Restriction map for pF1KB8996
 (Linear) (Six-base) MAPSORT of: KIBB8996.seq  Check: 3740  from: 1  to: 1221



KIBB8996.orf

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:50  ..

AleI CACnn'nnGTG

Cuts at:      0    1057    1221 
   Size:       1057     164
 
AlwNI CAG_nnn'CTG

Cuts at:      0     510     674     771    1221 
   Size:        510     164      97     450

  Fragments arranged by size: 

                 510     450     164      97
 
AvaI C'yCGr_G

Cuts at:      0     141     393     464    1221 
   Size:        141     252      71     757

  Fragments arranged by size: 

                 757     252     141      71
 
BamHI G'GATC_C

Cuts at:      0     136    1221 
   Size:        136    1085
 
BanI G'GyrC_C

Cuts at:      0     397     780     857    1221 
   Size:        397     383      77     364

  Fragments arranged by size: 

                 397     383     364      77
 
BanII G_rGCy'C

Cuts at:      0      85     200    1221 
   Size:         85     115    1021

  Fragments arranged by size: 

                1021     115      85
 
BbeI G_GCGC'C

Cuts at:      0     401    1221 
   Size:        401     820
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     847     881    1221 
   Size:        847      34     340

  Fragments arranged by size: 

                 847     340      34
 
BglI GCCn_nnn'nGGC

Cuts at:      0     706     944    1018    1221 
   Size:        706     238      74     203

  Fragments arranged by size: 

                 706     238     203      74
 
BlpI GC'TnA_GC

Cuts at:      0    1129    1221 
   Size:       1129      92
 
Bme1580I G_kGCm'C

Cuts at:      0    1190    1221 
   Size:       1190      31
 
BmgBI CAC'GTC

Cuts at:      0     747    1185    1221 
   Size:        747     438      36

  Fragments arranged by size: 

                 747     438      36
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     244     586     901    1221 
   Size:        244     342     315     320

  Fragments arranged by size: 

                 342     320     315     244
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     267     646    1221 
   Size:        267     379     575

  Fragments arranged by size: 

                 575     379     267
 
BsaHI Gr'CG_yC

Cuts at:      0     103     398    1221 
   Size:        103     295     823

  Fragments arranged by size: 

                 823     295     103
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     208     450    1032    1219    1221 
   Size:        208     242     582     187       2

  Fragments arranged by size: 

                 582     242     208     187       2
 
BseYI C'CCAG_C

Cuts at:      0      24      63     843    1008    1221 
   Size:         24      39     780     165     213

  Fragments arranged by size: 

                 780     213     165      39      24
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     313     769     853    1221 
   Size:        313     456      84     368

  Fragments arranged by size: 

                 456     368     313      84
 
BsiEI CG_ry'CG

Cuts at:      0      18      56     204     357    1221 
   Size:         18      38     148     153     864

  Fragments arranged by size: 

                 864     153     148      38      18
 
BsiHKAI G_wGCw'C

Cuts at:      0     200    1135    1221 
   Size:        200     935      86

  Fragments arranged by size: 

                 935     200      86
 
BsmI GAATG_Cn'

Cuts at:      0     863    1115    1221 
   Size:        863     252     106

  Fragments arranged by size: 

                 863     252     106
 
Bsp1286I G_dGCh'C

Cuts at:      0      85     200    1135    1190    1221 
   Size:         85     115     935      55      31

  Fragments arranged by size: 

                 935     115      85      55      31
 
BsrBI CCG'CTC

Cuts at:      0     720     729    1221 
   Size:        720       9     492

  Fragments arranged by size: 

                 720     492       9
 
BsrFI r'CCGG_y

Cuts at:      0      55     269     578     783     815     973    1221 
   Size:         55     214     309     205      32     158     248

  Fragments arranged by size: 

                 309     248     214     205     158      55      32
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     291    1221 
   Size:        291     930
 
