Restriction map for pF1KB6377
 (Circular) (Six-base) MAPSORT of: pF1KB6377.seq  Check: 6993  from: 1  to: 3926



pF1KB6377  3926 bp

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:46  ..

AccI GT'mk_AC

Cuts at:    964     964 
   Size:       3926
 
Acc65I G'GTAC_C

Cuts at:    942     942 
   Size:       3926
 
AclI AA'CG_TT

Cuts at:   3766    3766 
   Size:       3926
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    160     370     739    1751    2183    2930    3198     160 
   Size:        210     369    1012     432     747     268     888

  Fragments arranged by size: 

                1012     888     747     432     369     268     210
 
AflIII A'CryG_T

Cuts at:    125    1278    2382    3359     125 
   Size:       1153    1104     977     692

  Fragments arranged by size: 

                1153    1104     977     692
 
AgeI A'CCGG_T

Cuts at:   2314    2314 
   Size:       3926
 
AlwNI CAG_nnn'CTG

Cuts at:    906    1231    2798     906 
   Size:        325    1567    2034

  Fragments arranged by size: 

                2034    1567     325
 
ApaLI G'TGCA_C

Cuts at:   2696    2696 
   Size:       3926
 
ApoI r'AATT_y

Cuts at:    505     601     930    3279    3535     505 
   Size:         96     329    2349     256     896

  Fragments arranged by size: 

                2349     896     329     256      96
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       3926
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3926
 
AvaI C'yCGr_G

Cuts at:    946     946 
   Size:       3926
 
AvrII C'CTAG_G

Cuts at:    439     439 
   Size:       3926
 
BamHI G'GATC_C

Cuts at:    951     951 
   Size:       3926
 
BanI G'GyrC_C

Cuts at:    942    1356    1604    1639     942 
   Size:        414     248      35    3229

  Fragments arranged by size: 

                3229     414     248      35
 
BanII G_rGCy'C

Cuts at:    940    1970     940 
   Size:       1030    2896
 
BbeI G_GCGC'C

Cuts at:   1360    1608    1360 
   Size:        248    3678
 
BbsI GAAGACnn'nnnn_

Cuts at:    140     173     707     140 
   Size:         33     534    3359

  Fragments arranged by size: 

                3359     534      33
 
BciVI GTATCCnnnnn_n'

Cuts at:   1824    2585    3926    1824 
   Size:        761    1341    1824

  Fragments arranged by size: 

                1824    1341     761
 
BclI T'GATC_A

Cuts at:    183     552    1446     183 
   Size:        369     894    2663

  Fragments arranged by size: 

                2663     894     369
 
BglII A'GATC_T

Cuts at:   1441    3478    1441 
   Size:       2037    1889
 
BlpI GC'TnA_GC

Cuts at:   1038    1038 
   Size:       3926
 
Bme1580I G_kGCm'C

Cuts at:   1551    1644    2700    1551 
   Size:         93    1056    2777

  Fragments arranged by size: 

                2777    1056      93
 
BmrI ACTGGGnnnn_n'

Cuts at:    867    1138    1219    1554    3698     867 
   Size:        271      81     335    2144    1095

  Fragments arranged by size: 

                2144    1095     335     271      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2293    2293 
   Size:       3926
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    418     418 
   Size:       3926
 
Bpu10I CC'TnA_GC

Cuts at:    463     463 
   Size:       3926
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1102    1981    2473    2771    1102 
   Size:        879     492     298    2257

  Fragments arranged by size: 

                2257     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    754    3630     754 
   Size:       2876    1050
 
BsaAI yAC'GTr

Cuts at:   1279    1279 
   Size:       3926
 
BsaBI GATnn'nnATC

Cuts at:      6    1440    3477       6 
   Size:       1434    2037     455

  Fragments arranged by size: 

                2037    1434     455
 
BsaHI Gr'CG_yC

Cuts at:    521    1357    1605    2307    3803     521 
   Size:        836     248     702    1496     644

  Fragments arranged by size: 

                1496     836     702     644     248
 
BsaWI w'CCGG_w

Cuts at:   1115    1636    2314    2588    2735    3146    1115 
   Size:        521     678     274     147     411    1895

  Fragments arranged by size: 

                1895     678     521     411     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:    160     190     160 
   Size:         30    3896
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    760     760 
   Size:       3926
 
BseYI C'CCAG_C

Cuts at:   1351    2109    2686    3182    1351 
   Size:        758     577     496    2095

  Fragments arranged by size: 

                2095     758     577     496
 
BsiEI CG_ry'CG

Cuts at:    104    1468    1514    2722     104 
   Size:       1364      46    1208    1308

  Fragments arranged by size: 

                1364    1308    1208      46
 
BsiHKAI G_wGCw'C

Cuts at:    940    1718    1908    2700     940 
   Size:        778     190     792    2166

  Fragments arranged by size: 

