Restriction map for pF1KB6367
 (Circular) (Six-base) MAPSORT of: pF1KB6367.seq  Check: 877  from: 1  to: 3920



pF1KB6367  3920 bp

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:46  ..

AccI GT'mk_AC

Cuts at:    958     958 
   Size:       3920
 
Acc65I G'GTAC_C

Cuts at:    936     936 
   Size:       3920
 
AclI AA'CG_TT

Cuts at:   3760    3760 
   Size:       3920
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    273    1745    2177    2924    3192     273 
   Size:       1472     432     747     268    1001

  Fragments arranged by size: 

                1472    1001     747     432     268
 
AflIII A'CryG_T

Cuts at:   1272    2376    3353    1272 
   Size:       1104     977    1839

  Fragments arranged by size: 

                1839    1104     977
 
AgeI A'CCGG_T

Cuts at:   2308    2308 
   Size:       3920
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:    157     189     157 
   Size:         32    3888
 
AlwNI CAG_nnn'CTG

Cuts at:    222    1225    2792     222 
   Size:       1003    1567    1350

  Fragments arranged by size: 

                1567    1350    1003
 
ApaLI G'TGCA_C

Cuts at:    448    2690     448 
   Size:       2242    1678
 
ApoI r'AATT_y

Cuts at:    787     924    3273    3529     787 
   Size:        137    2349     256    1178

  Fragments arranged by size: 

                2349    1178     256     137
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       3920
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3920
 
AvaI C'yCGr_G

Cuts at:    940     940 
   Size:       3920
 
BamHI G'GATC_C

Cuts at:    945     945 
   Size:       3920
 
BanI G'GyrC_C

Cuts at:    936    1350    1598    1633     936 
   Size:        414     248      35    3223

  Fragments arranged by size: 

                3223     414     248      35
 
BanII G_rGCy'C

Cuts at:    128     934    1964     128 
   Size:        806    1030    2084

  Fragments arranged by size: 

                2084    1030     806
 
BbeI G_GCGC'C

Cuts at:   1354    1602    1354 
   Size:        248    3672
 
BbsI GAAGACnn'nnnn_

Cuts at:    747     843     902     747 
   Size:         96      59    3765

  Fragments arranged by size: 

                3765      96      59
 
BbvCI CC'TCA_GC

Cuts at:    328     328 
   Size:       3920
 
BciVI GTATCCnnnnn_n'

Cuts at:   1818    2579    3920    1818 
   Size:        761    1341    1818

  Fragments arranged by size: 

                1818    1341     761
 
BclI T'GATC_A

Cuts at:   1440    1440 
   Size:       3920
 
BglII A'GATC_T

Cuts at:   1435    3472    1435 
   Size:       2037    1883
 
BlpI GC'TnA_GC

Cuts at:   1032    1032 
   Size:       3920
 
Bme1580I G_kGCm'C

Cuts at:    452    1545    1638    2694     452 
   Size:       1093      93    1056    1678

  Fragments arranged by size: 

                1678    1093    1056      93
 
BmrI ACTGGGnnnn_n'

Cuts at:    795    1132    1213    1548    3692     795 
   Size:        337      81     335    2144    1023

  Fragments arranged by size: 

                2144    1023     337     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2287    2287 
   Size:       3920
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    532     721     532 
   Size:        189    3731
 
Bpu10I CC'TnA_GC

Cuts at:    122     328     376     122 
   Size:        206      48    3666

  Fragments arranged by size: 

                3666     206      48
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    625     815    1096    1975    2467    2765     625 
   Size:        190     281     879     492     298    1780

  Fragments arranged by size: 

                1780     879     492     298     281     190
 
BsaI GGTCTCn'nnnn_

Cuts at:   3624    3624 
   Size:       3920
 
BsaAI yAC'GTr

Cuts at:   1273    1273 
   Size:       3920
 
BsaBI GATnn'nnATC

Cuts at:      6     769    1434    3471       6 
   Size:        763     665    2037     455

