Restriction map for pF1KB8708
 (Linear) (Six-base) MAPSORT of: KIBB8708.seq  Check: 8126  from: 1  to: 1923



KIBB8708.orf

 With 182 enzymes: SgfI * 

                        March 2, 2010 14:29  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     478    1923 
   Size:        478    1445
 
Acc65I G'GTAC_C

Cuts at:      0     940    1923 
   Size:        940     983
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1039    1607    1923 
   Size:       1039     568     316

  Fragments arranged by size: 

                1039     568     316
 
AfeI AGC'GCT

Cuts at:      0     965    1923 
   Size:        965     958
 
AflIII A'CryG_T

Cuts at:      0     585    1923 
   Size:        585    1338
 
AleI CACnn'nnGTG

Cuts at:      0     689    1923 
   Size:        689    1234
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     572     604    1923 
   Size:        572      32    1319

  Fragments arranged by size: 

                1319     572      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1050    1176    1923 
   Size:       1050     126     747

  Fragments arranged by size: 

                1050     747     126
 
ApoI r'AATT_y

Cuts at:      0     235    1923 
   Size:        235    1688
 
AseI AT'TA_AT

Cuts at:      0     290    1923 
   Size:        290    1633
 
AvaI C'yCGr_G

Cuts at:      0    1312    1923 
   Size:       1312     611
 
BanI G'GyrC_C

Cuts at:      0      40     940    1923 
   Size:         40     900     983

  Fragments arranged by size: 

                 983     900      40
 
BanII G_rGCy'C

Cuts at:      0     533     906    1024    1736    1923 
   Size:        533     373     118     712     187

  Fragments arranged by size: 

                 712     533     373     187     118
 
BbvCI CC'TCA_GC

Cuts at:      0     398    1923 
   Size:        398    1525
 
BclI T'GATC_A

Cuts at:      0    1782    1923 
   Size:       1782     141
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1887    1923 
   Size:       1887      36
 
Bme1580I G_kGCm'C

Cuts at:      0      43    1923 
   Size:         43    1880
 
BmgBI CAC'GTC

Cuts at:      0    1075    1191    1923 
   Size:       1075     116     732

  Fragments arranged by size: 

                1075     732     116
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1862    1923 
   Size:       1862      61
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     378    1923 
   Size:        378    1545
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     967    1234    1385    1768    1923 
   Size:        967     267     151     383     155

  Fragments arranged by size: 

                 967     383     267     155     151
 
Bpu10I CC'TnA_GC

Cuts at:      0     398    1534    1923 
   Size:        398    1136     389

  Fragments arranged by size: 

                1136     398     389
 
BsaAI yAC'GTr

Cuts at:      0     129    1923 
   Size:        129    1794
 
BsaBI GATnn'nnATC

Cuts at:      0    1510    1923 
   Size:       1510     413
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1795    1825    1923 
   Size:       1795      30      98

  Fragments arranged by size: 

                1795      98      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     748    1558    1923 
   Size:        748     810     365

  Fragments arranged by size: 

                 810     748     365
 
BseYI C'CCAG_C

Cuts at:      0     121     673    1222    1371    1923 
   Size:        121     552     549     149     552

  Fragments arranged by size: 

                 552     552     549     149     121
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     944    1063    1923 
   Size:        944     119     860

  Fragments arranged by size: 

                 944     860     119
 
BsiHKAI G_wGCw'C

Cuts at:      0     906    1489    1736    1923 
   Size:        906     583     247     187

  Fragments arranged by size: 

                 906     583     247     187
 
BsmI GAATG_Cn'

Cuts at:      0    1629    1923 
   Size:       1629     294
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1210    1923 
   Size:       1210     713
 
Bsp1286I G_dGCh'C

Cuts at:      0      43     533     906    1024    1489    1736    1923 
   Size:         43     490     373     118     465     247     187

  Fragments arranged by size: 

