Restriction map for pF1KB6308
##################################
# NO CUT ENZYMES (  153 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AfaI        AflII       AhdI        
AocI        Aor51HI     ApaI        AscI        AseI        
AsnI        Asp718I     AspEI       AspI        AviII       
BalI        BamHI       BanI        BanII       BbeI        
BbrPI       BbvCI       BbvI        BcgI        BciVI       
BclI        BcnI        BfrI        BglI        BlpI        
BpmI        Bpu1102I    BsaAI       BsaBI       BsaHI       
BseAI       BsiEI       BsiWI       BsmBI       Bsp1407I    
BspDI       BspEI       BspLU11I    BsrBI       BsrDI       
BsrGI       BssHII      BssSI       Bst1107I    BstAPI      
BstBI       BstEII      BstPI       BstUI       BstZ17I     
Bsu36I      CelII       ClaI        CpoI        DdeI        
DraIII      DrdI        EaeI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      Eco81I      EcoNI       
EcoO65I     EcoRI       EcoRV       EcoT22I     FauI        
FbaI        FseI        FspI        HaeII       HgaI        
Hin1I       HincII      HindII      HpaI        KasI        
KpnI        KspI        MamI        MfeI        MluI        
MluNI       MroI        MscI        MspA1I      MunI        
MvnI        NaeI        NarI        NciI        NcoI        
NdeI        NgoMIV      NheI        NotI        NruI        
NsiI        NspV        PacI        PflFI       PflMI       
PleI        PmaCI       PmeI        PmlI        PpuMI       
PshAI       PshBI       Psp1406I    PvuI        PvuII       
RsaI        RsrII       SacI        SacII       SalI        
SapI        SbfI        ScaI        SexAI       SfiI        
SfoI        SfuI        SgrAI       SmaI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SwaI        
TseI        TspRI       Tth111I     Van91I      XbaI        
XcmI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   63 enzymes) #
###################################

AflIII        806  ACGTGT               A^C[GA][CT]GT
AgeI          444  ACCGGT               A^CCGGT
Alw44I        688  GTGCAC               G^TGCAC
AlwI          457  GATCC                GGATC(4/5)
AlwNI         455  CAGATCCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApaLI         688  GTGCAC               G^TGCAC
AspHI         688  GTGCAC               G[AT]GC[AT]^C
AvaI          266  CTCGAG               C^[CT]CG[GA]G
AvaII         450  GGTCC                G^G[AT]CC
AvrII         415  CCTAGG               C^CTAGG
BglII         618  AGATCT               A^GATCT
BlnI          415  CCTAGG               C^CTAGG
BmyI          688  GTGCAC               G[AGT]GC[ACT]^C
BsaI          342  GGTCTC               GGTCTC(1/5)
BsaWI         444  ACCGGT               [AT]^CCGG[AT]
BseRI         149  GAGGAG               GAGGAG(10/8)
BsgI           87  CTGCAC               GTGCAG(16/14)
BsiHKAI       688  GTGCAC               G[AT]GC[AT]^C
BsiYI         399  CCCCATAAAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI          399  CCCCATAAAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmI          288  GAATGC               GAATGC(1/-1)
BsoBI         266  CTCGAG               C^[CT]CG[GA]G
Bsp1286I      688  GTGCAC               G[AGT]GC[ACT]^C
BspHI         798  TCATGA               T^CATGA
BspMI          85  ACCTGC               ACCTGC(4/8)
BsrFI         444  ACCGGT               [GA]^CCGG[CT]
BsrI          481  CCAGT                ACTGG(1/-1)
BssKI         293  CCAGG                ^CC[ACGT]GG
BstNI         293  CCAGG                CC^[AT]GG
BstXI          14  CCAAGATCGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI          832  GCGC                 GCG^C
Cfr10I        444  ACCGGT               [GA]^CCGG[CT]
DraI          613  TTTAAA               TTT^AAA
DraII          28  GGGGCCT              [GA]G^G[ACGT]CC[CT]
DsaI          347  CCGTGG               C^C[GA][CT]GG
EarI          498  GAAGAG               CTCTTC(1/4)
EcoO109I       28  GGGGCCT              [GA]G^G[ACGT]CC[CT]
EcoRII        293  CCAGG                ^CC[AT]GG
EcoT14I       415  CCTAGG               C^C[AT][AT]GG
Fnu4HI        377  GCCGC                GC^[ACGT]GC
HapII         445  CCGG                 C^CGG
HhaI          832  GCGC                 GCG^C
HinP1I        832  GCGC                 G^CGC
HpaII         445  CCGG                 C^CGG
ItaI          377  GCCGC                GC^[ACGT]GC
Ksp632I       498  GAAGAG               CTCTTC(1/4)
MslI          402  CATAAAGGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI          445  CCGG                 C^CGG
MvaI          293  CCAGG                CC^[AT]GG
NspI          158  GCATGT               [GA]CATG^[CT]
PaeR7I        266  CTCGAG               C^TCGAG
PinAI         444  ACCGGT               A^CCGGT
PstI          218  CTGCAG               CTGCA^G
RcaI          798  TCATGA               T^CATGA
ScrFI         293  CCAGG                CC^[ACGT]GG
SfaNI          98  GCATC                GCATC(5/9)
SmlI          266  CTCGAG               C^T[CT][GA]AG
SspI          760  AATATT               AAT^ATT
StuI          749  AGGCCT               AGG^CCT
StyI          415  CCTAGG               C^C[AT][AT]GG
TaqI          267  TCGA                 T^CGA
TthHB8I       267  TCGA                 T^CGA
XhoI          266  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   23 enzymes) #
###################################

