Restriction map for pF1KF0190
 (Linear) (Six-base) MAPSORT of: FLJ00190.seq  Check: 1741  from: 1  to: 1560



FLJ00190.orf

 With 182 enzymes: SgfI * 

                        July 18, 2019 20:57  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     655    1560 
   Size:        655     905
 
AccI GT'mk_AC

Cuts at:      0     195    1560 
   Size:        195    1365
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      93     280     487    1139    1390    1560 
   Size:         93     187     207     652     251     170

  Fragments arranged by size: 

                 652     251     207     187     170      93
 
AfeI AGC'GCT

Cuts at:      0      16    1560 
   Size:         16    1544
 
AflIII A'CryG_T

Cuts at:      0     294     605    1560 
   Size:        294     311     955

  Fragments arranged by size: 

                 955     311     294
 
AlwNI CAG_nnn'CTG

Cuts at:      0     273    1166    1413    1560 
   Size:        273     893     247     147

  Fragments arranged by size: 

                 893     273     247     147
 
ApaI G_GGCC'C

Cuts at:      0      11      25     798     930    1560 
   Size:         11      14     773     132     630

  Fragments arranged by size: 

                 773     630     132      14      11
 
ApoI r'AATT_y

Cuts at:      0    1285    1560 
   Size:       1285     275
 
AvaI C'yCGr_G

Cuts at:      0      10     917    1299    1560 
   Size:         10     907     382     261

  Fragments arranged by size: 

                 907     382     261      10
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     700     733    1560 
   Size:        700      33     827

  Fragments arranged by size: 

                 827     700      33
 
BanI G'GyrC_C

Cuts at:      0    1259    1354    1560 
   Size:       1259      95     206

  Fragments arranged by size: 

                1259     206      95
 
BanII G_rGCy'C

Cuts at:      0      11      25     184     798     930    1116    1319    1560 
   Size:         11      14     159     614     132     186     203     241

  Fragments arranged by size: 

                 614     241     203     186     159     132      14      11
            
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     754    1560 
   Size:        754     806
 
BglI GCCn_nnn'nGGC

Cuts at:      0     679    1560 
   Size:        679     881
 
Bme1580I G_kGCm'C

Cuts at:      0      11      25     798     930    1076    1262    1357    1560 
   Size:         11      14     773     132     146     186      95     203

  Fragments arranged by size: 

                 773     203     186     146     132      95      14      11
            
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     687    1390    1560 
   Size:        687     703     170

  Fragments arranged by size: 

                 703     687     170
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      38     541     679    1052    1560 
   Size:         38     503     138     373     508

  Fragments arranged by size: 

                 508     503     373     138      38
 
BsaAI yAC'GTr

Cuts at:      0     431    1560 
   Size:        431    1129
 
BsaHI Gr'CG_yC

Cuts at:      0     913    1010    1560 
   Size:        913      97     550

  Fragments arranged by size: 

                 913     550      97
 
BsaWI w'CCGG_w

Cuts at:      0    1365    1560 
   Size:       1365     195
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      51      81     294     324    1321    1351    1560 
   Size:         51      30     213      30     997      30     209

  Fragments arranged by size: 

                 997     213     209      51      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     421     994     997    1006    1106    1128    1176    1326 
   Size:        421     573       3       9     100      22      48     150

Cuts at:   1326    1456    1465    1560 
   Size:        130       9      95

  Fragments arranged by size: 

                 573     421     150     130     100      95      48      22
                   9       9       3
 
BseYI C'CCAG_C

Cuts at:      0      17     313     841     930    1166    1201    1424    1493 
   Size:         17     296     528      89     236      35     223      69

Cuts at:   1493    1560 
   Size:         67

  Fragments arranged by size: 

                 528     296     236     223      89      69      67      35
                  17
 
BsiHKAI G_wGCw'C

Cuts at:      0     184     395    1061    1319    1510    1560 
   Size:        184     211     666     258     191      50

  Fragments arranged by size: 

                 666     258     211     191     184      50
 
BsmI GAATG_Cn'

Cuts at:      0     481    1560 
   Size:        481    1079
 
Bsp1286I G_dGCh'C

Cuts at:      0      11      25     184     395     798     930    1061    1076 
   Size:         11      14     159     211     403     132     131      15

Cuts at:   1076    1116    1262    1319    1357    1510    1560 
   Size:         40     146      57      38     153      50