  Fragments arranged by size: 

                2037     763     665     455
 
BsaHI Gr'CG_yC

Cuts at:   1351    1599    2301    3797    1351 
   Size:        248     702    1496    1474

  Fragments arranged by size: 

                1496    1474     702     248
 
BsaWI w'CCGG_w

Cuts at:   1109    1630    2308    2582    2729    3140    1109 
   Size:        521     678     274     147     411    1889

  Fragments arranged by size: 

                1889     678     521     411     274     147
 
BseYI C'CCAG_C

Cuts at:   1345    2103    2680    3176    1345 
   Size:        758     577     496    2089

  Fragments arranged by size: 

                2089     758     577     496
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:    462     462 
   Size:       3920
 
BsiEI CG_ry'CG

Cuts at:    104    1462    1508    2716     104 
   Size:       1358      46    1208    1308

  Fragments arranged by size: 

                1358    1308    1208      46
 
BsiHKAI G_wGCw'C

Cuts at:    128     211     382     452     934    1712    1902    2694     128 
   Size:         83     171      70     482     778     190     792    1354

  Fragments arranged by size: 

                1354     792     778     482     190     171      83      70
            
 
Bsp1286I G_dGCh'C

Cuts at:    128     211     382     452     934    1545    1638    1712    1902 
   Size:         83     171      70     482     611      93      74     190

Cuts at:   1902    1964    2694     128 
   Size:         62     730    1354

  Fragments arranged by size: 

                1354     730     611     482     190     171      93      83
                  74      70      62
 
BspEI T'CCGG_A

Cuts at:   3140    3140 
   Size:       3920
 
BspHI T'CATG_A

Cuts at:   3096    3096 
   Size:       3920
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    970    1184    1486    1867     970 
   Size:        214     302     381    3023

  Fragments arranged by size: 

                3023     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50    2212    2266    3750    3915      50 
   Size:       2162      54    1484     165      55

  Fragments arranged by size: 

                2162    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:    165    1832     165 
   Size:       1667    2253
 
BsrFI r'CCGG_y

Cuts at:   1918    2099    2308    1918 
   Size:        181     209    3530

  Fragments arranged by size: 

                3530     209     181
 
BsrGI T'GTAC_A

Cuts at:    594     594 
   Size:       3920
 
BssSI C'ACGA_G

Cuts at:   2191    2549    2191 
   Size:        358    3562
 
BstAPI GCAn_nnn'nTGC

Cuts at:   1297    1297 
   Size:       3920
 
BstBI TT'CG_AA

Cuts at:   2281    2281 
   Size:       3920
 
BstYI r'GATC_y

Cuts at:      1     711     945    1412    1435    1770    2016    3028    3114 
   Size:        710     234     467      23     335     246    1012      86

Cuts at:   3114    3126    3472       1 
   Size:         12     346     449

  Fragments arranged by size: 

                1012     710     467     449     346     335     246     234
                  86      23      12
 
BtgI C'CryG_G

Cuts at:    107     317     107 
   Size:        210    3710
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   1870    2051    2214    1870 
   Size:        181     163    3576

  Fragments arranged by size: 

                3576     181     163
 
BtsI GCAGTG_nn'

Cuts at:   1163    1163 
   Size:       3920
 
DraI TTT'AAA

Cuts at:    919     919 
   Size:       3920
 
DrdI GACnn_nn'nnGTC

Cuts at:   1626    2484    1626 
   Size:        858    3062
 
EaeI y'GGCC_r

Cuts at:    314     873    1505    1679    2070    2097    3442     314 
   Size:        559     632     174     391      27    1345     792

  Fragments arranged by size: 

                1345     792     632     559     391     174      27
 
EagI C'GGCC_G

Cuts at:   1505    1505 
   Size:       3920
 
EarI CTCTTCn'nnn_

Cuts at:   1943    2153    1943 
   Size:        210    3710
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   2128    2438    2584    3730    2128 
   Size:        310     146    1146    2318

