Restriction map for pF1KB6389
 (Linear) (Six-base) MAPSORT of: KIBB6389.seq  Check: 8973  from: 1  to: 825



KIBB6389.orf

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:46  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     289     825 
   Size:        289     536
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     185     825 
   Size:        185     640
 
AflIII A'CryG_T

Cuts at:      0      87     825 
   Size:         87     738
 
AhdI GACnn_n'nnGTC

Cuts at:      0     588     825 
   Size:        588     237
 
AleI CACnn'nnGTG

Cuts at:      0     367     688     825 
   Size:        367     321     137

  Fragments arranged by size: 

                 367     321     137
 
AlwNI CAG_nnn'CTG

Cuts at:      0     496     825 
   Size:        496     329
 
ApoI r'AATT_y

Cuts at:      0     306     825 
   Size:        306     519
 
AvaI C'yCGr_G

Cuts at:      0     130     825 
   Size:        130     695
 
BanI G'GyrC_C

Cuts at:      0     405     772     825 
   Size:        405     367      53

  Fragments arranged by size: 

                 405     367      53
 
BanII G_rGCy'C

Cuts at:      0     119     422     825 
   Size:        119     303     403

  Fragments arranged by size: 

                 403     303     119
 
BglI GCCn_nnn'nGGC

Cuts at:      0     336     825 
   Size:        336     489
 
BglII A'GATC_T

Cuts at:      0     641     825 
   Size:        641     184
 
Bme1580I G_kGCm'C

Cuts at:      0     410     775     825 
   Size:        410     365      50

  Fragments arranged by size: 

                 410     365      50
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     199     825 
   Size:        199     626
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     175     243     469     825 
   Size:        175      68     226     356

  Fragments arranged by size: 

                 356     226     175      68
 
BsaAI yAC'GTr

Cuts at:      0      90     825 
   Size:         90     735
 
BsaBI GATnn'nnATC

Cuts at:      0     673     825 
   Size:        673     152
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     813     825 
   Size:        813      12
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     420     451     825 
   Size:        420      31     374

  Fragments arranged by size: 

                 420     374      31
 
Bsp1286I G_dGCh'C

Cuts at:      0     119     410     422     775     825 
   Size:        119     291      12     353      50

  Fragments arranged by size: 

                 353     291     119      50      12
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     289     330     825 
   Size:        289      41     495

  Fragments arranged by size: 

                 495     289      41
 
BsrBI CCG'CTC

Cuts at:      0     790     825 
   Size:        790      35
 
BsrDI GCAATG_nn'

Cuts at:      0     462     825 
   Size:        462     363
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     496     825 
   Size:        496     329
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     369     825 
   Size:        369     456
 
BstYI r'GATC_y

Cuts at:      0     641     825 
   Size:        641     184
 
BtgI C'CryG_G

Cuts at:      0     413     450     825 
   Size:        413      37     375

  Fragments arranged by size: 

                 413     375      37
 
BtsI GCAGTG_nn'

Cuts at:      0     352     825 
   Size:        352     473
 
EaeI y'GGCC_r

Cuts at:      0     549     744     820     825 
   Size:        549     195      76       5

  Fragments arranged by size: 

                 549     195      76       5
 
EarI CTCTTCn'nnn_

Cuts at:      0      43     445     825 
   Size:         43     402     380

  Fragments arranged by size: 

                 402     380      43
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     185     199     825 
   Size:        185      14     626

  Fragments arranged by size: 

                 626     185      14
 
EcoO109I rG'GnC_Cy

Cuts at:      0      20     125     825 
   Size:         20     105     700

  Fragments arranged by size: 

                 700     105      20
 
EcoRI G'AATT_C

Cuts at:      0     306     825 
   Size:        306     519
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      39      71     574     606     640     672     825 
   Size:         39      32     503      32      34      32     153

  Fragments arranged by size: 