  Fragments arranged by size: 

                 403     211     159     153     146     132     131      57
                  50      40      38      15      14      11
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     655    1560 
   Size:        655     905
 
BsrBI CCG'CTC

Cuts at:      0      52    1560 
   Size:         52    1508
 
BsrFI r'CCGG_y

Cuts at:      0     372    1560 
   Size:        372    1188
 
BsrGI T'GTAC_A

Cuts at:      0     275    1560 
   Size:        275    1285
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1179    1560 
   Size:       1179     381
 
BstYI r'GATC_y

Cuts at:      0     410     462    1560 
   Size:        410      52    1098

  Fragments arranged by size: 

                1098     410      52
 
Bsu36I CC'TnA_GG

Cuts at:      0    1439    1560 
   Size:       1439     121
 
BtgI C'CryG_G

Cuts at:      0     531     790    1560 
   Size:        531     259     770

  Fragments arranged by size: 

                 770     531     259
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     683    1496    1560 
   Size:        683     813      64

  Fragments arranged by size: 

                 813     683      64
 
BtsI GCAGTG_nn'

Cuts at:      0     579    1560 
   Size:        579     981
 
DraIII CAC_nnn'GTG

Cuts at:      0     825    1560 
   Size:        825     735
 
EaeI y'GGCC_r

Cuts at:      0     534    1553    1560 
   Size:        534    1019       7

  Fragments arranged by size: 

                1019     534       7
 
EarI CTCTTCn'nnn_

Cuts at:      0     209     971    1307    1409    1442    1560 
   Size:        209     762     336     102      33     118

  Fragments arranged by size: 

                 762     336     209     118     102      33
 
EcoICRI GAG'CTC

Cuts at:      0     182    1317    1560 
   Size:        182    1135     243

  Fragments arranged by size: 

                1135     243     182
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      38      93     280     487     541     679    1052    1139 
   Size:         38      55     187     207      54     138     373      87

Cuts at:   1139    1390    1560 
   Size:        251     170

  Fragments arranged by size: 

                 373     251     207     187     170     138      87      55
                  54      38
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0      43     967    1194    1560 
   Size:         43     924     227     366

  Fragments arranged by size: 

                 924     366     227      43
 
EcoO109I rG'GnC_Cy

Cuts at:      0       7      21      22      62     270     663     795     926 
   Size:          7      14       1      40     208     393     132     131

Cuts at:    926     927     960    1205    1390    1560 
   Size:          1      33     245     185     170

  Fragments arranged by size: 

                 393     245     208     185     170     132     131      40
                  33      14       7       1       1
 
EcoRI G'AATT_C

Cuts at:      0    1285    1560 
   Size:       1285     275
 
HaeII r_GCGC'y

Cuts at:      0      18    1560 
   Size:         18    1542
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      51      83     294     326    1318    1350    1560 
   Size:         51      32     211      32     992      32     210

  Fragments arranged by size: 

                 992     211     210      51      32      32      32
 
MluI A'CGCG_T

Cuts at:      0     294    1560 
   Size:        294    1266
 
MscI TGG'CCA

Cuts at:      0     536    1555    1560 
   Size:        536    1019       5

  Fragments arranged by size: 

                1019     536       5
 
MspA1I CmG'CkG

Cuts at:      0     438     565     777    1493    1560 
   Size:        438     127     212     716      67

  Fragments arranged by size: 

                 716     438     212     127      67
 
NaeI GCC'GGC

Cuts at:      0     374    1560 
   Size:        374    1186
 
NcoI C'CATG_G

Cuts at:      0     531    1560 
   Size:        531    1029
 
NgoMIV G'CCGG_C

Cuts at:      0     372    1560 
   Size:        372    1188
 
NspI r_CATG'y

Cuts at:      0     453    1560 
   Size:        453    1107
 
PasI CC'CwG_GG

Cuts at:      0     133     329    1109    1384    1560 
   Size:        133     196     780     275     176

  Fragments arranged by size: 

                 780     275     196     176     133
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     139    1560 
   Size:        139    1421
 
PfoI T'CCnGG_A

Cuts at:      0    1307    1560 
   Size:       1307     253
 
PpuMI rG'GwC_Cy

Cuts at:      0     270     663     960    1205    1390    1560 
   Size:        270     393     297     245     185     170

  Fragments arranged by size: 