  Fragments arranged by size: 

                2318    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:    126     932     126 
   Size:        806    3114
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    273     532     721    1745    2177    2924    3192     273 
   Size:        259     189    1024     432     747     268    1001

  Fragments arranged by size: 

                1024    1001     747     432     268     259     189
 
EcoO109I rG'GnC_Cy

Cuts at:    219    1059     219 
   Size:        840    3080
 
EcoRI G'AATT_C

Cuts at:    924     924 
   Size:       3920
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:    228    1939     228 
   Size:       1711    2209
 
FspI TGC'GCA

Cuts at:   1701    1701 
   Size:       3920
 
HaeII r_GCGC'y

Cuts at:   1354    1602    2624    3603    1354 
   Size:        248    1022     979    1671

  Fragments arranged by size: 

                1671    1022     979     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    504     536     696     728    1762    1792    1794    1824     504 
   Size:         32     160      32    1034      30       2      30    2600

  Fragments arranged by size: 

                2600    1034     160      32      32      30      30       2
            
 
HincII GTy'rAC

Cuts at:    959     959 
   Size:       3920
 
HindIII A'AGCT_T

Cuts at:    504     504 
   Size:       3920
 
KasI G'GCGC_C

Cuts at:   1350    1598    1350 
   Size:        248    3672
 
KpnI G_GTAC'C

Cuts at:    940     940 
   Size:       3920
 
MluI A'CGCG_T

Cuts at:   3353    3353 
   Size:       3920
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:    290    2591    2775     290 
   Size:       2301     184    1435

  Fragments arranged by size: 

                2301    1435     184
 
MscI TGG'CCA

Cuts at:    316    1681     316 
   Size:       1365    2555
 
MslI CAynn'nnrTG

Cuts at:   1909    2036    1909 
   Size:        127    3793
 
MspA1I CmG'CkG

Cuts at:    699    1031    1345    1705    2718    2963     699 
   Size:        332     314     360    1013     245    1656

  Fragments arranged by size: 

                1656    1013     360     332     314     245
 
NaeI GCC'GGC

Cuts at:   2101    2101 
   Size:       3920
 
NarI GG'CG_CC

Cuts at:   1351    1599    1351 
   Size:        248    3672
 
NcoI C'CATG_G

Cuts at:    107     317     107 
   Size:        210    3710
 
NdeI CA'TA_TG

Cuts at:    525     525 
   Size:       3920
 
NgoMIV G'CCGG_C

Cuts at:   2099    2099 
   Size:       3920
 
NspI r_CATG'y

Cuts at:    846     973    1473    2380     846 
   Size:        127     500     907    2386

  Fragments arranged by size: 

                2386     907     500     127
 
PciI A'CATG_T

Cuts at:   2376    2376 
   Size:       3920
 
PflMI CCAn_nnn'nTGG

Cuts at:    222     222 
   Size:       3920
 
PfoI T'CCnGG_A

Cuts at:    215     362     485     215 
   Size:        147     123    3650

  Fragments arranged by size: 

                3650     147     123
 
PmeI GTTT'AAAC

Cuts at:    919     919 
   Size:       3920
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3059    3091    3059 
   Size:         32    3888
 
PpuMI rG'GwC_Cy

Cuts at:    219     219 
   Size:       3920
 
PstI C_TGCA'G

Cuts at:    967    1652     967 
   Size:        685    3235
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       3920
 
PvuII CAG'CTG

Cuts at:    699    1345    1705     699 
   Size:        646     360    2914

  Fragments arranged by size: 

