Restriction map for pF1KB8870
##################################
# NO CUT ENZYMES (  181 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AfaI        AflII       AflIII      AgeI        
AhdI        Alw44I      AlwI        AlwNI       AocI        
Aor51HI     ApaI        ApaLI       AscI        AseI        
AsnI        Asp718I     AspEI       AspHI       AspI        
AviII       AvrII       BalI        BamHI       BanII       
BbeI        BbrPI       BbsI        BbvCI       BciVI       
BclI        BfaI        BfrI        BglI        BglII       
BlnI        BlpI        BpmI        Bpu1102I    BpuAI       
BsaAI       BsaWI       BseAI       BseRI       BsgI        
BsiEI       BsiHKAI     BsiWI       BsmBI       BsmI        
Bsp1407I    BspDI       BspEI       BspHI       BspLU11I    
BsrBI       BsrDI       BsrFI       BsrGI       BsrI        
BssHII      BssSI       Bst1107I    BstAPI      BstBI       
BstEII      BstPI       BstUI       BstXI       BstYI       
BstZ17I     Bsu36I      CelII       CfoI        Cfr10I      
ClaI        CpoI        DraI        DraII       DrdI        
DsaI        EaeI        EagI        Eam1105I    EarI        
EclXI       Eco47III    Eco52I      Eco81I      EcoNI       
EcoO109I    EcoO65I     EcoT14I     EcoT22I     FauI        
FbaI        FseI        FspI        HaeII       HaeIII      
HhaI        HinP1I      HincII      HindII      HindIII     
HpaI        KasI        KpnI        Ksp632I     KspI        
MaeI        MaeIII      MboII       MfeI        MflI        
MluI        MluNI       MroI        MscI        MslI        
MunI        MvnI        NaeI        NarI        NcoI        
NdeI        NgoMIV      NheI        NotI        NruI        
NsiI        NspI        NspV        PacI        PaeR7I      
PflFI       PinAI       PmaCI       PmeI        PmlI        
PpuMI       PshAI       PshBI       Psp1406I    PvuI        
PvuII       RcaI        RsaI        RsrII       SacI        
SacII       SalI        SapI        SbfI        ScaI        
SexAI       SfiI        SfoI        SfuI        SgrAI       
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SspI        StuI        StyI        SwaI        TfiI        
Tsp45I      Tth111I     XbaI        XcmI        XhoI        
XhoII       


###################################
# ONE CUT ENZYMES (   46 enzymes) #
###################################

AciI          304  CCGC                 CCGC(-3/-1)
AcsI          355  GAATTC               [GA]^AATT[CT]
ApoI          355  GAATTC               [GA]^AATT[CT]
Asp700I         3  GAAAGCCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI          277  CCCGGG               C^[CT]CG[GA]G
BanI          147  GGTGCC               G^G[CT][GA]CC
BbvI          195  GCAGC                GCAGC(8/12)
BcgI           95  CGATGAGCCTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BmyI          148  GTGCCC               G[AGT]GC[ACT]^C
BsaBI         183  GATGGAAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI          273  GAGACC               GGTCTC(1/5)
BsmAI         273  GAGAC                GTCTC(1/5)
BsmFI          14  GTCCC                GGGAC(10/14)
BsoBI         277  CCCGGG               C^[CT]CG[GA]G
Bsp1286I      148  GTGCCC               G[AGT]GC[ACT]^C
BspMI         221  ACCTGC               ACCTGC(4/8)
DdeI          365  CTGAG                C^T[ACGT]AG
DpnII         228  GATC                 ^GATC
DraIII        392  CACTGTGTG            CAC[ACGT][ACGT][ACGT]^GTG
EcoRI         355  GAATTC               G^AATTC
EcoRV         325  GATATC               GAT^ATC
HinfI         238  GACTC                G^A[ACGT]TC
MaeII         200  ACGT                 A^CGT
MamI          183  GATGGAAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MboI          228  GATC                 ^GATC
MseI          370  TTAA                 T^TAA
MspA1I        304  CCGCTG               C[AC]G^C[GT]G
MwoI          135  GCTCAAGGAGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NdeII         228  GATC                 ^GATC
NlaIII        114  CATG                 CATG^
NlaIV         147  GGTGCC               GG[ACGT]^[ACGT]CC
PflMI          51  CCACAGCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI          238  GACTC                GAGTC(4/5)
Sau3AI        228  GATC                 ^GATC
SmaI          277  CCCGGG               CCC^GGG
SmlI          136  CTCAAG               C^T[CT][GA]AG
TaqI          206  TCGA                 T^CGA
Tru9I         370  TTAA                 T^TAA
TseI          195  GCAGC                G^C[AT]GC
Tsp509I       356  AATT                 ^AATT
TspRI         392  CACTG                CA[GC]TG[ACGT][ACGT]^
TthHB8I       206  TCGA                 T^CGA
Van91I         51  CCACAGCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI         277  CCCGGG               C^CCGGG
XmaI          277  CCCGGG               C^CCGGG
XmnI            3  GAAAGCCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   16 enzymes) #
###################################

AcyI          291  GGCGTC               G[GA]^CG[CT]C
              300  GACGCC              
AluI          134  AGCT                 AG^CT
              143  AGCT                
BsaHI         291  GGCGTC               G[GA]^CG[CT]C
              300  GACGCC              
BsiYI          51  CCACAGCCTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              315  CCTCAACACGG         
BslI           51  CCACAGCCTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              315  CCTCAACACGG         
Fnu4HI        195  GCAGC                GC^[ACGT]GC
              303  GCCGC               
HapII          69  CCGG                 C^CGG
              278  CCGG                
HgaI          300  GACGC                GACGC(5/10)
              292  GCGTC               
Hin1I         291  GGCGTC               G[GA]^CG[CT]C
              300  GACGCC              
HpaII          69  CCGG                 C^CGG
              278  CCGG                
HphI          174  GGTGA                GGTGA(8/7)
              267  TCACC               
ItaI          195  GCAGC                GC^[ACGT]GC
              303  GCCGC               
MnlI          315  CCTC                 CCTC(7/6)
               21  GAGG                
MspI           69  CCGG                 C^CGG
              278  CCGG                
PstI          193  CTGCAG               CTGCA^G
              223  CTGCAG              
SfcI          193  CTGCAG               C^T[GA][CT]AG
              223  CTGCAG              


#########################################
# 3 OR MORE CUT ENZYMES (   15 enzymes) #
#########################################

AvaII       BcnI        BsaJI       BssKI       BstF5I      
BstNI       Cac8I       Cfr13I      EcoRII      FokI        
MvaI        NciI        Sau96I      ScrFI       SfaNI       
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