Restriction map for pF1KB6377
##################################
# NO CUT ENZYMES (  178 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AciI        AclI        AflII       AgeI        AhdI        
Alw44I      AocI        Aor51HI     ApaI        ApaLI       
AscI        AseI        AsnI        Asp718I     AspEI       
AspHI       AspI        AvaI        AvaII       AviII       
BamHI       BanI        BanII       BbeI        BbrPI       
BbvCI       BcgI        BciVI       BcnI        BfrI        
BglI        BglII       BlpI        BmyI        Bpu1102I    
BsaAI       BsaBI       BsaWI       BseAI       BsgI        
BsiEI       BsiHKAI     BsiWI       BsiYI       BslI        
BsmFI       BsmI        BsoBI       Bsp1286I    Bsp1407I    
BspDI       BspEI       BspHI       BspMI       BsrBI       
BsrFI       BsrGI       BssHII      BssSI       Bst1107I    
BstAPI      BstBI       BstEII      BstPI       BstUI       
BstXI       BstYI       BstZ17I     Bsu36I      Cac8I       
CelII       CfoI        Cfr10I      ClaI        CpoI        
DraI        DraII       DraIII      DrdI        DsaI        
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
Eco81I      EcoNI       EcoO109I    EcoO65I     EcoRV       
EcoT22I     FauI        FseI        FspI        HaeII       
HapII       HhaI        HinP1I      HincII      HindII      
HpaI        HpaII       HphI        KasI        KpnI        
KspI        MaeII       MamI        MflI        MluI        
MroI        MslI        MspA1I      MspI        MvnI        
NaeI        NarI        NciI        NcoI        NdeI        
NgoMIV      NheI        NotI        NruI        NsiI        
NspV        PacI        PaeR7I      PflFI       PflMI       
PinAI       PmaCI       PmeI        PmlI        PpuMI       
PshAI       PshBI       Psp1406I    PstI        PvuI        
PvuII       RcaI        RsrII       SacI        SacII       
SalI        SapI        SbfI        ScaI        SexAI       
SfcI        SfiI        SfoI        SfuI        SgrAI       
SmaI        SmlI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       SspI        StuI        SwaI        
Tth111I     Van91I      XbaI        XcmI        XhoI        
XhoII       XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   38 enzymes) #
###################################

AcyI          412  GACGCC               G[GA]^CG[CT]C
AfaI          450  GTAC                 GT^AC
AflIII         17  ACATGT               A^C[GA][CT]GT
AlwNI         793  CAGATACTG            CAG[ACGT][ACGT][ACGT]^CTG
AvrII         331  CCTAGG               C^CTAGG
BalI            2  TGGCCA               TGG^CCA
BfaI          332  CTAG                 C^TAG
BlnI          331  CCTAGG               C^CTAGG
BpmI          289  CTGGAG               CTGGAG(16/14)
BsaHI         412  GACGCC               G[GA]^CG[CT]C
BsaI          652  GAGACC               GGTCTC(1/5)
BsaJI         331  CCTAGG               C^C[ACGT][ACGT]GG
BseRI         661  CTCCTC               GAGGAG(10/8)
BsmBI         249  GAGACG               CGTCTC(1/5)
BspLU11I       17  ACATGT               A^CATGT
BsrDI         359  GCAATG               GCAATG(2/0)
EaeI            2  TGGCCA               [CT]^GGCC[GA]
EcoRI         397  GAATTC               G^AATTC
EcoT14I       331  CCTAGG               C^C[AT][AT]GG
HgaI          412  GACGC                GACGC(5/10)
Hin1I         412  GACGCC               G[GA]^CG[CT]C
HindIII       190  AAGCTT               A^AGCTT
MaeI          332  CTAG                 C^TAG
MfeI          568  CAATTG               C^AATTG
MluNI           2  TGGCCA               TGG^CCA
MscI            2  TGGCCA               TGG^CCA
MseI          262  TTAA                 T^TAA
MunI          568  CAATTG               C^AATTG
MwoI          350  GCTTCCTGAGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NlaIV         768  GGGGCC               GG[ACGT]^[ACGT]CC
NspI           17  ACATGT               [GA]CATG^[CT]
PleI          307  GACTC                GAGTC(4/5)
RsaI          450  GTAC                 GT^AC
StyI          331  CCTAGG               C^C[AT][AT]GG
TaqI          435  TCGA                 T^CGA
Tru9I         262  TTAA                 T^TAA
Tsp45I        577  GTGAC                ^GT[GC]AC
TthHB8I       435  TCGA                 T^CGA


###################################
# TWO CUT ENZYMES (   18 enzymes) #
###################################

AcsI          397  GAATTC               [GA]^AATT[CT]
              493  AAATTT              
AlwI          144  GGATC                GGATC(4/5)
              383  GGATC               
ApoI          397  GAATTC               [GA]^AATT[CT]
              493  AAATTT              
Asp700I        96  GAAATCCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              693  GAACTATTTC          
BclI           75  TGATCA               T^GATCA
              444  TGATCA              
BssKI         288  CCTGG                ^CC[ACGT]GG
              807  CCAGG               
BstNI         288  CCTGG                CC^[AT]GG
              807  CCAGG               
Cfr13I        121  GGCCC                G^G[ACGT]CC
              769  GGGCC               
EarI          664  CTCTTC               CTCTTC(1/4)
              246  GAAGAG              
EcoRII        288  CCTGG                ^CC[AT]GG
              807  CCAGG               
FbaI           75  TGATCA               T^GATCA
              444  TGATCA              
Ksp632I       664  CTCTTC               CTCTTC(1/4)
              246  GAAGAG              
MaeIII         41  GTTAC                ^GT[ACGT]AC
              577  GTGAC               
MvaI          288  CCTGG                CC^[AT]GG
              807  CCAGG               
Sau96I        121  GGCCC                G^G[ACGT]CC
              769  GGGCC               
ScrFI         288  CCTGG                CC^[ACGT]GG
              807  CCAGG               
SfaNI         529  GATGC                GCATC(5/9)
              618  GATGC               
XmnI           96  GAAATCCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              693  GAACTATTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   24 enzymes) #
#########################################

AluI        BbsI        BbvI        BpuAI       BsmAI       
BsrI        BstF5I      DdeI        DpnII       Fnu4HI      
FokI        HaeIII      HinfI       ItaI        MboI        
MboII       MnlI        NdeII       NlaIII      Sau3AI      
TfiI        TseI        Tsp509I     TspRI       
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