Restriction map for pF1KB6480
##################################
# NO CUT ENZYMES (  141 enzymes) #
##################################

Acc65I      AclI        AcsI        AflII       AflIII      
AgeI        Alw44I      AlwI        AocI        Aor51HI     
ApaI        ApaLI       ApoI        AscI        AseI        
AsnI        Asp718I     AspI        AvaI        AviII       
AvrII       BalI        BamHI       BanI        BanII       
BbeI        BbrPI       BcgI        BciVI       BclI        
BcnI        BfaI        BfrI        BglII       BlnI        
BsaAI       BsaBI       BsaI        BsiWI       BsmAI       
BsmBI       BsmI        BsoBI       BspDI       BspHI       
BspLU11I    BsrDI       BsrFI       BssHII      Bst1107I    
BstAPI      BstBI       BstYI       BstZ17I     Bsu36I      
CfoI        Cfr10I      ClaI        CpoI        DraIII      
DrdI        Eco47III    Eco81I      EcoNI       EcoRI       
EcoRV       EcoT22I     FbaI        FseI        FspI        
HaeII       HhaI        HinP1I      HincII      HindII      
HindIII     HpaI        KasI        KpnI        MaeI        
MamI        MfeI        MflI        MluI        MluNI       
MscI        MunI        NaeI        NarI        NciI        
NcoI        NdeI        NgoMIV      NheI        NotI        
NruI        NsiI        NspI        NspV        PacI        
PaeR7I      PflFI       PflMI       PinAI       PmaCI       
PmeI        PmlI        PshAI       PshBI       Psp1406I    
PstI        PvuI        RcaI        RsrII       SacI        
SalI        SapI        SbfI        ScaI        SexAI       
SfcI        SfiI        SfoI        SfuI        SgrAI       
SmaI        SmlI        SnaBI       SpeI        SphI        
Sse8387I    SspI        SwaI        Tth111I     Van91I      
XbaI        XcmI        XhoI        XhoII       XmaCI       
XmaI        


###################################
# ONE CUT ENZYMES (   51 enzymes) #
###################################

AatII         943  GACGTC               GACGT^C
AccI          184  GTCTAC               GT^[AC][GT]AC
AccII          60  CGCG                 CG^CG
AccIII        975  TCCGGA               T^CCGGA
AcyI          943  GACGTC               G[GA]^CG[CT]C
AlwNI         133  CAGGTCCTG            CAG[ACGT][ACGT][ACGT]^CTG
AspHI         359  GAGCAC               G[AT]GC[AT]^C
BbsI           43  GTCTTC               GAAGAC(2/6)
BglI           55  GCCGCCGCGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI          349  GCTGAGC              GC^T[ACGT]AGC
BmyI          359  GAGCAC               G[AGT]GC[ACT]^C
Bpu1102I      349  GCTGAGC              GC^T[ACGT]AGC
BpuAI          43  GTCTTC               GAAGAC(2/6)
BsaHI         943  GACGTC               G[GA]^CG[CT]C
BsaWI         975  TCCGGA               [AT]^CCGG[AT]
BseAI         975  TCCGGA               T^CCGGA
BsgI          193  GTGCAG               GTGCAG(16/14)
BsiHKAI       359  GAGCAC               G[AT]GC[AT]^C
Bsp1286I      359  GAGCAC               G[AGT]GC[ACT]^C
BspEI         975  TCCGGA               T^CCGGA
BspMI         132  GCAGGT               ACCTGC(4/8)
BsrBI          92  CCGCTC               CCGCTC(-3/-3)
BssSI         171  CACGAG               CACGAG(-5/-1)
BstEII        441  GGTGACC              G^GT[ACGT]ACC
BstPI         441  GGTGACC              G^GT[ACGT]ACC
BstUI          60  CGCG                 CG^CG
BstXI         587  CCATTTCATTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII         349  GCTGAGC              GC^T[ACGT]AGC
EaeI          227  CGGCCG               [CT]^GGCC[GA]
EagI          227  CGGCCG               C^GGCCG
EarI          874  GAAGAG               CTCTTC(1/4)
EclXI         227  CGGCCG               C^GGCCG
Eco52I        227  CGGCCG               C^GGCCG
EcoO65I       441  GGTGACC              G^GT[ACGT]ACC
HapII         976  CCGG                 C^CGG
HgaI            9  GACGC                GACGC(5/10)
Hin1I         943  GACGTC               G[GA]^CG[CT]C
HpaII         976  CCGG                 C^CGG
Ksp632I       874  GAAGAG               CTCTTC(1/4)
KspI           59  CCGCGG               CCGC^GG
MaeII         944  ACGT                 A^CGT
MroI          975  TCCGGA               T^CCGGA
MspI          976  CCGG                 C^CGG
MvnI           60  CGCG                 CG^CG
NlaIII        237  CATG                 CATG^
PpuMI         134  AGGTCCT              [GA]G^G[AT]CC[CT]
PvuII          85  CAGCTG               CAG^CTG
SacII          59  CCGCGG               CCGC^GG
TaqI          345  TCGA                 T^CGA
Tsp509I       253  AATT                 ^AATT
TthHB8I       345  TCGA                 T^CGA