                 490     465     373     247     187     118      43
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     478    1184    1376    1923 
   Size:        478     706     192     547

  Fragments arranged by size: 

                 706     547     478     192
 
BsrBI CCG'CTC

Cuts at:      0     150    1923 
   Size:        150    1773
 
BsrDI GCAATG_nn'

Cuts at:      0     191     545    1923 
   Size:        191     354    1378

  Fragments arranged by size: 

                1378     354     191
 
BsrFI r'CCGG_y

Cuts at:      0    1489    1923 
   Size:       1489     434
 
BsrGI T'GTAC_A

Cuts at:      0     252    1923 
   Size:        252    1671
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     803    1050    1624    1923 
   Size:        803     247     574     299

  Fragments arranged by size: 

                 803     574     299     247
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     129    1146    1923 
   Size:        129    1017     777

  Fragments arranged by size: 

                1017     777     129
 
Bsu36I CC'TnA_GG

Cuts at:      0     353    1923 
   Size:        353    1570
 
BtgI C'CryG_G

Cuts at:      0     533    1475    1923 
   Size:        533     942     448

  Fragments arranged by size: 

                 942     533     448
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0      75    1497    1923 
   Size:         75    1422     426

  Fragments arranged by size: 

                1422     426      75
 
BtsI GCAGTG_nn'

Cuts at:      0     437     668    1923 
   Size:        437     231    1255

  Fragments arranged by size: 

                1255     437     231
 
DraIII CAC_nnn'GTG

Cuts at:      0     729    1923 
   Size:        729    1194
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     828    1923 
   Size:        828    1095
 
EaeI y'GGCC_r

Cuts at:      0     167    1923 
   Size:        167    1756
 
EarI CTCTTCn'nnn_

Cuts at:      0     369     908    1648    1923 
   Size:        369     539     740     275

  Fragments arranged by size: 

                 740     539     369     275
 
EcoICRI GAG'CTC

Cuts at:      0     904    1734    1923 
   Size:        904     830     189

  Fragments arranged by size: 

                 904     830     189
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     967    1039    1234    1385    1607    1768    1923 
   Size:        967      72     195     151     222     161     155

  Fragments arranged by size: 

                 967     222     195     161     155     151      72
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     807    1537    1895    1923 
   Size:        807     730     358      28

  Fragments arranged by size: 

                 807     730     358      28
 
EcoO109I rG'GnC_Cy

Cuts at:      0     821    1173    1900    1923 
   Size:        821     352     727      23

  Fragments arranged by size: 

                 821     727     352      23
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1644    1923 
   Size:       1644     279
 
FspI TGC'GCA

Cuts at:      0     796    1923 
   Size:        796    1127
 
HaeII r_GCGC'y

Cuts at:      0     967    1923 
   Size:        967     956
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     585     617    1064    1096    1923 
   Size:        585      32     447      32     827

  Fragments arranged by size: 

                 827     585     447      32      32
 
HindIII A'AGCT_T

Cuts at:      0     341    1923 
   Size:        341    1582
 
KpnI G_GTAC'C

Cuts at:      0     944    1923 
   Size:        944     979
 
MfeI C'AATT_G

Cuts at:      0    1907    1923 
   Size:       1907      16
 
MslI CAynn'nnrTG

Cuts at:      0     689    1144    1474    1923 
   Size:        689     455     330     449

  Fragments arranged by size: 

                 689     455     449     330
 
MspA1I CmG'CkG

Cuts at:      0    1047    1923 
   Size:       1047     876
 
NcoI C'CATG_G

Cuts at:      0    1475    1923 
   Size:       1475     448
 
NdeI CA'TA_TG

Cuts at:      0    1116    1923 
   Size:       1116     807
 
NspI r_CATG'y

Cuts at:      0     589    1923 
   Size:        589    1334
 
PasI CC'CwG_GG

Cuts at:      0    1206    1409    1923 
   Size:       1206     203     514

  Fragments arranged by size: 

