Restriction map for pF1KE0228
 (Circular) (Six-base) MAPSORT of: pF1KE0228.seq  Check: 9805  from: 1  to: 3443



pF1KE0228  3443 bp

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:12  ..

AccI GT'mk_AC

Cuts at:    481     481 
   Size:       3443
 
Acc65I G'GTAC_C

Cuts at:    459     459 
   Size:       3443
 
AclI AA'CG_TT

Cuts at:   3283    3283 
   Size:       3443
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    145    1268    1700    2447    2715     145 
   Size:       1123     432     747     268     873

  Fragments arranged by size: 

                1123     873     747     432     268
 
AflIII A'CryG_T

Cuts at:    795    1899    2876     795 
   Size:       1104     977    1362

  Fragments arranged by size: 

                1362    1104     977
 
AgeI A'CCGG_T

Cuts at:   1831    1831 
   Size:       3443
 
AlwNI CAG_nnn'CTG

Cuts at:    748    2315     748 
   Size:       1567    1876
 
ApaLI G'TGCA_C

Cuts at:   2213    2213 
   Size:       3443
 
ApoI r'AATT_y

Cuts at:    317     447    2796    3052     317 
   Size:        130    2349     256     708

  Fragments arranged by size: 

                2349     708     256     130
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       3443
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3443
 
AvaI C'yCGr_G

Cuts at:    269     463     269 
   Size:        194    3249
 
BamHI G'GATC_C

Cuts at:    468     468 
   Size:       3443
 
BanI G'GyrC_C

Cuts at:    459     873    1121    1156     459 
   Size:        414     248      35    2746

  Fragments arranged by size: 

                2746     414     248      35
 
BanII G_rGCy'C

Cuts at:    306     457    1487     306 
   Size:        151    1030    2262

  Fragments arranged by size: 

                2262    1030     151
 
BbeI G_GCGC'C

Cuts at:    877    1125     877 
   Size:        248    3195
 
BbsI GAAGACnn'nnnn_

Cuts at:    350     350 
   Size:       3443
 
BciVI GTATCCnnnnn_n'

Cuts at:   1341    2102    3443    1341 
   Size:        761    1341    1341

  Fragments arranged by size: 

                1341    1341     761
 
BclI T'GATC_A

Cuts at:    963     963 
   Size:       3443
 
BglII A'GATC_T

Cuts at:    958    2995     958 
   Size:       2037    1406
 
BlpI GC'TnA_GC

Cuts at:    211     555     211 
   Size:        344    3099
 
Bme1580I G_kGCm'C

Cuts at:   1068    1161    2217    1068 
   Size:         93    1056    2294

  Fragments arranged by size: 

                2294    1056      93
 
BmrI ACTGGGnnnn_n'

Cuts at:    193     655     736    1071    3215     193 
   Size:        462      81     335    2144     421

  Fragments arranged by size: 

                2144     462     421     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   1810    1810 
   Size:       3443
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    290     290 
   Size:       3443
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    619    1498    1990    2288     619 
   Size:        879     492     298    1774

  Fragments arranged by size: 

                1774     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   3147    3147 
   Size:       3443
 
BsaAI yAC'GTr

Cuts at:    796     796 
   Size:       3443
 
BsaBI GATnn'nnATC

Cuts at:      6     957    2994       6 
   Size:        951    2037     455

  Fragments arranged by size: 

                2037     951     455
 
BsaHI Gr'CG_yC

Cuts at:    874    1122    1824    3320     874 
   Size:        248     702    1496     997

  Fragments arranged by size: 

                1496     997     702     248
 
BsaWI w'CCGG_w

Cuts at:    632    1153    1831    2105    2252    2663     632 
   Size:        521     678     274     147     411    1412

  Fragments arranged by size: 

                1412     678     521     411     274     147
 
BseYI C'CCAG_C

Cuts at:    366     868    1626    2203    2699     366 
   Size:        502     758     577     496    1110

  Fragments arranged by size: 

                1110     758     577     502     496
 
BsiEI CG_ry'CG

Cuts at:    104     985    1031    2239     104 
   Size:        881      46    1208    1308

  Fragments arranged by size: 

                1308    1208     881      46
 
BsiHKAI G_wGCw'C

Cuts at:    457    1235    1425    2217     457 
   Size:        778     190     792    1683

  Fragments arranged by size: 

                1683     792     778     190
 
Bsp1286I G_dGCh'C

Cuts at:    306     457    1068    1161    1235    1425    1487    2217     306 
   Size:        151     611      93      74     190      62     730    1532

  Fragments arranged by size: 