                 393     297     270     245     185     170
 
PshAI GACnn'nnGTC

Cuts at:      0     852    1560 
   Size:        852     708
 
PspOMI G'GGCC_C

Cuts at:      0       7      21     794     926    1560 
   Size:          7      14     773     132     634

  Fragments arranged by size: 

                 773     634     132      14       7
 
PspXI vC'TCGA_Gb

Cuts at:      0     917    1560 
   Size:        917     643
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     268     300    1560 
   Size:        268      32    1260

  Fragments arranged by size: 

                1260     268      32
 
PstI C_TGCA'G

Cuts at:      0      47    1560 
   Size:         47    1513
 
PvuII CAG'CTG

Cuts at:      0     438     565    1493    1560 
   Size:        438     127     928      67

  Fragments arranged by size: 

                 928     438     127      67
 
SacI G_AGCT'C

Cuts at:      0     184    1319    1560 
   Size:        184    1135     241

  Fragments arranged by size: 

                1135     241     184
 
SanDI GG'GwC_CC

Cuts at:      0    1390    1560 
   Size:       1390     170
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1307    1560 
   Size:       1307     253
 
SfcI C'TryA_G

Cuts at:      0      43    1560 
   Size:         43    1517
 
SmlI C'TyrA_G

Cuts at:      0     917    1560 
   Size:        917     643
 
SphI G_CATG'C

Cuts at:      0     453    1560 
   Size:        453    1107
 
StyI C'CwwG_G

Cuts at:      0     230     531    1239    1292    1560 
   Size:        230     301     708      53     268

  Fragments arranged by size: 

                 708     301     268     230      53
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     321     412     952    1525    1534    1560 
   Size:        321      91     540     573       9      26

  Fragments arranged by size: 

                 573     540     321      91      26       9
 
TatI w'GTAC_w

Cuts at:      0     275    1560 
   Size:        275    1285
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1028    1060    1560 
   Size:       1028      32     500

  Fragments arranged by size: 

                1028     500      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     674    1116    1246    1560 
   Size:        674     442     130     314

  Fragments arranged by size: 

                 674     442     314     130
 
XhoI C'TCGA_G

Cuts at:      0     917    1560 
   Size:        917     643
 

 Enzymes that do cut:

     AarI     AccI     AcuI     AfeI   AflIII    AlwNI     ApaI     ApoI
     AvaI     BaeI     BanI    BanII     BbsI     BglI Bme1580I     BmrI
     BpmI    BsaAI    BsaHI    BsaWI    BsaXI    BseRI    BseYI  BsiHKAI
     BsmI Bsp1286I    BspMI    BsrBI    BsrFI    BsrGI    BstXI    BstYI
   Bsu36I     BtgI    BtgZI     BtsI   DraIII     EaeI     EarI  EcoICRI
  Eco57MI    EcoNI EcoO109I    EcoRI    HaeII    Hin4I     MluI     MscI
   MspA1I     NaeI     NcoI   NgoMIV     NspI     PasI    PflMI     PfoI
    PpuMI    PshAI   PspOMI    PspXI     PsrI     PstI    PvuII     SacI
    SanDI     SapI     SfcI     SmlI     SphI     StyI    TaqII     TatI
     TstI     XcmI     XhoI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI    AflII     AgeI     AhdI     AleI     AlfI
     AloI    ApaLI     AscI     AseI    AsiSI    AvrII    BamHI     BbeI
    BbvCI     BcgI    BciVI     BclI    BfrBI    BglII     BlpI    BmgBI
     BmtI     BplI   Bpu10I    BpuEI     BsaI    BsaBI     BsgI    BsiEI
    BsiWI    BsmBI    BspEI    BspHI    BsrDI   BssHII    BssSI   BstAPI
    BstBI   BstEII  BstZ17I     ClaI    CspCI     DraI     DrdI     EagI
     EciI    EcoRV     FalI     FseI     FspI    FspAI   HincII  HindIII
     HpaI     KasI     KpnI     MfeI     MmeI     MslI     NarI     NdeI
     NheI     NotI     NruI     NsiI     PacI     PciI     PmeI     PmlI
     PpiI     PsiI     PvuI    RsrII    SacII     SalI     SbfI     ScaI
    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI
     SrfI     SspI     StuI     SwaI  Tth111I     XbaI     XmaI     XmnI
     ZraI
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