Restriction map for pF1KB6495
 (Linear) (Six-base) MAPSORT of: KIBB6495.seq  Check: 8882  from: 1  to: 855



KIBB6495.orf

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:47  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     509     855 
   Size:        509     346
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     628     660     855 
   Size:        628      32     195

  Fragments arranged by size: 

                 628     195      32
 
ApoI r'AATT_y

Cuts at:      0     274     855 
   Size:        274     581
 
AvaI C'yCGr_G

Cuts at:      0     141     855 
   Size:        141     714
 
BamHI G'GATC_C

Cuts at:      0     309     561     855 
   Size:        309     252     294

  Fragments arranged by size: 

                 309     294     252
 
BanI G'GyrC_C

Cuts at:      0      40     103     135     160     855 
   Size:         40      63      32      25     695

  Fragments arranged by size: 

                 695      63      40      32      25
 
BbeI G_GCGC'C

Cuts at:      0      44     107     139     164     855 
   Size:         44      63      32      25     691

  Fragments arranged by size: 

                 691      63      44      32      25
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     231     855 
   Size:        231     624
 
BmgBI CAC'GTC

Cuts at:      0     823     855 
   Size:        823      32
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     140     655     855 
   Size:        140     515     200

  Fragments arranged by size: 

                 515     200     140
 
Bpu10I CC'TnA_GC

Cuts at:      0     229     855 
   Size:        229     626
 
BsaHI Gr'CG_yC

Cuts at:      0      41     104     136     161     855 
   Size:         41      63      32      25     694

  Fragments arranged by size: 

                 694      63      41      32      25
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     272     302     628     658     855 
   Size:        272      30     326      30     197

  Fragments arranged by size: 

                 326     272     197      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      31     211     855 
   Size:         31     180     644

  Fragments arranged by size: 

                 644     180      31
 
BseYI C'CCAG_C

Cuts at:      0     233     686     855 
   Size:        233     453     169

  Fragments arranged by size: 

                 453     233     169
 
BsiEI CG_ry'CG

Cuts at:      0      89     131     855 
   Size:         89      42     724

  Fragments arranged by size: 

                 724      89      42
 
BsiHKAI G_wGCw'C

Cuts at:      0     398     855 
   Size:        398     457
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0      25     855 
   Size:         25     830
 
Bsp1286I G_dGCh'C

Cuts at:      0     398     855 
   Size:        398     457
 
BsrBI CCG'CTC

Cuts at:      0     146     855 
   Size:        146     709
 
BsrFI r'CCGG_y

Cuts at:      0      84     855 
   Size:         84     771
 
BsrGI T'GTAC_A

Cuts at:      0     295     855 
   Size:        295     560
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     823     855 
   Size:        823      32
 
BstYI r'GATC_y

Cuts at:      0     309     561     855 
   Size:        309     252     294

  Fragments arranged by size: 

                 309     294     252
 
BtgI C'CryG_G

Cuts at:      0      26     107     131     434     732     855 
   Size:         26      81      24     303     298     123

  Fragments arranged by size: 

                 303     298     123      81      26      24
 
DraI TTT'AAA

Cuts at:      0     257     606     855 
   Size:        257     349     249

  Fragments arranged by size: 

                 349     257     249
 
EaeI y'GGCC_r

Cuts at:      0      86     408     828     855 
   Size:         86     322     420      27

  Fragments arranged by size: 

                 420     322      86      27
 
EagI C'GGCC_G

Cuts at:      0      86     855 
   Size:         86     769
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     140     509     655     855 
   Size:        140     369     146     200

  Fragments arranged by size: 

                 369     200     146     140
 
EcoO109I rG'GnC_Cy

Cuts at:      0     378     450     855 
   Size:        378      72     405

  Fragments arranged by size: 

                 405     378      72
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     483     855 
   Size:        483     372
 
HaeII r_GCGC'y

Cuts at:      0      44     107     139     164     855 
   Size:         44      63      32      25     691

  Fragments arranged by size: 

                 691      63      44      32      25
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     135     167     855 
   Size:        135      32     688

  Fragments arranged by size: 