BstYI r'GATC_y

Cuts at:      0     136     365    1221 
   Size:        136     229     856

  Fragments arranged by size: 

                 856     229     136
 
Bsu36I CC'TnA_GG

Cuts at:      0      71     296    1221 
   Size:         71     225     925

  Fragments arranged by size: 

                 925     225      71
 
BtgI C'CryG_G

Cuts at:      0     305     621     776    1221 
   Size:        305     316     155     445

  Fragments arranged by size: 

                 445     316     305     155
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     902    1221 
   Size:        902     319
 
BtsI GCAGTG_nn'

Cuts at:      0     757    1221 
   Size:        757     464
 
DraIII CAC_nnn'GTG

Cuts at:      0     291    1221 
   Size:        291     930
 
EaeI y'GGCC_r

Cuts at:      0      53     187     201     354     381     405     773     891 
   Size:         53     134      14     153      27      24     368     118

Cuts at:    891    1221 
   Size:        330

  Fragments arranged by size: 

                 368     330     153     134     118      53      27      24
                  14
 
EagI C'GGCC_G

Cuts at:      0      53     201     354    1221 
   Size:         53     148     153     867

  Fragments arranged by size: 

                 867     153     148      53
 
EarI CTCTTCn'nnn_

Cuts at:      0     643     800    1221 
   Size:        643     157     421

  Fragments arranged by size: 

                 643     421     157
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     460     597     925    1221 
   Size:        460     137     328     296

  Fragments arranged by size: 

                 460     328     296     137
 
EcoICRI GAG'CTC

Cuts at:      0     198    1221 
   Size:        198    1023
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     244     586     901    1221 
   Size:        244     342     315     320

  Fragments arranged by size: 

                 342     320     315     244
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     568    1221 
   Size:        568     653
 
EcoO109I rG'GnC_Cy

Cuts at:      0      67    1026    1221 
   Size:         67     959     195

  Fragments arranged by size: 

                 959     195      67
 
FseI GG_CCGG'CC

Cuts at:      0     273    1221 
   Size:        273     948
 
FspI TGC'GCA

Cuts at:      0     322    1221 
   Size:        322     899
 
HaeII r_GCGC'y

Cuts at:      0      25     401    1221 
   Size:         25     376     820

  Fragments arranged by size: 

                 820     376      25
 
KasI G'GCGC_C

Cuts at:      0     397    1221 
   Size:        397     824
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     515    1221 
   Size:        515     706
 
MslI CAynn'nnrTG

Cuts at:      0    1057    1141    1198    1221 
   Size:       1057      84      57      23

  Fragments arranged by size: 

                1057      84      57      23
 
MspA1I CmG'CkG

Cuts at:      0     671     761    1004    1221 
   Size:        671      90     243     217

  Fragments arranged by size: 

                 671     243     217      90
 
NaeI GCC'GGC

Cuts at:      0      57     271     580    1221 
   Size:         57     214     309     641

  Fragments arranged by size: 

                 641     309     214      57
 
NarI GG'CG_CC

Cuts at:      0     398    1221 
   Size:        398     823
 
NcoI C'CATG_G

Cuts at:      0     305    1221 
   Size:        305     916
 
NgoMIV G'CCGG_C

Cuts at:      0      55     269     578    1221 
   Size:         55     214     309     643

  Fragments arranged by size: 

                 643     309     214      55
 
NspI r_CATG'y

Cuts at:      0    1080    1221 
   Size:       1080     141
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     533     771    1221 
   Size:        533     238     450

  Fragments arranged by size: 

                 533     450     238
 
PfoI T'CCnGG_A

Cuts at:      0     221     563     608    1221 
   Size:        221     342      45     613

  Fragments arranged by size: 

                 613     342     221      45
 
PpuMI rG'GwC_Cy

Cuts at:      0    1026    1221 
   Size:       1026     195
 
PstI C_TGCA'G

Cuts at:      0     260    1221 
   Size:        260     961
 
PvuII CAG'CTG

Cuts at:      0     761    1004    1221 
   Size:        761     243     217

  Fragments arranged by size: 

