Restriction map for pF1KB7332
##################################
# NO CUT ENZYMES (  109 enzymes) #
##################################

AatII       AccIII      AcyI        AflII       AgeI        
AhdI        Alw44I      AlwNI       AocI        Aor51HI     
ApaLI       AscI        AseI        AsnI        AspEI       
AspI        AvrII       BalI        BbeI        BbrPI       
BbvCI       BcgI        BciVI       BfrI        BglI        
BlnI        BlpI        Bpu1102I    BsaAI       BsaBI       
BsaHI       BsaI        BseAI       BsiWI       BspDI       
BspEI       BspHI       BspMI       Bst1107I    BstBI       
BstEII      BstPI       BstXI       BstZ17I     Bsu36I      
CelII       ClaI        CpoI        DraIII      DrdI        
Eam1105I    Eco47III    Eco81I      EcoNI       EcoO65I     
EcoRV       HaeII       Hin1I       HpaI        KasI        
KspI        MamI        MfeI        MluI        MluNI       
MroI        MscI        MunI        NarI        NcoI        
NheI        NotI        NruI        NspV        PacI        
PaeR7I      PflFI       PflMI       PinAI       PmaCI       
PmeI        PmlI        PshAI       PshBI       PvuI        
RcaI        RsrII       SacII       SalI        SapI        
SbfI        ScaI        SexAI       SfiI        SfoI        
SfuI        SgrAI       SnaBI       SpeI        Sse8387I    
SspI        StuI        SwaI        TaqI        Tth111I     
TthHB8I     Van91I      XbaI        XhoI        


###################################
# ONE CUT ENZYMES (   50 enzymes) #
###################################

Acc65I       2481  GGTACC               G^GTACC
AccI          679  GTCTAC               GT^[AC][GT]AC
AclI         2918  AACGTT               AA^CGTT
AflIII       2025  ACATGT               A^C[GA][CT]GT
Asp700I      1255  GAAGGACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I      2481  GGTACC               G^GTACC
AviII        2877  TGCGCA               TGC^GCA
BamHI         415  GGATCC               G^GATCC
BanI         2481  GGTACC               G^G[CT][GA]CC
BbsI         1962  GAAGAC               GAAGAC(2/6)
BclI          245  TGATCA               T^GATCA
BfaI          202  CTAG                 C^TAG
BpuAI        1962  GAAGAC               GAAGAC(2/6)
BsaWI        2630  TCCGGT               [AT]^CCGG[AT]
BsmBI          52  GAGACG               CGTCTC(1/5)
Bsp1407I     1022  TGTACA               T^GTACA
BspLU11I     2025  ACATGT               A^CATGT
BsrBI        2210  GAGCGG               CCGCTC(-3/-3)
BsrGI        1022  TGTACA               T^GTACA
BssHII        119  GCGCGC               G^CGCGC
BssSI         970  CTCGTG               CACGAG(-5/-1)
BstAPI       1345  GCAGTTGGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
EagI           90  CGGCCG               C^GGCCG
EclXI          90  CGGCCG               C^GGCCG
Eco52I         90  CGGCCG               C^GGCCG
EcoRI        1783  GAATTC               G^AATTC
EcoT14I      1746  CCTTGG               C^C[AT][AT]GG
EcoT22I      2006  ATGCAT               ATGCA^T
FauI         1704  GCGGG                CCCGC(4/6)
FbaI          245  TGATCA               T^GATCA
FseI         2761  GGCCGGCC             GGCCGG^CC
FspI         2877  TGCGCA               TGC^GCA
HgaI         1408  GACGC                GACGC(5/10)
HindIII       901  AAGCTT               A^AGCTT
KpnI         2481  GGTACC               GGTAC^C
MaeI          202  CTAG                 C^TAG
MspA1I        313  CAGCTG               C[AC]G^C[GT]G
NaeI         2762  GCCGGC               GCC^GGC
NgoMIV       2762  GCCGGC               G^CCGGC
NsiI         2006  ATGCAT               ATGCA^T
Psp1406I     2918  AACGTT               AA^CGTT
PstI         2130  CTGCAG               CTGCA^G
PvuII         313  CAGCTG               CAG^CTG
SmaI          253  CCCGGG               CCC^GGG
SphI         2238  GCATGC               GCATG^C
SspBI        1022  TGTACA               T^GTACA
StyI         1746  CCTTGG               C^C[AT][AT]GG
XmaCI         253  CCCGGG               C^CCGGG
XmaI          253  CCCGGG               C^CCGGG
XmnI         1255  GAAGGACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   20 enzymes) #
###################################

AccII          19  CGCG                 CG^CG
              120  CGCG                
ApaI          135  GGGCCC               GGGCC^C
              641  GGGCCC              
AvaI          253  CCCGGG               C^[CT]CG[GA]G
             1038  CTCGGG              
BglII         963  AGATCT               A^GATCT
             1488  AGATCT              
BseRI         815  CTCCTC               GAGGAG(10/8)
              809  GAGGAG              
BsgI          172  CTGCAC               GTGCAG(16/14)
             1217  GTGCAG              
BsmFI        1706  GGGAC                GGGAC(10/14)
               82  GTCCC               
BsmI         1053  GAATGC               GAATGC(1/-1)
             2551  GAATGC              
BsoBI         253  CCCGGG               C^[CT]CG[GA]G
             1038  CTCGGG              
BsrFI          88  ACCGGC               [GA]^CCGG[CT]
             2762  GCCGGC              
BstUI          19  CGCG                 CG^CG
              120  CGCG                
Cfr10I         88  ACCGGC               [GA]^CCGG[CT]
             2762  GCCGGC              
DsaI          180  CCACGG               C^C[GA][CT]GG
             2039  CCGTGG              
HincII       1233  GTCAAC               GT[CT]^[GA]AC
             1405  GTTGAC              
HindII       1233  GTCAAC               GT[CT]^[GA]AC
             1405  GTTGAC              
MslI         1641  CATACAGGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             2928  CACCATAGTG          
MvnI           19  CGCG                 CG^CG
              120  CGCG                
NdeI         2009  CATATG               CA^TATG
             2987  CATATG              
PpuMI          80  GGGTCCC              [GA]G^G[AT]CC[CT]
             2219  AGGACCT             
SmlI         2163  CTCAAG               C^T[CT][GA]AG
             2800  CTCAAG              


#########################################
# 3 OR MORE CUT ENZYMES (   79 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
ApoI        AspHI       AvaII       BanII       BbvI        
BcnI        BmyI        BpmI        BsaJI       BsiEI       
BsiHKAI     BsiYI       BslI        BsmAI       Bsp1286I    
BsrDI       BsrI        BssKI       BstF5I      BstNI       
BstYI       Cac8I       CfoI        Cfr13I      DdeI        
DpnII       DraI        DraII       EaeI        EarI        
EcoO109I    EcoRII      Fnu4HI      FokI        HaeIII      
HapII       HhaI        HinP1I      HinfI       HpaII       
HphI        ItaI        Ksp632I     MaeII       MaeIII      
MboI        MboII       MflI        MnlI        MseI        
MspI        MvaI        MwoI        NciI        NdeII       
NlaIII      NlaIV       NspI        PleI        RsaI        
SacI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        TfiI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       XcmI        XhoII       
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