Restriction map for pF1KB6480
 (Linear) (Six-base) MAPSORT of: KIBB6480.seq  Check: 121  from: 1  to: 1011



KIBB6480.orf

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:47  ..

AatII G_ACGT'C

Cuts at:      0     947    1011 
   Size:        947      64
 
AccI GT'mk_AC

Cuts at:      0     185    1011 
   Size:        185     826
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     718    1011 
   Size:        718     293
 
AhdI GACnn_n'nnGTC

Cuts at:      0     237     957    1011 
   Size:        237     720      54

  Fragments arranged by size: 

                 720     237      54
 
AlwNI CAG_nnn'CTG

Cuts at:      0     138    1011 
   Size:        138     873
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      36    1011 
   Size:         36     975
 
BbvCI CC'TCA_GC

Cuts at:      0      98     303    1011 
   Size:         98     205     708

  Fragments arranged by size: 

                 708     205      98
 
BglI GCCn_nnn'nGGC

Cuts at:      0      61    1011 
   Size:         61     950
 
BlpI GC'TnA_GC

Cuts at:      0     350    1011 
   Size:        350     661
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     546    1011 
   Size:        546     465
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     410     597    1011 
   Size:        410     187     414

  Fragments arranged by size: 

                 414     410     187
 
Bpu10I CC'TnA_GC

Cuts at:      0      98     160     303    1011 
   Size:         98      62     143     708

  Fragments arranged by size: 

                 708     143      98      62
 
BsaHI Gr'CG_yC

Cuts at:      0     944    1011 
   Size:        944      67
 
BsaWI w'CCGG_w

Cuts at:      0     975    1011 
   Size:        975      36
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      86     468    1011 
   Size:         86     382     543

  Fragments arranged by size: 

                 543     382      86
 
BseYI C'CCAG_C

Cuts at:      0      83     354    1011 
   Size:         83     271     657

  Fragments arranged by size: 

                 657     271      83
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     214    1011 
   Size:        214     797
 
BsiEI CG_ry'CG

Cuts at:      0       7     230    1011 
   Size:          7     223     781

  Fragments arranged by size: 

                 781     223       7
 
BsiHKAI G_wGCw'C

Cuts at:      0     363    1011 
   Size:        363     648
 
Bsp1286I G_dGCh'C

Cuts at:      0     363    1011 
   Size:        363     648
 
BspEI T'CCGG_A

Cuts at:      0     975    1011 
   Size:        975      36
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     123    1011 
   Size:        123     888
 
BsrBI CCG'CTC

Cuts at:      0      94    1011 
   Size:         94     917
 
BsrGI T'GTAC_A

Cuts at:      0     119     256    1011 
   Size:        119     137     755

  Fragments arranged by size: 

                 755     137     119
 
BssSI C'ACGA_G

Cuts at:      0     171    1011 
   Size:        171     840
 
BstEII G'GTnAC_C

Cuts at:      0     441    1011 
   Size:        441     570
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     594    1011 
   Size:        594     417
 
BtgI C'CryG_G

Cuts at:      0      59     506     755    1011 
   Size:         59     447     249     256

  Fragments arranged by size: 

                 447     256     249      59
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     477    1011 
   Size:        477     534
 
BtsI GCAGTG_nn'

Cuts at:      0     287    1011 
   Size:        287     724
 
DraI TTT'AAA

Cuts at:      0     201     884    1011 
   Size:        201     683     127

  Fragments arranged by size: 

                 683     201     127
 
EaeI y'GGCC_r

Cuts at:      0     227    1011 
   Size:        227     784
 
EagI C'GGCC_G

Cuts at:      0     227    1011 
   Size:        227     784
 
EarI CTCTTCn'nnn_

Cuts at:      0     869    1011 
   Size:        869     142
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     410     597     718    1011 
   Size:        410     187     121     293

  Fragments arranged by size: 

                 410     293     187     121
 
EcoO109I rG'GnC_Cy

Cuts at:      0     135     568    1011 
   Size:        135     433     443

  Fragments arranged by size: 

                 443     433     135
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     223     255     943     975    1011 
   Size:        223      32     688      32      36

  Fragments arranged by size: 

                 688     223      36      32      32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     579    1011 
   Size:        579     432
 
MslI CAynn'nnrTG

Cuts at:      0     247     439    1011 
   Size:        247     192     572

  Fragments arranged by size: 

                 572     247     192
 
MspA1I CmG'CkG

Cuts at:      0      61      87    1011 
   Size:         61      26     924

  Fragments arranged by size: 

                 924      61      26
 
PpuMI rG'GwC_Cy

Cuts at:      0     135    1011 
   Size:        135     876
 
PvuII CAG'CTG

Cuts at:      0      87    1011 
   Size:         87     924
 
SacII CC_GC'GG

Cuts at:      0      62    1011 
   Size:         62     949
 
StuI AGG'CCT

Cuts at:      0     177     824     913    1011 
   Size:        177     647      89      98

  Fragments arranged by size: 

                 647     177      98      89
 
StyI C'CwwG_G

Cuts at:      0     388     914    1011 
   Size:        388     526      97

  Fragments arranged by size: 

                 526     388      97
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      21     366    1011 
   Size:         21     345     645

  Fragments arranged by size: 

                 645     345      21
 
TatI w'GTAC_w

Cuts at:      0     119     256     620    1011 
   Size:        119     137     364     391

  Fragments arranged by size: 

                 391     364     137     119
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     842     874    1011 
   Size:        842      32     137

  Fragments arranged by size: 

                 842     137      32
 
XmnI GAAnn'nnTTC

Cuts at:      0     765     995    1011 
   Size:        765     230      16

  Fragments arranged by size: 

                 765     230      16
 
ZraI GAC'GTC

Cuts at:      0     945    1011 
   Size:        945      66
 

 Enzymes that do cut:

    AatII     AccI     AcuI     AhdI    AlwNI     BbsI    BbvCI     BglI
     BlpI     BmrI     BpmI   Bpu10I    BsaHI    BsaWI    BseRI    BseYI
     BsgI    BsiEI  BsiHKAI Bsp1286I    BspEI    BspMI    BsrBI    BsrGI
    BssSI   BstEII    BstXI     BtgI    BtgZI     BtsI     DraI     EaeI
     EagI     EarI  Eco57MI EcoO109I    Hin4I     MmeI     MslI   MspA1I
    PpuMI    PvuII    SacII     StuI     StyI    TaqII     TatI     TstI
     XmnI     ZraI

 Enzymes that do not cut: 

     AarI   Acc65I     AclI     AfeI    AflII   AflIII     AgeI     AleI
     AlfI     AloI     ApaI    ApaLI     ApoI     AscI     AseI    AsiSI
     AvaI    AvrII     BaeI    BamHI     BanI    BanII     BbeI     BcgI
    BciVI     BclI    BfrBI    BglII Bme1580I    BmgBI     BmtI     BplI
    BpuEI     BsaI    BsaAI    BsaBI    BsaXI    BsiWI     BsmI    BsmBI
    BspHI    BsrDI    BsrFI   BssHII   BstAPI    BstBI    BstYI  BstZ17I
   Bsu36I     ClaI    CspCI   DraIII     DrdI     EciI  EcoICRI    EcoNI
    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII   HincII
  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MscI     NaeI
     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     NspI     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI
     PpiI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI
    RsrII     SacI     SalI    SanDI     SapI     SbfI     ScaI    SexAI
     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI
     SpeI     SphI     SrfI     SspI     SwaI  Tth111I     XbaI     XcmI
     XhoI     XmaI
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