Restriction map for pF1KB6330
##################################
# NO CUT ENZYMES (  132 enzymes) #
##################################

Acc65I      AccI        AccIII      AclI        AcsI        
AflII       AflIII      AgeI        AhdI        Alw44I      
Aor51HI     ApaI        ApaLI       ApoI        AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AviII       AvrII       BalI        BanI        BbeI        
BbrPI       BbvCI       BcgI        BciVI       BfrI        
BlnI        BsaAI       BsaBI       BsaI        BsaWI       
BseAI       BsiEI       BsiWI       BsmBI       BsmI        
Bsp1407I    BspDI       BspEI       BspHI       BspLU11I    
BsrDI       BsrGI       BssHII      BssSI       Bst1107I    
BstBI       BstZ17I     CfoI        ClaI        CpoI        
DraI        DrdI        EagI        Eam1105I    EarI        
EclXI       Eco47III    Eco52I      EcoRI       EcoT22I     
FseI        FspI        HaeII       HhaI        HinP1I      
HincII      HindII      HindIII     HpaI        KasI        
KpnI        Ksp632I     MamI        MfeI        MluI        
MluNI       MroI        MscI        MseI        MunI        
NarI        NdeI        NheI        NotI        NruI        
NsiI        NspI        NspV        PacI        PaeR7I      
PinAI       PmaCI       PmeI        PmlI        PshAI       
PshBI       Psp1406I    PvuI        RcaI        RsrII       
SalI        SapI        SbfI        ScaI        SexAI       
SfiI        SfoI        SfuI        SgrAI       SmaI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SspI        StuI        SwaI        TaqI        TfiI        
Tru9I       TthHB8I     XbaI        XhoI        XmaCI       
XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   50 enzymes) #
###################################

AatII         751  GACGTC               GACGT^C
AccII         784  CGCG                 CG^CG
AcyI          751  GACGTC               G[GA]^CG[CT]C
AocI          435  CCTCAGG              CC^T[ACGT]AGG
AspI          115  GACAAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaI           97  CTCGGG               C^[CT]CG[GA]G
BamHI         212  GGATCC               G^GATCC
BbsI          844  GTCTTC               GAAGAC(2/6)
BclI          245  TGATCA               T^GATCA
BglI          584  GCCAAATCGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII         473  AGATCT               A^GATCT
BpmI          757  CTGGAG               CTGGAG(16/14)
BpuAI         844  GTCTTC               GAAGAC(2/6)
BsaHI         751  GACGTC               G[GA]^CG[CT]C
BsgI          331  GTGCAG               GTGCAG(16/14)
BsmAI         637  GTCTC                GTCTC(1/5)
BsoBI          97  CTCGGG               C^[CT]CG[GA]G
BsrBI         696  CCGCTC               CCGCTC(-3/-3)
BsrFI         320  GCCGGC               [GA]^CCGG[CT]
BstAPI        276  GCAGGTGCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII        360  GGTCACC              G^GT[ACGT]ACC
BstPI         360  GGTCACC              G^GT[ACGT]ACC
BstUI         784  CGCG                 CG^CG
BstXI         390  CCAGTTGAGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I        435  CCTCAGG              CC^T[ACGT]AGG
Cfr10I        320  GCCGGC               [GA]^CCGG[CT]
DraIII        608  CACTGGGTG            CAC[ACGT][ACGT][ACGT]^GTG
Eco81I        435  CCTCAGG              CC^T[ACGT]AGG
EcoNI          33  CCTTCAGGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I       360  GGTCACC              G^GT[ACGT]ACC
EcoRV         724  GATATC               GAT^ATC
FbaI          245  TGATCA               T^GATCA
HgaI          576  GCGTC                GACGC(5/10)
Hin1I         751  GACGTC               G[GA]^CG[CT]C
KspI          783  CCGCGG               CCGC^GG
MslI          369  CATCCAGGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI          784  CGCG                 CG^CG
NaeI          320  GCCGGC               GCC^GGC
NcoI          356  CCATGG               C^CATGG
NgoMIV        320  GCCGGC               G^CCGGC
PflFI         115  GACAAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PstI           76  CTGCAG               CTGCA^G
PvuII          82  CAGCTG               CAG^CTG
SacI           94  GAGCTC               GAGCT^C
SacII         783  CCGCGG               CCGC^GG
SfaNI          16  GATGC                GCATC(5/9)
SfcI           76  CTGCAG               C^T[GA][CT]AG
SmlI           55  CTCAAG               C^T[CT][GA]AG
Tsp509I       205  AATT                 ^AATT
Tth111I       115  GACAAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC


###################################
# TWO CUT ENZYMES (   16 enzymes) #
###################################

AfaI          703  GTAC                 GT^AC
              817  GTAC                
BanII          94  GAGCTC               G[GA]GC[CT]^C
              234  GGGCTC              
BcnI          177  CCCGG                CC^[GC]GG
              738  CCCGG               
BlpI          271  GCTGAGC              GC^T[ACGT]AGC
              571  GCTCAGC             
Bpu1102I      271  GCTGAGC              GC^T[ACGT]AGC
              571  GCTCAGC             
CelII         271  GCTGAGC              GC^T[ACGT]AGC
              571  GCTCAGC             
DsaI          356  CCATGG               C^C[GA][CT]GG
              783  CCGCGG              
EaeI          399  TGGCCG               [CT]^GGCC[GA]
              591  CGGCCA              
MaeII         515  ACGT                 A^CGT
              752  ACGT                
MaeIII        361  GTCAC                ^GT[ACGT]AC
              415  GTCAC               
MboII         476  TCTTC                GAAGA(8/7)
              845  TCTTC               
MspA1I         82  CAGCTG               C[AC]G^C[GT]G
              783  CCGCGG              
NciI          177  CCCGG                CC^[GC]GG
              738  CCCGG               
RsaI          703  GTAC                 GT^AC
              817  GTAC                
Tsp45I        361  GTCAC                ^GT[GC]AC
              415  GTCAC               
XcmI          137  CCAAGATCCCCCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
              368  CCATCCAGGTGATGG     


#########################################
# 3 OR MORE CUT ENZYMES (   60 enzymes) #
#########################################

AciI        AluI        AlwI        AlwNI       AspHI       
AvaII       BbvI        BfaI        BmyI        BsaJI       
BseRI       BsiHKAI     BsiYI       BslI        BsmFI       
Bsp1286I    BspMI       BsrI        BssKI       BstF5I      
BstNI       BstYI       Cac8I       Cfr13I      DdeI        
DpnII       DraII       EcoO109I    EcoRII      EcoT14I     
FauI        Fnu4HI      FokI        HaeIII      HapII       
HinfI       HpaII       HphI        ItaI        MaeI        
MboI        MflI        MnlI        MspI        MvaI        
MwoI        NdeII       NlaIII      NlaIV       PflMI       
PleI        PpuMI       Sau3AI      Sau96I      ScrFI       
StyI        TseI        TspRI       Van91I      XhoII       
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