                1532     730     611     190     151      93      74      62
            
 
BspEI T'CCGG_A

Cuts at:   2663    2663 
   Size:       3443
 
BspHI T'CATG_A

Cuts at:   2619    2619 
   Size:       3443
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    370     493     707    1009    1390     370 
   Size:        123     214     302     381    2423

  Fragments arranged by size: 

                2423     381     302     214     123
 
BsrBI CCG'CTC

Cuts at:     50    1735    1789    3273    3438      50 
   Size:       1685      54    1484     165      55

  Fragments arranged by size: 

                1685    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   1355    1355 
   Size:       3443
 
BsrFI r'CCGG_y

Cuts at:   1441    1622    1831    1441 
   Size:        181     209    3053

  Fragments arranged by size: 

                3053     209     181
 
BssSI C'ACGA_G

Cuts at:   1714    2072    1714 
   Size:        358    3085
 
BstAPI GCAn_nnn'nTGC

Cuts at:    820     820 
   Size:       3443
 
BstBI TT'CG_AA

Cuts at:   1804    1804 
   Size:       3443
 
BstYI r'GATC_y

Cuts at:      1     468     935     958    1293    1539    2551    2637    2649 
   Size:        467     467      23     335     246    1012      86      12

Cuts at:   2649    2995       1 
   Size:        346     449

  Fragments arranged by size: 

                1012     467     467     449     346     335     246      86
                  23      12
 
BtgI C'CryG_G

Cuts at:    107     107 
   Size:       3443
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   1393    1574    1737    1393 
   Size:        181     163    3099

  Fragments arranged by size: 

                3099     181     163
 
BtsI GCAGTG_nn'

Cuts at:    686     686 
   Size:       3443
 
DraI TTT'AAA

Cuts at:    442     442 
   Size:       3443
 
DrdI GACnn_nn'nnGTC

Cuts at:   1149    2007    1149 
   Size:        858    2585
 
EaeI y'GGCC_r

Cuts at:    110    1028    1202    1593    1620    2965     110 
   Size:        918     174     391      27    1345     588

  Fragments arranged by size: 

                1345     918     588     391     174      27
 
EagI C'GGCC_G

Cuts at:   1028    1028 
   Size:       3443
 
EarI CTCTTCn'nnn_

Cuts at:    127     215    1466    1676     127 
   Size:         88    1251     210    1894

  Fragments arranged by size: 

                1894    1251     210      88
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   1651    1961    2107    3253    1651 
   Size:        310     146    1146    1841

  Fragments arranged by size: 

                1841    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:    455     455 
   Size:       3443
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    145     290    1268    1700    2447    2715     145 
   Size:        145     978     432     747     268     873

  Fragments arranged by size: 

                 978     873     747     432     268     145
 
EcoO109I rG'GnC_Cy

Cuts at:    120     582     120 
   Size:        462    2981
 
EcoRI G'AATT_C

Cuts at:    447     447 
   Size:       3443
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   1462    1462 
   Size:       3443
 
FspI TGC'GCA

Cuts at:   1224    1224 
   Size:       3443
 
HaeII r_GCGC'y

Cuts at:    877    1125    2147    3126     877 
   Size:        248    1022     979    1194

  Fragments arranged by size: 

                1194    1022     979     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1285    1315    1317    1347    1285 
   Size:         30       2      30    3381

  Fragments arranged by size: 

                3381      30      30       2
 
HincII GTy'rAC

Cuts at:    482     482 
   Size:       3443
 
KasI G'GCGC_C

Cuts at:    873    1121     873 
   Size:        248    3195
 
KpnI G_GTAC'C

Cuts at:    463     463 
   Size:       3443
 
MluI A'CGCG_T

Cuts at:   2876    2876 
   Size:       3443
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:    409    2114    2298     409 
   Size:       1705     184    1554

  Fragments arranged by size: 

                1705    1554     184
 
MscI TGG'CCA

Cuts at:   1204    1204 
   Size:       3443
 
MslI CAynn'nnrTG

Cuts at:   1432    1559    1432 
   Size:        127    3316
 
MspA1I CmG'CkG

Cuts at:    554     868    1228    2241    2486     554 
   Size:        314     360    1013     245    1511

  Fragments arranged by size: 

                1511    1013     360     314     245
 
NaeI GCC'GGC

Cuts at:   1624    1624 
   Size:       3443
 
NarI GG'CG_CC

Cuts at:    874    1122     874 
   Size:        248    3195
 
NcoI C'CATG_G

Cuts at:    107     107 
   Size:       3443
 
NgoMIV G'CCGG_C

Cuts at:   1622    1622 
   Size:       3443
 
NspI r_CATG'y

Cuts at:    314     496     996    1903     314 
   Size:        182     500     907    1854