                2166     792     778     190
 
BsmBI CGTCTCn'nnnn_

Cuts at:    351     351 
   Size:       3926
 
Bsp1286I G_dGCh'C

Cuts at:    940    1551    1644    1718    1908    1970    2700     940 
   Size:        611      93      74     190      62     730    2166

  Fragments arranged by size: 

                2166     730     611     190      93      74      62
 
BspEI T'CCGG_A

Cuts at:   3146    3146 
   Size:       3926
 
BspHI T'CATG_A

Cuts at:   3102    3102 
   Size:       3926
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    976    1190    1492    1873     976 
   Size:        214     302     381    3029

  Fragments arranged by size: 

                3029     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50    2218    2272    3756    3921      50 
   Size:       2168      54    1484     165      55

  Fragments arranged by size: 

                2168    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:    474    1838     474 
   Size:       1364    2562
 
BsrFI r'CCGG_y

Cuts at:   1924    2105    2314    1924 
   Size:        181     209    3536

  Fragments arranged by size: 

                3536     209     181
 
BssSI C'ACGA_G

Cuts at:   2197    2555    2197 
   Size:        358    3568
 
BstAPI GCAn_nnn'nTGC

Cuts at:   1303    1303 
   Size:       3926
 
BstBI TT'CG_AA

Cuts at:   2287    2287 
   Size:       3926
 
BstYI r'GATC_y

Cuts at:      1     951    1418    1441    1776    2022    3034    3120    3132 
   Size:        950     467      23     335     246    1012      86      12

Cuts at:   3132    3478       1 
   Size:        346     449

  Fragments arranged by size: 

                1012     950     467     449     346     335     246      86
                  23      12
 
BtgI C'CryG_G

Cuts at:    107     107 
   Size:       3926
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    390    1876    2057    2220     390 
   Size:       1486     181     163    2096

  Fragments arranged by size: 

                2096    1486     181     163
 
BtsI GCAGTG_nn'

Cuts at:    602    1169     602 
   Size:        567    3359
 
DraI TTT'AAA

Cuts at:    925     925 
   Size:       3926
 
DrdI GACnn_nn'nnGTC

Cuts at:   1632    2490    1632 
   Size:        858    3068
 
EaeI y'GGCC_r

Cuts at:    110    1511    1685    2076    2103    3448     110 
   Size:       1401     174     391      27    1345     588

  Fragments arranged by size: 

                1401    1345     588     391     174      27
 
EagI C'GGCC_G

Cuts at:   1511    1511 
   Size:       3926
 
EarI CTCTTCn'nnn_

Cuts at:    349     778    1949    2159     349 
   Size:        429    1171     210    2116

  Fragments arranged by size: 

                2116    1171     429     210
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   2134    2444    2590    3736    2134 
   Size:        310     146    1146    2324

  Fragments arranged by size: 

                2324    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:    938     938 
   Size:       3926
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    160     370     418     739    1751    2183    2930    3198     160 
   Size:        210      48     321    1012     432     747     268     888

  Fragments arranged by size: 

                1012     888     747     432     321     268     210      48
            
 
EcoO109I rG'GnC_Cy

Cuts at:   1065    1065 
   Size:       3926
 
EcoRI G'AATT_C

Cuts at:    505     930     505 
   Size:        425    3501
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:    321    1945     321 
   Size:       1624    2302
 
FspI TGC'GCA

Cuts at:   1707    1707 
   Size:       3926
 
HaeII r_GCGC'y

Cuts at:   1360    1608    2630    3609    1360 
   Size:        248    1022     979    1677

  Fragments arranged by size: 

                1677    1022     979     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    160     175     192     207    1768    1798    1800    1830     160 
   Size:         15      17      15    1561      30       2      30    2256

  Fragments arranged by size: 

                2256    1561      30      30      17      15      15       2
            
 
HincII GTy'rAC

Cuts at:    965     965 
   Size:       3926
 
HindIII A'AGCT_T

Cuts at:    298     298 
   Size:       3926
 
KasI G'GCGC_C

Cuts at:   1356    1604    1356 
   Size:        248    3678
 
KpnI G_GTAC'C

Cuts at:    946     946 
   Size:       3926
 
MfeI C'AATT_G

Cuts at:    676     676 
   Size:       3926
 
MluI A'CGCG_T

Cuts at:   3359    3359 
   Size:       3926
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   2597    2781    2597 
   Size:        184    3742
 
MscI TGG'CCA

Cuts at:    112    1687     112 
   Size:       1575    2351
 
MslI CAynn'nnrTG

Cuts at:   1915    2042    1915 
   Size:        127    3799
 
MspA1I CmG'CkG

Cuts at:   1037    1351    1711    2724    2969    1037 
   Size:        314     360    1013     245    1994

  Fragments arranged by size: 

