Restriction map for pF1KE0217
 (Circular) (Six-base) MAPSORT of: pF1KE0217.seq  Check: 731  from: 1  to: 3629



pF1KE0217  3629 bp

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:11  ..

AccI GT'mk_AC

Cuts at:    287     667     287 
   Size:        380    3249
 
Acc65I G'GTAC_C

Cuts at:    645     645 
   Size:       3629
 
AclI AA'CG_TT

Cuts at:    565    3469     565 
   Size:       2904     725
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1454    1886    2633    2901    1454 
   Size:        432     747     268    2182

  Fragments arranged by size: 

                2182     747     432     268
 
AflIII A'CryG_T

Cuts at:    197     981    2085    3062     197 
   Size:        784    1104     977     764

  Fragments arranged by size: 

                1104     977     784     764
 
AgeI A'CCGG_T

Cuts at:   2017    2017 
   Size:       3629
 
AlwNI CAG_nnn'CTG

Cuts at:    934    2501     934 
   Size:       1567    2062
 
ApaLI G'TGCA_C

Cuts at:   2399    2399 
   Size:       3629
 
ApoI r'AATT_y

Cuts at:    402     488     633    2982    3238     402 
   Size:         86     145    2349     256     793

  Fragments arranged by size: 

                2349     793     256     145      86
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       3629
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3629
 
AvaI C'yCGr_G

Cuts at:    649     649 
   Size:       3629
 
BamHI G'GATC_C

Cuts at:    654     654 
   Size:       3629
 
BanI G'GyrC_C

Cuts at:    645    1059    1307    1342     645 
   Size:        414     248      35    2932

  Fragments arranged by size: 

                2932     414     248      35
 
BanII G_rGCy'C

Cuts at:    181     643    1673     181 
   Size:        462    1030    2137

  Fragments arranged by size: 

                2137    1030     462
 
BbeI G_GCGC'C

Cuts at:   1063    1311    1063 
   Size:        248    3381
 
BbsI GAAGACnn'nnnn_

Cuts at:    344     344 
   Size:       3629
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:    115     149     115 
   Size:         34    3595
 
BciVI GTATCCnnnnn_n'

Cuts at:   1527    2288    3629    1527 
   Size:        761    1341    1527

  Fragments arranged by size: 

                1527    1341     761
 
BclI T'GATC_A

Cuts at:   1149    1149 
   Size:       3629
 
BglI GCCn_nnn'nGGC

Cuts at:    355     355 
   Size:       3629
 
BglII A'GATC_T

Cuts at:   1144    3181    1144 
   Size:       2037    1592
 
BlpI GC'TnA_GC

Cuts at:    741     741 
   Size:       3629
 
Bme1580I G_kGCm'C

Cuts at:    236    1254    1347    2403     236 
   Size:       1018      93    1056    1462

  Fragments arranged by size: 

                1462    1056    1018      93
 
BmgBI CAC'GTC

Cuts at:    198     198 
   Size:       3629
 
BmrI ACTGGGnnnn_n'

Cuts at:    841     922    1257    3401     841 
   Size:         81     335    2144    1069

  Fragments arranged by size: 

                2144    1069     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   1996    1996 
   Size:       3629
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    167     589     167 
   Size:        422    3207
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    805    1684    2176    2474     805 
   Size:        879     492     298    1960

  Fragments arranged by size: 

                1960     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    246     593    3333     246 
   Size:        347    2740     542

  Fragments arranged by size: 

                2740     542     347
 
BsaAI yAC'GTr

Cuts at:    982     982 
   Size:       3629
 
BsaBI GATnn'nnATC

Cuts at:      6     280    1143    3180       6 
   Size:        274     863    2037     455

  Fragments arranged by size: 

                2037     863     455     274
 
BsaHI Gr'CG_yC

Cuts at:   1060    1308    2010    3506    1060 
   Size:        248     702    1496    1183

  Fragments arranged by size: 

                1496    1183     702     248
 
BsaWI w'CCGG_w

Cuts at:    818    1339    2017    2291    2438    2849     818 
   Size:        521     678     274     147     411    1598

  Fragments arranged by size: 

                1598     678     521     411     274     147
 
BseYI C'CCAG_C

Cuts at:   1054    1812    2389    2885    1054 
   Size:        758     577     496    1798

  Fragments arranged by size: 

                1798     758     577     496
 
BsiEI CG_ry'CG

Cuts at:    104    1171    1217    2425     104 
   Size:       1067      46    1208    1308

  Fragments arranged by size: 

                1308    1208    1067      46
 
BsiHKAI G_wGCw'C

Cuts at:    181     503     643    1421    1611    2403     181 
   Size:        322     140     778     190     792    1407

  Fragments arranged by size: 

                1407     792     778     322     190     140
 
BsmI GAATG_Cn'

