Restriction map for pF1KB6330
 (Linear) (Six-base) MAPSORT of: KIBB6330.seq  Check: 9414  from: 1  to: 864



KIBB6330.orf

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:46  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     267     864 
   Size:        267     597
 
AatII G_ACGT'C

Cuts at:      0     755     864 
   Size:        755     109
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      19     864 
   Size:         19     845
 
AlwNI CAG_nnn'CTG

Cuts at:      0      84     282     579     864 
   Size:         84     198     297     285

  Fragments arranged by size: 

                 297     285     198      84
 
AvaI C'yCGr_G

Cuts at:      0      97     864 
   Size:         97     767
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     800     833     864 
   Size:        800      33      31

  Fragments arranged by size: 

                 800      33      31
 
BamHI G'GATC_C

Cuts at:      0     212     864 
   Size:        212     652
 
BanII G_rGCy'C

Cuts at:      0      98     238     864 
   Size:         98     140     626

  Fragments arranged by size: 

                 626     140      98
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     837     864 
   Size:        837      27
 
BclI T'GATC_A

Cuts at:      0     245     864 
   Size:        245     619
 
BglI GCCn_nnn'nGGC

Cuts at:      0     590     864 
   Size:        590     274
 
BglII A'GATC_T

Cuts at:      0     473     864 
   Size:        473     391
 
BlpI GC'TnA_GC

Cuts at:      0     272     572     864 
   Size:        272     300     292

  Fragments arranged by size: 

                 300     292     272
 
Bme1580I G_kGCm'C

Cuts at:      0     137     864 
   Size:        137     727
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     619     864 
   Size:        619     245
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     656     864 
   Size:        656     208
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     778     864 
   Size:        778      86
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      40     864 
   Size:         40     824
 
BsaHI Gr'CG_yC

Cuts at:      0     752     864 
   Size:        752     112
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      55     328     463     466     864 
   Size:         55     273     135       3     398

  Fragments arranged by size: 

                 398     273     135      55       3
 
BseYI C'CCAG_C

Cuts at:      0     167     546     864 
   Size:        167     379     318

  Fragments arranged by size: 

                 379     318     167
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     352     864 
   Size:        352     512
 
BsiHKAI G_wGCw'C

Cuts at:      0      98     299     713     864 
   Size:         98     201     414     151

  Fragments arranged by size: 

                 414     201     151      98
 
Bsp1286I G_dGCh'C

Cuts at:      0      98     137     238     299     713     864 
   Size:         98      39     101      61     414     151

  Fragments arranged by size: 

                 414     151     101      98      61      39
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      83     267     324     864 
   Size:         83     184      57     540

  Fragments arranged by size: 

                 540     184      83      57
 
BsrBI CCG'CTC

Cuts at:      0     698     864 
   Size:        698     166
 
BsrFI r'CCGG_y

Cuts at:      0     320     864 
   Size:        320     544
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     282     864 
   Size:        282     582
 
BstEII G'GTnAC_C

Cuts at:      0     360     864 
   Size:        360     504
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     397     864 
   Size:        397     467
 
BstYI r'GATC_y

Cuts at:      0     140     212     473     659     761     864 
   Size:        140      72     261     186     102     103

  Fragments arranged by size: 

                 261     186     140     103     102      72
 
Bsu36I CC'TnA_GG

Cuts at:      0     436     864 
   Size:        436     428
 
BtgI C'CryG_G

Cuts at:      0     356     783     864 
   Size:        356     427      81

  Fragments arranged by size: 

                 427     356      81
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     521     864 
   Size:        521     343
 
BtsI GCAGTG_nn'

Cuts at:      0     216     864 
   Size:        216     648
 
DraIII CAC_nnn'GTG

Cuts at:      0     613     864 
   Size:        613     251
 
EaeI y'GGCC_r

Cuts at:      0     399     591     864 
   Size:        399     192     273

  Fragments arranged by size: 

                 399     273     192
 
EcoICRI GAG'CTC

Cuts at:      0      96     864 
   Size:         96     768
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      19     778     864 
   Size:         19     759      86

  Fragments arranged by size: 

                 759      86      19
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0      37     864 
   Size:         37     827
 
EcoO109I rG'GnC_Cy

Cuts at:      0      72     120     174     804     834     864 
   Size:         72      48      54     630      30      30

