Restriction map for pF1KB6367
##################################
# NO CUT ENZYMES (  161 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcyI        AflII       AflIII      AgeI        AhdI        
AocI        Aor51HI     ApaI        AscI        AseI        
AsnI        Asp700I     Asp718I     AspEI       AspI        
AvaI        AviII       AvrII       BamHI       BanI        
BbeI        BbrPI       BbvI        BcgI        BciVI       
BclI        BfaI        BfrI        BglI        BglII       
BlnI        BlpI        Bpu1102I    BsaAI       BsaHI       
BsaI        BsaWI       BseAI       BseRI       BsiEI       
BsiWI       BsmAI       BsmBI       BsmI        BsoBI       
BspDI       BspEI       BspHI       BspLU11I    BspMI       
BsrBI       BsrFI       BssHII      BssSI       Bst1107I    
BstAPI      BstBI       BstEII      BstPI       BstXI       
BstZ17I     Bsu36I      CelII       CfoI        Cfr10I      
ClaI        CpoI        DraI        DraIII      DrdI        
EagI        Eam1105I    EarI        EclXI       Eco47III    
Eco52I      Eco81I      EcoNI       EcoO65I     EcoRI       
EcoRV       EcoT22I     FauI        FbaI        FseI        
FspI        HaeII       HhaI        Hin1I       HinP1I      
HincII      HindII      HpaI        KasI        KpnI        
Ksp632I     KspI        MaeI        MaeII       MaeIII      
MfeI        MluI        MroI        MseI        MslI        
MunI        MwoI        NaeI        NarI        NgoMIV      
NheI        NotI        NruI        NsiI        NspV        
PacI        PaeR7I      PflFI       PinAI       PmaCI       
PmeI        PmlI        PshAI       PshBI       Psp1406I    
PstI        PvuI        RcaI        RsrII       SacII       
SalI        SapI        SbfI        ScaI        SexAI       
SfiI        SfoI        SfuI        SgrAI       SmaI        
SnaBI       SpeI        SphI        Sse8387I    SspI        
StuI        SwaI        Tru9I       TseI        Tsp45I      
Tth111I     XbaI        XhoI        XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   45 enzymes) #
###################################

AccII         192  CGCG                 CG^CG
AcsI          679  GAATTT               [GA]^AATT[CT]
Alw44I        340  GTGCAC               G^TGCAC
AlwI          604  GATCC                GGATC(4/5)
AlwNI         109  CAGGACCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApaLI         340  GTGCAC               G^TGCAC
ApoI          679  GAATTT               [GA]^AATT[CT]
BalI          206  TGGCCA               TGG^CCA
BanII          16  GAGCTC               G[GA]GC[CT]^C
BbvCI         219  GCTGAGG              CCTCAGC(-5/-2)
BcnI          378  CCGGG                CC^[GC]GG
BsaBI         657  GATAGAAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsgI          369  CTGCAC               GTGCAG(16/14)
Bsp1407I      486  TGTACA               T^GTACA
BsrDI          50  GCAATG               GCAATG(2/0)
BsrGI         486  TGTACA               T^GTACA
BstUI         192  CGCG                 CG^CG
BstYI         603  AGATCC               [GA]^GATC[CT]
DraII         110  AGGACCT              [GA]G^G[ACGT]CC[CT]
DsaI          209  CCATGG               C^C[GA][CT]GG
EcoO109I      110  AGGACCT              [GA]G^G[ACGT]CC[CT]
HapII         378  CCGG                 C^CGG
HgaI          193  GCGTC                GACGC(5/10)
HindIII       396  AAGCTT               A^AGCTT
HpaII         378  CCGG                 C^CGG
MamI          657  GATAGAAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI          603  AGATCC               [GA]^GATC[CT]
MluNI         206  TGGCCA               TGG^CCA
MscI          206  TGGCCA               TGG^CCA
MspA1I        589  CAGCTG               C[AC]G^C[GT]G
MspI          378  CCGG                 C^CGG
MvnI          192  CGCG                 CG^CG
NciI          378  CCGGG                CC^[GC]GG
NcoI          209  CCATGG               C^CATGG
NdeI          416  CATATG               CA^TATG
NspI          734  ACATGC               [GA]CATG^[CT]
PflMI         108  CCAGGACCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI         110  AGGACCT              [GA]G^G[AT]CC[CT]
PvuII         589  CAGCTG               CAG^CTG
SacI           16  GAGCTC               GAGCT^C
SspBI         486  TGTACA               T^GTACA
TaqI          314  TCGA                 T^CGA
TthHB8I       314  TCGA                 T^CGA
Van91I        108  CCAGGACCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII         603  AGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   15 enzymes) #
###################################

BpmI          439  CTCCAG               CTGGAG(16/14)
              592  CTGGAG              
DpnII         359  GATC                 ^GATC
              604  GATC                
EaeI          206  TGGCCA               [CT]^GGCC[GA]
              765  CGGCCA              
EcoT14I       209  CCATGG               C^C[AT][AT]GG
              524  CCTTGG              
HphI          402  GGTGA                GGTGA(8/7)
              785  TCACC               
MboI          359  GATC                 ^GATC
              604  GATC                
NdeII         359  GATC                 ^GATC
              604  GATC                
NlaIV         234  GGTTCC               GG[ACGT]^[ACGT]CC
              754  GGGACC              
PleI          382  GACTC                GAGTC(4/5)
              685  GAGTC               
Sau3AI        359  GATC                 ^GATC
              604  GATC                
SfcI          168  CTACAG               C^T[GA][CT]AG
              586  CTACAG              
SmlI          532  CTCAAG               C^T[CT][GA]AG
              722  CTCAAG              
StyI          209  CCATGG               C^C[AT][AT]GG
              524  CCTTGG              
TfiI          152  GAATC                G^A[AT]TC
              543  GAATC               
XcmI           23  CCAGTGAAATGATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
              581  CCACTCTACAGCTGG     


#########################################
# 3 OR MORE CUT ENZYMES (   37 enzymes) #
#########################################

AciI        AfaI        AluI        AspHI       AvaII       
BbsI        BmyI        BpuAI       BsaJI       BsiHKAI     
BsiYI       BslI        BsmFI       Bsp1286I    BsrI        
BssKI       BstF5I      BstNI       Cac8I       Cfr13I      
DdeI        EcoRII      Fnu4HI      FokI        HaeIII      
HinfI       ItaI        MboII       MnlI        MvaI        
NlaIII      RsaI        Sau96I      ScrFI       SfaNI       
Tsp509I     TspRI       
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