Restriction map for pF1KB6367
 (Linear) (Six-base) MAPSORT of: KIBB6367.seq  Check: 4664  from: 1  to: 807



KIBB6367.orf

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:46  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     165     807 
   Size:        165     642
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      49      81     807 
   Size:         49      32     726

  Fragments arranged by size: 

                 726      49      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     114     807 
   Size:        114     693
 
ApaLI G'TGCA_C

Cuts at:      0     340     807 
   Size:        340     467
 
ApoI r'AATT_y

Cuts at:      0     679     807 
   Size:        679     128
 
BanII G_rGCy'C

Cuts at:      0      20     807 
   Size:         20     787
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     639     735     794     807 
   Size:        639      96      59      13

  Fragments arranged by size: 

                 639      96      59      13
 
BbvCI CC'TCA_GC

Cuts at:      0     220     807 
   Size:        220     587
 
Bme1580I G_kGCm'C

Cuts at:      0     344     807 
   Size:        344     463
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     687     807 
   Size:        687     120
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     424     613     807 
   Size:        424     189     194

  Fragments arranged by size: 

                 424     194     189
 
Bpu10I CC'TnA_GC

Cuts at:      0      14     220     268     807 
   Size:         14     206      48     539

  Fragments arranged by size: 

                 539     206      48      14
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     517     707     807 
   Size:        517     190     100

  Fragments arranged by size: 

                 517     190     100
 
BsaBI GATnn'nnATC

Cuts at:      0     661     807 
   Size:        661     146
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     354     807 
   Size:        354     453
 
BsiHKAI G_wGCw'C

Cuts at:      0      20     103     274     344     807 
   Size:         20      83     171      70     463

  Fragments arranged by size: 

                 463     171      83      70      20
 
Bsp1286I G_dGCh'C

Cuts at:      0      20     103     274     344     807 
   Size:         20      83     171      70     463

  Fragments arranged by size: 

                 463     171      83      70      20
 
BsrDI GCAATG_nn'

Cuts at:      0      57     807 
   Size:         57     750
 
BsrGI T'GTAC_A

Cuts at:      0     486     807 
   Size:        486     321
 
BstYI r'GATC_y

Cuts at:      0     603     807 
   Size:        603     204
 
BtgI C'CryG_G

Cuts at:      0     209     807 
   Size:        209     598
 
EaeI y'GGCC_r

Cuts at:      0     206     765     807 
   Size:        206     559      42

  Fragments arranged by size: 

                 559     206      42
 
EcoICRI GAG'CTC

Cuts at:      0      18     807 
   Size:         18     789
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     165     424     613     807 
   Size:        165     259     189     194

  Fragments arranged by size: 

                 259     194     189     165
 
EcoO109I rG'GnC_Cy

Cuts at:      0     111     807 
   Size:        111     696
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     120     807 
   Size:        120     687
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     396     428     588     620     807 
   Size:        396      32     160      32     187

  Fragments arranged by size: 

                 396     187     160      32      32
 
HindIII A'AGCT_T

Cuts at:      0     396     807 
   Size:        396     411
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     182     807 
   Size:        182     625
 
MscI TGG'CCA

Cuts at:      0     208     807 
   Size:        208     599
 
MspA1I CmG'CkG

Cuts at:      0     591     807 
   Size:        591     216
 
NcoI C'CATG_G

Cuts at:      0     209     807 
   Size:        209     598
 
NdeI CA'TA_TG

Cuts at:      0     417     807 
   Size:        417     390
 
NspI r_CATG'y

Cuts at:      0     738     807 
   Size:        738      69
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     114     807 
   Size:        114     693
 
PfoI T'CCnGG_A

Cuts at:      0     107     254     377     807 
   Size:        107     147     123     430

  Fragments arranged by size: 

                 430     147     123     107
 
PpuMI rG'GwC_Cy

Cuts at:      0     111     807 
   Size:        111     696
 
PvuII CAG'CTG

Cuts at:      0     591     807 
   Size:        591     216
 
SacI G_AGCT'C

Cuts at:      0      20     807 
   Size:         20     787
 
SfcI C'TryA_G

Cuts at:      0     168     586     807 
   Size:        168     418     221

  Fragments arranged by size: 

                 418     221     168
 
SmlI C'TyrA_G

Cuts at:      0     532     722     807 
   Size:        532     190      85

  Fragments arranged by size: 

                 532     190      85
 
StyI C'CwwG_G

Cuts at:      0     209     524     807 
   Size:        209     315     283

  Fragments arranged by size: 

                 315     283     209
 
TatI w'GTAC_w

Cuts at:      0     486     807 
   Size:        486     321
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     236     264     268     296     807 
   Size:        236      28       4      28     511

  Fragments arranged by size: 

                 511     236      28      28       4
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0      30     588     807 
   Size:         30     558     219

  Fragments arranged by size: 

                 558     219      30
 

 Enzymes that do cut:

     AcuI     AloI    AlwNI    ApaLI     ApoI    BanII     BbsI    BbvCI
 Bme1580I     BmrI     BpmI   Bpu10I    BpuEI    BsaBI     BsgI  BsiHKAI
 Bsp1286I    BsrDI    BsrGI    BstYI     BtgI     EaeI  EcoICRI  Eco57MI
 EcoO109I     FalI    Hin4I  HindIII     MmeI     MscI   MspA1I     NcoI
     NdeI     NspI    PflMI     PfoI    PpuMI    PvuII     SacI     SfcI
     SmlI     StyI     TatI     TstI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     ApaI     AscI     AseI    AsiSI
     AvaI    AvrII     BaeI    BamHI     BanI     BbeI     BcgI    BciVI
     BclI    BfrBI     BglI    BglII     BlpI    BmgBI     BmtI     BplI
     BsaI    BsaAI    BsaHI    BsaWI    BsaXI    BseRI    BseYI    BsiEI
    BsiWI     BsmI    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrFI
   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I
    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EagI
     EarI     EciI    EcoNI    EcoRI    EcoRV     FseI     FspI    FspAI
    HaeII   HincII     HpaI     KasI     KpnI     MfeI     MluI     MslI
     NaeI     NarI   NgoMIV     NheI     NotI     NruI     NsiI     PacI
     PasI     PciI     PmeI     PmlI     PpiI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PstI     PvuI    RsrII    SacII     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI
     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI
    TaqII  Tth111I     XbaI     XhoI     XmaI     XmnI     ZraI
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