Restriction map for pF1KB7653
##################################
# NO CUT ENZYMES (  142 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AclI        
AflII       AflIII      AgeI        AhdI        Alw44I      
AocI        Aor51HI     ApaI        ApaLI       AscI        
AseI        AsnI        Asp718I     AspEI       AspI        
AvaI        AvaII       AviII       AvrII       BalI        
BamHI       BbrPI       BcgI        BciVI       BclI        
BfaI        BfrI        BglI        BlnI        BlpI        
Bpu1102I    BsaAI       BsaBI       BsaI        BsiEI       
BsiWI       BsmBI       BsmI        BsoBI       BspDI       
BspHI       BspLU11I    BspMI       BsrBI       BssHII      
BssSI       Bst1107I    BstBI       BstEII      BstPI       
BstUI       BstXI       BstZ17I     Bsu36I      CelII       
Cfr13I      ClaI        CpoI        DraII       DraIII      
DsaI        EaeI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      Eco81I      EcoO109I    EcoO65I     
EcoRV       EcoT14I     EcoT22I     FbaI        FseI        
FspI        HgaI        HincII      HindII      HpaI        
KpnI        KspI        MaeI        MaeII       MamI        
MfeI        MluI        MluNI       MscI        MslI        
MunI        MvnI        NcoI        NdeI        NheI        
NotI        NruI        NsiI        NspV        PacI        
PaeR7I      PflFI       PinAI       PmaCI       PmeI        
PmlI        PpuMI       PshAI       PshBI       Psp1406I    
PvuI        RcaI        RsrII       SacII       SalI        
Sau96I      SbfI        ScaI        SexAI       SfiI        
SfuI        SgrAI       SmaI        SmlI        SnaBI       
SpeI        SphI        Sse8387I    SspI        StyI        
SwaI        Tth111I     XbaI        XcmI        XhoI        
XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   54 enzymes) #
###################################

AccIII        887  TCCGGA               T^CCGGA
AcyI           13  GGCGCC               G[GA]^CG[CT]C
Asp700I       453  GAAGTTTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BanI           13  GGCGCC               G^G[CT][GA]CC
BbeI           13  GGCGCC               GGCGC^C
BbsI          862  GTCTTC               GAAGAC(2/6)
BbvCI          19  GCTGAGG              CCTCAGC(-5/-2)
BcnI          621  CCGGG                CC^[GC]GG
BglII         975  AGATCT               A^GATCT
BpuAI         862  GTCTTC               GAAGAC(2/6)
BsaHI          13  GGCGCC               G[GA]^CG[CT]C
BsaJI         959  CCCAGG               C^C[ACGT][ACGT]GG
BsaWI         887  TCCGGA               [AT]^CCGG[AT]
BseAI         887  TCCGGA               T^CCGGA
BsgI          262  GTGCAG               GTGCAG(16/14)
Bsp1407I      358  TGTACA               T^GTACA
BspEI         887  TCCGGA               T^CCGGA
BsrDI         919  GCAATG               GCAATG(2/0)
BsrFI          32  GCCGGC               [GA]^CCGG[CT]
BsrGI         358  TGTACA               T^GTACA
BstAPI        333  GCAACTCCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CfoI           14  GCGC                 GCG^C
Cfr10I         32  GCCGGC               [GA]^CCGG[CT]
DraI          241  TTTAAA               TTT^AAA
DrdI          505  GACGGTGATGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI          302  CTCTTC               CTCTTC(1/4)
EcoNI         339  CCTGCAGCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI         601  GAATTC               G^AATTC
HaeII          13  GGCGCC               [GA]GCGC^[CT]
HhaI           14  GCGC                 GCG^C
Hin1I          13  GGCGCC               G[GA]^CG[CT]C
HinP1I         14  GCGC                 G^CGC
HindIII       446  AAGCTT               A^AGCTT
HphI          508  GGTGA                GGTGA(8/7)
KasI           13  GGCGCC               G^GCGCC
Ksp632I       302  CTCTTC               CTCTTC(1/4)
MaeIII        568  GTCAC                ^GT[ACGT]AC
MroI          887  TCCGGA               T^CCGGA
NaeI           32  GCCGGC               GCC^GGC
NarI           13  GGCGCC               GG^CGCC
NciI          621  CCGGG                CC^[GC]GG
NgoMIV         32  GCCGGC               G^CCGGC
NspI          589  GCATGT               [GA]CATG^[CT]
PleI          756  GAGTC                GAGTC(4/5)
PstI          340  CTGCAG               CTGCA^G
PvuII         217  CAGCTG               CAG^CTG
SapI          301  GCTCTTC              GCTCTTC(1/4)
SfoI           13  GGCGCC               GGC^GCC
SspBI         358  TGTACA               T^GTACA
StuI           26  AGGCCT               AGG^CCT
TaqI          306  TCGA                 T^CGA
Tsp45I        568  GTCAC                ^GT[GC]AC
TthHB8I       306  TCGA                 T^CGA
XmnI          453  GAAGTTTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   18 enzymes) #
###################################

AcsI           55  GAATTT               [GA]^AATT[CT]
              601  GAATTC              
AlwI          796  GATCC                GGATC(4/5)
               78  GGATC               
AlwNI         517  CAGGAGCTG            CAG[ACGT][ACGT][ACGT]^CTG
              646  CAGCTTCTG           
ApoI           55  GAATTT               [GA]^AATT[CT]
              601  GAATTC              
BanII         891  GAGCTC               G[GA]GC[CT]^C
              964  GAGCTC              
BseRI          22  GAGGAG               GAGGAG(10/8)
              437  GAGGAG              
BsmAI         656  GAGAC                GTCTC(1/5)
              400  GTCTC               
BsmFI         116  GGGAC                GGGAC(10/14)
              705  GTCCC               
BstYI          78  GGATCT               [GA]^GATC[CT]
              975  AGATCT              
MflI           78  GGATCT               [GA]^GATC[CT]
              975  AGATCT              
MspA1I         17  CCGCTG               C[AC]G^C[GT]G
              217  CAGCTG              
NlaIV          13  GGCGCC               GG[ACGT]^[ACGT]CC
              852  GGAACC              
PflMI         516  CCAGGAGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              837  CCAGTCTGTGG         
SacI          891  GAGCTC               GAGCT^C
              964  GAGCTC              
SfcI          340  CTGCAG               C^T[GA][CT]AG
              584  CTATAG              
TfiI          526  GATTC                G^A[AT]TC
              764  GATTC               
Van91I        516  CCAGGAGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              837  CCAGTCTGTGG         
XhoII          78  GGATCT               [GA]^GATC[CT]
              975  AGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (   44 enzymes) #
#########################################

AciI        AfaI        AluI        AspHI       BbvI        
BmyI        BpmI        BsiHKAI     BsiYI       BslI        
Bsp1286I    BsrI        BssKI       BstF5I      BstNI       
Cac8I       DdeI        DpnII       EcoRII      FauI        
Fnu4HI      FokI        HaeIII      HapII       HinfI       
HpaII       ItaI        MboI        MboII       MnlI        
MseI        MspI        MvaI        MwoI        NdeII       
NlaIII      RsaI        Sau3AI      ScrFI       SfaNI       
Tru9I       TseI        Tsp509I     TspRI       
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