  Fragments arranged by size: 

                1854     907     500     182
 
PciI A'CATG_T

Cuts at:   1899    1899 
   Size:       3443
 
PflMI CCAn_nnn'nTGG

Cuts at:    391     391 
   Size:       3443
 
PmeI GTTT'AAAC

Cuts at:    442     442 
   Size:       3443
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2582    2614    2582 
   Size:         32    3411
 
PpuMI rG'GwC_Cy

Cuts at:    120     120 
   Size:       3443
 
PstI C_TGCA'G

Cuts at:    490    1175     490 
   Size:        685    2758
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       3443
 
PvuII CAG'CTG

Cuts at:    868    1228     868 
   Size:        360    3083
 
RsrII CG'GwC_CG

Cuts at:   1638    1638 
   Size:       3443
 
SacI G_AGCT'C

Cuts at:    457     457 
   Size:       3443
 
SalI G'TCGA_C

Cuts at:    480     480 
   Size:       3443
 
SapI GCTCTTCn'nnn_

Cuts at:   1466    1676    1466 
   Size:        210    3233
 
SbfI CC_TGCA'GG

Cuts at:    490     490 
   Size:       3443
 
ScaI AGT'ACT

Cuts at:    428    2960     428 
   Size:       2532     911
 
SfcI C'TryA_G

Cuts at:     33     251     486    1171    2164    2355      33 
   Size:        218     235     685     993     191    1121

  Fragments arranged by size: 

                1121     993     685     235     218     191
 
SfoI GGC'GCC

Cuts at:    875    1123     875 
   Size:        248    3195
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3443
 
SmaI CCC'GGG

Cuts at:    465     465 
   Size:       3443
 
SmlI C'TyrA_G

Cuts at:    598    1513    2005    2267     598 
   Size:        915     492     262    1774

  Fragments arranged by size: 

                1774     915     492     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       3443
 
SphI G_CATG'C

Cuts at:    314     496     996     314 
   Size:        182     500    2761

  Fragments arranged by size: 

                2761     500     182
 
StyI C'CwwG_G

Cuts at:    107     577     931     107 
   Size:        470     354    2619

  Fragments arranged by size: 

                2619     470     354
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    175    1026    1159    1618    1827     175 
   Size:        851     133     459     209    1791

  Fragments arranged by size: 

                1791     851     459     209     133
 
TatI w'GTAC_w

Cuts at:    426    2958     426 
   Size:       2532     911
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   2960    2992    2960 
   Size:         32    3411
 
Tth111I GACn'n_nGTC

Cuts at:    118    1240     118 
   Size:       1122    2321
 
XbaI T'CTAG_A

Cuts at:    474     474 
   Size:       3443
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:    938     938 
   Size:       3443
 
XmaI C'CCGG_G

Cuts at:    463     463 
   Size:       3443
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI   AflIII     AgeI    AlwNI    ApaLI
     ApoI     AseI    AsiSI     AvaI    BamHI     BanI    BanII     BbeI
     BbsI    BciVI     BclI    BglII     BlpI Bme1580I     BmrI     BplI
     BpmI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BseYI
    BsiEI  BsiHKAI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI
    BsrFI    BssSI   BstAPI    BstBI    BstYI     BtgI    BtgZI     BtsI
     DraI     DrdI     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI
 EcoO109I    EcoRI     FalI     FspI    HaeII    Hin4I   HincII     KasI
     KpnI     MluI     MmeI     MscI     MslI   MspA1I     NaeI     NarI
     NcoI   NgoMIV     NspI     PciI    PflMI     PmeI     PpiI    PpuMI
     PstI     PvuI    PvuII    RsrII     SacI     SalI     SapI     SbfI
     ScaI     SfcI     SfoI     SgfI     SmaI     SmlI     SpeI     SphI
     StyI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AfeI    AflII     AhdI     AleI     AlfI     AloI
     ApaI     AscI    AvrII     BaeI    BbvCI     BcgI    BfrBI     BglI
    BmgBI     BmtI   Bpu10I    BsaXI    BseRI     BsgI    BsiWI     BsmI
    BsmBI    BsrGI   BssHII   BstEII    BstXI  BstZ17I   Bsu36I     ClaI
    CspCI   DraIII    EcoNI    EcoRV     FseI    FspAI  HindIII     HpaI
     MfeI     NdeI     NheI     NotI     NruI     NsiI     PacI     PasI
     PfoI     PmlI    PshAI     PsiI   PspOMI    PspXI     PsrI    SacII
    SanDI    SexAI     SfiI    SgrAI    SnaBI     SrfI     SspI     StuI
     SwaI     XhoI     XmnI     ZraI
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