Restriction map for pF1KA1082
 (Linear) (Six-base) MAPSORT of: KIAA1082.seq  Check: 9565  from: 1  to: 5283



KIAA1082.orf

 With 182 enzymes: SgfI * 

                        December 5, 2008 09:40  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1143    5193    5283 
   Size:       1143    4050      90

  Fragments arranged by size: 

                4050    1143      90
 
AccI GT'mk_AC

Cuts at:      0     926    1030    3384    4328    5283 
   Size:        926     104    2354     944     955

  Fragments arranged by size: 

                2354     955     944     926     104
 
Acc65I G'GTAC_C

Cuts at:      0    2762    4955    5283 
   Size:       2762    2193     328

  Fragments arranged by size: 

                2762    2193     328
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    2521    2692    3584    5283 
   Size:       2521     171     892    1699

  Fragments arranged by size: 

                2521    1699     892     171
 
AflIII A'CryG_T

Cuts at:      0    5124    5283 
   Size:       5124     159
 
AhdI GACnn_n'nnGTC

Cuts at:      0    3412    5283 
   Size:       3412    1871
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      52    4865    5283 
   Size:         52    4813     418

  Fragments arranged by size: 

                4813     418      52
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1315    1333    4349    5283 
   Size:       1315      18    3016     934

  Fragments arranged by size: 

                3016    1315     934      18
 
ApaI G_GGCC'C

Cuts at:      0     968    2143    2539    4612    5283 
   Size:        968    1175     396    2073     671

  Fragments arranged by size: 

                2073    1175     968     671     396
 
ApoI r'AATT_y

Cuts at:      0     202    1380    1389    2507    4309    5155    5283 
   Size:        202    1178       9    1118    1802     846     128

  Fragments arranged by size: 

                1802    1178    1118     846     202     128       9
 
AvaI C'yCGr_G

Cuts at:      0     131    1371    1886    2023    3341    5283 
   Size:        131    1240     515     137    1318    1942

  Fragments arranged by size: 

                1942    1318    1240     515     137     131
 
AvrII C'CTAG_G

Cuts at:      0    5028    5283 
   Size:       5028     255
 
BanI G'GyrC_C

Cuts at:      0     174     294    2010    2721    2762    3501    4955    5245 
   Size:        174     120    1716     711      41     739    1454     290

Cuts at:   5245    5283 
   Size:         38

  Fragments arranged by size: 

                1716    1454     739     711     290     174     120      41
                  38
 
BanII G_rGCy'C

Cuts at:      0     968    1696    2122    2143    2539    2690    4283    4612 
   Size:        968     728     426      21     396     151    1593     329

Cuts at:   4612    5061    5283 
   Size:        449     222

  Fragments arranged by size: 

                1593     968     728     449     426     396     329     222
                 151      21
 
BbeI G_GCGC'C

Cuts at:      0     298    5283 
   Size:        298    4985
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    3945    5111    5283 
   Size:       3945    1166     172

  Fragments arranged by size: 

                3945    1166     172
 
BbvCI CC'TCA_GC

Cuts at:      0      66     251    3966    5283 
   Size:         66     185    3715    1317

  Fragments arranged by size: 

                3715    1317     185      66
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    2722    2756    4762    4796    5283 
   Size:       2722      34    2006      34     487

  Fragments arranged by size: 

                2722    2006     487      34      34
 
BclI T'GATC_A

Cuts at:      0     542    4926    5283 
   Size:        542    4384     357

  Fragments arranged by size: 

                4384     542     357
 
BfrBI ATG'CAT

Cuts at:      0    1586    2711    5283 
   Size:       1586    1125    2572

  Fragments arranged by size: 

                2572    1586    1125
 
BglI GCCn_nnn'nGGC

Cuts at:      0     142    5283 
   Size:        142    5141
 
BglII A'GATC_T

Cuts at:      0    2946    3559    4208    4509    5283 
   Size:       2946     613     649     301     774

