Restriction map for pF1KE0510
##################################
# NO CUT ENZYMES (  167 enzymes) #
##################################

AatII       AccI        AccII       AccIII      AclI        
AcyI        AflII       AflIII      AgeI        AhdI        
Alw44I      AlwI        AocI        ApaLI       AscI        
AseI        AsnI        Asp700I     AspEI       AspHI       
AspI        AvaII       AviII       AvrII       BalI        
BamHI       BbeI        BbrPI       BbsI        BbvCI       
BcgI        BciVI       BclI        BfaI        BfrI        
BglII       BlnI        BpuAI       BsaAI       BsaBI       
BsaHI       BsaWI       BseAI       BseRI       BsgI        
BsiEI       BsiHKAI     BsiWI       BsmBI       BsmFI       
Bsp1407I    BspDI       BspEI       BspHI       BspLU11I    
BspMI       BsrBI       BsrDI       BsrFI       BsrGI       
BsrI        BssHII      BssSI       Bst1107I    BstAPI      
BstBI       BstEII      BstF5I      BstPI       BstUI       
BstXI       BstYI       BstZ17I     Bsu36I      Cfr10I      
ClaI        CpoI        DraI        DraIII      DrdI        
EagI        Eam1105I    EarI        EclXI       Eco52I      
Eco81I      EcoNI       EcoO65I     EcoRI       EcoRV       
EcoT22I     FbaI        FokI        FseI        FspI        
Hin1I       HincII      HindII      HindIII     HpaI        
KasI        Ksp632I     KspI        MaeI        MaeII       
MaeIII      MamI        MfeI        MflI        MluI        
MluNI       MroI        MscI        MslI        MunI        
MvnI        NaeI        NarI        NdeI        NgoMIV      
NheI        NotI        NruI        NsiI        NspI        
NspV        PacI        PflFI       PflMI       PinAI       
PleI        PmaCI       PmeI        PmlI        PpuMI       
PshAI       PshBI       Psp1406I    PvuI        PvuII       
RcaI        RsrII       SacI        SacII       SalI        
SapI        SbfI        ScaI        SexAI       SfoI        
SfuI        SgrAI       SnaBI       SpeI        SphI        
Sse8387I    SspBI       SspI        StuI        SwaI        
Tsp45I      Tth111I     Van91I      XbaI        XcmI        
XhoII       XmnI        


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# ONE CUT ENZYMES (   35 enzymes) #
###################################

Acc65I        278  GGTACC               G^GTACC
Aor51HI       186  AGCGCT               AGC^GCT
ApaI          144  GGGCCC               GGGCC^C
Asp718I       278  GGTACC               G^GTACC
BanII         144  GGGCCC               G[GA]GC[CT]^C
BglI          137  GCCCATGGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI           75  GCTAAGC              GC^T[ACGT]AGC
BmyI          144  GGGCCC               G[AGT]GC[ACT]^C
BpmI          268  CTCCAG               CTGGAG(16/14)
Bpu1102I       75  GCTAAGC              GC^T[ACGT]AGC
BsmI          357  GCATTC               GAATGC(1/-1)
Bsp1286I      144  GGGCCC               G[AGT]GC[ACT]^C
CelII          75  GCTAAGC              GC^T[ACGT]AGC
CfoI          187  GCGC                 GCG^C
DpnII         681  GATC                 ^GATC
EaeI           46  TGGCCG               [CT]^GGCC[GA]
Eco47III      186  AGCGCT               AGC^GCT
HaeII         186  AGCGCT               [GA]GCGC^[CT]
HhaI          187  GCGC                 GCG^C
HinP1I        187  GCGC                 G^CGC
HphI          369  GGTGA                GGTGA(8/7)
KpnI          278  GGTACC               GGTAC^C
MboI          681  GATC                 ^GATC
MseI          688  TTAA                 T^TAA
MspA1I        117  CCGCTG               C[AC]G^C[GT]G
NdeII         681  GATC                 ^GATC
PaeR7I        209  CTCGAG               C^TCGAG
Sau3AI        681  GATC                 ^GATC
SfiI          136  GGCCCATGGGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI          148  CCCGGG               CCC^GGG
SmlI          209  CTCGAG               C^T[CT][GA]AG
Tru9I         688  TTAA                 T^TAA
XhoI          209  CTCGAG               C^TCGAG
XmaCI         148  CCCGGG               C^CCGGG
XmaI          148  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   24 enzymes) #
###################################

AcsI          580  GAATTT               [GA]^AATT[CT]
              638  AAATTT              
AfaI          279  GTAC                 GT^AC
              417  GTAC                
AlwNI         283  CAGCAACTG            CAG[ACGT][ACGT][ACGT]^CTG
              518  CAGAAGCTG           
ApoI          580  GAATTT               [GA]^AATT[CT]
              638  AAATTT              
AvaI          148  CCCGGG               C^[CT]CG[GA]G
              209  CTCGAG              
BanI          195  GGCACC               G^G[CT][GA]CC
              278  GGTACC              
BsaI           69  GAGACC               GGTCTC(1/5)
              326  GGTCTC              
BsoBI         148  CCCGGG               C^[CT]CG[GA]G
              209  CTCGAG              
BstNI         366  CCTGG                CC^[AT]GG
              393  CCTGG               
Cac8I          96  GCAGGC               GC[ACGT]^[ACGT]GC
              192  GCAGGC              
DraII         143  GGGGCCC              [GA]G^G[ACGT]CC[CT]
              144  GGGCCCC             
DsaI          139  CCATGG               C^C[GA][CT]GG
              432  CCATGG              
EcoO109I      143  GGGGCCC              [GA]G^G[ACGT]CC[CT]
              144  GGGCCCC             
EcoRII        366  CCTGG                ^CC[AT]GG
              393  CCTGG               
HgaI           91  GCGTC                GACGC(5/10)
              294  GCGTC               
MvaI          366  CCTGG                CC^[AT]GG
              393  CCTGG               
NcoI          139  CCATGG               C^CATGG
              432  CCATGG              
PstI          190  CTGCAG               CTGCA^G
              289  CTGCAG              
RsaI          279  GTAC                 GT^AC
              417  GTAC                
SfaNI          85  GATGC                GCATC(5/9)
              376  GATGC               
SfcI          190  CTGCAG               C^T[GA][CT]AG
              289  CTGCAG              
TaqI          210  TCGA                 T^CGA
              361  TCGA                
TspRI         178  CAGTG                CA[GC]TG[ACGT][ACGT]^
              600  CAGTG               
TthHB8I       210  TCGA                 T^CGA
              361  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   32 enzymes) #
#########################################

AciI        AluI        BbvI        BcnI        BsaJI       
BsiYI       BslI        BsmAI       BssKI       Cfr13I      
DdeI        EcoT14I     FauI        Fnu4HI      HaeIII      
HapII       HinfI       HpaII       ItaI        MboII       
MnlI        MspI        MwoI        NciI        NlaIII      
NlaIV       Sau96I      ScrFI       StyI        TfiI        
TseI        Tsp509I     
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