Restriction map for pF1KE0228
##################################
# NO CUT ENZYMES (  196 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AciI        AclI        AcyI        AflII       AflIII      
AgeI        AhdI        Alw44I      AlwI        AlwNI       
AocI        Aor51HI     ApaI        ApaLI       AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AspHI       AviII       AvrII       BalI        BamHI       
BanI        BbeI        BbrPI       BbvCI       BbvI        
BcgI        BciVI       BclI        BcnI        BfaI        
BfrI        BglI        BglII       BlnI        BsaAI       
BsaBI       BsaHI       BsaI        BsaJI       BsaWI       
BseAI       BseRI       BsgI        BsiEI       BsiHKAI     
BsiWI       BsmBI       BsmI        Bsp1407I    BspDI       
BspEI       BspHI       BspLU11I    BsrBI       BsrDI       
BsrFI       BsrGI       BssHII      BssSI       Bst1107I    
BstAPI      BstBI       BstEII      BstF5I      BstPI       
BstUI       BstXI       BstYI       BstZ17I     Bsu36I      
CfoI        Cfr10I      ClaI        CpoI        DpnII       
DraI        DraIII      DrdI        DsaI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      Eco81I      
EcoNI       EcoO65I     EcoRI       EcoRV       EcoT14I     
EcoT22I     FauI        FbaI        Fnu4HI      FokI        
FseI        FspI        HaeII       HapII       HhaI        
Hin1I       HinP1I      HincII      HindII      HindIII     
HpaI        HpaII       ItaI        KasI        KpnI        
KspI        MaeI        MaeII       MamI        MboI        
MfeI        MflI        MluI        MluNI       MnlI        
MroI        MscI        MseI        MslI        MspA1I      
MspI        MunI        MvnI        NaeI        NarI        
NciI        NcoI        NdeI        NdeII       NgoMIV      
NheI        NotI        NruI        NsiI        NspV        
PacI        PaeR7I      PinAI       PmaCI       PmeI        
PmlI        PshAI       PshBI       Psp1406I    PstI        
PvuI        PvuII       RcaI        RsrII       SacI        
SacII       SalI        SapI        Sau3AI      SbfI        
SexAI       SfiI        SfoI        SfuI        SgrAI       
SmaI        SmlI        SnaBI       SpeI        Sse8387I    
SspBI       SspI        StuI        StyI        SwaI        
TaqI        Tru9I       TseI        TthHB8I     XbaI        
XcmI        XhoI        XhoII       XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   40 enzymes) #
###################################

AcsI          209  AAATTT               [GA]^AATT[CT]
AluI          165  AGCT                 AG^CT
ApoI          209  AAATTT               [GA]^AATT[CT]
AspI            7  GACAAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaI          161  CCCGAG               C^[CT]CG[GA]G
BanII         194  GGGCTC               G[GA]GC[CT]^C
BbsI          235  GAAGAC               GAAGAC(2/6)
BlpI          102  GCTGAGC              GC^T[ACGT]AGC
BmyI          194  GGGCTC               G[AGT]GC[ACT]^C
BpmI          197  CTCCAG               CTGGAG(16/14)
Bpu1102I      102  GCTGAGC              GC^T[ACGT]AGC
BpuAI         235  GAAGAC               GAAGAC(2/6)
BsmAI          91  GAGAC                GTCTC(1/5)
BsoBI         161  CCCGAG               C^[CT]CG[GA]G
Bsp1286I      194  GGGCTC               G[AGT]GC[ACT]^C
BspMI         271  GCAGGT               ACCTGC(4/8)
BsrI           75  ACTGG                ACTGG(1/-1)
Cac8I         202  GCATGC               GC[ACGT]^[ACGT]GC
CelII         102  GCTGAGC              GC^T[ACGT]AGC
DraII          11  AGGTCCT              [GA]G^G[ACGT]CC[CT]
EaeI            2  TGGCCG               [CT]^GGCC[GA]
EcoO109I       11  AGGTCCT              [GA]G^G[ACGT]CC[CT]
HgaI          255  GACGC                GACGC(5/10)
MaeIII        242  GTCAC                ^GT[ACGT]AC
MwoI          206  GCCAAATTTGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NlaIII        203  CATG                 CATG^
NlaIV         195  GGCTCC               GG[ACGT]^[ACGT]CC
NspI          202  GCATGC               [GA]CATG^[CT]
PflFI           7  GACAAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI         277  CCAACATCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI          11  AGGTCCT              [GA]G^G[AT]CC[CT]
ScaI          318  AGTACT               AGT^ACT
SfaNI         215  GCATC                GCATC(5/9)
SfcI          143  CTACAG               C^T[GA][CT]AG
SphI          202  GCATGC               GCATG^C
TfiI          307  GATTC                G^A[AT]TC
Tsp45I        242  GTCAC                ^GT[GC]AC
TspRI          47  CAGTG                CA[GC]TG[ACGT][ACGT]^
Tth111I         7  GACAAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Van91I        277  CCAACATCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG


###################################
# TWO CUT ENZYMES (   17 enzymes) #
###################################

AfaI           59  GTAC                 GT^AC
              319  GTAC                
AvaII          12  GGTCC                G^G[AT]CC
              274  GGTCC               
BsiYI          42  CCGTTCAGTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              277  CCAACATCTGG         
BslI           42  CCGTTCAGTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              277  CCAACATCTGG         
BsmFI         253  GGGAC                GGGAC(10/14)
              261  GGGAC               
BssKI         108  CCAGG                ^CC[ACGT]GG
              246  CCTGG               
BstNI         108  CCAGG                CC^[AT]GG
              246  CCTGG               
DdeI          103  CTGAG                C^T[ACGT]AG
              267  CTCAG               
EarI           24  GAAGAG               CTCTTC(1/4)
              112  GAAGAG              
EcoRII        108  CCAGG                ^CC[AT]GG
              246  CCTGG               
HaeIII          3  GGCC                 GG^CC
              159  GGCC                
Ksp632I        24  GAAGAG               CTCTTC(1/4)
              112  GAAGAG              
MvaI          108  CCAGG                CC^[AT]GG
              246  CCTGG               
PleI          175  GACTC                GAGTC(4/5)
              263  GACTC               
RsaI           59  GTAC                 GT^AC
              319  GTAC                
ScrFI         108  CCAGG                CC^[ACGT]GG
              246  CCTGG               
Tsp509I        83  AATT                 ^AATT
              210  AATT                


#########################################
# 3 OR MORE CUT ENZYMES (    5 enzymes) #
#########################################

Cfr13I      HinfI       HphI        MboII       Sau96I      
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