  Fragments arranged by size: 

                2946     774     649     613     301
 
BlpI GC'TnA_GC

Cuts at:      0    2121    2558    5283 
   Size:       2121     437    2725

  Fragments arranged by size: 

                2725    2121     437
 
Bme1580I G_kGCm'C

Cuts at:      0     179     968    2143    2539    4612    5283 
   Size:        179     789    1175     396    2073     671

  Fragments arranged by size: 

                2073    1175     789     671     396     179
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     304     394    3135    5283 
   Size:        304      90    2741    2148

  Fragments arranged by size: 

                2741    2148     304      90
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1441    2049    5283 
   Size:       1441     608    3234

  Fragments arranged by size: 

                3234    1441     608
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     304    1790    2157    2418    2702    2776    3500    5075 
   Size:        304    1486     367     261     284      74     724    1575

Cuts at:   5075    5102    5283 
   Size:         27     181

  Fragments arranged by size: 

                1575    1486     724     367     304     284     261     181
                  74      27
 
Bpu10I CC'TnA_GC

Cuts at:      0      66     251    1690    1695    3175    3966    5283 
   Size:         66     185    1439       5    1480     791    1317

  Fragments arranged by size: 

                1480    1439    1317     791     185      66       5
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1702    4255    4438    4744    5236    5283 
   Size:       1702    2553     183     306     492      47

  Fragments arranged by size: 

                2553    1702     492     306     183      47
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     287    2947    3399    3801    3875    4310    5283 
   Size:        287    2660     452     402      74     435     973

  Fragments arranged by size: 

                2660     973     452     435     402     287      74
 
BsaBI GATnn'nnATC

Cuts at:      0    4726    5283 
   Size:       4726     557
 
BsaHI Gr'CG_yC

Cuts at:      0     295    5283 
   Size:        295    4988
 
BsaWI w'CCGG_w

Cuts at:      0    1769    2765    2825    5283 
   Size:       1769     996      60    2458

  Fragments arranged by size: 

                2458    1769     996      60
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     351     381     849     879    1788    1808    1818    1838 
   Size:        351      30     468      30     909      20      10      20

Cuts at:   1838    2416    2446    3314    3344    5283 
   Size:        578      30     868      30    1939

  Fragments arranged by size: 

                1939     909     868     578     468     351      30      30
                  30      30      20      20      10
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     870    1968    2025    2082    2214    2254    2272    2488 
   Size:        870    1098      57      57     132      40      18     216

Cuts at:   2488    3506    3645    5005    5283 
   Size:       1018     139    1360     278

  Fragments arranged by size: 

                1360    1098    1018     870     278     216     139     132
                  57      57      40      18
 
BseYI C'CCAG_C

Cuts at:      0    2014    2532    2568    2882    3834    4186    5009    5283 
   Size:       2014     518      36     314     952     352     823     274

  Fragments arranged by size: 

                2014     952     823     518     352     314     274      36
            
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     500    1126    1517    2716    3649    5041    5283 
   Size:        500     626     391    1199     933    1392     242

  Fragments arranged by size: 

                1392    1199     933     626     500     391     242
 
BsiEI CG_ry'CG

Cuts at:      0    1363    5283 
   Size:       1363    3920
 
BsiHKAI G_wGCw'C

Cuts at:      0    1422    1696    2519    3740    4283    4448    4743    5283 
   Size:       1422     274     823    1221     543     165     295     540

  Fragments arranged by size: 

                1422    1221     823     543     540     295     274     165
            
 
BsmI GAATG_Cn'

Cuts at:      0     326     987    2752    5283 
   Size:        326     661    1765    2531

  Fragments arranged by size: 

                2531    1765     661     326
 
Bsp1286I G_dGCh'C

Cuts at:      0     179     968    1422    1696    2122    2143    2519    2539 
   Size:        179     789     454     274     426      21     376      20