###################################
# TWO CUT ENZYMES (   28 enzymes) #
###################################

AhdI          232  GACATCATGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
              952  GACTCTGTGTC         
Asp700I       761  GAACATTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              991  GAAGATGTTC          
AspEI         232  GACATCATGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
              952  GACTCTGTGTC         
AvaII         135  GGTCC                G^G[AT]CC
              807  GGTCC               
BbvCI          97  CCTCAGC              CCTCAGC(-5/-2)
              302  CCTCAGC             
BpmI          425  CTCCAG               CTGGAG(16/14)
              612  CTCCAG              
BseRI          95  CTCCTC               GAGGAG(10/8)
              477  CTCCTC              
BsiEI           4  CGACCG               CG[GA][CT]^CG
              227  CGGCCG              
BsmFI         950  GGGAC                GGGAC(10/14)
              299  GTCCC               
Bsp1407I      119  TGTACA               T^GTACA
              256  TGTACA              
BsrGI         119  TGTACA               T^GTACA
              256  TGTACA              
DpnII         264  GATC                 ^GATC
              750  GATC                
DraI          199  TTTAAA               TTT^AAA
              882  TTTAAA              
DraII         134  AGGTCCT              [GA]G^G[ACGT]CC[CT]
              567  AGGCCCT             
Eam1105I      232  GACATCATGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
              952  GACTCTGTGTC         
EcoO109I      134  AGGTCCT              [GA]G^G[ACGT]CC[CT]
              567  AGGCCCT             
EcoT14I       388  CCTTGG               C^C[AT][AT]GG
              914  CCTTGG              
FauI           66  CCCGC                CCCGC(4/6)
               91  CCCGC               
MboI          264  GATC                 ^GATC
              750  GATC                
MslI          243  CATAGGGGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              435  CATTGAGGTG          
MspA1I         59  CCGCGG               C[AC]G^C[GT]G
               85  CAGCTG              
NdeII         264  GATC                 ^GATC
              750  GATC                
Sau3AI        264  GATC                 ^GATC
              750  GATC                
SfaNI         718  GATGC                GCATC(5/9)
              857  GCATC               
SspBI         119  TGTACA               T^GTACA
              256  TGTACA              
StyI          388  CCTTGG               C^C[AT][AT]GG
              914  CCTTGG              
TfiI          111  GATTC                G^A[AT]TC
              551  GAATC               
XmnI          761  GAACATTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              991  GAAGATGTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   38 enzymes) #
#########################################

AciI        AfaI        AluI        BbvI        BsaJI       
BsiYI       BslI        BsrI        BssKI       BstF5I      
BstNI       Cac8I       Cfr13I      DdeI        DsaI        
EcoRII      Fnu4HI      FokI        HaeIII      HinfI       
HphI        ItaI        MaeIII      MboII       MnlI        
MseI        MvaI        MwoI        NlaIV       PleI        
RsaI        Sau96I      ScrFI       StuI        Tru9I       
TseI        Tsp45I      TspRI       
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