Restriction map for pF1KB6543
##################################
# NO CUT ENZYMES (  158 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AflII       AgeI        AhdI        Alw44I      
AocI        Aor51HI     ApaLI       AscI        AseI        
AsnI        Asp718I     AspEI       AspI        AvaI        
AviII       AvrII       BalI        BamHI       BanI        
BbeI        BbrPI       BbvCI       BcgI        BciVI       
BclI        BcnI        BfrI        BglI        BglII       
BlnI        BsaAI       BsaBI       BsaI        BseAI       
BseRI       BsiEI       BsiWI       BsmBI       BsmI        
BsoBI       Bsp1407I    BspDI       BspEI       BspHI       
BspLU11I    BspMI       BsrBI       BsrDI       BsrFI       
BsrGI       BssHII      BssSI       Bst1107I    BstBI       
BstEII      BstPI       BstUI       BstZ17I     Bsu36I      
Cac8I       CfoI        Cfr10I      ClaI        CpoI        
DraI        DrdI        EaeI        EagI        Eam1105I    
EarI        EclXI       Eco47III    Eco52I      Eco81I      
EcoO65I     EcoRV       EcoT22I     FauI        FbaI        
FseI        FspI        HaeII       HhaI        HinP1I      
HincII      HindII      HindIII     HpaI        KasI        
KpnI        Ksp632I     KspI        MamI        MfeI        
MluI        MluNI       MroI        MscI        MunI        
MvnI        NaeI        NarI        NciI        NdeI        
NgoMIV      NheI        NotI        NruI        NsiI        
NspI        NspV        PacI        PaeR7I      PflFI       
PinAI       PmaCI       PmeI        PmlI        PshAI       
PshBI       Psp1406I    PvuI        RcaI        RsrII       
SacI        SacII       SalI        SapI        SbfI        
ScaI        SexAI       SfiI        SfoI        SfuI        
SgrAI       SmaI        SmlI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        StuI        
SwaI        Tsp45I      Tth111I     XbaI        XcmI        
XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   32 enzymes) #
###################################

AciI          675  CCGC                 CCGC(-3/-1)
AcsI          866  GAATTC               [GA]^AATT[CT]
AcyI           84  GACGCC               G[GA]^CG[CT]C
AflIII        656  ACACGT               A^C[GA][CT]GT
AlwNI         445  CAGAATCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApoI          866  GAATTC               [GA]^AATT[CT]
Asp700I       759  GAATCCTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BbsI          100  GAAGAC               GAAGAC(2/6)
BlpI          700  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I      700  GCTCAGC              GC^T[ACGT]AGC
BpuAI         100  GAAGAC               GAAGAC(2/6)
BsaHI          84  GACGCC               G[GA]^CG[CT]C
BsaWI         566  ACCGGA               [AT]^CCGG[AT]
BsmAI         417  GAGAC                GTCTC(1/5)
BstAPI        552  GCACTTGGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI         727  CCAATCCACTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII         700  GCTCAGC              GC^T[ACGT]AGC
EcoRI         866  GAATTC               G^AATTC
HapII         567  CCGG                 C^CGG
HgaI           84  GACGC                GACGC(5/10)
Hin1I          84  GACGCC               G[GA]^CG[CT]C
HpaII         567  CCGG                 C^CGG
HphI          453  GGTGA                GGTGA(8/7)
MaeII         658  ACGT                 A^CGT
MseI          680  TTAA                 T^TAA
MspI          567  CCGG                 C^CGG
NcoI          806  CCATGG               C^CATGG
PpuMI         398  AGGACCC              [GA]G^G[AT]CC[CT]
TaqI          200  TCGA                 T^CGA
Tru9I         680  TTAA                 T^TAA
TthHB8I       200  TCGA                 T^CGA
XmnI          759  GAATCCTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   20 enzymes) #
###################################

ApaI         1019  GGGCCC               GGGCC^C
             1073  GGGCCC              
AspHI         550  GAGCAC               G[AT]GC[AT]^C
              698  GTGCTC              
AvaII         399  GGACC                G^G[AT]CC
              579  GGACC               
BsgI          303  GTGCAG               GTGCAG(16/14)
              559  GTGCAG              
BsiHKAI       550  GAGCAC               G[AT]GC[AT]^C
              698  GTGCTC              
BsmFI         578  GGGAC                GGGAC(10/14)
              945  GGGAC               
BstF5I         26  CATCC                GGATG(2/0)
              639  GGATG               
BstYI         362  AGATCC               [GA]^GATC[CT]
              891  GGATCT              
DraIII        553  CACTTGGTG            CAC[ACGT][ACGT][ACGT]^GTG
              686  CACTCTGTG           
EcoNI         168  CCTCCCAGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             1023  CCTTGGGGAGG         
EcoT14I       806  CCATGG               C^C[AT][AT]GG
             1023  CCTTGG              
FokI           26  CATCC                GGATG(9/13)
              639  GGATG               
MaeIII        898  GTTAC                ^GT[ACGT]AC
             1085  GTTAC               
MflI          362  AGATCC               [GA]^GATC[CT]
              891  GGATCT              
MslI          426  CACACAGATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              706  CATTGGAGTG          
PflMI         540  CCACTGTTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              685  CCACTCTGTGG         
SfaNI         432  GATGC                GCATC(5/9)
              858  GATGC               
StyI          806  CCATGG               C^C[AT][AT]GG
             1023  CCTTGG              
Van91I        540  CCACTGTTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              685  CCACTCTGTGG         
XhoII         362  AGATCC               [GA]^GATC[CT]
              891  GGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (   48 enzymes) #
#########################################

AfaI        AluI        AlwI        BanII       BbvI        
BfaI        BmyI        BpmI        BsaJI       BsiYI       
BslI        Bsp1286I    BsrI        BssKI       BstNI       
Cfr13I      DdeI        DpnII       DraII       DsaI        
EcoO109I    EcoRII      Fnu4HI      HaeIII      HinfI       
ItaI        MaeI        MboI        MboII       MnlI        
MspA1I      MvaI        MwoI        NdeII       NlaIII      
NlaIV       PleI        PstI        PvuII       RsaI        
Sau3AI      Sau96I      ScrFI       SfcI        TfiI        
TseI        Tsp509I     TspRI       
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