Cuts at:   2539    2690    3740    4283    4448    4612    4743    5061    5283 
   Size:        151    1050     543     165     164     131     318     222

  Fragments arranged by size: 

                1050     789     543     454     426     376     318     274
                 222     179     165     164     151     131      21      20
            
 
BspEI T'CCGG_A

Cuts at:      0    2825    5283 
   Size:       2825    2458
 
BspHI T'CATG_A

Cuts at:      0    1851    4743    4809    4929    4941    5187    5259    5283 
   Size:       1851    2892      66     120      12     246      72      24

  Fragments arranged by size: 

                2892    1851     246     120      72      66      24      12
            
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1143    3417    5058    5193    5283 
   Size:       1143    2274    1641     135      90

  Fragments arranged by size: 

                2274    1641    1143     135      90
 
BsrBI CCG'CTC

Cuts at:      0     104     167    2171    2760    5283 
   Size:        104      63    2004     589    2523

  Fragments arranged by size: 

                2523    2004     589     104      63
 
BsrDI GCAATG_nn'

Cuts at:      0     338    4016    5283 
   Size:        338    3678    1267

  Fragments arranged by size: 

                3678    1267     338
 
BsrFI r'CCGG_y

Cuts at:      0     145    1358    2603    3170    3185    3498    5283 
   Size:        145    1213    1245     567      15     313    1785

  Fragments arranged by size: 

                1785    1245    1213     567     313     145      15
 
BsrGI T'GTAC_A

Cuts at:      0    4619    5283 
   Size:       4619     664
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1477    3030    4312    5283 
   Size:       1477    1553    1282     971

  Fragments arranged by size: 

                1553    1477    1282     971
 
BstYI r'GATC_y

Cuts at:      0     108     726    1304    1714    2946    3559    4128    4208 
   Size:        108     618     578     410    1232     613     569      80

Cuts at:   4208    4509    4871    5283 
   Size:        301     362     412

  Fragments arranged by size: 

                1232     618     613     578     569     412     410     362
                 301     108      80
 
Bsu36I CC'TnA_GG

Cuts at:      0    3727    5283 
   Size:       3727    1556
 
BtgI C'CryG_G

Cuts at:      0      86    1816    2156    5283 
   Size:         86    1730     340    3127

  Fragments arranged by size: 

                3127    1730     340      86
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     956    1421    5283 
   Size:        956     465    3862

  Fragments arranged by size: 

                3862     956     465
 
BtsI GCAGTG_nn'

Cuts at:      0      56    2559    3201    3785    3842    5234    5283 
   Size:         56    2503     642     584      57    1392      49

  Fragments arranged by size: 

                2503    1392     642     584      57      56      49
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    3112    3147    5283 
   Size:       3112      35    2136

  Fragments arranged by size: 

                3112    2136      35
 
DraIII CAC_nnn'GTG

Cuts at:      0    3196    5283 
   Size:       3196    2087
 
EaeI y'GGCC_r

Cuts at:      0     349     849    1239    2166    2631    2972    3187    5283 
   Size:        349     500     390     927     465     341     215    2096

  Fragments arranged by size: 

                2096     927     500     465     390     349     341     215
            
 
EarI CTCTTCn'nnn_

Cuts at:      0     817    1410    1886    1986    2080    2928    3118    3200 
   Size:        817     593     476     100      94     848     190      82

Cuts at:   3200    3465    3560    3973    4650    4745    5283 
   Size:        265      95     413     677      95     538

  Fragments arranged by size: 

                 848     817     677     593     538     476     413     265
                 190     100      95      95      94      82
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      19    3503    3648    5131    5283 
   Size:         19    3484     145    1483     152

  Fragments arranged by size: 

                3484    1483     152     145      19
 
EcoICRI GAG'CTC

Cuts at:      0    1694    4281    5283 
   Size:       1694    2587    1002

  Fragments arranged by size: 

