Restriction map for pF1KB6353
 (Linear) (Six-base) MAPSORT of: KIBB6353.seq  Check: 670  from: 1  to: 798



KIBB6353.orf

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:46  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     610     798 
   Size:        610     188
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      31     798 
   Size:         31     767
 
AlwNI CAG_nnn'CTG

Cuts at:      0     411     558     798 
   Size:        411     147     240

  Fragments arranged by size: 

                 411     240     147
 
AscI GG'CGCG_CC

Cuts at:      0     300     798 
   Size:        300     498
 
BanII G_rGCy'C

Cuts at:      0      70     634     798 
   Size:         70     564     164

  Fragments arranged by size: 

                 564     164      70
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     299     798 
   Size:        299     499
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     401     798 
   Size:        401     397
 
BlpI GC'TnA_GC

Cuts at:      0      64     798 
   Size:         64     734
 
Bme1580I G_kGCm'C

Cuts at:      0     767     798 
   Size:        767      31
 
BmgBI CAC'GTC

Cuts at:      0     216     798 
   Size:        216     582
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      41     798 
   Size:         41     757
 
BsaWI w'CCGG_w

Cuts at:      0     482     798 
   Size:        482     316
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     205     798 
   Size:        205     593
 
BseYI C'CCAG_C

Cuts at:      0      85     243     505     708     798 
   Size:         85     158     262     203      90

  Fragments arranged by size: 

                 262     203     158      90      85
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     151     379     653     798 
   Size:        151     228     274     145

  Fragments arranged by size: 

                 274     228     151     145
 
BsiHKAI G_wGCw'C

Cuts at:      0      70     617     657     798 
   Size:         70     547      40     141

  Fragments arranged by size: 

                 547     141      70      40
 
Bsp1286I G_dGCh'C

Cuts at:      0      70     617     634     657     767     798 
   Size:         70     547      17      23     110      31

  Fragments arranged by size: 

                 547     110      70      31      23      17
 
BspEI T'CCGG_A

Cuts at:      0     482     798 
   Size:        482     316
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     610     798 
   Size:        610     188
 
BsrBI CCG'CTC

Cuts at:      0     153     798 
   Size:        153     645
 
BssHII G'CGCG_C

Cuts at:      0     300     798 
   Size:        300     498
 
BssSI C'ACGA_G

Cuts at:      0     790     798 
   Size:        790       8
 
Bsu36I CC'TnA_GG

Cuts at:      0     377     798 
   Size:        377     421
 
BtgI C'CryG_G

Cuts at:      0     305     798 
   Size:        305     493
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0      62      89      97     124     798 
   Size:         62      27       8      27     674

  Fragments arranged by size: 

                 674      62      27      27       8
 
EarI CTCTTCn'nnn_

Cuts at:      0     491     724     798 
   Size:        491     233      74

  Fragments arranged by size: 

                 491     233      74
 
EcoICRI GAG'CTC

Cuts at:      0      68     798 
   Size:         68     730
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      31      41     798 
   Size:         31      10     757

  Fragments arranged by size: 

                 757      31      10
 
EcoO109I rG'GnC_Cy

Cuts at:      0     401     798 
   Size:        401     397
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     375     407     618     650     798 
   Size:        375      32     211      32     148

  Fragments arranged by size: 

                 375     211     148      32      32
 
MspA1I CmG'CkG

Cuts at:      0     307     363     798 
   Size:        307      56     435

  Fragments arranged by size: 

                 435     307      56
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     558     798 
   Size:        558     240
 
PfoI T'CCnGG_A

Cuts at:      0     287     798 
   Size:        287     511
 
PpuMI rG'GwC_Cy

Cuts at:      0     401     798 
   Size:        401     397
 
PstI C_TGCA'G

Cuts at:      0     325     416     798 
   Size:        325      91     382

  Fragments arranged by size: 

                 382     325      91
 
SacI G_AGCT'C

Cuts at:      0      70     798 
   Size:         70     728
 
SacII CC_GC'GG

Cuts at:      0     308     798 
   Size:        308     490
 
SapI GCTCTTCn'nnn_

Cuts at:      0     724     798 
   Size:        724      74
 
ScaI AGT'ACT

Cuts at:      0     265     798 
   Size:        265     533
 
SfcI C'TryA_G

Cuts at:      0     321     412     798 
   Size:        321      91     386

  Fragments arranged by size: 

                 386     321      91
 
StuI AGG'CCT

Cuts at:      0     524     798 
   Size:        524     274
 
StyI C'CwwG_G

Cuts at:      0     723     798 
   Size:        723      75
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     632     794     798 
   Size:        632     162       4

  Fragments arranged by size: 

                 632     162       4
 
TatI w'GTAC_w

Cuts at:      0     263     798 
   Size:        263     535
 
XmnI GAAnn'nnTTC

Cuts at:      0     283     448     575     798 
   Size:        283     165     127     223

  Fragments arranged by size: 

                 283     223     165     127
 

 Enzymes that do cut:

     AarI     AcuI    AlwNI     AscI    BanII     BbsI    BciVI     BlpI
 Bme1580I    BmgBI     BpmI    BsaWI    BseRI    BseYI     BsgI  BsiHKAI
 Bsp1286I    BspEI    BspMI    BsrBI   BssHII    BssSI   Bsu36I     BtgI
    CspCI     EarI  EcoICRI  Eco57MI EcoO109I    Hin4I   MspA1I    PflMI
     PfoI    PpuMI     PstI     SacI    SacII     SapI     ScaI     SfcI
     StuI     StyI    TaqII     TatI     XmnI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     ApoI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI     BbeI    BbvCI
     BcgI     BclI    BfrBI     BglI    BglII     BmrI     BmtI     BplI
   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaXI    BsiEI
    BsiWI     BsmI    BsmBI    BspHI    BsrDI    BsrFI    BsrGI   BstAPI
    BstBI   BstEII    BstXI    BstYI  BstZ17I    BtgZI     BtsI     ClaI
     DraI   DraIII     DrdI     EaeI     EagI     EciI    EcoNI    EcoRI
    EcoRV     FalI     FseI     FspI    FspAI    HaeII   HincII  HindIII
     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MscI     MslI
     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     NspI     PacI     PasI     PciI     PmeI     PmlI     PpiI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI    PvuII    RsrII
     SalI    SanDI     SbfI    SexAI     SfiI     SfoI     SgfI    SgrAI
     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     SwaI
     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     ZraI
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