Restriction map for pF1KE0510
 (Linear) (Six-base) MAPSORT of: KIEE0510.seq  Check: 8004  from: 1  to: 705



KIEE0510.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:15  ..

Acc65I G'GTAC_C

Cuts at:      0     278     705 
   Size:        278     427
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     204     231     300     705 
   Size:        204      27      69     405

  Fragments arranged by size: 

                 405     204      69      27
 
AfeI AGC'GCT

Cuts at:      0     188     705 
   Size:        188     517
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     148     180     705 
   Size:        148      32     525

  Fragments arranged by size: 

                 525     148      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     288     523     705 
   Size:        288     235     182

  Fragments arranged by size: 

                 288     235     182
 
ApaI G_GGCC'C

Cuts at:      0     148     705 
   Size:        148     557
 
ApoI r'AATT_y

Cuts at:      0     580     638     705 
   Size:        580      58      67

  Fragments arranged by size: 

                 580      67      58
 
AvaI C'yCGr_G

Cuts at:      0     148     209     705 
   Size:        148      61     496

  Fragments arranged by size: 

                 496     148      61
 
BanI G'GyrC_C

Cuts at:      0     195     278     705 
   Size:        195      83     427

  Fragments arranged by size: 

                 427     195      83
 
BanII G_rGCy'C

Cuts at:      0     148     705 
   Size:        148     557
 
BglI GCCn_nnn'nGGC

Cuts at:      0     143     705 
   Size:        143     562
 
BlpI GC'TnA_GC

Cuts at:      0      76     705 
   Size:         76     629
 
Bme1580I G_kGCm'C

Cuts at:      0     148     705 
   Size:        148     557
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     253     705 
   Size:        253     452
 
Bpu10I CC'TnA_GC

Cuts at:      0     403     705 
   Size:        403     302
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      63     332     705 
   Size:         63     269     373

  Fragments arranged by size: 

                 373     269      63
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     148     178     705 
   Size:        148      30     527

  Fragments arranged by size: 

                 527     148      30
 
BsmI GAATG_Cn'

Cuts at:      0     357     705 
   Size:        357     348
 
Bsp1286I G_dGCh'C

Cuts at:      0     148     705 
   Size:        148     557
 
BtgI C'CryG_G

Cuts at:      0     139     432     705 
   Size:        139     293     273

  Fragments arranged by size: 

                 293     273     139
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0      98     705 
   Size:         98     607
 
BtsI GCAGTG_nn'

Cuts at:      0     606     705 
   Size:        606      99
 
EaeI y'GGCC_r

Cuts at:      0      46     705 
   Size:         46     659
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     204     231     253     300     705 
   Size:        204      27      22      47     405

  Fragments arranged by size: 

                 405     204      47      27      22
 
EcoO109I rG'GnC_Cy

Cuts at:      0     144     145     705 
   Size:        144       1     560

  Fragments arranged by size: 

                 560     144       1
 
HaeII r_GCGC'y

Cuts at:      0     190     705 
   Size:        190     515
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      76     108     538     570     705 
   Size:         76      32     430      32     135

  Fragments arranged by size: 

                 430     135      76      32      32
 
KpnI G_GTAC'C

Cuts at:      0     282     705 
   Size:        282     423
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     103     705 
   Size:        103     602
 
MspA1I CmG'CkG

Cuts at:      0     119     705 
   Size:        119     586
 
NcoI C'CATG_G

Cuts at:      0     139     432     705 
   Size:        139     293     273

  Fragments arranged by size: 

                 293     273     139
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     148     180     705 
   Size:        148      32     525

  Fragments arranged by size: 

                 525     148      32
 
PspOMI G'GGCC_C

Cuts at:      0     144     705 
   Size:        144     561
 
PstI C_TGCA'G

Cuts at:      0     194     293     705 
   Size:        194      99     412

  Fragments arranged by size: 

                 412     194      99
 
SfcI C'TryA_G

Cuts at:      0     190     289     705 
   Size:        190      99     416

  Fragments arranged by size: 

                 416     190      99
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0     143     705 
   Size:        143     562
 
SmaI CCC'GGG

Cuts at:      0     150     705 
   Size:        150     555
 
SmlI C'TyrA_G

Cuts at:      0     209     705 
   Size:        209     496
 
StyI C'CwwG_G

Cuts at:      0     139     432     632     705 
   Size:        139     293     200      73

  Fragments arranged by size: 

                 293     200     139      73
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     320     705 
   Size:        320     385
 
XhoI C'TCGA_G

Cuts at:      0     209     705 
   Size:        209     496
 
XmaI C'CCGG_G

Cuts at:      0     148     705 
   Size:        148     557
 

 Enzymes that do cut:

   Acc65I     AcuI     AfeI     AloI    AlwNI     ApaI     ApoI     AvaI
     BanI    BanII     BglI     BlpI Bme1580I     BpmI   Bpu10I     BsaI
    BsaXI     BsmI Bsp1286I     BtgI    BtgZI     BtsI     EaeI  Eco57MI
 EcoO109I    HaeII    Hin4I     KpnI     MmeI   MspA1I     NcoI     PpiI
   PspOMI     PstI     SfcI     SfiI     SmaI     SmlI     StyI    TaqII
     XhoI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI     AclI    AflII   AflIII     AgeI     AhdI
     AleI     AlfI    ApaLI     AscI     AseI    AsiSI    AvrII     BaeI
    BamHI     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI
    BglII    BmgBI     BmrI     BmtI     BplI    BpuEI    BsaAI    BsaBI
    BsaHI    BsaWI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI
    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI
   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI  BstZ17I
   Bsu36I     ClaI    CspCI     DraI   DraIII     DrdI     EagI     EarI
     EciI  EcoICRI    EcoNI    EcoRI    EcoRV     FalI     FseI     FspI
    FspAI   HincII  HindIII     HpaI     KasI     MfeI     MluI     MscI
     MslI     NaeI     NarI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     NspI     PacI     PasI     PciI    PflMI     PfoI     PmeI
     PmlI    PpuMI    PshAI     PsiI    PspXI     PsrI     PvuI    PvuII
    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI
    SexAI     SfoI     SgfI    SgrAI    SnaBI     SpeI     SphI     SrfI
     SspI     StuI     SwaI     TatI     TstI  Tth111I     XbaI     XcmI
     XmnI     ZraI
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