                2587    1694    1002
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     304    1790    2157    2418    2521    2692    2702    2776 
   Size:        304    1486     367     261     103     171      10      74

Cuts at:   2776    3500    3584    5075    5102    5283 
   Size:        724      84    1491      27     181

  Fragments arranged by size: 

                1491    1486     724     367     304     261     181     171
                 103      84      74      27      10
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     319    5149    5283 
   Size:        319    4830     134

  Fragments arranged by size: 

                4830     319     134
 
EcoO109I rG'GnC_Cy

Cuts at:      0     115     183     306     580     964     965    2139    2257 
   Size:        115      68     123     274     384       1    1174     118

Cuts at:   2257    2536    2592    3722    3838    4609    5283 
   Size:        279      56    1130     116     771     674

  Fragments arranged by size: 

                1174    1130     771     674     384     279     274     123
                 118     116     115      68      56       1
 
EcoRI G'AATT_C

Cuts at:      0    1380    2507    5155    5283 
   Size:       1380    1127    2648     128

  Fragments arranged by size: 

                2648    1380    1127     128
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     301    2429    5283 
   Size:        301    2128    2854

  Fragments arranged by size: 

                2854    2128     301
 
FseI GG_CCGG'CC

Cuts at:      0    2607    5283 
   Size:       2607    2676
 
FspI TGC'GCA

Cuts at:      0     127    5283 
   Size:        127    5156
 
HaeII r_GCGC'y

Cuts at:      0     298    5283 
   Size:        298    4985
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      93     125     351     383     877     909    2035    2067 
   Size:         93      32     226      32     494      32    1126      32

Cuts at:   2067    2202    2234    2416    2448    2826    2858    3137    3169 
   Size:        135      32     182      32     378      32     279      32

Cuts at:   3169    3398    3430    3544    3576    5098    5130    5283 
   Size:        229      32     114      32    1522      32     153

  Fragments arranged by size: 

                1522    1126     494     378     279     229     226     182
                 153     135     114      93      32      32      32      32
                  32      32      32      32      32      32      32
 
HincII GTy'rAC

Cuts at:      0    3385    5283 
   Size:       3385    1898
 
HindIII A'AGCT_T

Cuts at:      0    2406    3814    5283 
   Size:       2406    1408    1469

  Fragments arranged by size: 

                2406    1469    1408
 
KasI G'GCGC_C

Cuts at:      0     294    5283 
   Size:        294    4989
 
KpnI G_GTAC'C

Cuts at:      0    2766    4959    5283 
   Size:       2766    2193     324

  Fragments arranged by size: 

                2766    2193     324
 
MfeI C'AATT_G

Cuts at:      0    4200    4766    5283 
   Size:       4200     566     517

  Fragments arranged by size: 

                4200     566     517
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    3875    4204    4875    5283 
   Size:       3875     329     671     408

  Fragments arranged by size: 

                3875     671     408     329
 
MscI TGG'CCA

Cuts at:      0     351    1241    2633    2974    5283 
   Size:        351     890    1392     341    2309

  Fragments arranged by size: 

                2309    1392     890     351     341
 
MslI CAynn'nnrTG

Cuts at:      0    2209    2716    3028    4682    4847    5283 
   Size:       2209     507     312    1654     165     436

  Fragments arranged by size: 

                2209    1654     507     436     312     165
 
MspA1I CmG'CkG

Cuts at:      0      88      97    1264    2018    2481    3804    5283 
   Size:         88       9    1167     754     463    1323    1479

  Fragments arranged by size: 

                1479    1323    1167     754     463      88       9
 
NaeI GCC'GGC

Cuts at:      0     147    2605    3187    5283 
   Size:        147    2458     582    2096

  Fragments arranged by size: 