                1206     514     203
 
PciI A'CATG_T

Cuts at:      0     585    1923 
   Size:        585    1338
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     729    1923 
   Size:        729    1194
 
PpuMI rG'GwC_Cy

Cuts at:      0     821    1173    1923 
   Size:        821     352     750

  Fragments arranged by size: 

                 821     750     352
 
SacI G_AGCT'C

Cuts at:      0     906    1736    1923 
   Size:        906     830     187

  Fragments arranged by size: 

                 906     830     187
 
SexAI A'CCwGG_T

Cuts at:      0     176    1923 
   Size:        176    1747
 
SfcI C'TryA_G

Cuts at:      0     953    1923 
   Size:        953     970
 
SmaI CCC'GGG

Cuts at:      0    1314    1923 
   Size:       1314     609
 
SspI AAT'ATT

Cuts at:      0    1715    1923 
   Size:       1715     208
 
StuI AGG'CCT

Cuts at:      0     403    1890    1923 
   Size:        403    1487      33

  Fragments arranged by size: 

                1487     403      33
 
StyI C'CwwG_G

Cuts at:      0      11    1037    1475    1839    1923 
   Size:         11    1026     438     364      84

  Fragments arranged by size: 

                1026     438     364      84      11
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     595     679     853    1422    1923 
   Size:        595      84     174     569     501

  Fragments arranged by size: 

                 595     569     501     174      84
 
TatI w'GTAC_w

Cuts at:      0     252    1923 
   Size:        252    1671
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     494     526    1795    1827    1923 
   Size:        494      32    1269      32      96

  Fragments arranged by size: 

                1269     494      96      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     825    1923 
   Size:        825    1098
 
XmaI C'CCGG_G

Cuts at:      0    1312    1923 
   Size:       1312     611
 

 Enzymes that do cut:

     AarI   Acc65I     AcuI     AfeI   AflIII     AleI     AloI    AlwNI
     ApoI     AseI     AvaI     BanI    BanII    BbvCI     BclI     BglI
 Bme1580I    BmgBI     BmrI     BplI     BpmI   Bpu10I    BsaAI    BsaBI
    BsaXI    BseRI    BseYI     BsgI  BsiHKAI     BsmI    BsmBI Bsp1286I
    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BstAPI    BstXI   Bsu36I
     BtgI    BtgZI     BtsI   DraIII     DrdI     EaeI     EarI  EcoICRI
  Eco57MI    EcoNI EcoO109I     FalI     FspI    HaeII    Hin4I  HindIII
     KpnI     MfeI     MslI   MspA1I     NcoI     NdeI     NspI     PasI
     PciI    PflMI    PpuMI     SacI    SexAI     SfcI     SmaI     SspI
     StuI     StyI    TaqII     TatI     TstI  Tth111I     XmaI

 Enzymes that do not cut: 

    AatII     AccI     AclI    AflII     AgeI     AhdI     AlfI     ApaI
    ApaLI     AscI    AsiSI    AvrII     BaeI    BamHI     BbeI     BbsI
     BcgI    BciVI    BfrBI    BglII     BlpI     BmtI    BpuEI     BsaI
    BsaHI    BsaWI    BsiEI    BsiWI    BspEI    BspHI   BssHII    BssSI
    BstBI   BstEII    BstYI  BstZ17I     ClaI    CspCI     DraI     EagI
     EciI    EcoRI    EcoRV     FseI    FspAI   HincII     HpaI     KasI
     MluI     MmeI     MscI     NaeI     NarI   NgoMIV     NheI     NotI
     NruI     NsiI     PacI     PfoI     PmeI     PmlI     PpiI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII
    SacII     SalI    SanDI     SapI     SbfI     ScaI     SfiI     SfoI
     SgfI    SgrAI     SmlI    SnaBI     SpeI     SphI     SrfI     SwaI
     XbaI     XcmI     XhoI     XmnI     ZraI
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