Cuts at:    557     557 
   Size:       3629
 
Bsp1286I G_dGCh'C

Cuts at:    181     236     503     643    1254    1347    1421    1611    1673 
   Size:         55     267     140     611      93      74     190      62

Cuts at:   1673    2403     181 
   Size:        730    1407

  Fragments arranged by size: 

                1407     730     611     267     190     140      93      74
                  62      55
 
BspEI T'CCGG_A

Cuts at:   2849    2849 
   Size:       3629
 
BspHI T'CATG_A

Cuts at:   2805    2805 
   Size:       3629
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    679     893    1195    1576     679 
   Size:        214     302     381    2732

  Fragments arranged by size: 

                2732     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50    1921    1975    3459    3624      50 
   Size:       1871      54    1484     165      55

  Fragments arranged by size: 

                1871    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   1541    1541 
   Size:       3629
 
BsrFI r'CCGG_y

Cuts at:   1627    1808    2017    1627 
   Size:        181     209    3239

  Fragments arranged by size: 

                3239     209     181
 
BssSI C'ACGA_G

Cuts at:   1900    2258    1900 
   Size:        358    3271
 
BstAPI GCAn_nnn'nTGC

Cuts at:   1006    1006 
   Size:       3629
 
BstBI TT'CG_AA

Cuts at:   1990    1990 
   Size:       3629
 
BstYI r'GATC_y

Cuts at:      1     654    1121    1144    1479    1725    2737    2823    2835 
   Size:        653     467      23     335     246    1012      86      12

Cuts at:   2835    3181       1 
   Size:        346     449

  Fragments arranged by size: 

                1012     653     467     449     346     335     246      86
                  23      12
 
BtgI C'CryG_G

Cuts at:    107     137     107 
   Size:         30    3599
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    146     538    1579    1760    1923     146 
   Size:        392    1041     181     163    1852

  Fragments arranged by size: 

                1852    1041     392     181     163
 
BtsI GCAGTG_nn'

Cuts at:    872     872 
   Size:       3629
 
DraI TTT'AAA

Cuts at:    164     628     164 
   Size:        464    3165
 
DrdI GACnn_nn'nnGTC

Cuts at:   1335    2193    1335 
   Size:        858    2771
 
EaeI y'GGCC_r

Cuts at:    122     156    1214    1388    1779    1806    3151     122 
   Size:         34    1058     174     391      27    1345     600

  Fragments arranged by size: 

                1345    1058     600     391     174      34      27
 
EagI C'GGCC_G

Cuts at:   1214    1214 
   Size:       3629
 
EarI CTCTTCn'nnn_

Cuts at:   1652    1862    1652 
   Size:        210    3419
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   1837    2147    2293    3439    1837 
   Size:        310     146    1146    2027

  Fragments arranged by size: 

                2027    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:    179     641     179 
   Size:        462    3167
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    167     589    1454    1886    2633    2901     167 
   Size:        422     865     432     747     268     895

  Fragments arranged by size: 

                 895     865     747     432     422     268
 
EcoO109I rG'GnC_Cy

Cuts at:    768     768 
   Size:       3629
 
EcoRI G'AATT_C

Cuts at:    633     633 
   Size:       3629
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   1648    1648 
   Size:       3629
 
FspI TGC'GCA

Cuts at:   1410    1410 
   Size:       3629
 
HaeII r_GCGC'y

Cuts at:   1063    1311    2333    3312    1063 
   Size:        248    1022     979    1380

  Fragments arranged by size: 

                1380    1022     979     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1471    1501    1503    1533    1471 
   Size:         30       2      30    3567

  Fragments arranged by size: 

                3567      30      30       2
 
HincII GTy'rAC

Cuts at:    668     668 
   Size:       3629
 
HindIII A'AGCT_T

Cuts at:    616     616 
   Size:       3629
 
KasI G'GCGC_C

Cuts at:   1059    1307    1059 
   Size:        248    3381
 
KpnI G_GTAC'C

Cuts at:    649     649 
   Size:       3629
 
MfeI C'AATT_G

Cuts at:    575     575 
   Size:       3629
 
MluI A'CGCG_T

Cuts at:   3062    3062 
   Size:       3629
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   2300    2484    2300 
   Size:        184    3445
 
MscI TGG'CCA

Cuts at:    158    1390     158 
   Size:       1232    2397
 
MslI CAynn'nnrTG

Cuts at:   1618    1745    1618 
   Size:        127    3502
 
MspA1I CmG'CkG

Cuts at:    740    1054    1414    2427    2672     740 
   Size:        314     360    1013     245    1697

  Fragments arranged by size: 

                1697    1013     360     314     245
 
NaeI GCC'GGC

Cuts at:   1810    1810 
   Size:       3629
 
NarI GG'CG_CC

Cuts at:   1060    1308    1060 
   Size:        248    3381
 
NcoI C'CATG_G

Cuts at:    107     107 
   Size:       3629
 
NgoMIV G'CCGG_C

Cuts at:   1808    1808 
   Size:       3629
 
NspI r_CATG'y

Cuts at:    682    1182    2089     682 
   Size:        500     907    2222

  Fragments arranged by size: 