                 688     135      32
 
HincII GTy'rAC

Cuts at:      0     291     855 
   Size:        291     564
 
KasI G'GCGC_C

Cuts at:      0      40     103     135     160     855 
   Size:         40      63      32      25     695

  Fragments arranged by size: 

                 695      63      40      32      25
 
MscI TGG'CCA

Cuts at:      0     830     855 
   Size:        830      25
 
MslI CAynn'nnrTG

Cuts at:      0     439     855 
   Size:        439     416
 
MspA1I CmG'CkG

Cuts at:      0     109     133     764     855 
   Size:        109      24     631      91

  Fragments arranged by size: 

                 631     109      91      24
 
NaeI GCC'GGC

Cuts at:      0      86     855 
   Size:         86     769
 
NarI GG'CG_CC

Cuts at:      0      41     104     136     161     855 
   Size:         41      63      32      25     694

  Fragments arranged by size: 

                 694      63      41      32      25
 
NcoI C'CATG_G

Cuts at:      0      26     434     855 
   Size:         26     408     421

  Fragments arranged by size: 

                 421     408      26
 
NgoMIV G'CCGG_C

Cuts at:      0      84     855 
   Size:         84     771
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     628     660     855 
   Size:        628      32     195

  Fragments arranged by size: 

                 628     195      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     378     450     855 
   Size:        378      72     405

  Fragments arranged by size: 

                 405     378      72
 
SacII CC_GC'GG

Cuts at:      0     110     134     855 
   Size:        110      24     721

  Fragments arranged by size: 

                 721     110      24
 
ScaI AGT'ACT

Cuts at:      0     517     855 
   Size:        517     338
 
SfoI GGC'GCC

Cuts at:      0      42     105     137     162     855 
   Size:         42      63      32      25     693

  Fragments arranged by size: 

                 693      63      42      32      25
 
StyI C'CwwG_G

Cuts at:      0      26     434     855 
   Size:         26     408     421

  Fragments arranged by size: 

                 421     408      26
 
TatI w'GTAC_w

Cuts at:      0     295     515     796     855 
   Size:        295     220     281      59

  Fragments arranged by size: 

                 295     281     220      59
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     655     823     855 
   Size:        655     168      32

  Fragments arranged by size: 

                 655     168      32
 
XmnI GAAnn'nnTTC

Cuts at:      0     466     643     855 
   Size:        466     177     212

  Fragments arranged by size: 

                 466     212     177
 

 Enzymes that do cut:

     AcuI     AloI     ApoI     AvaI    BamHI     BanI     BbeI    BciVI
    BmgBI     BpmI   Bpu10I    BsaHI    BsaXI    BseRI    BseYI    BsiEI
  BsiHKAI    BsmBI Bsp1286I    BsrBI    BsrFI    BsrGI    BstXI    BstYI
     BtgI     DraI     EaeI     EagI  Eco57MI EcoO109I     FalI    HaeII
    Hin4I   HincII     KasI     MscI     MslI   MspA1I     NaeI     NarI
     NcoI   NgoMIV     PpiI    PpuMI    SacII     ScaI     SfoI     StyI
     TatI     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI    AlwNI     ApaI    ApaLI     AscI
     AseI    AsiSI    AvrII     BaeI    BanII     BbsI    BbvCI     BcgI
     BclI    BfrBI     BglI    BglII     BlpI Bme1580I     BmrI     BmtI
     BplI    BpuEI     BsaI    BsaAI    BsaBI    BsaWI     BsgI    BsiWI
     BsmI    BspEI    BspHI    BspMI    BsrDI   BssHII    BssSI   BstAPI
    BstBI   BstEII  BstZ17I   Bsu36I    BtgZI     BtsI     ClaI    CspCI
   DraIII     DrdI     EarI     EciI  EcoICRI    EcoNI    EcoRI    EcoRV
     FseI     FspI    FspAI  HindIII     HpaI     KpnI     MfeI     MluI
     MmeI     NdeI     NheI     NotI     NruI     NsiI     NspI     PacI
     PasI     PciI    PflMI     PfoI     PmeI     PmlI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII     SacI
     SalI    SanDI     SapI     SbfI    SexAI     SfcI     SfiI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     SwaI    TaqII     TstI  Tth111I     XbaI     XhoI     XmaI
     ZraI
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