                2458    2096     582     147
 
NarI GG'CG_CC

Cuts at:      0     295    5283 
   Size:        295    4988
 
NcoI C'CATG_G

Cuts at:      0    2156    5283 
   Size:       2156    3127
 
NdeI CA'TA_TG

Cuts at:      0    4405    5283 
   Size:       4405     878
 
NgoMIV G'CCGG_C

Cuts at:      0     145    2603    3185    5283 
   Size:        145    2458     582    2098

  Fragments arranged by size: 

                2458    2098     582     145
 
NsiI A_TGCA'T

Cuts at:      0    1588    2713    5283 
   Size:       1588    1125    2570

  Fragments arranged by size: 

                2570    1588    1125
 
NspI r_CATG'y

Cuts at:      0     514    1590    3337    5128    5283 
   Size:        514    1076    1747    1791     155

  Fragments arranged by size: 

                1791    1747    1076     514     155
 
PciI A'CATG_T

Cuts at:      0    5124    5283 
   Size:       5124     159
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    3051    5283 
   Size:       3051    2232
 
PfoI T'CCnGG_A

Cuts at:      0    4214    4874    5283 
   Size:       4214     660     409

  Fragments arranged by size: 

                4214     660     409
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     421     453    5283 
   Size:        421      32    4830

  Fragments arranged by size: 

                4830     421      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     183     306     580    3722    3838    5283 
   Size:        183     123     274    3142     116    1445

  Fragments arranged by size: 

                3142    1445     274     183     123     116
 
PshAI GACnn'nnGTC

Cuts at:      0    4419    5283 
   Size:       4419     864
 
PspOMI G'GGCC_C

Cuts at:      0     964    2139    2535    4608    5283 
   Size:        964    1175     396    2073     675

  Fragments arranged by size: 

                2073    1175     964     675     396
 
PspXI vC'TCGA_Gb

Cuts at:      0     131    5283 
   Size:        131    5152
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0    2096    2128    5203    5235    5283 
   Size:       2096      32    3075      32      48

  Fragments arranged by size: 

                3075    2096      48      32      32
 
PstI C_TGCA'G

Cuts at:      0    1785    2043    2486    2699    4345    5204    5283 
   Size:       1785     258     443     213    1646     859      79

  Fragments arranged by size: 

                1785    1646     859     443     258     213      79
 
PvuII CAG'CTG

Cuts at:      0    1264    2018    2481    5283 
   Size:       1264     754     463    2802

  Fragments arranged by size: 

                2802    1264     754     463
 
SacI G_AGCT'C

Cuts at:      0    1696    4283    5283 
   Size:       1696    2587    1000

  Fragments arranged by size: 

                2587    1696    1000
 
SacII CC_GC'GG

Cuts at:      0      89    5283 
   Size:         89    5194
 
SalI G'TCGA_C

Cuts at:      0    3383    5283 
   Size:       3383    1900
 
SanDI GG'GwC_CC

Cuts at:      0    3722    3838    5283 
   Size:       3722     116    1445

  Fragments arranged by size: 

                3722    1445     116
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1886    3560    3973    5283 
   Size:       1886    1674     413    1310

  Fragments arranged by size: 

                1886    1674    1310     413
 
SbfI CC_TGCA'GG

Cuts at:      0    2699    5283 
   Size:       2699    2584
 
SexAI A'CCwGG_T

Cuts at:      0    4498    5066    5283 
   Size:       4498     568     217

  Fragments arranged by size: 

                4498     568     217
 
SfcI C'TryA_G

Cuts at:      0     585    1118    1157    1524    1781    2039    2482    2695 
   Size:        585     533      39     367     257     258     443     213

Cuts at:   2695    2801    4341    5200    5283 
   Size:        106    1540     859      83

  Fragments arranged by size: 

                1540     859     585     533     443     367     258     257
                 213     106      83      39
 
SfoI GGC'GCC

Cuts at:      0     296    5283 
   Size:        296    4987
 
SmaI CCC'GGG

Cuts at:      0    3343    5283 
   Size:       3343    1940
 
SmlI C'TyrA_G

Cuts at:      0     131    1681    4270    4453    4759    5251    5283 
   Size:        131    1550    2589     183     306     492      32