                 761     243     217
 
SacI G_AGCT'C

Cuts at:      0     200    1221 
   Size:        200    1021
 
SanDI GG'GwC_CC

Cuts at:      0    1026    1221 
   Size:       1026     195
 
SfcI C'TryA_G

Cuts at:      0     256    1221 
   Size:        256     965
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0     706    1221 
   Size:        706     515
 
SfoI GGC'GCC

Cuts at:      0     399    1221 
   Size:        399     822
 
SmaI CCC'GGG

Cuts at:      0     143     395     466    1221 
   Size:        143     252      71     755

  Fragments arranged by size: 

                 755     252     143      71
 
SmlI C'TyrA_G

Cuts at:      0     246     661    1221 
   Size:        246     415     560

  Fragments arranged by size: 

                 560     415     246
 
SphI G_CATG'C

Cuts at:      0    1080    1221 
   Size:       1080     141
 
SrfI GCCC'GGGC

Cuts at:      0     395    1221 
   Size:        395     826
 
StuI AGG'CCT

Cuts at:      0      76     709     878    1221 
   Size:         76     633     169     343

  Fragments arranged by size: 

                 633     343     169      76
 
StyI C'CwwG_G

Cuts at:      0     305    1221 
   Size:        305     916
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     102     984    1221 
   Size:        102     882     237

  Fragments arranged by size: 

                 882     237     102
 
TatI w'GTAC_w

Cuts at:      0     602    1148    1163    1221 
   Size:        602     546      15      58

  Fragments arranged by size: 

                 602     546      58      15
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     978    1010    1221 
   Size:        978      32     211

  Fragments arranged by size: 

                 978     211      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1197    1221 
   Size:       1197      24
 
XmaI C'CCGG_G

Cuts at:      0     141     393     464    1221 
   Size:        141     252      71     757

  Fragments arranged by size: 

                 757     252     141      71
 

 Enzymes that do cut:

     AleI    AlwNI     AvaI    BamHI     BanI    BanII     BbeI     BcgI
     BglI     BlpI Bme1580I    BmgBI     BpmI    BpuEI    BsaHI    BseRI
    BseYI     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I    BsrBI    BsrFI
   BstAPI    BstYI   Bsu36I     BtgI    BtgZI     BtsI   DraIII     EaeI
     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I     FseI
     FspI    HaeII     KasI     MmeI     MslI   MspA1I     NaeI     NarI
     NcoI   NgoMIV     NspI    PflMI     PfoI    PpuMI     PstI    PvuII
     SacI    SanDI     SfcI     SfiI     SfoI     SmaI     SmlI     SphI
     SrfI     StuI     StyI    TaqII     TatI     TstI     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AlfI     AloI     ApaI    ApaLI     ApoI
     AscI     AseI    AsiSI    AvrII     BaeI     BbsI    BbvCI    BciVI
     BclI    BfrBI    BglII     BmrI     BmtI     BplI   Bpu10I     BsaI
    BsaAI    BsaBI    BsaWI    BsaXI    BsiWI    BsmBI    BspEI    BspHI
    BspMI    BsrDI    BsrGI   BssHII    BssSI    BstBI   BstEII    BstXI
  BstZ17I     ClaI    CspCI     DraI     DrdI    EcoRI    EcoRV     FalI
    FspAI    Hin4I   HincII  HindIII     HpaI     KpnI     MfeI     MluI
     MscI     NdeI     NheI     NotI     NruI     NsiI     PacI     PasI
     PciI     PmeI     PmlI     PpiI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PvuI    RsrII    SacII     SalI     SapI     SbfI     ScaI
    SexAI     SgfI    SgrAI    SnaBI     SpeI     SspI     SwaI  Tth111I
     XbaI     XhoI     XmnI     ZraI
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