                 503     153      39      34      32      32      32
 
HincII GTy'rAC

Cuts at:      0     138     825 
   Size:        138     687
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     335     617     638     825 
   Size:        335     282      21     187

  Fragments arranged by size: 

                 335     282     187      21
 
MscI TGG'CCA

Cuts at:      0     551     825 
   Size:        551     274
 
MslI CAynn'nnrTG

Cuts at:      0     367     688     825 
   Size:        367     321     137

  Fragments arranged by size: 

                 367     321     137
 
MspA1I CmG'CkG

Cuts at:      0     765     825 
   Size:        765      60
 
PfoI T'CCnGG_A

Cuts at:      0      57     286     825 
   Size:         57     229     539

  Fragments arranged by size: 

                 539     229      57
 
PmlI CAC'GTG

Cuts at:      0      90     825 
   Size:         90     735
 
PpuMI rG'GwC_Cy

Cuts at:      0      20     125     825 
   Size:         20     105     700

  Fragments arranged by size: 

                 700     105      20
 
PshAI GACnn'nnGTC

Cuts at:      0     721     825 
   Size:        721     104
 
PspXI vC'TCGA_Gb

Cuts at:      0     130     825 
   Size:        130     695
 
PstI C_TGCA'G

Cuts at:      0     145     825 
   Size:        145     680
 
SanDI GG'GwC_CC

Cuts at:      0     125     825 
   Size:        125     700
 
SfcI C'TryA_G

Cuts at:      0     141     825 
   Size:        141     684
 
SmlI C'TyrA_G

Cuts at:      0     130     825 
   Size:        130     695
 
StuI AGG'CCT

Cuts at:      0     814     825 
   Size:        814      11
 
StyI C'CwwG_G

Cuts at:      0     334     825 
   Size:        334     491
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     160     825 
   Size:        160     665
 
Tth111I GACn'n_nGTC

Cuts at:      0     259     825 
   Size:        259     566
 
XbaI T'CTAG_A

Cuts at:      0     189     825 
   Size:        189     636
 
XhoI C'TCGA_G

Cuts at:      0     130     825 
   Size:        130     695
 
XmnI GAAnn'nnTTC

Cuts at:      0      76     825 
   Size:         76     749
 

 Enzymes that do cut:

     AarI     AcuI   AflIII     AhdI     AleI    AlwNI     ApoI     AvaI
     BanI    BanII     BglI    BglII Bme1580I     BpmI     BsaI    BsaAI
    BsaBI    BseRI     BsgI Bsp1286I    BspMI    BsrBI    BsrDI   BstAPI
    BstXI    BstYI     BtgI     BtsI     EaeI     EarI  Eco57MI EcoO109I
    EcoRI    Hin4I   HincII     MmeI     MscI     MslI   MspA1I     PfoI
     PmlI    PpuMI    PshAI    PspXI     PstI    SanDI     SfcI     SmlI
     StuI     StyI    TaqII  Tth111I     XbaI     XhoI     XmnI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI     AlfI
     AloI     ApaI    ApaLI     AscI     AseI    AsiSI    AvrII     BaeI
    BamHI     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI
     BlpI    BmgBI     BmrI     BmtI     BplI   Bpu10I    BpuEI    BsaHI
    BsaWI    BsaXI    BseYI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI
    BspEI    BspHI    BsrFI    BsrGI   BssHII    BssSI    BstBI   BstEII
  BstZ17I   Bsu36I    BtgZI     ClaI    CspCI     DraI   DraIII     DrdI
     EagI     EciI  EcoICRI    EcoNI    EcoRV     FalI     FseI     FspI
    FspAI    HaeII  HindIII     HpaI     KasI     KpnI     MfeI     MluI
     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     NspI     PacI     PasI     PciI    PflMI     PmeI     PpiI
     PsiI   PspOMI     PsrI     PvuI    PvuII    RsrII     SacI    SacII
     SalI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI
    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     SwaI
     TatI     TstI     XcmI     XmaI     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com