Restriction map for pF1KF0190
##################################
# NO CUT ENZYMES (  121 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AflII       
AgeI        AhdI        Alw44I      ApaLI       AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AspI        AviII       AvrII       BamHI       BbeI        
BbrPI       BbvCI       BcgI        BciVI       BclI        
BfaI        BfrI        BglII       BlnI        BlpI        
Bpu1102I    BsaBI       BsaI        BseAI       BsgI        
BsiEI       BsiWI       BsmAI       BsmBI       BspDI       
BspEI       BspHI       BspLU11I    BsrDI       BssHII      
BssSI       Bst1107I    BstAPI      BstBI       BstEII      
BstPI       BstZ17I     CelII       ClaI        CpoI        
DraI        DrdI        EagI        Eam1105I    EclXI       
Eco52I      EcoO65I     EcoRV       EcoT22I     FbaI        
FseI        FspI        HincII      HindII      HindIII     
HpaI        KasI        KpnI        KspI        MaeI        
MamI        MfeI        MroI        MseI        MslI        
MunI        NarI        NdeI        NheI        NotI        
NruI        NsiI        NspV        PacI        PflFI       
PinAI       PmaCI       PmeI        PmlI        PshBI       
Psp1406I    PvuI        RcaI        RsrII       SacII       
SalI        SbfI        ScaI        SexAI       SfiI        
SfoI        SfuI        SgrAI       SmaI        SnaBI       
SpeI        Sse8387I    SspI        StuI        SwaI        
Tru9I       Tth111I     XbaI        XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   45 enzymes) #
###################################

AccI          194  GTAGAC               GT^[AC][GT]AC
AcsI         1285  GAATTC               [GA]^AATT[CT]
AocI         1438  CCTGAGG              CC^T[ACGT]AGG
Aor51HI        14  AGCGCT               AGC^GCT
ApoI         1285  GAATTC               [GA]^AATT[CT]
BbsI          747  GAAGAC               GAAGAC(2/6)
BcnI         1246  CCGGG                CC^[GC]GG
BglI          673  GCCAACTGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI         747  GAAGAC               GAAGAC(2/6)
BsaAI         429  CACGTA               [CT]AC^GT[GA]
BsaWI        1365  ACCGGA               [AT]^CCGG[AT]
BsmI          481  GCATTC               GAATGC(1/-1)
Bsp1407I      275  TGTACA               T^GTACA
BspMI         646  ACCTGC               ACCTGC(4/8)
BsrBI          50  CCGCTC               CCGCTC(-3/-3)
BsrFI         372  GCCGGC               [GA]^CCGG[CT]
BsrGI         275  TGTACA               T^GTACA
BstXI        1172  CCAGCACTGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I       1438  CCTGAGG              CC^T[ACGT]AGG
Cfr10I        372  GCCGGC               [GA]^CCGG[CT]
DraIII        820  CACCCTGTG            CAC[ACGT][ACGT][ACGT]^GTG
Eco47III       14  AGCGCT               AGC^GCT
Eco81I       1438  CCTGAGG              CC^T[ACGT]AGG
EcoRI        1285  GAATTC               G^AATTC
HaeII          14  AGCGCT               [GA]GCGC^[CT]
HphI          395  TCACC                GGTGA(8/7)
MluI          294  ACGCGT               A^CGCGT
NaeI          372  GCCGGC               GCC^GGC
NciI         1246  CCGGG                CC^[GC]GG
NcoI          531  CCATGG               C^CATGG
NgoMIV        372  GCCGGC               G^CCGGC
NspI          449  GCATGC               [GA]CATG^[CT]
PaeR7I        917  CTCGAG               C^TCGAG
PflMI         133  CCAGGGCTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI         848  GACCAGAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI           43  CTGCAG               CTGCA^G
SapI         1312  GAAGAGC              GCTCTTC(1/4)
SfcI           43  CTGCAG               C^T[GA][CT]AG
SmlI          917  CTCGAG               C^T[CT][GA]AG
SphI          449  GCATGC               GCATG^C
SspBI         275  TGTACA               T^GTACA
TaqI          918  TCGA                 T^CGA
TthHB8I       918  TCGA                 T^CGA
Van91I        133  CCAGGGCTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI          917  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   23 enzymes) #
###################################

AccII         205  CGCG                 CG^CG
              295  CGCG                
AcyI          912  GACGCC               G[GA]^CG[CT]C
             1009  GGCGTC              
AflIII        294  ACGCGT               A^C[GA][CT]GT
              605  ACACGT              
AlwI          411  GATCC                GGATC(4/5)
              462  GGATC               
BalI          534  TGGCCA               TGG^CCA
             1553  TGGCCA              
BanI         1259  GGCACC               G^G[CT][GA]CC
             1354  GGCACC              
BsaHI         912  GACGCC               G[GA]^CG[CT]C
             1009  GGCGTC              
BstUI         205  CGCG                 CG^CG
              295  CGCG                
BstYI         410  AGATCC               [GA]^GATC[CT]
              462  GGATCT              
CfoI           15  GCGC                 GCG^C
              204  GCGC                
DsaI          531  CCATGG               C^C[GA][CT]GG
              790  CCACGG              
EaeI          534  TGGCCA               [CT]^GGCC[GA]
             1553  TGGCCA              
HhaI           15  GCGC                 GCG^C
              204  GCGC                
Hin1I         912  GACGCC               G[GA]^CG[CT]C
             1009  GGCGTC              
HinP1I         15  GCGC                 G^CGC
              204  GCGC                
MaeII         430  ACGT                 A^CGT
              607  ACGT                
MflI          410  AGATCC               [GA]^GATC[CT]
              462  GGATCT              
MluNI         534  TGGCCA               TGG^CCA
             1553  TGGCCA              
MscI          534  TGGCCA               TGG^CCA
             1553  TGGCCA              
MvnI          205  CGCG                 CG^CG
              295  CGCG                
SacI          180  GAGCTC               GAGCT^C
             1315  GAGCTC              
SfaNI         786  GATGC                GCATC(5/9)
             1483  GATGC               
XhoII         410  AGATCC               [GA]^GATC[CT]
              462  GGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (   69 enzymes) #
#########################################

AciI        AfaI        AluI        AlwNI       ApaI        
AspHI       AvaI        AvaII       BanII       BbvI        
BmyI        BpmI        BsaJI       BseRI       BsiHKAI     
BsiYI       BslI        BsmFI       BsoBI       Bsp1286I    
BsrI        BssKI       BstF5I      BstNI       Cac8I       
Cfr13I      DdeI        DpnII       DraII       EarI        
EcoNI       EcoO109I    EcoRII      EcoT14I     FauI        
Fnu4HI      FokI        HaeIII      HapII       HgaI        
HinfI       HpaII       ItaI        Ksp632I     MaeIII      
MboI        MboII       MnlI        MspA1I      MspI        
MvaI        MwoI        NdeII       NlaIII      NlaIV       
PleI        PpuMI       PvuII       RsaI        Sau3AI      
Sau96I      ScrFI       StyI        TfiI        TseI        
Tsp45I      Tsp509I     TspRI       XcmI        
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com