                2222     907     500
 
PciI A'CATG_T

Cuts at:   2085    2085 
   Size:       3629
 
PflMI CCAn_nnn'nTGG

Cuts at:    229     229 
   Size:       3629
 
PmeI GTTT'AAAC

Cuts at:    628     628 
   Size:       3629
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2768    2800    2768 
   Size:         32    3597
 
PstI C_TGCA'G

Cuts at:    676    1361     676 
   Size:        685    2944
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       3629
 
PvuII CAG'CTG

Cuts at:   1054    1414    1054 
   Size:        360    3269
 
RsrII CG'GwC_CG

Cuts at:   1824    1824 
   Size:       3629
 
SacI G_AGCT'C

Cuts at:    181     643     181 
   Size:        462    3167
 
SalI G'TCGA_C

Cuts at:    666     666 
   Size:       3629
 
SapI GCTCTTCn'nnn_

Cuts at:   1652    1862    1652 
   Size:        210    3419
 
SbfI CC_TGCA'GG

Cuts at:    676     676 
   Size:       3629
 
ScaI AGT'ACT

Cuts at:   3146    3146 
   Size:       3629
 
SfcI C'TryA_G

Cuts at:     33     672    1357    2350    2541      33 
   Size:        639     685     993     191    1121

  Fragments arranged by size: 

                1121     993     685     639     191
 
SfoI GGC'GCC

Cuts at:   1061    1309    1061 
   Size:        248    3381
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3629
 
SmaI CCC'GGG

Cuts at:    651     651 
   Size:       3629
 
SmlI C'TyrA_G

Cuts at:    784    1699    2191    2453     784 
   Size:        915     492     262    1960

  Fragments arranged by size: 

                1960     915     492     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       3629
 
SphI G_CATG'C

Cuts at:    682    1182     682 
   Size:        500    3129
 
StyI C'CwwG_G

Cuts at:    107     763    1117     107 
   Size:        656     354    2619

  Fragments arranged by size: 

                2619     656     354
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    518    1212    1345    1804    2013     518 
   Size:        694     133     459     209    2134

  Fragments arranged by size: 

                2134     694     459     209     133
 
TatI w'GTAC_w

Cuts at:    200    3144     200 
   Size:       2944     685
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   3146    3178    3146 
   Size:         32    3597
 
Tth111I GACn'n_nGTC

Cuts at:   1426    1426 
   Size:       3629
 
XbaI T'CTAG_A

Cuts at:    660     660 
   Size:       3629
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   1124    1124 
   Size:       3629
 
XmaI C'CCGG_G

Cuts at:    649     649 
   Size:       3629
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI   AflIII     AgeI    AlwNI    ApaLI
     ApoI     AseI    AsiSI     AvaI    BamHI     BanI    BanII     BbeI
     BbsI     BcgI    BciVI     BclI     BglI    BglII     BlpI Bme1580I
    BmgBI     BmrI     BplI     BpmI    BpuEI     BsaI    BsaAI    BsaBI
    BsaHI    BsaWI    BseYI    BsiEI  BsiHKAI     BsmI Bsp1286I    BspEI
    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BssSI   BstAPI    BstBI
    BstYI     BtgI    BtgZI     BtsI     DraI     DrdI     EaeI     EagI
     EarI     EciI  EcoICRI  Eco57MI EcoO109I    EcoRI     FalI     FspI
    HaeII    Hin4I   HincII  HindIII     KasI     KpnI     MfeI     MluI
     MmeI     MscI     MslI   MspA1I     NaeI     NarI     NcoI   NgoMIV
     NspI     PciI    PflMI     PmeI     PpiI     PstI     PvuI    PvuII
    RsrII     SacI     SalI     SapI     SbfI     ScaI     SfcI     SfoI
     SgfI     SmaI     SmlI     SpeI     SphI     StyI    TaqII     TatI
     TstI  Tth111I     XbaI     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AfeI    AflII     AhdI     AleI     AlfI     AloI
     ApaI     AscI    AvrII     BaeI    BbvCI    BfrBI     BmtI   Bpu10I
    BsaXI    BseRI     BsgI    BsiWI    BsmBI    BsrGI   BssHII   BstEII
    BstXI  BstZ17I   Bsu36I     ClaI    CspCI   DraIII    EcoNI    EcoRV
     FseI    FspAI     HpaI     NdeI     NheI     NotI     NruI     NsiI
     PacI     PasI     PfoI     PmlI    PpuMI    PshAI     PsiI   PspOMI
    PspXI     PsrI    SacII    SanDI    SexAI     SfiI    SgrAI    SnaBI
     SrfI     SspI     StuI     SwaI     XhoI     XmnI     ZraI
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