  Fragments arranged by size: 

                2589    1550     492     306     183     131      32
 
SspI AAT'ATT

Cuts at:      0    3318    5283 
   Size:       3318    1965
 
StuI AGG'CCT

Cuts at:      0    1369    1700    2498    3627    4599    5283 
   Size:       1369     331     798    1129     972     684

  Fragments arranged by size: 

                1369    1129     972     798     684     331
 
StyI C'CwwG_G

Cuts at:      0    2003    2156    2597    3923    4856    5028    5283 
   Size:       2003     153     441    1326     933     172     255

  Fragments arranged by size: 

                2003    1326     933     441     255     172     153
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     869    1183    2028    3202    5283 
   Size:        869     314     845    1174    2081

  Fragments arranged by size: 

                2081    1174     869     845     314
 
TatI w'GTAC_w

Cuts at:      0    4619    5283 
   Size:       4619     664
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     622     654     849     865     881     897     972    1004 
   Size:        622      32     195      16      16      16      75      32

Cuts at:   1004    1788    1808    1820    1840    5283 
   Size:        784      20      12      20    3443

  Fragments arranged by size: 

                3443     784     622     195      75      32      32      20
                  20      16      16      16      12
 
Tth111I GACn'n_nGTC

Cuts at:      0     310    5283 
   Size:        310    4973
 
XbaI T'CTAG_A

Cuts at:      0    2949    5283 
   Size:       2949    2334
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    3030    4835    5283 
   Size:       3030    1805     448

  Fragments arranged by size: 

                3030    1805     448
 
XhoI C'TCGA_G

Cuts at:      0     131    5283 
   Size:        131    5152
 
XmaI C'CCGG_G

Cuts at:      0    3341    5283 
   Size:       3341    1942
 
XmnI GAAnn'nnTTC

Cuts at:      0     416    2386    4210    4478    5283 
   Size:        416    1970    1824     268     805

  Fragments arranged by size: 

                1970    1824     805     416     268
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AcuI   AflIII     AhdI     AlfI    AlwNI
     ApaI     ApoI     AvaI    AvrII     BanI    BanII     BbeI     BbsI
    BbvCI     BcgI     BclI    BfrBI     BglI    BglII     BlpI Bme1580I
     BmrI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaBI    BsaHI
    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI
 Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI
    BstXI    BstYI   Bsu36I     BtgI    BtgZI     BtsI    CspCI   DraIII
     EaeI     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI
     FalI     FseI     FspI    HaeII    Hin4I   HincII  HindIII     KasI
     KpnI     MfeI     MmeI     MscI     MslI   MspA1I     NaeI     NarI
     NcoI     NdeI   NgoMIV     NsiI     NspI     PciI    PflMI     PfoI
     PpiI    PpuMI    PshAI   PspOMI    PspXI     PsrI     PstI    PvuII
     SacI    SacII     SalI    SanDI     SapI     SbfI    SexAI     SfcI
     SfoI     SmaI     SmlI     SspI     StuI     StyI    TaqII     TatI
     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI

 Enzymes that do not cut: 

    AatII     AclI     AfeI    AflII     AgeI     AleI     AloI    ApaLI
     AscI     AseI    AsiSI     BaeI    BamHI    BciVI    BmgBI     BmtI
    BsaAI    BsiWI    BsmBI   BssHII    BssSI   BstAPI    BstBI   BstEII
  BstZ17I     ClaI     DraI     DrdI     EagI    EcoRV    FspAI     HpaI
     MluI     NheI     NotI     NruI     PacI     PasI     PmeI     PmlI
     PsiI     PvuI    RsrII     ScaI     SfiI     SgfI    SgrAI    SnaBI
     SpeI     SphI     SrfI     SwaI     ZraI
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