AciI           96  CCGC                 CCGC(-3/-1)
              378  CCGC                
AcsI          196  GAATTT               [GA]^AATT[CT]
              570  GAATTT              
ApoI          196  GAATTT               [GA]^AATT[CT]
              570  GAATTT              
Asp700I        39  GAACCACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              183  GAACTTCTTC          
BfaI          416  CTAG                 C^TAG
              556  CTAG                
BsmAI         214  GTCTC                GTCTC(1/5)
              343  GTCTC               
BsmFI         451  GTCCC                GGGAC(10/14)
              810  GTCCC               
BstYI         456  AGATCC               [GA]^GATC[CT]
              618  AGATCT              
Cac8I          75  GCTTGC               GC[ACGT]^[ACGT]GC
              138  GCTGGC              
HindIII        73  AAGCTT               A^AGCTT
              112  AAGCTT              
HinfI          53  GAATC                G^A[ACGT]TC
              411  GATTC               
HphI          408  GGTGA                GGTGA(8/7)
              534  TCACC               
MaeI          416  CTAG                 C^TAG
              556  CTAG                
MaeII         668  ACGT                 A^CGT
              806  ACGT                
MflI          456  AGATCC               [GA]^GATC[CT]
              618  AGATCT              
MseI          116  TTAA                 T^TAA
              614  TTAA                
NlaIV          28  GGGGCC               GG[ACGT]^[ACGT]CC
              450  GGTCCC              
SfcI          218  CTGCAG               C^T[GA][CT]AG
              712  CTACAG              
TfiI           53  GAATC                G^A[AT]TC
              411  GATTC               
Tru9I         116  TTAA                 T^TAA
              614  TTAA                
Tsp45I        131  GTGAC                ^GT[GC]AC
              385  GTGAC               
XhoII         456  AGATCC               [GA]^GATC[CT]
              618  AGATCT              
XmnI           39  GAACCACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              183  GAACTTCTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   19 enzymes) #
#########################################

AluI        BbsI        BpuAI       BsaJI       BstF5I      
Cfr13I      DpnII       FokI        HaeIII      MaeIII      
MboI        MboII       MnlI        MwoI        NdeII       
NlaIII      Sau3AI      Sau96I      Tsp509I     
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