                2914     646     360
 
RsrII CG'GwC_CG

Cuts at:   2115    2115 
   Size:       3920
 
SacI G_AGCT'C

Cuts at:    128     934     128 
   Size:        806    3114
 
SalI G'TCGA_C

Cuts at:    957     957 
   Size:       3920
 
SapI GCTCTTCn'nnn_

Cuts at:   1943    2153    1943 
   Size:        210    3710
 
SbfI CC_TGCA'GG

Cuts at:    967     967 
   Size:       3920
 
ScaI AGT'ACT

Cuts at:   3437    3437 
   Size:       3920
 
SfcI C'TryA_G

Cuts at:     33     276     694     963    1648    2641    2832      33 
   Size:        243     418     269     685     993     191    1121

  Fragments arranged by size: 

                1121     993     685     418     269     243     191
 
SfoI GGC'GCC

Cuts at:   1352    1600    1352 
   Size:        248    3672
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3920
 
SmaI CCC'GGG

Cuts at:    942     942 
   Size:       3920
 
SmlI C'TyrA_G

Cuts at:    640     830    1075    1990    2482    2744     640 
   Size:        190     245     915     492     262    1816

  Fragments arranged by size: 

                1816     915     492     262     245     190
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       3920
 
SphI G_CATG'C

Cuts at:    973    1473     973 
   Size:        500    3420
 
StyI C'CwwG_G

Cuts at:    107     317     632    1054    1408     107 
   Size:        210     315     422     354    2619

  Fragments arranged by size: 

                2619     422     354     315     210
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:   1503    1636    2095    2304    1503 
   Size:        133     459     209    3119

  Fragments arranged by size: 

                3119     459     209     133
 
TatI w'GTAC_w

Cuts at:    594    3435     594 
   Size:       2841    1079
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:    344     372     376     404    3437    3469     344 
   Size:         28       4      28    3033      32     795

  Fragments arranged by size: 

                3033     795      32      28      28       4
 
Tth111I GACn'n_nGTC

Cuts at:   1717    1717 
   Size:       3920
 
XbaI T'CTAG_A

Cuts at:    951     951 
   Size:       3920
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:    138     696    1415     138 
   Size:        558     719    2643

  Fragments arranged by size: 

                2643     719     558
 
XmaI C'CCGG_G

Cuts at:    940     940 
   Size:       3920
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI   AflIII     AgeI     AloI    AlwNI
    ApaLI     ApoI     AseI    AsiSI     AvaI    BamHI     BanI    BanII
     BbeI     BbsI    BbvCI    BciVI     BclI    BglII     BlpI Bme1580I
     BmrI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI
    BsaHI    BsaWI    BseYI     BsgI    BsiEI  BsiHKAI Bsp1286I    BspEI
    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI    BssSI   BstAPI
    BstBI    BstYI     BtgI    BtgZI     BtsI     DraI     DrdI     EaeI
     EagI     EarI     EciI  EcoICRI  Eco57MI EcoO109I    EcoRI     FalI
     FspI    HaeII    Hin4I   HincII  HindIII     KasI     KpnI     MluI
     MmeI     MscI     MslI   MspA1I     NaeI     NarI     NcoI     NdeI
   NgoMIV     NspI     PciI    PflMI     PfoI     PmeI     PpiI    PpuMI
     PstI     PvuI    PvuII    RsrII     SacI     SalI     SapI     SbfI
     ScaI     SfcI     SfoI     SgfI     SmaI     SmlI     SpeI     SphI
     StyI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AfeI    AflII     AhdI     AleI     AlfI     ApaI
     AscI    AvrII     BaeI     BcgI    BfrBI     BglI    BmgBI     BmtI
    BsaXI    BseRI    BsiWI     BsmI    BsmBI   BssHII   BstEII    BstXI
  BstZ17I   Bsu36I     ClaI    CspCI   DraIII    EcoNI    EcoRV     FseI
    FspAI     HpaI     MfeI     NheI     NotI     NruI     NsiI     PacI
     PasI     PmlI    PshAI     PsiI   PspOMI    PspXI     PsrI    SacII
    SanDI    SexAI     SfiI    SgrAI    SnaBI     SrfI     SspI     StuI
     SwaI     XhoI     XmnI     ZraI
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