                1994    1013     360     314     245
 
NaeI GCC'GGC

Cuts at:   2107    2107 
   Size:       3926
 
NarI GG'CG_CC

Cuts at:   1357    1605    1357 
   Size:        248    3678
 
NcoI C'CATG_G

Cuts at:    107     107 
   Size:       3926
 
NgoMIV G'CCGG_C

Cuts at:   2105    2105 
   Size:       3926
 
NspI r_CATG'y

Cuts at:    129     979    1479    2386     129 
   Size:        850     500     907    1669

  Fragments arranged by size: 

                1669     907     850     500
 
PciI A'CATG_T

Cuts at:    125    2382     125 
   Size:       2257    1669
 
PmeI GTTT'AAAC

Cuts at:    925     925 
   Size:       3926
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3065    3097    3065 
   Size:         32    3894
 
PsiI TTA'TAA

Cuts at:    620     620 
   Size:       3926
 
PstI C_TGCA'G

Cuts at:    973    1658     973 
   Size:        685    3241
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       3926
 
PvuII CAG'CTG

Cuts at:   1351    1711    1351 
   Size:        360    3566
 
RsrII CG'GwC_CG

Cuts at:   2121    2121 
   Size:       3926
 
SacI G_AGCT'C

Cuts at:    940     940 
   Size:       3926
 
SalI G'TCGA_C

Cuts at:    963     963 
   Size:       3926
 
SapI GCTCTTCn'nnn_

Cuts at:   1949    2159    1949 
   Size:        210    3716
 
SbfI CC_TGCA'GG

Cuts at:    973     973 
   Size:       3926
 
ScaI AGT'ACT

Cuts at:   3443    3443 
   Size:       3926
 
SfcI C'TryA_G

Cuts at:     33     969    1654    2647    2838      33 
   Size:        936     685     993     191    1121

  Fragments arranged by size: 

                1121     993     936     685     191
 
SfoI GGC'GCC

Cuts at:   1358    1606    1358 
   Size:        248    3678
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3926
 
SmaI CCC'GGG

Cuts at:    948     948 
   Size:       3926
 
SmlI C'TyrA_G

Cuts at:   1081    1996    2488    2750    1081 
   Size:        915     492     262    2257

  Fragments arranged by size: 

                2257     915     492     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       3926
 
SphI G_CATG'C

Cuts at:    979    1479     979 
   Size:        500    3426
 
StyI C'CwwG_G

Cuts at:    107     439    1060    1414     107 
   Size:        332     621     354    2619

  Fragments arranged by size: 

                2619     621     354     332
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    601     849    1509    1642    2101    2310     601 
   Size:        248     660     133     459     209    2217

  Fragments arranged by size: 

                2217     660     459     248     209     133
 
TatI w'GTAC_w

Cuts at:   3441    3441 
   Size:       3926
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   3443    3475    3443 
   Size:         32    3894
 
Tth111I GACn'n_nGTC

Cuts at:   1723    1723 
   Size:       3926
 
XbaI T'CTAG_A

Cuts at:    957     957 
   Size:       3926
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   1421    1421 
   Size:       3926
 
XmaI C'CCGG_G

Cuts at:    946     946 
   Size:       3926
 
XmnI GAAnn'nnTTC

Cuts at:    208     805     208 
   Size:        597    3329
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI   AflIII     AgeI    AlwNI    ApaLI
     ApoI     AseI    AsiSI     AvaI    AvrII    BamHI     BanI    BanII
     BbeI     BbsI    BciVI     BclI    BglII     BlpI Bme1580I     BmrI
     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI    BsaXI    BseRI    BseYI    BsiEI  BsiHKAI    BsmBI Bsp1286I
    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BssSI   BstAPI
    BstBI    BstYI     BtgI    BtgZI     BtsI     DraI     DrdI     EaeI
     EagI     EarI     EciI  EcoICRI  Eco57MI EcoO109I    EcoRI     FalI
     FspI    HaeII    Hin4I   HincII  HindIII     KasI     KpnI     MfeI
     MluI     MmeI     MscI     MslI   MspA1I     NaeI     NarI     NcoI
   NgoMIV     NspI     PciI     PmeI     PpiI     PsiI     PstI     PvuI
    PvuII    RsrII     SacI     SalI     SapI     SbfI     ScaI     SfcI
     SfoI     SgfI     SmaI     SmlI     SpeI     SphI     StyI    TaqII
     TatI     TstI  Tth111I     XbaI     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AfeI    AflII     AhdI     AleI     AlfI     AloI
     ApaI     AscI     BaeI    BbvCI     BcgI    BfrBI     BglI    BmgBI
     BmtI     BsgI    BsiWI     BsmI    BsrGI   BssHII   BstEII    BstXI
  BstZ17I   Bsu36I     ClaI    CspCI   DraIII    EcoNI    EcoRV     FseI
    FspAI     HpaI     NdeI     NheI     NotI     NruI     NsiI     PacI
     PasI    PflMI     PfoI     PmlI    PpuMI    PshAI   PspOMI    PspXI
     PsrI    SacII    SanDI    SexAI     SfiI    SgrAI    SnaBI     SrfI
     SspI     StuI     SwaI     XhoI     ZraI
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