Restriction map for pF1KB6389
##################################
# NO CUT ENZYMES (  135 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcyI        AflII       AgeI        Alw44I      AocI        
Aor51HI     ApaI        ApaLI       AscI        AseI        
AsnI        Asp718I     AspHI       AviII       AvrII       
BamHI       BbeI        BbsI        BbvCI       BcgI        
BciVI       BclI        BfrI        BlnI        BlpI        
Bpu1102I    BpuAI       BsaHI       BsaWI       BseAI       
BsiEI       BsiHKAI     BsiWI       BsmBI       BsmI        
Bsp1407I    BspDI       BspEI       BspHI       BspLU11I    
BsrFI       BsrGI       BsrI        BssHII      BssSI       
Bst1107I    BstBI       BstEII      BstPI       BstZ17I     
Bsu36I      CelII       Cfr10I      ClaI        CpoI        
DraI        DraIII      DrdI        EagI        EclXI       
Eco47III    Eco52I      Eco81I      EcoNI       EcoO65I     
EcoRV       EcoT22I     FauI        FbaI        FseI        
FspI        HaeII       HgaI        Hin1I       HindIII     
HpaI        KasI        KpnI        KspI        MfeI        
MluI        MroI        MseI        MunI        NaeI        
NarI        NcoI        NdeI        NgoMIV      NheI        
NotI        NruI        NsiI        NspI        NspV        
PacI        PflMI       PinAI       PmeI        PshBI       
Psp1406I    PvuI        PvuII       RcaI        RsrII       
SacI        SacII       SalI        SapI        SbfI        
ScaI        SexAI       SfaNI       SfiI        SfoI        
SfuI        SgrAI       SmaI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        SwaI        
Tru9I       Van91I      XcmI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   63 enzymes) #
###################################

AccII         595  CGCG                 CG^CG
AcsI          306  GAATTC               [GA]^AATT[CT]
AflIII         87  ACACGT               A^C[GA][CT]GT
AhdI          583  GACTTGATGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AluI          104  AGCT                 AG^CT
AlwI           61  GGATC                GGATC(4/5)
AlwNI         491  CAGCCCCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApoI          306  GAATTC               [GA]^AATT[CT]
Asp700I        72  GAAGGTATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI         583  GACTTGATGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI          256  GACACGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaI          130  CTCGAG               C^[CT]CG[GA]G
BalI          549  TGGCCA               TGG^CCA
BbrPI          88  CACGTG               CAC^GTG
BcnI          287  CCGGG                CC^[GC]GG
BfaI          190  CTAG                 C^TAG
BglI          330  GCCTCCAAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII         641  AGATCT               A^GATCT
BpmI          178  CTGGAG               CTGGAG(16/14)
BsaAI          88  CACGTG               [CT]AC^GT[GA]
BsaBI         669  GATGGTCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI         798  GAGGAG               GAGGAG(10/8)
BsoBI         130  CTCGAG               C^[CT]CG[GA]G
BsrBI         788  GAGCGG               CCGCTC(-3/-3)
BsrDI         455  GCAATG               GCAATG(2/0)
BstAPI        490  GCAGCCCCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI         595  CGCG                 CG^CG
BstXI         362  CCACTTTGGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI         641  AGATCT               [GA]^GATC[CT]
CfoI          756  GCGC                 GCG^C
Eam1105I      583  GACTTGATGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI         306  GAATTC               G^AATTC
EcoT14I       334  CCAAGG               C^C[AT][AT]GG
HhaI          756  GCGC                 GCG^C
HinP1I        756  GCGC                 G^CGC
HincII        136  GTCAAC               GT[CT]^[GA]AC
HindII        136  GTCAAC               GT[CT]^[GA]AC
MaeI          190  CTAG                 C^TAG
MamI          669  GATGGTCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI          641  AGATCT               [GA]^GATC[CT]
MluNI         549  TGGCCA               TGG^CCA
MscI          549  TGGCCA               TGG^CCA
MspA1I        763  CAGCGG               C[AC]G^C[GT]G
MvnI          595  CGCG                 CG^CG
NciI          287  CCGGG                CC^[GC]GG
NlaIII        253  CATG                 CATG^
PaeR7I        130  CTCGAG               C^TCGAG
PflFI         256  GACACGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI          88  CACGTG               CAC^GTG
PmlI           88  CACGTG               CAC^GTG
PshAI         717  GACATCTGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI          141  CTGCAG               CTGCA^G
SfcI          141  CTGCAG               C^T[GA][CT]AG
SmlI          130  CTCGAG               C^T[CT][GA]AG
StuI          812  AGGCCT               AGG^CCT
StyI          334  CCAAGG               C^C[AT][AT]GG
TfiI          198  GATTC                G^A[AT]TC
Tsp509I       307  AATT                 ^AATT
Tth111I       256  GACACGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XbaI          189  TCTAGA               T^CTAGA
XhoI          130  CTCGAG               C^TCGAG
XhoII         641  AGATCT               [GA]^GATC[CT]
XmnI           72  GAAGGTATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   24 enzymes) #
###################################

AfaI           85  GTAC                 GT^AC
              324  GTAC                
BanI          405  GGTGCC               G^G[CT][GA]CC
              772  GGCACC              
BanII         115  GAGCCC               G[GA]GC[CT]^C
              418  GAGCCC              
BsgI          466  CTGCAC               GTGCAG(16/14)
              399  GTGCAG              
BsmFI         126  GTCCC                GGGAC(10/14)
              705  GTCCC               
BspMI         280  ACCTGC               ACCTGC(4/8)
              339  GCAGGT              
DdeI          218  CTCAG                C^T[ACGT]AG
              621  CTCAG               
DraII          19  AGGACCC              [GA]G^G[ACGT]CC[CT]
              124  GGGTCCC             
DsaI          413  CCGTGG               C^C[GA][CT]GG
              450  CCGTGG              
EarI           37  CTCTTC               CTCTTC(1/4)
              439  CTCTTC              
EcoO109I       19  AGGACCC              [GA]G^G[ACGT]CC[CT]
              124  GGGTCCC             
HapII         287  CCGG                 C^CGG
              819  CCGG                
HpaII         287  CCGG                 C^CGG
              819  CCGG                
Ksp632I        37  CTCTTC               CTCTTC(1/4)
              439  CTCTTC              
MaeII          89  ACGT                 A^CGT
              314  ACGT                
MslI          363  CACTTTGGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              684  CACGGTGGTG          
MspI          287  CCGG                 C^CGG
              819  CCGG                
PleI          462  GACTC                GAGTC(4/5)
              298  GAGTC               
PpuMI          19  AGGACCC              [GA]G^G[AT]CC[CT]
              124  GGGTCCC             
RsaI           85  GTAC                 GT^AC
              324  GTAC                
TaqI           80  TCGA                 T^CGA
              131  TCGA                
Tsp45I        682  GTCAC                ^GT[GC]AC
              715  GTGAC               
TspRI         220  CAGTG                CA[GC]TG[ACGT][ACGT]^
              353  CACTG               
TthHB8I        80  TCGA                 T^CGA
              131  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   36 enzymes) #
#########################################

AciI        AvaII       BbvI        BmyI        BsaI        
BsaJI       BsiYI       BslI        BsmAI       Bsp1286I    
BssKI       BstF5I      BstNI       Cac8I       Cfr13I      
DpnII       EaeI        EcoRII      Fnu4HI      FokI        
HaeIII      HinfI       HphI        ItaI        MaeIII      
MboI        MboII       MnlI        MvaI        MwoI        
NdeII       NlaIV       Sau3AI      Sau96I      ScrFI       
TseI        
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