 
SapI GCTCTTCn'nnn_

Cuts at:      0     352    2616 
   Size:        352    2264
 
SbfI CC_TGCA'GG

Cuts at:      0     927    2616 
   Size:        927    1689
 
ScaI AGT'ACT

Cuts at:      0     421    1084    1711    2616 
   Size:        421     663     627     905

  Fragments arranged by size: 

                 905     663     627     421
 
SfcI C'TryA_G

Cuts at:      0     229     607     923    1753    2319    2616 
   Size:        229     378     316     830     566     297

  Fragments arranged by size: 

                 830     566     378     316     297     229
 
SfoI GGC'GCC

Cuts at:      0     189    2000    2108    2474    2616 
   Size:        189    1811     108     366     142

  Fragments arranged by size: 

                1811     366     189     142     108
 
SgrAI Cr'CCGG_yG

Cuts at:      0    2569    2616 
   Size:       2569      47
 
SmaI CCC'GGG

Cuts at:      0     106    1601    2315    2616 
   Size:        106    1495     714     301

  Fragments arranged by size: 

                1495     714     301     106
 
SmlI C'TyrA_G

Cuts at:      0     481    1189    1417    2616 
   Size:        481     708     228    1199

  Fragments arranged by size: 

                1199     708     481     228
 
StuI AGG'CCT

Cuts at:      0    1013    1969    2616 
   Size:       1013     956     647

  Fragments arranged by size: 

                1013     956     647
 
StyI C'CwwG_G

Cuts at:      0    1355    1814    1895    2429    2616 
   Size:       1355     459      81     534     187

  Fragments arranged by size: 

                1355     534     459     187      81
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1413    1600    1978    2616 
   Size:       1413     187     378     638

  Fragments arranged by size: 

                1413     638     378     187
 
TatI w'GTAC_w

Cuts at:      0     419     749    1082    1112    1709    2616 
   Size:        419     330     333      30     597     907

  Fragments arranged by size: 

                 907     597     419     333     330      30
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    2173    2205    2616 
   Size:       2173      32     411

  Fragments arranged by size: 

                2173     411      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     543    2373    2436    2616 
   Size:        543    1830      63     180

  Fragments arranged by size: 

                1830     543     180      63
 
XhoI C'TCGA_G

Cuts at:      0    1417    2616 
   Size:       1417    1199
 
XmaI C'CCGG_G

Cuts at:      0     104    1599    2313    2616 
   Size:        104    1495     714     303

  Fragments arranged by size: 

                1495     714     303     104
 
XmnI GAAnn'nnTTC

Cuts at:      0     499    2098    2616 
   Size:        499    1599     518

  Fragments arranged by size: 

                1599     518     499
 
ZraI GAC'GTC

Cuts at:      0    1698    2616 
   Size:       1698     918
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AcuI   AflIII     AhdI     AleI     AlfI
    AlwNI     ApaI     ApoI     AscI     AvaI     BaeI     BanI    BanII
     BbeI     BbsI    BbvCI     BclI     BglI Bme1580I    BmgBI     BmrI
     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI    BsmBI
 Bsp1286I    BspEI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII
    BssSI   BstAPI   BstEII    BstXI    BstYI  BstZ17I     BtgI    BtgZI
     BtsI     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI
 EcoO109I     FalI     FspI    FspAI    HaeII    Hin4I   HincII     KasI
     KpnI     MmeI     MslI   MspA1I     NaeI     NarI     NcoI   NgoMIV
     NotI     NspI     PasI    PflMI     PfoI     PmlI    PpuMI   PspOMI
    PspXI     PstI    PvuII     SacI     SalI    SanDI     SapI     SbfI
     ScaI     SfcI     SfoI    SgrAI     SmaI     SmlI     StuI     StyI
    TaqII     TatI     TstI     XcmI     XhoI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AclI     AfeI    AflII     AgeI     AloI    ApaLI     AseI
    AsiSI    AvrII    BamHI     BcgI    BciVI    BfrBI    BglII     BlpI
     BmtI    BsiWI     BsmI    BspHI    BstBI   Bsu36I     ClaI    CspCI
     DraI   DraIII     DrdI    EcoRI    EcoRV     FseI  HindIII     HpaI
     MfeI     MluI     MscI     NdeI     NheI     NruI     NsiI     PacI
     PciI     PmeI     PpiI    PshAI     PsiI     PsrI     PvuI    RsrII
    SacII    SexAI     SfiI     SgfI    SnaBI     SpeI     SphI     SrfI
     SspI     SwaI  Tth111I     XbaI
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