  Fragments arranged by size: 

                 630      72      54      48      30      30
 
EcoRV GAT'ATC

Cuts at:      0     726     864 
   Size:        726     138
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     664     696     864 
   Size:        664      32     168

  Fragments arranged by size: 

                 664     168      32
 
MslI CAynn'nnrTG

Cuts at:      0     373     864 
   Size:        373     491
 
MspA1I CmG'CkG

Cuts at:      0      84     785     864 
   Size:         84     701      79

  Fragments arranged by size: 

                 701      84      79
 
NaeI GCC'GGC

Cuts at:      0     322     864 
   Size:        322     542
 
NcoI C'CATG_G

Cuts at:      0     356     864 
   Size:        356     508
 
NgoMIV G'CCGG_C

Cuts at:      0     320     864 
   Size:        320     544
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     345     378     613     864 
   Size:        345      33     235     251

  Fragments arranged by size: 

                 345     251     235      33
 
PfoI T'CCnGG_A

Cuts at:      0     755     864 
   Size:        755     109
 
PpuMI rG'GwC_Cy

Cuts at:      0      72     120     174     864 
   Size:         72      48      54     690

  Fragments arranged by size: 

                 690      72      54      48
 
PstI C_TGCA'G

Cuts at:      0      80     864 
   Size:         80     784
 
PvuII CAG'CTG

Cuts at:      0      84     864 
   Size:         84     780
 
SacI G_AGCT'C

Cuts at:      0      98     864 
   Size:         98     766
 
SacII CC_GC'GG

Cuts at:      0     786     864 
   Size:        786      78
 
SfcI C'TryA_G

Cuts at:      0      76     864 
   Size:         76     788
 
SmlI C'TyrA_G

Cuts at:      0      55     864 
   Size:         55     809
 
StyI C'CwwG_G

Cuts at:      0     344     356     822     864 
   Size:        344      12     466      42

  Fragments arranged by size: 

                 466     344      42      12
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     181     601     864 
   Size:        181     420     263

  Fragments arranged by size: 

                 420     263     181
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     408     440     486     518     864 
   Size:        408      32      46      32     346

  Fragments arranged by size: 

                 408     346      46      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     118     864 
   Size:        118     746
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     144     375     864 
   Size:        144     231     489

  Fragments arranged by size: 

                 489     231     144
 
ZraI GAC'GTC

Cuts at:      0     753     864 
   Size:        753     111
 

 Enzymes that do cut:

     AarI    AatII     AcuI    AlwNI     AvaI     BaeI    BamHI    BanII
     BbsI     BclI     BglI    BglII     BlpI Bme1580I     BmrI     BplI
     BpmI    BpuEI    BsaHI    BseRI    BseYI     BsgI  BsiHKAI Bsp1286I
    BspMI    BsrBI    BsrFI   BstAPI   BstEII    BstXI    BstYI   Bsu36I
     BtgI    BtgZI     BtsI   DraIII     EaeI  EcoICRI  Eco57MI    EcoNI
 EcoO109I    EcoRV    Hin4I     MslI   MspA1I     NaeI     NcoI   NgoMIV
    PflMI     PfoI    PpuMI     PstI    PvuII     SacI    SacII     SfcI
     SmlI     StyI    TaqII     TstI  Tth111I     XcmI     ZraI

 Enzymes that do not cut: 

     AccI   Acc65I     AclI     AfeI    AflII   AflIII     AgeI     AhdI
     AleI     AlfI     AloI     ApaI    ApaLI     ApoI     AscI     AseI
    AsiSI    AvrII     BanI     BbeI    BbvCI     BcgI    BciVI    BfrBI
    BmgBI     BmtI   Bpu10I     BsaI    BsaAI    BsaBI    BsaWI    BsaXI
    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BsrDI    BsrGI
   BssHII    BssSI    BstBI  BstZ17I     ClaI    CspCI     DraI     DrdI
     EagI     EarI     EciI    EcoRI     FalI     FseI     FspI    FspAI
    HaeII   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI
     MmeI     MscI     NarI     NdeI     NheI     NotI     NruI     NsiI
     NspI     PacI     PasI     PciI     PmeI     PmlI     PpiI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PvuI    RsrII     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI
     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI
     TatI     XbaI     XhoI     XmaI     XmnI
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