Restriction map for pF1KB8708
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# NO CUT ENZYMES (  114 enzymes) #
##################################

AatII       AccI        AccII       AccIII      AclI        
AcyI        AflII       AgeI        AhdI        Alw44I      
ApaI        ApaLI       AscI        Asp700I     AspEI       
AvrII       BalI        BamHI       BbeI        BbrPI       
BbsI        BcgI        BciVI       BfrI        BglII       
BlnI        BlpI        Bpu1102I    BpuAI       BsaHI       
BsaI        BsaWI       BseAI       BsiEI       BsiWI       
BspDI       BspEI       BspHI       BssHII      BssSI       
Bst1107I    BstBI       BstEII      BstPI       BstUI       
BstYI       BstZ17I     CelII       ClaI        CpoI        
DraI        EagI        Eam1105I    EclXI       Eco52I      
EcoO65I     EcoRI       EcoRV       EcoT22I     FseI        
HgaI        Hin1I       HincII      HindII      HpaI        
KasI        KspI        MflI        MluI        MluNI       
MroI        MscI        MvnI        NaeI        NarI        
NgoMIV      NheI        NotI        NruI        NsiI        
NspV        PacI        PaeR7I      PinAI       PmaCI       
PmeI        PmlI        PshAI       Psp1406I    PstI        
PvuI        PvuII       RcaI        RsrII       SacII       
SalI        SapI        SbfI        ScaI        SfiI        
SfoI        SfuI        SgrAI       SmlI        SnaBI       
SpeI        SphI        Sse8387I    SwaI        XbaI        
XcmI        XhoI        XhoII       XmnI        


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# ONE CUT ENZYMES (   59 enzymes) #
###################################

Acc65I        940  GGTACC               G^GTACC
AcsI          235  AAATTT               [GA]^AATT[CT]
AflIII        585  ACATGT               A^C[GA][CT]GT
AocI          352  CCTGAGG              CC^T[ACGT]AGG
Aor51HI       963  AGCGCT               AGC^GCT
ApoI          235  AAATTT               [GA]^AATT[CT]
AseI          289  ATTAAT               AT^TAAT
AsnI          289  ATTAAT               AT^TAAT
Asp718I       940  GGTACC               G^GTACC
AspI          822  GACCCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaI         1312  CCCGGG               C^[CT]CG[GA]G
AviII         794  TGCGCA               TGC^GCA
BbvCI         397  GCTGAGG              CCTCAGC(-5/-2)
BclI         1782  TGATCA               T^GATCA
BfaI          850  CTAG                 C^TAG
BglI         1881  GCCTGGAAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI         127  TACGTG               [CT]AC^GT[GA]
BsaBI        1506  GATCACCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmBI        1216  GAGACG               CGTCTC(1/5)
BsmI         1623  GAATGC               GAATGC(1/-1)
BsoBI        1312  CCCGGG               C^[CT]CG[GA]G
Bsp1407I      252  TGTACA               T^GTACA
BspLU11I      585  ACATGT               A^CATGT
BsrBI         148  GAGCGG               CCGCTC(-3/-3)
BsrFI        1489  ACCGGC               [GA]^CCGG[CT]
BsrGI         252  TGTACA               T^GTACA
Bsu36I        352  CCTGAGG              CC^T[ACGT]AGG
Cfr10I       1489  ACCGGC               [GA]^CCGG[CT]
DraIII        724  CACTTCGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI          822  GACCCTGTCGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI          167  CGGCCA               [CT]^GGCC[GA]
Eco47III      963  AGCGCT               AGC^GCT
Eco81I        352  CCTGAGG              CC^T[ACGT]AGG
FauI         1028  CCCGC                CCCGC(4/6)
FbaI         1782  TGATCA               T^GATCA
FspI          794  TGCGCA               TGC^GCA
HaeII         963  AGCGCT               [GA]GCGC^[CT]
HindIII       341  AAGCTT               A^AGCTT
KpnI          940  GGTACC               GGTAC^C
MaeI          850  CTAG                 C^TAG
MamI         1506  GATCACCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI         1907  CAATTG               C^AATTG
MspA1I       1045  CAGCGG               C[AC]G^C[GT]G
MunI         1907  CAATTG               C^AATTG
NcoI         1475  CCATGG               C^CATGG
NdeI         1115  CATATG               CA^TATG
NspI          585  ACATGT               [GA]CATG^[CT]
PflFI         822  GACCCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI         723  CCACTTCGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI         289  ATTAAT               AT^TAAT
SexAI         176  ACCAGGT              A^CC[AT]GGT
SfcI          953  CTGTAG               C^T[GA][CT]AG
SmaI         1312  CCCGGG               CCC^GGG
SspBI         252  TGTACA               T^GTACA
SspI         1713  AATATT               AAT^ATT
Tth111I       822  GACCCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Van91I        723  CCACTTCGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI        1312  CCCGGG               C^CCGGG
XmaI         1312  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   18 enzymes) #
###################################

AlwI          244  GATCC                GGATC(4/5)
             1323  GATCC               
AlwNI        1045  CAGCGGCTG            CAG[ACGT][ACGT][ACGT]^CTG
             1171  CAGGACCTG           
BanI           40  GGCACC               G^G[CT][GA]CC
              940  GGTACC              
BcnI         1312  CCCGG                CC^[GC]GG
             1313  CCGGG               
BseRI         733  GAGGAG               GAGGAG(10/8)
             1543  GAGGAG              
BsgI          923  GTGCAG               GTGCAG(16/14)
             1042  GTGCAG              
BsmAI         391  GAGAC                GTCTC(1/5)
             1216  GAGAC               
BsrDI         546  CATTGC               GCAATG(2/0)
              184  GCAATG              
BstXI         122  CCAGCTACGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1139  CCATCCTGATGG        
DsaI          533  CCACGG               C^C[GA][CT]GG
             1475  CCATGG              
HapII        1313  CCGG                 C^CGG
             1490  CCGG                
HpaII        1313  CCGG                 C^CGG
             1490  CCGG                
MspI         1313  CCGG                 C^CGG
             1490  CCGG                
NciI         1312  CCCGG                CC^[GC]GG
             1313  CCGGG               
PleI          460  GACTC                GAGTC(4/5)
             1696  GAGTC               
PpuMI         820  AGGACCC              [GA]G^G[AT]CC[CT]
             1172  AGGACCT             
SacI          902  GAGCTC               GAGCT^C
             1732  GAGCTC              
StuI          401  AGGCCT               AGG^CCT
             1888  AGGCCT              


#########################################
# 3 OR MORE CUT ENZYMES (   67 enzymes) #
#########################################

AciI        AfaI        AluI        AspHI       AvaII       
BanII       BbvI        BmyI        BpmI        BsaJI       
BsiHKAI     BsiYI       BslI        BsmFI       Bsp1286I    
BspMI       BsrI        BssKI       BstAPI      BstF5I      
BstNI       Cac8I       CfoI        Cfr13I      DdeI        
DpnII       DraII       EarI        EcoNI       EcoO109I    
EcoRII      EcoT14I     Fnu4HI      FokI        HaeIII      
HhaI        HinP1I      HinfI       HphI        ItaI        
Ksp632I     MaeII       MaeIII      MboI        MboII       
MnlI        MseI        MslI        MvaI        MwoI        
NdeII       NlaIII      NlaIV       RsaI        Sau3AI      
Sau96I      ScrFI       SfaNI       StyI        TaqI        
TfiI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       TthHB8I     
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